Article type : Original Article What is behind phylogenetic analysis of hospital, community and l... more Article type : Original Article What is behind phylogenetic analysis of hospital, community and livestock associated methicillin-resistant Staphylococcus aureus?
Differentiation of closely related methicillin resistant staphylococcus aureus (MRSA) isolated fr... more Differentiation of closely related methicillin resistant staphylococcus aureus (MRSA) isolated from food chain is required for epidemiological and food safety studies. This study provided evidence for using coagulase (coa) gene sequence repeats as a powerful diagnostic tool for differentiation of closely MRSA isolated from different sources. Staphylococcal species were isolated from milk, meat, and meat-products. Staphylococcus aureus strains with identical antibiograms were differentiated using enterotoxin genotyping, coa gene polymorphism, the patterns of polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) of coa and nucleotides sequencing of coa gene-PCR products. Nine CoPs and MRSA strains were closely related and could not be differentiated by antibiogram. Six strains of them were differentiated by enterotoxin genotyping and PCR-RFLP of coa gene and three strains were accurately differentiated by nucleotides sequencing of coa gene-PCR products. In conclusion, the above findings highlight the promising role of coa repeat region nucleotide sequences in differentiation of closely related MRSA in food chain that can help in their diagnosis and epidemiological studies.
The increased resistance of vancomycin among methicillin resistant Staphylococcus aureus (MRSA) h... more The increased resistance of vancomycin among methicillin resistant Staphylococcus aureus (MRSA) has produced a major formidable threat in the therapeutic field. The current study analyzed the vancomycin resistance traits among MRSA isolates recovered from 148 samples of animal and human origins in Sharkia Governorate, Egypt. All staphylococci isolates were examined against 8 antimicrobials and vancomycin minimal inhibitory concentrations (MICs) were then determined among phenotypic vancomycin resistant and intermediate S. aureus . Furthermore, all vancomycin-resistant S. aureus (VRSA) isolates were exposed to PCR analysis of mec A and van genes. Herein, 45 of 86 Staphylococcus spp. were identified as S. aureus , while 41 were coagulase negative staphylococci (CoNS). A higher incidence rate of S. aureus was observed in meat products (58.06%), but majority of CoNS isolates were isolated from milk samples (54.54%) with no statistical differences ( P < 0.05) in the distribution of S....
Cellular and molecular biology (Noisy-le-Grand, France), Jan 26, 2015
The present study was designed to elucidate the phenotypic and genotypic characterization of S. a... more The present study was designed to elucidate the phenotypic and genotypic characterization of S. aureus isolates in Egypt. The antibiotic susceptibility pattern of 133 identified S. aureus isolates revealed that over 70% of the isolates were multi-drug resistant (MDR). Fifty MDR isolates were characterized using antibiotyping and different molecular typing methods. Amplification of mecA gene confirmed 30 strains as methicillin-resistant S. aureus (MRSA) and 20 as methicillin-sensitive S. aureus (MSSA). Generally, 22 MRSA (73.33%) and ten MSSA (50%) harbored Panton-Valentine leukocidin (pvl) gene with no statistically significant difference (p=0.093). Staphylococcal cassette chromosome (SCC) mec typing revealed that 48% of the typeable MRSA isolates possessed SCCmec type IV. SCCmec and antimicrobial susceptibility typing signified the presence of community-acquired (CA)-MRSA in Egypt. Surprisingly, all isolates of SCCmec types I, II and III and 50% of both MRSA isolates of SCCmec type...
The present study was designed to elucidate the phenotypic and genotypic characterization of S. a... more The present study was designed to elucidate the phenotypic and genotypic characterization of S. aureus isolates in Egypt. The antibiotic susceptibility pattern of 133 identified S. aureus isolates revealed that over 70% of the isolates were multi-drug resistant (MDR). Fifty MDR isolates were characterized using antibiotyping and different molecular typing methods. Amplification of mecA gene confirmed 30 strains as methicillin-resistant S. aureus (MRSA) and 20 as methicillin-sensitive S. aureus (MSSA). Generally, 22 MRSA (73.33%) and ten MSSA (50%) harbored Panton-Valentine leukocidin (pvl) gene with no statistically significant difference (p=0.093). Staphylococcal cassette chromosome (SCC) mec typing revealed that 48% of the typeable MRSA isolates possessed SCCmec type IV. SCCmec and anti-microbial susceptibility typing signified the presence of community-acquired (CA)-MRSA in Egypt. Surprisingly, all isolates of SCCmec types I, II and III and 50% of both MRSA isolates of SCCmec types IV and V possessed pvl gene. It was clear that staphylococcal protein A (spa) and coagulase (coa) typing discriminated the isolates into eight different groups, whilst polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) of coagulase gene yielded ten distinct RFLP banding patterns. This study presented the first baseline information on the characterization of MRSA and MSSA in Egypt.
Staphylococcus aureus is a persistent human and animal pathogen responsible for a large spectrum ... more Staphylococcus aureus is a persistent human and animal pathogen responsible for a large spectrum of infections due to the production of secreted and cell surface-associated proteins under regulation of the agr (accessory gene regulator) locus. This study was undertaken to find out the prevalence of Staphylococcus species in Sharkia province, Egypt in 2012 and to investigate the potential association between agr groups and the occurrence of toxin genes among S. aureus isolates. In the present study, 46 Staphylococcus species were isolated from 141 different samples (32.62%), 26 were identified as S. aureus and 20 were classified as coagulase negative staphylococci (CoNS). In particular, 15 S. aureus strains identified phenotypically were confirmed genotypically by polymerase chain reaction (PCR) amplification of thermonuclease (nuc) and coagulase (coa) genes. All of them possessed nuc gene and were grouped into 15 different groups according to coa gene polymorphism. Moreover, typing of the agr operon revealed the predominance of agr group I (93.33%). Interestingly, all 15 S. aureus isolates tested were toxin producers with a high prevalence of staphylococcal enterotoxin A (sea) gene (86.67%). None of the isolates harbored the genes encoding seb, sec, sed and exfoliative toxin B (etb). All sea and tst (toxic shock syndrome toxin) positive strains were of agr type I. Overall, toxin and coagulase genotyping could be more discriminatory than agr typing of the isolates. Finally, we can speculate that the preferential association between certain agr alleles and certain toxin genes may make the activation of virulence factors more efficient.
Article type : Original Article What is behind phylogenetic analysis of hospital, community and l... more Article type : Original Article What is behind phylogenetic analysis of hospital, community and livestock associated methicillin-resistant Staphylococcus aureus?
Differentiation of closely related methicillin resistant staphylococcus aureus (MRSA) isolated fr... more Differentiation of closely related methicillin resistant staphylococcus aureus (MRSA) isolated from food chain is required for epidemiological and food safety studies. This study provided evidence for using coagulase (coa) gene sequence repeats as a powerful diagnostic tool for differentiation of closely MRSA isolated from different sources. Staphylococcal species were isolated from milk, meat, and meat-products. Staphylococcus aureus strains with identical antibiograms were differentiated using enterotoxin genotyping, coa gene polymorphism, the patterns of polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) of coa and nucleotides sequencing of coa gene-PCR products. Nine CoPs and MRSA strains were closely related and could not be differentiated by antibiogram. Six strains of them were differentiated by enterotoxin genotyping and PCR-RFLP of coa gene and three strains were accurately differentiated by nucleotides sequencing of coa gene-PCR products. In conclusion, the above findings highlight the promising role of coa repeat region nucleotide sequences in differentiation of closely related MRSA in food chain that can help in their diagnosis and epidemiological studies.
The increased resistance of vancomycin among methicillin resistant Staphylococcus aureus (MRSA) h... more The increased resistance of vancomycin among methicillin resistant Staphylococcus aureus (MRSA) has produced a major formidable threat in the therapeutic field. The current study analyzed the vancomycin resistance traits among MRSA isolates recovered from 148 samples of animal and human origins in Sharkia Governorate, Egypt. All staphylococci isolates were examined against 8 antimicrobials and vancomycin minimal inhibitory concentrations (MICs) were then determined among phenotypic vancomycin resistant and intermediate S. aureus . Furthermore, all vancomycin-resistant S. aureus (VRSA) isolates were exposed to PCR analysis of mec A and van genes. Herein, 45 of 86 Staphylococcus spp. were identified as S. aureus , while 41 were coagulase negative staphylococci (CoNS). A higher incidence rate of S. aureus was observed in meat products (58.06%), but majority of CoNS isolates were isolated from milk samples (54.54%) with no statistical differences ( P < 0.05) in the distribution of S....
Cellular and molecular biology (Noisy-le-Grand, France), Jan 26, 2015
The present study was designed to elucidate the phenotypic and genotypic characterization of S. a... more The present study was designed to elucidate the phenotypic and genotypic characterization of S. aureus isolates in Egypt. The antibiotic susceptibility pattern of 133 identified S. aureus isolates revealed that over 70% of the isolates were multi-drug resistant (MDR). Fifty MDR isolates were characterized using antibiotyping and different molecular typing methods. Amplification of mecA gene confirmed 30 strains as methicillin-resistant S. aureus (MRSA) and 20 as methicillin-sensitive S. aureus (MSSA). Generally, 22 MRSA (73.33%) and ten MSSA (50%) harbored Panton-Valentine leukocidin (pvl) gene with no statistically significant difference (p=0.093). Staphylococcal cassette chromosome (SCC) mec typing revealed that 48% of the typeable MRSA isolates possessed SCCmec type IV. SCCmec and antimicrobial susceptibility typing signified the presence of community-acquired (CA)-MRSA in Egypt. Surprisingly, all isolates of SCCmec types I, II and III and 50% of both MRSA isolates of SCCmec type...
The present study was designed to elucidate the phenotypic and genotypic characterization of S. a... more The present study was designed to elucidate the phenotypic and genotypic characterization of S. aureus isolates in Egypt. The antibiotic susceptibility pattern of 133 identified S. aureus isolates revealed that over 70% of the isolates were multi-drug resistant (MDR). Fifty MDR isolates were characterized using antibiotyping and different molecular typing methods. Amplification of mecA gene confirmed 30 strains as methicillin-resistant S. aureus (MRSA) and 20 as methicillin-sensitive S. aureus (MSSA). Generally, 22 MRSA (73.33%) and ten MSSA (50%) harbored Panton-Valentine leukocidin (pvl) gene with no statistically significant difference (p=0.093). Staphylococcal cassette chromosome (SCC) mec typing revealed that 48% of the typeable MRSA isolates possessed SCCmec type IV. SCCmec and anti-microbial susceptibility typing signified the presence of community-acquired (CA)-MRSA in Egypt. Surprisingly, all isolates of SCCmec types I, II and III and 50% of both MRSA isolates of SCCmec types IV and V possessed pvl gene. It was clear that staphylococcal protein A (spa) and coagulase (coa) typing discriminated the isolates into eight different groups, whilst polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) of coagulase gene yielded ten distinct RFLP banding patterns. This study presented the first baseline information on the characterization of MRSA and MSSA in Egypt.
Staphylococcus aureus is a persistent human and animal pathogen responsible for a large spectrum ... more Staphylococcus aureus is a persistent human and animal pathogen responsible for a large spectrum of infections due to the production of secreted and cell surface-associated proteins under regulation of the agr (accessory gene regulator) locus. This study was undertaken to find out the prevalence of Staphylococcus species in Sharkia province, Egypt in 2012 and to investigate the potential association between agr groups and the occurrence of toxin genes among S. aureus isolates. In the present study, 46 Staphylococcus species were isolated from 141 different samples (32.62%), 26 were identified as S. aureus and 20 were classified as coagulase negative staphylococci (CoNS). In particular, 15 S. aureus strains identified phenotypically were confirmed genotypically by polymerase chain reaction (PCR) amplification of thermonuclease (nuc) and coagulase (coa) genes. All of them possessed nuc gene and were grouped into 15 different groups according to coa gene polymorphism. Moreover, typing of the agr operon revealed the predominance of agr group I (93.33%). Interestingly, all 15 S. aureus isolates tested were toxin producers with a high prevalence of staphylococcal enterotoxin A (sea) gene (86.67%). None of the isolates harbored the genes encoding seb, sec, sed and exfoliative toxin B (etb). All sea and tst (toxic shock syndrome toxin) positive strains were of agr type I. Overall, toxin and coagulase genotyping could be more discriminatory than agr typing of the isolates. Finally, we can speculate that the preferential association between certain agr alleles and certain toxin genes may make the activation of virulence factors more efficient.
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Papers by M. M. Bendary