Bulletin archéologique des Écoles françaises à l’étranger
Ce document a été généré automatiquement le 28 juillet 2021. Le Bulletin archéologique des Écoles... more Ce document a été généré automatiquement le 28 juillet 2021. Le Bulletin archéologique des Écoles françaises à l'étranger est mise à disposition selon les termes de la Licence Creative Commons Attribution-Pas d'Utilisation Commerciale-Pas de Modification 4.0 International.
Chicken astroviruses (CAstV) were associated with retarded growth, enteritis, kidney diseases, an... more Chicken astroviruses (CAstV) were associated with retarded growth, enteritis, kidney diseases, and white chick syndrome. In the current study, we aimed to evaluate the effect of CAstV infection on growth, performance, and gross and histopathological picture of commercial chicken flocks suffering increased culling rate and decreased performance. Samples were collected for virus isolation, identification, and sequencing on day one, 15 days, and 30 days of age. Body weight, feed conversion rate, and mortality rates were determined. A gross examination was performed, and tissue samples from the liver, intestine, kidneys, heart, and lungs were kept in formalin for histopathological evaluation. Embryos inoculated with CAstV revealed dwarfism, and edema. The cytopathic effect on CAstV inoculated cells included aggregation,, and sloughing. The isolated Egyptian isolates shared the highest nucleotide homology (93%) with the Korean isolate Kr/ADL102655-1/2010 and showed the most distant relat...
The current worldwide COVID-19 pandemic is causing severe human health problems, with high mortal... more The current worldwide COVID-19 pandemic is causing severe human health problems, with high mortality rates and huge economic burdens requiring the urgent development of a safe and effective vaccine. Here, preclinical evaluation of an inactivated SARS-CoV-2 vaccine candidate (EgySerVac-20) is reported. Oropharyngeal swabs and nasopharyngeal aspirates obtained from Egyptian patients with laboratory-confirmed SARS-CoV-2 infection were isolated using Vero cells and were then genetically characterized. Vaccine inactivation was performed using diluted formaldehyde, followed by safety testing for the inactivated vaccine. To determine the high humoral immune responses against SARSCOV-2 infection, the safety and capacity of the vaccine prepared with alum adjuvant were tested. The immunogenicity and efficacy of the vaccine candidate was tested in vitro by a neutralization assay and in vivo using mouse models. Our results revealed a cytopathic effect which was observed 48 hours post infection ...
In the present study, the antiviral activity of Curcumin (Curcuma longa) and bottle gourd (Lagena... more In the present study, the antiviral activity of Curcumin (Curcuma longa) and bottle gourd (Lagenaria siceraria) extracts was evaluated against the field and vaccine strains of Marek's disease virus (MDV). Plaque reduction assays were performed and the obtained results showed that both Curcumin and bottle gourd had a reduction effect on the number of plaques in comparison with the control vaccines. Curcumin exhibited prominent antiviral effect expressed as 80% and 66% plaque reduction percent (PR%) against HVT and Rispen's respectively at the 1:8 virus dilution. While, bottle gourd has moderate antiviral action at 1:4 dilution; it has 40% and 33% PR% against HVT and Rispen's respectively at dilution 1:8. The obtained results were evaluated using polymerase chain reaction PCR SYBR Mix and showed antiviral activity of these plants against Marek's disease virus as Curcumin 26.1±1.7a, Bottle gourd 25.62 ±1.1a, Positive control 17.125 ±0.6b; these results indicate that both Curcumin and bottle gourd have an antiviral effect against recently isolated wild type MDV.
The Tianlai project is a 21cm intensity mapping experiment aimed at detecting dark energy by meas... more The Tianlai project is a 21cm intensity mapping experiment aimed at detecting dark energy by measuring the baryon acoustic oscillation (BAO) features in the large scale structure power spectrum. This experiment provides an opportunity to test the data processing methods for cosmological 21cm signal extraction, which is still a great challenge in current radio astronomy research. The 21cm signal is much weaker than the foregrounds and easily affected by the imperfections in the instrumental responses. Furthermore, processing the large volumes of interferometer data poses a practical challenge. We have developed a data processing pipeline software called tlpipe to process the drift scan survey data from the Tianlai experiment. It performs offline data processing tasks such as radio frequency interference (RFI) flagging, array calibration, binning, and map-making, etc. It also includes utility functions needed for the data analysis, such as data selection, transformation, visualization and others. A number of new algorithms are implemented, for example the eigenvector decomposition method for array calibration and the Tikhonov regularization for m-mode analysis. In this paper we describe the design and implementation of the tlpipe and illustrate its functions with some analysis of real data. Finally, we outline directions for future development of this publicly code.
Infectious bronchitis virus is an acute contagious disease cause severe economic losses in poultr... more Infectious bronchitis virus is an acute contagious disease cause severe economic losses in poultry production worldwide. Virus isolation is the golden standard for IBV diagnosis so the aim of this study was comparing the different biological systems for isolation of infectious bronchitis virus. The Egyptian varian2 IBV strain representative to the current circulating strain in Egypt was isolated in SPF-ECE, chicken embryo kidney cell (CEK) and in vero cell line and was confirmed for IBV detection by Reverse transcriptase Real time PCR (RRT-PCR) using IB specific primers. Comparison of different isolation methods was done by generating a standard curve by real time PCR. The results revealed that The propagated virus in CEK showed the highest virus titer (5.952 x 106 and 3.245 x 106) than vero cell line (7.184 x 101 and 2.14 x 103) and ECE (7.536 x 103 and 5.444 x 105) after both 48 and 72 hours respectively.
Fowl adenoviruses (FAdVs) are a large group of viruses of different serotypes. They are responsib... more Fowl adenoviruses (FAdVs) are a large group of viruses of different serotypes. They are responsible for inclusion body hepatitis, adenoviral gizzard erosion, and hepatitis hydropericardium syndrome. The present study presents a comprehensive overview of FAdVs in Egypt, with a focus on the epidemiological features of virus serotypes across the country. We conducted molecular investigation of multiple FAdV species based on the genetic signature of hypervariable regions 1–4 in the loop1 (L1) region of the hexon gene. Epidemiologically, the Nile Delta governorates showed high positivity of FAdVs, which were more commonly found in broilers than in layers. Genetically, species D and serotype 8a/E dominated, and the findings also revealed the emergence of new FAdV serotypes 1, 3, and 8b. The comparative analysis of hypervariable regions in the L1 region of the hexon gene revealed variables specific to each virus serotype. In silico predictions of L1 region revealed variations in the molecular structure and predicted the antigenic epitopes which may affect the cross-antigenicity between the different FAdV species and serotypes.
Zywnosc Nauka Technologia Jakosc/Food Science Technology Quality, 2019
Prognozowanie mikrobiologiczne polega na założeniu powtarzalności odpowiedzi populacji drobnoustr... more Prognozowanie mikrobiologiczne polega na założeniu powtarzalności odpowiedzi populacji drobnoustrojów na zadane czynniki środowiska produktu żywnościowego. Na podstawie danych empirycznych pochodzących z doświadczeń mikrobiologicznych opracowywane są modele prognostyczne jako programy komputerowe bądź bazy danych dostępne on-line. Celem pracy było utworzenie bazy danych modeli prognostycznych bakterii potencjalnie probiotycznych, saprofitycznych i patogennych w formie użytecznego dla odbiorcy końcowego narzędzia zainstalowanego na serwerze SGGW pod nazwą ProgBaz SGGW. Materiał do badań stanowiły matematyczne modele wzrostu i przeżywalności bakterii saprofitycznych (psychrotrofów, Pseudomonas spp., drożdzy i pleśni) i patogennych: Salmonella spp., Staphylococcus aureus, Listeria monocytogenes (mikroflory niekorzystnej) oraz potencjalnie probiotycznych szczepów z rodzaju Lactobacillus (mikroflory korzystnej). Modele opracowano w Zakładzie Higieny i Zarządzania Jakością Żywności Wydzia...
Introduction: The novel coronavirus severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) ... more Introduction: The novel coronavirus severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has spread throughout the globe, causing a pandemic. In Egypt over 115,000 individuals were infected so far. Objective: In the present study, the objective is to perform a complete genome sequence of SAR-CoV2 isolated from Egyptian coronavirus disease (COVID-19) patients. Methods: Nasopharyngeal swabs were collected from 61 COVID-19 patients who attended at National Cancer Institute, Kasr Al-Aini Hospital and the army hospital. Viral RNA was extracted and whole genomic sequencing was conducted using Next Generation Sequencing. Results: In all cases, the sequenced virus has at least 99% identity to the reference Wuhan 1. The sequence analysis showed 204 distinct genome variations including 114 missense mutations, 72 synonymous mutations, 1 disruptive in-frame deletion, 7 downstream gene mutations, 6 upstream gene mutations, 3 frame-shift deletions, and 1 in-frame deletion. The most dominant clades were G/GH/GR/O and the dominant type is B.
The current worldwide pandemic COVID-19 is causing severe human health problems, with high number... more The current worldwide pandemic COVID-19 is causing severe human health problems, with high numbers of mortality rates and huge economic burdens that require an urgent demand for safe, and effective and vaccine development. Our study was the first trail to development and evaluation of safety and immune response to inactivated whole SARS-COV-2 virus vaccine adjuvanted with aluminium hydroxide. We used characterized SARS-COV-2 strain, severe acute respiratory syndrome coronavirus 2 isolates (SARS-CoV-2/human/EGY/Egy-SERVAC/2020) with accession numbers; MT981440; MT981439; MT981441; MT974071; MT974069 and MW250352 at GenBank that isolated from Egyptian patients SARS-CoV-2-positive. Development of the vaccine was carried out in a BSL - 3 facilities and the immunogenicity was determined in mice at two doses (55µg and 100µg per dose). All vaccinated mice were received a booster dose 14 days post first immunization. Our results demonstrated distinct cytopathic effect on the vero cell monol...
Aim: To accomplish a comparative study between two types of attachment Equator and Ball/Socket in... more Aim: To accomplish a comparative study between two types of attachment Equator and Ball/Socket in mandibular implant supported overdenture by using finite element analysis and assessment of stress distribution on the bone, implant. overdenture, mucosa and attachment. Methodology: Three-dimensional models simulated a clinical situation where an edentulous mandible was restored by two different treatment options using implant overdentures. The overdentures were supported by two implants placed at the approximate canine region using Ball/Socket and Equator attachments. The load was applied of value of 50,100,200 N with angle of vertical 90° then the oblique load with angel 45°. Results: Attachment total deformation with using ball and socket attachments deforms much more than using equator attachment. Similarly, attachments Von Mises stress increase dramatically with ball and socket attachment. Central incisor loading did not show as much difference as molar area loading. Conclusion: Equators body and its caps (flexible and metallic) received lower stresses that indicating longer life time and longer periods between successive maintenances.
Background Helicobacter pylori (H. Pylori) infection and hyperglycemia may be associated with an ... more Background Helicobacter pylori (H. Pylori) infection and hyperglycemia may be associated with an increased risk of colorectal neoplasm. However these two factors affect colorectal neoplasm remain controversial. We aimed to carry out a meta-analysis to evaluate the study population diabetes prevalence rate and H. pylori infection rate with colorectal adenoma risk. Methods We conducted a systemic research through English databases for medical reports. We also recorded the diabetes prevalence and H. pylori infection prevalence in each study. We classified these studies into 4 subgroups as their background population diabetes prevalence < 6%(Group 1); between 6 to 8%(Group 2); between 8 to 10%(Group 3) and more than 10%(Group 4). The random effects model had used to calculate pooled prevalence estimates with 95% confidence interval [CI]. Results Twenty seven studies were finally eligible for meta-analysis. The random-effects model of meta-analysis was chosen, showing pooled odds rati...
The characterization of the newly emerged variant strains in the Egyptian field is important for ... more The characterization of the newly emerged variant strains in the Egyptian field is important for improving the control measures for avian infectious bronchitis virus (IBV) and understanding the cause of recent outbreaks. Four isolates of IBV were selected from the isolates bank of the Reference Laboratory for Veterinary Quality Control on Poultry Production (RLQP) from different provinces during 2010 and 2012. We conducted a comparison between the selected field isolates sequences, other Egyptian isolates published on the gene bank and the vaccinal strains of the commercial vaccines that are commonly used in Egypt. The sequencing of full length S1 gene (Spike 1 glycoprotein) was done and phylogenetic tree of Egyptian viruses was constructed; also nucleotide and amino acid identities were calculated. The results of phylogenetic tree of the Egyptian viruses showed that the Egyptian IBV can be grouped into three main groups (classic, IS/1494 like strains and new variant group).The amin...
2014 XXXIth URSI General Assembly and Scientific Symposium (URSI GASS), 2014
The Tianlai project plans to build a compact cylinder array to map the 21cm emission from redshif... more The Tianlai project plans to build a compact cylinder array to map the 21cm emission from redshift 0 to 3, corresponding to frequency 350-1420MHz. The full array is designed to have a size of about 100m-100m in dimension. A site with minimum radio frequency interference (RFI) and convenience of logisitics support is desired. This project is also constrained by its low budget, so attention have to be paid to reduce the cost for the construction and operation. We have conducted site surveys in many places in China, obtained radio spectrum measurement at more than a hundred potential site locations. This paper describes the site selection and survey method. We have tentatively selected a site in Hongliuxia, Balikun County, Xinjiang Autonomous Region, which have very low RFI and is potentially good for other low frequency radio experiments.
Monthly Notices of the Royal Astronomical Society: Letters, 2013
The large-scale distribution of neutral hydrogen in the Universe will be luminous through its 21 ... more The large-scale distribution of neutral hydrogen in the Universe will be luminous through its 21 cm emission. Here, for the first time, we use the auto-power spectrum of 21 cm intensity fluctuations to constrain neutral hydrogen fluctuations at z ∼ 0.8. Our data were acquired with the Green Bank Telescope and span the redshift range 0.6 < z < 1 over two fields totalling ≈41 deg 2 and 190 h of radio integration time. The dominant synchrotron foregrounds exceed the signal by ∼10 3 , but have fewer degrees of freedom and can be removed efficiently. Even in the presence of residual foregrounds, the auto-power can still be interpreted as an upper bound on the 21 cm signal. Our previous measurements of the cross-correlation of 21 cm intensity and the WiggleZ galaxy survey provide a lower bound. Through a Bayesian treatment of signal and foregrounds, we can combine both fields in auto-and cross-power into a measurement of H I b H I = [0.62 +0.23 −0.15 ] × 10 −3 at 68 per cent confidence with 9 per cent systematic calibration uncertainty, where H I is the neutral hydrogen (H I) fraction and b H I is the H I bias parameter. We describe observational challenges with the present data set and plans to overcome them.
Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression, 2002
[Fe]-hydrogenases are redoxenzymes that catalyze the reversible reduction of protons to hydrogen.... more [Fe]-hydrogenases are redoxenzymes that catalyze the reversible reduction of protons to hydrogen. Hydrogenase activity was observed in a culture of the unicellular green alga Chlorella fusca after an anaerobic incubation, but not in the related species Chlorella vulgaris. Specific polymerase chain reaction (PCR) techniques lead to the isolation of the cDNA and the genomic DNA of a special type of
Avian influenza due to highly pathogenic avian influenza (HPAIV) H5N1 virus is not a food-borne i... more Avian influenza due to highly pathogenic avian influenza (HPAIV) H5N1 virus is not a food-borne illness but a serious panzootic disease with the potential to be pandemic. In this study, broiler chickens were vaccinated with commercial H5N1 or H5N2 inactivated vaccines prior to being challenged with an HPAIV H5N1 (clade 2.2.1 classic) virus. Challenged and non-challenged vaccinated chickens were kept together, and unvaccinated chickens served as contact groups. Post-challenge samples from skin and edible internal organs were collected from dead and sacrificed (after a 14-day observation period) birds and tested using qRT-PCR for virus detection and quantification. H5N1 vaccine protected chickens against morbidity, mortality and transmission. Virus RNA was not detected in the meat or edible organs of chickens vaccinated with H5N1 vaccine. Conversely, H5N2 vaccine did not confer clinical protection, and a significant virus load was detected in the meat and internal organs. Phylogenetic analysis showed that the H5N1 virus vaccine and challenge virus strains are closely related. The results of the present study strongly suggest a need for proper selection of vaccines and their routine evaluation against newly emergent field viruses. These actions will help to reduce human exposure to HPAIV H5N1 virus from both infected live birds and slaughtered poultry. In addition, rigorous preventive measures should be put in place in order to minimize the public-health risks of avian influenza at the human-animal interface.
Bulletin archéologique des Écoles françaises à l’étranger
Ce document a été généré automatiquement le 28 juillet 2021. Le Bulletin archéologique des Écoles... more Ce document a été généré automatiquement le 28 juillet 2021. Le Bulletin archéologique des Écoles françaises à l'étranger est mise à disposition selon les termes de la Licence Creative Commons Attribution-Pas d'Utilisation Commerciale-Pas de Modification 4.0 International.
Chicken astroviruses (CAstV) were associated with retarded growth, enteritis, kidney diseases, an... more Chicken astroviruses (CAstV) were associated with retarded growth, enteritis, kidney diseases, and white chick syndrome. In the current study, we aimed to evaluate the effect of CAstV infection on growth, performance, and gross and histopathological picture of commercial chicken flocks suffering increased culling rate and decreased performance. Samples were collected for virus isolation, identification, and sequencing on day one, 15 days, and 30 days of age. Body weight, feed conversion rate, and mortality rates were determined. A gross examination was performed, and tissue samples from the liver, intestine, kidneys, heart, and lungs were kept in formalin for histopathological evaluation. Embryos inoculated with CAstV revealed dwarfism, and edema. The cytopathic effect on CAstV inoculated cells included aggregation,, and sloughing. The isolated Egyptian isolates shared the highest nucleotide homology (93%) with the Korean isolate Kr/ADL102655-1/2010 and showed the most distant relat...
The current worldwide COVID-19 pandemic is causing severe human health problems, with high mortal... more The current worldwide COVID-19 pandemic is causing severe human health problems, with high mortality rates and huge economic burdens requiring the urgent development of a safe and effective vaccine. Here, preclinical evaluation of an inactivated SARS-CoV-2 vaccine candidate (EgySerVac-20) is reported. Oropharyngeal swabs and nasopharyngeal aspirates obtained from Egyptian patients with laboratory-confirmed SARS-CoV-2 infection were isolated using Vero cells and were then genetically characterized. Vaccine inactivation was performed using diluted formaldehyde, followed by safety testing for the inactivated vaccine. To determine the high humoral immune responses against SARSCOV-2 infection, the safety and capacity of the vaccine prepared with alum adjuvant were tested. The immunogenicity and efficacy of the vaccine candidate was tested in vitro by a neutralization assay and in vivo using mouse models. Our results revealed a cytopathic effect which was observed 48 hours post infection ...
In the present study, the antiviral activity of Curcumin (Curcuma longa) and bottle gourd (Lagena... more In the present study, the antiviral activity of Curcumin (Curcuma longa) and bottle gourd (Lagenaria siceraria) extracts was evaluated against the field and vaccine strains of Marek's disease virus (MDV). Plaque reduction assays were performed and the obtained results showed that both Curcumin and bottle gourd had a reduction effect on the number of plaques in comparison with the control vaccines. Curcumin exhibited prominent antiviral effect expressed as 80% and 66% plaque reduction percent (PR%) against HVT and Rispen's respectively at the 1:8 virus dilution. While, bottle gourd has moderate antiviral action at 1:4 dilution; it has 40% and 33% PR% against HVT and Rispen's respectively at dilution 1:8. The obtained results were evaluated using polymerase chain reaction PCR SYBR Mix and showed antiviral activity of these plants against Marek's disease virus as Curcumin 26.1±1.7a, Bottle gourd 25.62 ±1.1a, Positive control 17.125 ±0.6b; these results indicate that both Curcumin and bottle gourd have an antiviral effect against recently isolated wild type MDV.
The Tianlai project is a 21cm intensity mapping experiment aimed at detecting dark energy by meas... more The Tianlai project is a 21cm intensity mapping experiment aimed at detecting dark energy by measuring the baryon acoustic oscillation (BAO) features in the large scale structure power spectrum. This experiment provides an opportunity to test the data processing methods for cosmological 21cm signal extraction, which is still a great challenge in current radio astronomy research. The 21cm signal is much weaker than the foregrounds and easily affected by the imperfections in the instrumental responses. Furthermore, processing the large volumes of interferometer data poses a practical challenge. We have developed a data processing pipeline software called tlpipe to process the drift scan survey data from the Tianlai experiment. It performs offline data processing tasks such as radio frequency interference (RFI) flagging, array calibration, binning, and map-making, etc. It also includes utility functions needed for the data analysis, such as data selection, transformation, visualization and others. A number of new algorithms are implemented, for example the eigenvector decomposition method for array calibration and the Tikhonov regularization for m-mode analysis. In this paper we describe the design and implementation of the tlpipe and illustrate its functions with some analysis of real data. Finally, we outline directions for future development of this publicly code.
Infectious bronchitis virus is an acute contagious disease cause severe economic losses in poultr... more Infectious bronchitis virus is an acute contagious disease cause severe economic losses in poultry production worldwide. Virus isolation is the golden standard for IBV diagnosis so the aim of this study was comparing the different biological systems for isolation of infectious bronchitis virus. The Egyptian varian2 IBV strain representative to the current circulating strain in Egypt was isolated in SPF-ECE, chicken embryo kidney cell (CEK) and in vero cell line and was confirmed for IBV detection by Reverse transcriptase Real time PCR (RRT-PCR) using IB specific primers. Comparison of different isolation methods was done by generating a standard curve by real time PCR. The results revealed that The propagated virus in CEK showed the highest virus titer (5.952 x 106 and 3.245 x 106) than vero cell line (7.184 x 101 and 2.14 x 103) and ECE (7.536 x 103 and 5.444 x 105) after both 48 and 72 hours respectively.
Fowl adenoviruses (FAdVs) are a large group of viruses of different serotypes. They are responsib... more Fowl adenoviruses (FAdVs) are a large group of viruses of different serotypes. They are responsible for inclusion body hepatitis, adenoviral gizzard erosion, and hepatitis hydropericardium syndrome. The present study presents a comprehensive overview of FAdVs in Egypt, with a focus on the epidemiological features of virus serotypes across the country. We conducted molecular investigation of multiple FAdV species based on the genetic signature of hypervariable regions 1–4 in the loop1 (L1) region of the hexon gene. Epidemiologically, the Nile Delta governorates showed high positivity of FAdVs, which were more commonly found in broilers than in layers. Genetically, species D and serotype 8a/E dominated, and the findings also revealed the emergence of new FAdV serotypes 1, 3, and 8b. The comparative analysis of hypervariable regions in the L1 region of the hexon gene revealed variables specific to each virus serotype. In silico predictions of L1 region revealed variations in the molecular structure and predicted the antigenic epitopes which may affect the cross-antigenicity between the different FAdV species and serotypes.
Zywnosc Nauka Technologia Jakosc/Food Science Technology Quality, 2019
Prognozowanie mikrobiologiczne polega na założeniu powtarzalności odpowiedzi populacji drobnoustr... more Prognozowanie mikrobiologiczne polega na założeniu powtarzalności odpowiedzi populacji drobnoustrojów na zadane czynniki środowiska produktu żywnościowego. Na podstawie danych empirycznych pochodzących z doświadczeń mikrobiologicznych opracowywane są modele prognostyczne jako programy komputerowe bądź bazy danych dostępne on-line. Celem pracy było utworzenie bazy danych modeli prognostycznych bakterii potencjalnie probiotycznych, saprofitycznych i patogennych w formie użytecznego dla odbiorcy końcowego narzędzia zainstalowanego na serwerze SGGW pod nazwą ProgBaz SGGW. Materiał do badań stanowiły matematyczne modele wzrostu i przeżywalności bakterii saprofitycznych (psychrotrofów, Pseudomonas spp., drożdzy i pleśni) i patogennych: Salmonella spp., Staphylococcus aureus, Listeria monocytogenes (mikroflory niekorzystnej) oraz potencjalnie probiotycznych szczepów z rodzaju Lactobacillus (mikroflory korzystnej). Modele opracowano w Zakładzie Higieny i Zarządzania Jakością Żywności Wydzia...
Introduction: The novel coronavirus severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) ... more Introduction: The novel coronavirus severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has spread throughout the globe, causing a pandemic. In Egypt over 115,000 individuals were infected so far. Objective: In the present study, the objective is to perform a complete genome sequence of SAR-CoV2 isolated from Egyptian coronavirus disease (COVID-19) patients. Methods: Nasopharyngeal swabs were collected from 61 COVID-19 patients who attended at National Cancer Institute, Kasr Al-Aini Hospital and the army hospital. Viral RNA was extracted and whole genomic sequencing was conducted using Next Generation Sequencing. Results: In all cases, the sequenced virus has at least 99% identity to the reference Wuhan 1. The sequence analysis showed 204 distinct genome variations including 114 missense mutations, 72 synonymous mutations, 1 disruptive in-frame deletion, 7 downstream gene mutations, 6 upstream gene mutations, 3 frame-shift deletions, and 1 in-frame deletion. The most dominant clades were G/GH/GR/O and the dominant type is B.
The current worldwide pandemic COVID-19 is causing severe human health problems, with high number... more The current worldwide pandemic COVID-19 is causing severe human health problems, with high numbers of mortality rates and huge economic burdens that require an urgent demand for safe, and effective and vaccine development. Our study was the first trail to development and evaluation of safety and immune response to inactivated whole SARS-COV-2 virus vaccine adjuvanted with aluminium hydroxide. We used characterized SARS-COV-2 strain, severe acute respiratory syndrome coronavirus 2 isolates (SARS-CoV-2/human/EGY/Egy-SERVAC/2020) with accession numbers; MT981440; MT981439; MT981441; MT974071; MT974069 and MW250352 at GenBank that isolated from Egyptian patients SARS-CoV-2-positive. Development of the vaccine was carried out in a BSL - 3 facilities and the immunogenicity was determined in mice at two doses (55µg and 100µg per dose). All vaccinated mice were received a booster dose 14 days post first immunization. Our results demonstrated distinct cytopathic effect on the vero cell monol...
Aim: To accomplish a comparative study between two types of attachment Equator and Ball/Socket in... more Aim: To accomplish a comparative study between two types of attachment Equator and Ball/Socket in mandibular implant supported overdenture by using finite element analysis and assessment of stress distribution on the bone, implant. overdenture, mucosa and attachment. Methodology: Three-dimensional models simulated a clinical situation where an edentulous mandible was restored by two different treatment options using implant overdentures. The overdentures were supported by two implants placed at the approximate canine region using Ball/Socket and Equator attachments. The load was applied of value of 50,100,200 N with angle of vertical 90° then the oblique load with angel 45°. Results: Attachment total deformation with using ball and socket attachments deforms much more than using equator attachment. Similarly, attachments Von Mises stress increase dramatically with ball and socket attachment. Central incisor loading did not show as much difference as molar area loading. Conclusion: Equators body and its caps (flexible and metallic) received lower stresses that indicating longer life time and longer periods between successive maintenances.
Background Helicobacter pylori (H. Pylori) infection and hyperglycemia may be associated with an ... more Background Helicobacter pylori (H. Pylori) infection and hyperglycemia may be associated with an increased risk of colorectal neoplasm. However these two factors affect colorectal neoplasm remain controversial. We aimed to carry out a meta-analysis to evaluate the study population diabetes prevalence rate and H. pylori infection rate with colorectal adenoma risk. Methods We conducted a systemic research through English databases for medical reports. We also recorded the diabetes prevalence and H. pylori infection prevalence in each study. We classified these studies into 4 subgroups as their background population diabetes prevalence < 6%(Group 1); between 6 to 8%(Group 2); between 8 to 10%(Group 3) and more than 10%(Group 4). The random effects model had used to calculate pooled prevalence estimates with 95% confidence interval [CI]. Results Twenty seven studies were finally eligible for meta-analysis. The random-effects model of meta-analysis was chosen, showing pooled odds rati...
The characterization of the newly emerged variant strains in the Egyptian field is important for ... more The characterization of the newly emerged variant strains in the Egyptian field is important for improving the control measures for avian infectious bronchitis virus (IBV) and understanding the cause of recent outbreaks. Four isolates of IBV were selected from the isolates bank of the Reference Laboratory for Veterinary Quality Control on Poultry Production (RLQP) from different provinces during 2010 and 2012. We conducted a comparison between the selected field isolates sequences, other Egyptian isolates published on the gene bank and the vaccinal strains of the commercial vaccines that are commonly used in Egypt. The sequencing of full length S1 gene (Spike 1 glycoprotein) was done and phylogenetic tree of Egyptian viruses was constructed; also nucleotide and amino acid identities were calculated. The results of phylogenetic tree of the Egyptian viruses showed that the Egyptian IBV can be grouped into three main groups (classic, IS/1494 like strains and new variant group).The amin...
2014 XXXIth URSI General Assembly and Scientific Symposium (URSI GASS), 2014
The Tianlai project plans to build a compact cylinder array to map the 21cm emission from redshif... more The Tianlai project plans to build a compact cylinder array to map the 21cm emission from redshift 0 to 3, corresponding to frequency 350-1420MHz. The full array is designed to have a size of about 100m-100m in dimension. A site with minimum radio frequency interference (RFI) and convenience of logisitics support is desired. This project is also constrained by its low budget, so attention have to be paid to reduce the cost for the construction and operation. We have conducted site surveys in many places in China, obtained radio spectrum measurement at more than a hundred potential site locations. This paper describes the site selection and survey method. We have tentatively selected a site in Hongliuxia, Balikun County, Xinjiang Autonomous Region, which have very low RFI and is potentially good for other low frequency radio experiments.
Monthly Notices of the Royal Astronomical Society: Letters, 2013
The large-scale distribution of neutral hydrogen in the Universe will be luminous through its 21 ... more The large-scale distribution of neutral hydrogen in the Universe will be luminous through its 21 cm emission. Here, for the first time, we use the auto-power spectrum of 21 cm intensity fluctuations to constrain neutral hydrogen fluctuations at z ∼ 0.8. Our data were acquired with the Green Bank Telescope and span the redshift range 0.6 < z < 1 over two fields totalling ≈41 deg 2 and 190 h of radio integration time. The dominant synchrotron foregrounds exceed the signal by ∼10 3 , but have fewer degrees of freedom and can be removed efficiently. Even in the presence of residual foregrounds, the auto-power can still be interpreted as an upper bound on the 21 cm signal. Our previous measurements of the cross-correlation of 21 cm intensity and the WiggleZ galaxy survey provide a lower bound. Through a Bayesian treatment of signal and foregrounds, we can combine both fields in auto-and cross-power into a measurement of H I b H I = [0.62 +0.23 −0.15 ] × 10 −3 at 68 per cent confidence with 9 per cent systematic calibration uncertainty, where H I is the neutral hydrogen (H I) fraction and b H I is the H I bias parameter. We describe observational challenges with the present data set and plans to overcome them.
Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression, 2002
[Fe]-hydrogenases are redoxenzymes that catalyze the reversible reduction of protons to hydrogen.... more [Fe]-hydrogenases are redoxenzymes that catalyze the reversible reduction of protons to hydrogen. Hydrogenase activity was observed in a culture of the unicellular green alga Chlorella fusca after an anaerobic incubation, but not in the related species Chlorella vulgaris. Specific polymerase chain reaction (PCR) techniques lead to the isolation of the cDNA and the genomic DNA of a special type of
Avian influenza due to highly pathogenic avian influenza (HPAIV) H5N1 virus is not a food-borne i... more Avian influenza due to highly pathogenic avian influenza (HPAIV) H5N1 virus is not a food-borne illness but a serious panzootic disease with the potential to be pandemic. In this study, broiler chickens were vaccinated with commercial H5N1 or H5N2 inactivated vaccines prior to being challenged with an HPAIV H5N1 (clade 2.2.1 classic) virus. Challenged and non-challenged vaccinated chickens were kept together, and unvaccinated chickens served as contact groups. Post-challenge samples from skin and edible internal organs were collected from dead and sacrificed (after a 14-day observation period) birds and tested using qRT-PCR for virus detection and quantification. H5N1 vaccine protected chickens against morbidity, mortality and transmission. Virus RNA was not detected in the meat or edible organs of chickens vaccinated with H5N1 vaccine. Conversely, H5N2 vaccine did not confer clinical protection, and a significant virus load was detected in the meat and internal organs. Phylogenetic analysis showed that the H5N1 virus vaccine and challenge virus strains are closely related. The results of the present study strongly suggest a need for proper selection of vaccines and their routine evaluation against newly emergent field viruses. These actions will help to reduce human exposure to HPAIV H5N1 virus from both infected live birds and slaughtered poultry. In addition, rigorous preventive measures should be put in place in order to minimize the public-health risks of avian influenza at the human-animal interface.
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