Aquatic saline ecosystems are suitable environments for isolation of microorganisms with high div... more Aquatic saline ecosystems are suitable environments for isolation of microorganisms with high diversity and widely used biotechnology features. The pectinase enzyme is one of the most important commercial enzymes with high potential in food and pharmaceutical industries. Therefore, the discovery of microorganisms with new characteristics has always been a focus of research. As such, a pectinase producing actinomycete Streptomyces coelicoflavus GIAL86 was isolated from Meyghan Salt Lake of Arak located in Markazi Province of Iran. This strain was screened among 35 isolates of halotolerant actinomycetes with the highest production of pectinase enzymes. It was also found that production of pectate lyase, pectin esterase and polygalacturonase increased simultaneously with the logarithmic growth of the strain and its maximum production is at the time of stationary phase beginning. Also, some actinomycete strains with more pectinase activity were identified by molecular identification and...
Background & aim: Hydatidosis is a zoonotic disease caused by infection with the larvae of Echino... more Background & aim: Hydatidosis is a zoonotic disease caused by infection with the larvae of Echinococcus granulosus. Different antigens produced in larval stage of this parasite that recombinant vaccine base these antigens created significant immunity in infected animals. One of the important antigens is p14-3-3 that its recombinant antigen created considerable immunity in mouse models. In the present study, according to the high immunity of antigen epitopes region, the coding sequence of T-cell epitopes of P14-3-3 was cloned into pEGFP-N1vector in order to produce an effective DNA vaccine model to stimulate high level of Th1 immune response. Methods: In the current study, bioinformatics tools were used for the prediction of linear T-Cell epitopes of Echinococcus granulosus P14-3-3 antigen. The nucleotide coding sequence of these epitopes was synthesized by PCR. The ampliqon was digested with XhoI restriction enzyme and cloned into pEGFP-N1 vector which had been purificated by modified by the Sambrook method with CaCl2 and PEG6000.Positive colony was selected by direct colony PCR and confirmed by the sequencing. The evaluation of its expression in Eukaryotic cells was done by transformed of CHO cell line with electroporation. Results: Linear T-cell epitopes of Echinococcus granulosus P14-3-3 after prediction, synthesis and amplification were successfully cloned into pEGFP-N1 vector that purificated by new method with maximum vector and minimum RNA concentration. The expression of new construct in CHO cell line as a eukaryotic cells achievement by fluorescent microscope will be used as a DNA vaccine model for the evaluation of immuno response in mouse models. Conclusion: Successfully cloning of the linear T-cell epitppes coding sequence of Echinococcus granulosus P14-3-3 antigen into pEGFP-N1 verified by sequencing and fluorscent microscope images demonstrated the expression of recombinant protein in CHO cell line.
Abstract Background Recent research has revealed the substantial role of MicroRNAs as a hallmark ... more Abstract Background Recent research has revealed the substantial role of MicroRNAs as a hallmark of some diseases such as cancer. These molecules belong to a unique category of endogenous, small non-coding RNAs which can have an effect on regulating gene expression by degrading mRNAs or suppressing translation. They play an important role as regulators of tumor suppressors and oncogenes in pathological processes. Single Nucleotide Polymorphisms (SNP) which is located in the miRNAcoding genes may contribute to the process of the development of various cancers, including breast cancer. This process occurs by altering the expression of mature miRNA. Former studies have observed the association of miRNA SNPs with susceptibility to breast cancer. Objective Our study evaluated the effect of miR196-a2 polymorphism (rs11614913) on patients suffering from breast cancer in south of Iran. Methods and results In this research, the hsa-miR-196a2 rs11614913 SNP was examined in 200 Iranian women including 100 patients suffering from breast cancer and 100 healthy women. The SNP was determined by RFLP-PCR. The X2 or Fisher's exact test was used to compare the control and the experimental groups. Statistical analysis of SNPs frequencies showed no significant difference among the patients and the control group regarding the distribution of genotype (P = 0.831). Conclusions This study's results indicated that the TT polymorphic genotype in hsa-miR-196a2 rs11614913 SNP was not related to breast cancer risk among Southern Iranian patients.
Pediatric neurorehabilitation research center, social welfare and rehabilitation sciences univers... more Pediatric neurorehabilitation research center, social welfare and rehabilitation sciences university, Iran
The advent of techniques for global analyses of cell biology, such as genomics and proteomics, op... more The advent of techniques for global analyses of cell biology, such as genomics and proteomics, opens the way to rapid progress in understanding the molecular control of developing tissues. However, such studies in the CNS are hindered by the complexity of this tissue. In particular, few approaches allow cells to be isolated that are enriched for specific stages of their
The advent of techniques for global analyses of cell biology, such as genomics and proteomics, op... more The advent of techniques for global analyses of cell biology, such as genomics and proteomics, opens the way to rapid progress in understanding the molecular control of developing tissues. However, such studies in the CNS are hindered by the complexity of this tissue. In particular, few approaches allow cells to be isolated that are enriched for specific stages of their maturation. We describe a new strategy to study gene expression and function in cerebellar granule cells. In these experiments, we have used square pulse electroporation to introduce fluorescent dye or DNA constructs into immature granule cell precursors in situ. This method only labels granule cell precursors in the superficial part of the external granule layer. Combining this labelling with fluorescent activated cell sorting (FACS) allows the transfected cells to be isolated at any time during their subsequent development, thus providing a means of analysing granule cells as they undergo maturation. This transfection method can be used to study events in the normal maturation of granule cells or the effects of introduced transgenes. Such studies can be carried out on cells purified from primary cultures or cells in situ using cerebellar slice cultures. Our strategy provides a new route to detailed analysis of the role of genes in controlling many aspects of granule cell biology. These approaches will allow recent global analyses to be more readily applied to subpopulations of cells in complex tissues.
Aquatic saline ecosystems are suitable environments for isolation of microorganisms with high div... more Aquatic saline ecosystems are suitable environments for isolation of microorganisms with high diversity and widely used biotechnology features. The pectinase enzyme is one of the most important commercial enzymes with high potential in food and pharmaceutical industries. Therefore, the discovery of microorganisms with new characteristics has always been a focus of research. As such, a pectinase producing actinomycete Streptomyces coelicoflavus GIAL86 was isolated from Meyghan Salt Lake of Arak located in Markazi Province of Iran. This strain was screened among 35 isolates of halotolerant actinomycetes with the highest production of pectinase enzymes. It was also found that production of pectate lyase, pectin esterase and polygalacturonase increased simultaneously with the logarithmic growth of the strain and its maximum production is at the time of stationary phase beginning. Also, some actinomycete strains with more pectinase activity were identified by molecular identification and...
Background & aim: Hydatidosis is a zoonotic disease caused by infection with the larvae of Echino... more Background & aim: Hydatidosis is a zoonotic disease caused by infection with the larvae of Echinococcus granulosus. Different antigens produced in larval stage of this parasite that recombinant vaccine base these antigens created significant immunity in infected animals. One of the important antigens is p14-3-3 that its recombinant antigen created considerable immunity in mouse models. In the present study, according to the high immunity of antigen epitopes region, the coding sequence of T-cell epitopes of P14-3-3 was cloned into pEGFP-N1vector in order to produce an effective DNA vaccine model to stimulate high level of Th1 immune response. Methods: In the current study, bioinformatics tools were used for the prediction of linear T-Cell epitopes of Echinococcus granulosus P14-3-3 antigen. The nucleotide coding sequence of these epitopes was synthesized by PCR. The ampliqon was digested with XhoI restriction enzyme and cloned into pEGFP-N1 vector which had been purificated by modified by the Sambrook method with CaCl2 and PEG6000.Positive colony was selected by direct colony PCR and confirmed by the sequencing. The evaluation of its expression in Eukaryotic cells was done by transformed of CHO cell line with electroporation. Results: Linear T-cell epitopes of Echinococcus granulosus P14-3-3 after prediction, synthesis and amplification were successfully cloned into pEGFP-N1 vector that purificated by new method with maximum vector and minimum RNA concentration. The expression of new construct in CHO cell line as a eukaryotic cells achievement by fluorescent microscope will be used as a DNA vaccine model for the evaluation of immuno response in mouse models. Conclusion: Successfully cloning of the linear T-cell epitppes coding sequence of Echinococcus granulosus P14-3-3 antigen into pEGFP-N1 verified by sequencing and fluorscent microscope images demonstrated the expression of recombinant protein in CHO cell line.
Abstract Background Recent research has revealed the substantial role of MicroRNAs as a hallmark ... more Abstract Background Recent research has revealed the substantial role of MicroRNAs as a hallmark of some diseases such as cancer. These molecules belong to a unique category of endogenous, small non-coding RNAs which can have an effect on regulating gene expression by degrading mRNAs or suppressing translation. They play an important role as regulators of tumor suppressors and oncogenes in pathological processes. Single Nucleotide Polymorphisms (SNP) which is located in the miRNAcoding genes may contribute to the process of the development of various cancers, including breast cancer. This process occurs by altering the expression of mature miRNA. Former studies have observed the association of miRNA SNPs with susceptibility to breast cancer. Objective Our study evaluated the effect of miR196-a2 polymorphism (rs11614913) on patients suffering from breast cancer in south of Iran. Methods and results In this research, the hsa-miR-196a2 rs11614913 SNP was examined in 200 Iranian women including 100 patients suffering from breast cancer and 100 healthy women. The SNP was determined by RFLP-PCR. The X2 or Fisher's exact test was used to compare the control and the experimental groups. Statistical analysis of SNPs frequencies showed no significant difference among the patients and the control group regarding the distribution of genotype (P = 0.831). Conclusions This study's results indicated that the TT polymorphic genotype in hsa-miR-196a2 rs11614913 SNP was not related to breast cancer risk among Southern Iranian patients.
Pediatric neurorehabilitation research center, social welfare and rehabilitation sciences univers... more Pediatric neurorehabilitation research center, social welfare and rehabilitation sciences university, Iran
The advent of techniques for global analyses of cell biology, such as genomics and proteomics, op... more The advent of techniques for global analyses of cell biology, such as genomics and proteomics, opens the way to rapid progress in understanding the molecular control of developing tissues. However, such studies in the CNS are hindered by the complexity of this tissue. In particular, few approaches allow cells to be isolated that are enriched for specific stages of their
The advent of techniques for global analyses of cell biology, such as genomics and proteomics, op... more The advent of techniques for global analyses of cell biology, such as genomics and proteomics, opens the way to rapid progress in understanding the molecular control of developing tissues. However, such studies in the CNS are hindered by the complexity of this tissue. In particular, few approaches allow cells to be isolated that are enriched for specific stages of their maturation. We describe a new strategy to study gene expression and function in cerebellar granule cells. In these experiments, we have used square pulse electroporation to introduce fluorescent dye or DNA constructs into immature granule cell precursors in situ. This method only labels granule cell precursors in the superficial part of the external granule layer. Combining this labelling with fluorescent activated cell sorting (FACS) allows the transfected cells to be isolated at any time during their subsequent development, thus providing a means of analysing granule cells as they undergo maturation. This transfection method can be used to study events in the normal maturation of granule cells or the effects of introduced transgenes. Such studies can be carried out on cells purified from primary cultures or cells in situ using cerebellar slice cultures. Our strategy provides a new route to detailed analysis of the role of genes in controlling many aspects of granule cell biology. These approaches will allow recent global analyses to be more readily applied to subpopulations of cells in complex tissues.
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Papers by M Tahmaseb