Papers by Dr. Nazish Manzoor
A gain boosting switch for S/H circuit using clock boosting and symmetrical techniques to decrease the effect of channel charge injection
2013 International Conference on Computational Problem-Solving (ICCP), 2013
A new gain boosting switch using for bottom-plate sampling in the S/H circuit that enables increa... more A new gain boosting switch using for bottom-plate sampling in the S/H circuit that enables increasing the accuracy of S/H circuit is proposed. A new method using symmetry and clock boosting techniques to remove the asymmetrical channel charge injection is also presented. Implemented in a 0.18μm CMOS process, the switch achieves ±0.3mV deviation and 924ps delay when a 5MHz square wave is sampled at 100Ms/s. The equivalent resistance ranges 4.2Ω to 7.5Ω and entire switch consumes 1.9μW at 1.8V supply when a 45MHz rail-to-rail sine wave is sampled at 100Ms/s. Particularly test results show that this new switch could be used in 10 bit, 100Ms/s pipeline ADCs.

Ecological Questions
The main object of the research was to assess the forecast values of the weather parameters by us... more The main object of the research was to assess the forecast values of the weather parameters by using three-time series methods such as Decomposition of time series, Autoregressive (AR) model with seasonal dummies and Autoregressive moving average (ARMA) /Autoregressive Integrated moving average (ARIMA) model. A recent phenomenon in weather changing has disturbed the world in general and Pakistan in particular. In Pakistan due to climate change, flood and heat stroke have taken many lives. Stationarity was measured through the Augmented Dickey-Fuller test; results showed that some variables are I(0) and some are I(1). The reliability of the forecast results was examined through the goodness of fit test. For finding the best fit model, the performance measures of various models: Root Mean Squire Error, Mean Absolute Error and Mean Absolute Percentage Error were considered. The model in which the above statistics are the minimum was chosen as the appropriate model. After model analysis...
Sarhad Journal of Agriculture

Spatial Diversity in Aspergillus niger and Yeast Cultures in Terms of Starch Saccharification and Bioethanol Production
Agricultural Research, 2022
In this study, soil and corn samples of distinct areas were used to isolate strains of A. niger a... more In this study, soil and corn samples of distinct areas were used to isolate strains of A. niger and yeast and variable colony counts per area were studied for starch saccharification and ethanol fermentation. Highest (57%) starch saccharification was from the A. niger isolated from the soil sample of Rabi, whereas Mardan Motorway Interchange was less productive in starch saccharification. The corn sample of Takhtbhai was highest (55%) in starch saccharification, in comparison to Malakhandher. Similarly, yeast strains from the soil samples of Jhandu and corn samples of Jalala produced higher ethanol distillate (29 and 19 mL) compared to the yeast cultures isolated from other areas. Corn and soil samples of Malakandher and Jalala attained highest bioethanol recovery per 100 mL of distillate, respectively. Distinct strains of A. niger and yeast present in different areas showed great diversity in saccharification and fermentation, which might be due to variations in microbial strains and the environment around them. The best of A. niger and yeast strains could be used in bioethanol and other industries as a raw material.

Comparative Performance of Carboxymethyl Cellulose as Substrate for Electricity Generation in Microbial Fuel Cell: A Review
Latin American Applied Research - An international journal, 2021
Due to the global energy crisis in the world and no proper utilization of renewable and non-renew... more Due to the global energy crisis in the world and no proper utilization of renewable and non-renewable resources, different experimental design approaches and substrates have been employed to produce bioelectricity in an MFC. The major substrate that has been tried to focus in this review paper is carboxymethyl cellulose (CMC). Carboxymethyl cellulose is an important factor in Microbial fuel cell with great importance in industry. No known enzyme is directly involved in the oxidation/reduction of CMC, however, carboxymethyl cellulases attack, specifically CMC. Moreover, our knowledge on electrochemically active bacteria is inadequate. Although, knowledge about electrochemically active bacteria is inadequate, distinct cellulose degrading bacteria have been isolated for their higher cellulase activity. Similarly, pure bacterial cultures and co-cultures have been extensively used in degrading CMC for power and electricity generation. CMC concentration and effect of different substitutio...

Natural Product Research, 2018
Using a dual culture antagonism assay, Aspergillus niger exhibited 51.5 ± 1.1 % growth inhibition... more Using a dual culture antagonism assay, Aspergillus niger exhibited 51.5 ± 1.1 % growth inhibition against Fusarium oxysporum f.sp. lycopersici, the wilt pathogen of tomato. For enhanced production of antifungal metabolites, nutrient optimization was performed and in vitro well-diffusion antifungal assays demonstrated that crude extract obtained from GPYB culture showed a maximum zone of inhibition (8.8 ± 0.4 mm) against the wilt pathogen, which is corroborated by the comparative LCMS profiles of the extracts from all three media i.e. GPYB, YEB and PDB. Two known compounds, Asperazine (m/z 665 [M+H] +) and Nigerone (m/z 571 [M+H] +), were isolated from A. niger and their antifungal activity is reported here for the first time. In MIC experiments, Asperazine and Nigerone inhibited the pathogen at 60 and 80 µg•mL-1 respectively. Molecular docking studies of Nigerone and Asperazine with F. oxysporum tomatinase showed five and six binding interactions respectively.

The Analyst, Jan 24, 2018
In this study, two slightly different halophilic mixed exoelectrogens were enriched and selected ... more In this study, two slightly different halophilic mixed exoelectrogens were enriched and selected from salt lake soils. The results showed that the selected mixed exoelectrogens ESA from the sample OSA (Xiaochaidan Lake soil) and ESB from the sample OSB (Dachaidan Lake soil), without additional NaCl, produced current densities of 1231.1 and 1050.2 μA cm-2, which were 89.6% and 61.7% higher than the typical exoelectrogen G. sulfurreducens PCA, respectively. ESA and ESB could produce 2.7 and 1.9 times higher currents than that obtained using G. sulfurreducens PCA with an additional 1.5% NaCl, respectively. The community diversity data demonstrated that Proteobacteria was the most abundant phylum, in which Enterobacteriaceae and Rhodocyclaceae were the dominant families for both ESA and ESB. Furthermore, at the genus level, the dominant genera Propionivibrio and Escherichia-Shigella were also shared by both. ESA had higher species diversity compared to ESB.

TURKISH JOURNAL OF BOTANY, 2015
seeds contain oil in extractable quantities. Therefore, algae appear to be the cheapest source am... more seeds contain oil in extractable quantities. Therefore, algae appear to be the cheapest source among all the renewable sources for biodiesel production. Green microalgae contain 20% to 70% lipid and exhibit extraordinary potential for cultivation as energy crops (Xu et al., 2006; de Vries et al., 2010). They do not need arable land for cultivation and can be grown in industrial, municipal, and agricultural effluents, and fresh-and seawater (Chinnasamy et al., 2010). The growth and oil producing efficiency of algae is much higher than that of conventional and oil seed crops such as corn and soybean (Li et al., 2008). Due to its high oil content, various countries like the UK, USA, China, Belgium, Denmark, India, and Singapore have started utilizing algae as a source of biofuels on a commercial scale. Cultivation modes and nutritional management affect the growth rate and biochemical composition of algae (Hsieh and Wu, 2009). Previous studies have demonstrated that the biomass and lipid content in some microalgae were affected by various cultivation conditions such as nitrogen supply and its sources (

The main objective of this study was to produce glucoamylase under optimum conditions and to stud... more The main objective of this study was to produce glucoamylase under optimum conditions and to study the effect of chemical mutagenesis on Aspergillus niger for the production of glucoamylase. The maximum activity of glucoamylase (3.185±0.020 IU/mL/min.) by mutant Aspergillus niger and (2.085±0.021 IU/mL/min.) for wild Aspergillus niger was recorded in the culture filtration after 96 hours of Solid State Fermentation of growth medium with 70% moisture level and in presence of 0.3% yeast extract, 0.4% peptone, and 4 mL Tween-80 at pH 4.8. The maximum fraction value after gel filtration for wild Aspergillus niger was 2.850 IU/mL/min and for mutant Aspergillus niger was 2.980 IU/mL/min. Purification through the SDS PAGE revealed the indication of glucoamylase purification from Aspergillus niger. The high value of Km shows that substrate had great affinity for glucoamylase. Glucoamylase enzyme has many useful applications in food processing industry and fermentation biotechnology.

Journal of Electroanalytical Chemistry, 2018
In this study mix culture obtained from waste water was subjected to enzyme extraction which was ... more In this study mix culture obtained from waste water was subjected to enzyme extraction which was readily used with carboxymethyl cellulose (CMC) in microbial fuel cell (MFCs) for electricity generation. The mix culture was further evaluated for dominant bacterial strain and individual enzyme activity. It was observed that the higher current density was recorded with dialyzed enzyme (360 μA cm-2) than control (without enzyme) 307 μA cm-2. Similarly power density of (MFCs) was also high in the dialyzed enzyme (866 ± 16.6 mW m-2) as compared to control (737 ± 2.0 mW m-2). Sequencing and relative abundance analysis of bacterial diversity in bacterial mix culture and the biofilm of dialyzed enzyme added system showed Proteobacteria, Bacteriodetes and Firmicutes as dominant phyla among the microbial communities. Citrobacter amalonaticus and Tolumonas osonensis OCF7 were isolated and evaluated for their enzyme activity which showed activity values of 0.67 IU mL-1 min-1 and 0.5 IU mL-1 min-1 respectively. These results demonstrated that microbial
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Papers by Dr. Nazish Manzoor