Papers by Marcelo Muñoz López
This article is an open access article distributed under the terms and conditions of the Creative... more This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY
Digestive Diseases and Sciences, 1999
Determination of plasma and tissue cytokinelevels in inflammatory bowel disease have frequentlyre... more Determination of plasma and tissue cytokinelevels in inflammatory bowel disease have frequentlyresulted in conflicting data. In the present study wedetermined in patients with ulcerative colitis (UC), the levels of the proinflammatory cytokinesinterleukin (IL)-1β, IL-6, interferon(IFN)-γ, and tumor-necrosis factor (TNF)-αliberated by peripheral blood mononuclear cells (PBMC)and lamina propria mononuclear cells (LPMC) after 48-hrculture with pokeweed mitogen (PWM). IL-1β, IL-6,IFN-γ and TNF-α in the supernatant weredetected by ELISA. Results show low basal levels ofIL-1β secretion by PBMC and LPMC, and a considerableincrease after mitogen stimulation. Basal IL-6production by PBMC was higher in UC patients than incontrols [2029 pg/ml, CI9 (–165 to4223) vs 572 pg/ml (–383 to 1527) respectively, P = 0.05] and also afterPWM activation [14,995 pg/ml (7759 -22230) vs 6598 pg/ml(3240-9956), respectively, P = 0.05]. In LPMC, nodifferences in IL-6 secretion were observed. TNF-α in activated PBMC of patients with UC was notsignificantly increased in relation to control (P =0.09). No constitutive secretion of IFN-γ wasobserved in mononuclear cells. IFN-γ levelssecreted by activated LPMC were lower in patients withUC than in controls [1571 pg/ml (–108 to 3251) vs7953 pg/ml (3851-12,055), respectively, P = 0.03]. Theseresults suggest that IL-6, IL-1β, and TNF-α participate as mediators in the inflammatoryphenomena observed in UC. Further studies are necessaryto evaluate the role of IFN-γ in thiscondition.
Journal of Neuroimmunology, 1995
immune function is well documented in protein calorie malnutrition (PCM). Also, central noradrene... more immune function is well documented in protein calorie malnutrition (PCM). Also, central noradrenergic hyperactivity has recently been reported in malnourished animals. Increase in central noradrenaline activity could be responsible for cell-mediated immune depression. The present study is designed to address this hypothesis by testing whether neurotoxic lesion of central noradrenergic systems by 6-hydroxydopamine (6-OHDA) could improve lymphoproliferative response to mitogens and interleukin (IL)-1 production in PCM rats. A significant enhancement of lymphoproliferative response to concanavalin A (ConA) and in IL-l production was observed in spleen mononuclear cells of PCM rats injected intracerebroventricularly with 120 pg of 6-OHDA, as compared with solvent injected and untreated PCM animals. A significant decrease in brain noradrenaline levels was produced in the drug-injected animals. These results suggest that central noradrenergic hyperactivity is one of the mechanisms involved in the immunodepression produced by malnutrition. * Corresponding author. 0165-5728/95/$09.50 0 1995 Elsevier Science B.V. All rights reserved SSDI 0165-5728(94)00151-O
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Papers by Marcelo Muñoz López