Mónica Quito

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Armando Marques-Guedes

UNL - New University of Lisbon

Carlos G. Wagner

Universidad Complutense de Madrid

Frédérique Langue G.

Centre National de la Recherche Scientifique / French National Centre for Scientific Research

Arturo Sanchez Sanz

Universidad Complutense de Madrid

Cuadernos de Literatura

Pontificia Universidad Javeriana

Salo Carvalho

Universidade Federal do Rio de Janeiro (UFRJ)

Lorena Amorós Blasco

Universidad de Murcia

Pablo Sanchez-Ostiz

Universidad de Navarra

Tulio Vianna

UFMG - The Federal University of Minas Gerais

Tomás Mantecón

Universidad de Cantabria

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$Research paper thumbnail of Microarray analysis for identification of Plasmodium -refractoriness candidate genes in mosquitoes$

Microarray analysis for identification of Plasmodium -refractoriness candidate genes in mosquitoes

Genome, 2004

The identification and cloning of genes conferring mosquito refractoriness to the malaria parasit... more The identification and cloning of genes conferring mosquito refractoriness to the malaria parasite is critical for understanding malaria transmission mechanisms and holds great promise for developing novel approaches to malaria control. The mosquito midgut is the first major site of interaction between the parasite and the mosquito. Failure of the parasite to negotiate this environment can be a barrier for development and is likely the main cause of mosquito refractoriness. This paper reports a study on Aedes aegypti midgut expressed sequence tag (EST) identification and the determination of genes differentially expressed in mosquito populations susceptible and refractory to the avian malaria parasite Plasmodium gallinaceum. We sequenced a total of 1200 cDNA clones and obtained 1183 high-quality mosquito midgut ESTs that were computationally collapsed into 105 contigs and 251 singlets. All 1200 midgut cDNA clones, together with an additional 102 genetically or physically mapped Ae. aegypti clones, were spotted on single arrays with 12 replicates. Of those interrogated microarray elements, 28 (2.3%) were differentially expressed between the susceptible and refractory mosquito populations. Twenty-seven elements showed at least a two-fold increase in expression in the susceptible population level relative to the refractory population and one clone showed reduced expression. Sequence analysis of these differentially expressed genes revealed that 10 showed no significant similarity to any known genes, 6 clones had matches with unannotated genes of Anopheles gambiae, and 12 clones exhibited significant similarity to known genes. Real-time quantitative RT-PCR of selected clones confirmed the mRNA expression profiles from the microarray analysis.

LAS ESTRATEGIAS

Contenidos 1. Introducción 2. ¿Qué es el método de proyectos? 3. Cómo se organiza el método de pr... more

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LAS ESTRATEGIAS

Contenidos 1. Introducción 2. ¿Qué es el método de proyectos? 3. Cómo se organiza el método de pr... more

Download

$Research paper thumbnail of Microarray analysis for identification of Plasmodium -refractoriness candidate genes in mosquitoes$

Microarray analysis for identification of Plasmodium -refractoriness candidate genes in mosquitoes

Genome, 2004

LAS ESTRATEGIAS

Contenidos 1. Introducción 2. ¿Qué es el método de proyectos? 3. Cómo se organiza el método de pr... more

Download

LAS ESTRATEGIAS

Contenidos 1. Introducción 2. ¿Qué es el método de proyectos? 3. Cómo se organiza el método de pr... more

Download

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