Papers by Luiz Sergio Keim
Brazilian Journal of Microbiology, Mar 1, 2011
In this paper we carried out a study about prevalence of the clinically significant coagulase neg... more In this paper we carried out a study about prevalence of the clinically significant coagulase negative staphylococcal (CNS) isolates found in an university hospital. Two hundred four CNS isolates from 191 patients obtained between the period of 1998 to 2002, were studied. About 27% (52/191) of the infection cases studied were confirmed as CNS-associated diseases. Blood stream infection (BSI) was the most frequent CNS associated-disease (25%; 13/52). The great majority of the BSI was verified in the Neonatal Intensive Care Unit (NICU). The analysis of the 52 patients medical history showed that 85% of the BSI was acquired in hospital. Most of the CNS nosocomial infections were associated with the use of indwelling medical devices. The incidence of methicillin-resistance among significant CNS isolates was 38%. In this study, a high percentage of exogenous contaminant was verified (60%), indicating that contamination of clinical specimens during sample collection is critical.
Arquivos brasileiros de medicina, 1995
Revista do Instituto de Medicina Tropical de São Paulo, 2019
This is an open-access article distributed under the terms of the Creative Commons Attribution Li... more This is an open-access article distributed under the terms of the Creative Commons Attribution License.
Memórias do Instituto Oswaldo Cruz, 2013
We analysed the antimicrobial susceptibility, biofilm formation and genotypic profiles of 27 isol... more We analysed the antimicrobial susceptibility, biofilm formation and genotypic profiles of 27 isolates of Staphylococcus haemolyticus obtained from the blood of 19 patients admitted to a hospital in Rio de Janeiro, Brazil. Our analysis revealed a clinical significance of 36.8% and a multi-resistance rate of 92.6% among these isolates. All but one isolate carried the mecA gene. The staphylococcal cassette chromosome mec type I was the most prevalent mec element detected (67%). Nevertheless, the isolates showed clonal diversity based on pulsed-field gel electrophoresis analysis. The ability to form biofilms was detected in 66% of the isolates studied. Surprisingly, no icaAD genes were found among the biofilm-producing isolates.
Arq Bras Med, Feb 1, 1992
Base de dados : LILACS. Pesquisa : 113067 [Identificador único]. Referências encontradas : 1 [ref... more Base de dados : LILACS. Pesquisa : 113067 [Identificador único]. Referências encontradas : 1 [refinar]. Mostrando: 1 .. 1 no formato [Detalhado]. página 1 de 1, 1 / 1, LILACS, seleciona. para imprimir. Fotocópia. Zanon, Uriel. experimental, Documentos relacionados. Id: 113067. ...
Memórias do Instituto Oswaldo Cruz, 2013
We analysed the antimicrobial susceptibility, biofilm formation and genotypic profiles of 27 isol... more We analysed the antimicrobial susceptibility, biofilm formation and genotypic profiles of 27 isolates of Staphylococcus haemolyticus obtained from the blood of 19 patients admitted to a hospital in Rio de Janeiro, Brazil. Our analysis revealed a clinical significance of 36.8% and a multi-resistance rate of 92.6% among these isolates. All but one isolate carried the mecA gene. The staphylococcal cassette chromosome mec type I was the most prevalent mec element detected (67%). Nevertheless, the isolates showed clonal diversity based on pulsed-field gel electrophoresis analysis. The ability to form biofilms was detected in 66% of the isolates studied. Surprisingly, no icaAD genes were found among the biofilm-producing isolates.
Brazilian Journal of Microbiology, 2011
In this paper we carried out a study about prevalence of the clinically significant coagulase neg... more In this paper we carried out a study about prevalence of the clinically significant coagulase negative staphylococcal (CNS) isolates found in an university hospital. Two hundred four CNS isolates from 191 patients obtained between the period of 1998 to 2002, were studied. About 27% (52/191) of the infection cases studied were confirmed as CNS-associated diseases. Blood stream infection (BSI) was the most frequent CNS associated-disease (25%; 13/52). The great majority of the BSI was verified in the Neonatal Intensive Care Unit (NICU). The analysis of the 52 patients medical history showed that 85% of the BSI was acquired in hospital. Most of the CNS nosocomial infections were associated with the use of indwelling medical devices. The incidence of methicillin-resistance among significant CNS isolates was 38%. In this study, a high percentage of exogenous contaminant was verified (60%), indicating that contamination of clinical specimens during sample collection is critical. Keim, L.S. et al. Coagulase negative Staphylococci antibiotics and the immune system (11). Although mechanisms involved in the production of biofilm are not totally clarified, bacterial products that contribute for biofilm formation are among the best-studied CNS virulence determinants, including products involved in the bacterial adhesion to solid surface and cell accumulation (2, 4, 11, 35). Many CNS also produce lipases, proteases, and other exoenzymes, which are possibly implicated with the bacterial persistence and dissemination in the host tissue (34). Although numerous species of CNS have been recognized recently, the majority is rarely pathogenic (34). Clinical studies have indicated Staphylococcus epidermidis, Staphylococcus haemolyticus, Staphylococcus warneri and Staphylococcus hominis as the most prevalent CNS in hospital infections (1, 31, 34). The emergence of antimicrobial resistant among CNS isolates is also an important factor in nosocomial infections. Furthermore, methicillin-resistant CNS (MRCNS) are crossresistant to all other ß-lactam antibiotics (28). Multiresistance has been detected in more than 80% of MRCNS hospital isolates (23). In the opposite, approximately 70% of MRCNS obtained from healthy volunteers from the community displayed higher susceptible to non-ß-lactam antibiotics (30).
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Papers by Luiz Sergio Keim