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Luis Marin

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Università degli Studi di Padova

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Università degli Studi di Padova

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Papers by Luis Marin

Circulating Tumor Cells Enumeration from the Portal Vein for Risk Stratification in Early Pancreatic Cancer Patients

Cancers

Background. Effective biomarkers are needed to enable personalized medicine for pancreatic cancer... more Background. Effective biomarkers are needed to enable personalized medicine for pancreatic cancer patients. This study analyzes the prognostic value, in early pancreatic cancer, of single circulating tumor cell (CTC) and CTC clusters from the central venous catheter (CVC) and portal blood (PV). Methods. In total, 7 mL of PV and CVC blood from 35 patients with early pancreatic cancer were analyzed. CTC were isolated using a positive immunomagnetic selection. The detection and identification of CTC were performed by immunocytochemistry (ICC) and were analyzed by Epi-fluorescence and confocal microscopy. Results. CTC and the clusters were detected both in PV and CVC. In both samples, the CTC number per cluster was higher in patients with grade three or poorly differentiated tumors (G3) than in patients with well (G1) or moderately (G2) differentiated. Patients with fewer than 185 CTC in PV exhibited a longer OS than patients with more than 185 CTC (24.5 vs. 10.0 months; p = 0.018). Sim...

Groundwater boom and bust?

Groundwater: a global assessment of scale and significance

Podria Repetirse La Catastrofe Que Hace 65 Millones De Anos Acabo Con Los Dinosaurios, Advierten Cientificos

LOS DOCTORES GERARDO SUAREZ REYNOSO, COORDINADOR DE LA INVESTIGACION CIENTIFICA, Y LUIS MARIN, IN... more LOS DOCTORES GERARDO SUAREZ REYNOSO, COORDINADOR DE LA INVESTIGACION CIENTIFICA, Y LUIS MARIN, INVESTIGADOR DEL INSTITUTO DE GEOLOGIA (IG), PARTICIPARON EN EL COLOQUIO DE INVESTIGACION SOBRE EL CRATER DE CHICXULUB Y LA MUERTE DE LOS DINOSAURIOS, EN EL MUSEO DE LAS CIENCIAS UNIVERSUM; SENALARON QUE DE NO PONER ATENCION EN EL CUIDADO DEL MEDIO AMBIENTE, LA CATASTROFE QUE ACABO CON LOS DINOSAURIOS HACE 65 MILLONES DE ANOS PUEDE REPETIRSE.

DENTAL INSURANCE CLINIC. “Asegurando su sonrisa”

Seminario Desarrollo de Emprendedores. 2014. Carrera ODONTOLOGIA. Docente Lic. Rossi Rivas M..

Clinical characterization and treatment of head and neck mucosal melanoma: a Chilean experience

Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology, 2021

OBJECTIVES Head and neck mucosal melanoma (HNMM) is a rare disease with a poor prognosis. The aim... more OBJECTIVES Head and neck mucosal melanoma (HNMM) is a rare disease with a poor prognosis. The aim of this research was to analyze clinical characteristics and prognostic factors in a Latin American cohort. STUDY DESIGN A retrospective chart review of patients with HNMM treated between 2008 and 2019 was conducted. Demographic characteristics, tumor characteristics, surgical treatment, adjuvant therapy, and oncologic outcomes were recorded. RESULTS Twenty HNMMs were identified; 60% were in men. The most frequent primary location was the nasal cavity (10; 50%). Regional disease was uncommon and limited to the oral cavity. The median overall survival was 29 months; the 3- and 5-year overall survival rates were 37.2% and 26.6%, respectively. Univariate analysis revealed that predictors of a worse overall survival were paranasal location (hazard ratio [HR], 4.61; 95% confidence interval [CI], 1.61-18.40; P = .030), positive lymph nodes (HR, 6.00; 95% CI, 1.30-27.7; P = .022), positive margins (HR, 4.32; 95% CI, 1.08-17.2; P = .039), bone invasion (HR, 3.27;95% CI, 1.05-10.1; P = .041), and lymphovascular invasion (HR, 3.82; CI, 1.03-14.2; P = .045). Three-year recurrence-free survival was 17.3%, and most of the recurrences were with distant disease. CONCLUSIONS HNMM is an infrequent disease with an aggressive behavior. Survival outcomes are related to location of the primary disease, regional spread, lymphovascular invasion, and bone invasion.

Management plan for breast cancer during the COVID‐19 pandemic. A single‐institution alternative to treat early breast cancer patients in a short time

The Breast Journal, 2020

Decrease in the prevalence of hepatitis B and D virus infections in an endemic area in Peru 23 years after the introduction of the first pilot vaccination program against hepatitis B

PLOS ONE, 2020

In 1991, Peru launched the first vaccination program against hepatitis B in children aged under 5... more In 1991, Peru launched the first vaccination program against hepatitis B in children aged under 5 years in the hyperendemic [hepatitis B virus (HBV) and hepatitis D virus (HDV)] province of Abancay. We conducted a cross-sectional study to determine the prevalence of HBV and HDV infections, 23 years after the launch of the vaccination program, as well as the post-vaccine response against hepatitis B in terms of prevalence of hepatitis B surface antibody (anti-HBs �10 mUI/ml). Among 3165 participants aged from 0 to 94 years, the prevalence rates of hepatitis B surface antigen (HBsAg), and hepatitis B core antibody (total anti-HBc) were 1.2% [95% confidence interval (CI) 0.85-1.64%], and 41.67% (95% CI 39.95-43.41%), respectively. The prevalence rate of anti-HBs at protective levels (�10 mUI/ml) in individuals who HBsAg and anti-HBc negative was 66.36% (95% CI 64.15-68.51%). The prevalence rate of HBsAg in children aged <15 years was nil, and among adult HBsAg carriers, the prevalence of hepatitis D antibody (anti-HDV) was 5.26% (2/38; 95% CI 0.64-17.74). These findings showed that HBV prevalence has changed from high to low endemicity, 23 years following implementation of the vaccination program against hepatitis B, and HDV infection was not detected in those aged <30 years.

Prevalencia de infección por los virus de la hepatitis B, D y por retrovirus en la etnia Matsés (Loreto, Peru)

Revista Peruana de Medicina Experimental y Salud Pública, 2020

Para determinar la prevalencia de infección por los virus de la hepatitis B y D (VHB y VHD, respe... more Para determinar la prevalencia de infección por los virus de la hepatitis B y D (VHB y VHD, respectivamente), VIH y HTLV-1/2 en la etnia matsés, después de la inmunización contra el VHB se realizó un estudio transversal y poblacional, utilizando pruebas de ELISA y qPCR en 963 pobladores. Las prevalencias de HBsAg, anti-HBc y anti-HBs fueron 3,3%, 36,0% y 58,7%, respectivamente. En el 3,1% de la población la carga viral fue mayor a 2000 UI/mL. En menores de 10 años, la prevalencia de HBsAg y anti-HBc fue 0,0% y 2,6%, respectivamente, mientras que en el 94,4% se encontraron anticuerpos protectores. La prevalencia de infección por el VIH y el HTLV-1/2 fue 1,5% y 0,6%, respectivamente. Se concluye que existen tasas bajas de infección por el VHB y el VHD en la población infantil de la etnia matsés. Asimismo, se confirma la presencia de infección por el VIH y el HTLV-1/2.

A Thyroid Genetic Classifier Correctly Predicts Benign Nodules with Indeterminate Cytology: Two Independent, Multicenter, Prospective Validation Trials

Thyroid, 2020

Background: Although most thyroid nodules with indeterminate cytology are benign, in most of the ... more Background: Although most thyroid nodules with indeterminate cytology are benign, in most of the world, surgery remains as the most frequent diagnostic approach. We have previously reported a 10-gene thyroid genetic classifier, which accurately predicts benign thyroid nodules. The assay is a prototype diagnostic kit suitable for reference laboratory testing and could potentially avoid unnecessary diagnostic surgery in patients with indeterminate thyroid cytology. Methods: Classifier performance was tested in two independent, ethnically diverse, prospective multicenter trials (TGCT-1/Chile and TGCT-2/USA). A total of 4061 fine-needle aspirations were collected from 15 institutions, of which 897 (22%) were called indeterminate. The clinical site was blind to the classifier score and the clinical laboratory blind to the pathology report. A matched surgical pathology and valid classifier score was available for 270 samples. Results: Cohorts showed significant differences, including (i) clinical site patient source (academic, 43% and 97% for TGCT-1 and-2, respectively); (ii) ethnic diversity, with a greater proportion of the Hispanic population (40% vs. 3%) for TGCT-1 and a greater proportion of African American (11% vs. 0%) and Asian (10% vs. 1%) populations for TGCT-2; and (iii) tumor size (mean of 1.7 and 2.5 cm for TGCT-1 and-2, respectively).

CCR6, IL7R, FAS AND MAdCAM-1 Single Nucleotide Polymorphisms are Associated With Higher Incidence of Infections in Allogeneic Stem-Cell Transplant From a Related Donor After a Reduced Intensity Conditioning Regimen: A Multicenter Experience

Clinical Lymphoma Myeloma and Leukemia, 2017

best CAR candidates for clinical development. Experimental procedures: We have established a rapi... more best CAR candidates for clinical development. Experimental procedures: We have established a rapid process for the development of functional novel CARs and demonstrate the entire workflow for one model antigen expressed on multiple myeloma cells. An initial population of scFvs against the model antigen was selected from a large naïve fully-human antibody phage display library by two rounds of panning selections. The reactivity of the polyclonal phage population was then confirmed by time-resolved fluorescence before bacterial expression of monoclonal scFvs. Selected clones from the initial scFv binding assay were converted into CAR format. We established a high-throughput flow cytometry assay simultaneously determining CAR surface expression and target antigen binding. In addition, we adapted protocols for small-scale lentiviral transduction and expansion of primary CAR T cells followed by a sensitive, high-throughput luciferase-based cytotoxicity assay. Finally, in collaboration with Wasatch Microfluidics we performed high-throughput surface plasmon resonance measurements to assess binding kinetics and cross reactivity to related antigens in the scFv-Fc format. Results: We obtained 1,323 scFvs after two rounds of antibody phage selection. The subsequent bacterial screen of 163 clones identified 23 unique monoclonal binders. CAR constructs with high expression and antigen binding were found to have affinities in the low nanomolar range and evidenced strong killing of human cancer cell lines spontaneously expressing the antigen (53-79% killing at 10:1 effector:target ratio). Importantly, in a murine xenograft model of multiple myeloma we observed specific killing of tumor cells and no overt toxicities. Conclusion: We show that our process allows the generation, screening, as well as functional and biochemical characterization of novel fully human CARs within 2 months. Our approach enables us to easily carry out comprehensive head-to-head comparisons including binding kinetics and cytotoxicity allowing the rational selection of lead CAR candidates for downstream product development.

Lower-Extremity Infections Caused by Serratia marcescens A Report of Three Cases and a Literature Review

Journal of the American Podiatric Medical Association, 2017

Serratia marcescens is a ubiquitous, facultatively anaerobic, gram-negative bacillus that has bee... more Serratia marcescens is a ubiquitous, facultatively anaerobic, gram-negative bacillus that has been cited to cause infection in immunocompromised populations. In the literature, S marcescens infections of the lower extremity have presented as granulomatous ulceration, abscess, bullous cellulitis, and necrotizing fasciitis. Herein we present a series of three cases of lower-extremity infections in which S marcescens was the sole or a contributing pathogen. We discuss the commonalities of these three cases as well as with those previously cited. All three patients presented with some combination of a similar set of clinical characteristics, including bullae formation, liquefactive necrosis, and black necrotic eschar. All three patients were diabetic and had peripheral vascular disease.

A 10-gene classifier for indeterminate thyroid nodules: Development and multicenter accuracy study

Thyroid : official journal of the American Thyroid Association, Aug 18, 2017

In most of the world, diagnostic surgery remains as the most frequent approach for indeterminate ... more In most of the world, diagnostic surgery remains as the most frequent approach for indeterminate thyroid cytology. Although several molecular tests are available for central-lab testing in the US, there are no available kits for local laboratory testing. The aim of this study was to develop a prototype in-vitro diagnostic (IVD) gene classifier for diagnosis of indeterminate thyroid cytology. In a first stage, the expression of 18 genes was determined by qPCR in a broad histopathological spectrum of fresh tissue biopsies (114). Expression data was used to train several classifiers by supervised machine learning approaches. Classifiers were tested in an independent set of 139 samples. In a second stage, the best classifier was chosen as a model to develop a multiplexed-qPCR IVD prototype assay, which was tested in a prospective multicenter cohort of fine needle aspiration (FNA) biopsies. In tissue biopsies, the best classifier, using only 10 genes, reached an optimal and consistent pe...

Nuevos medios de pago en el transporte. Proyecto BIT Madrid

Carreteras Revista Tecnica De La Asociacion Espanola De La Carretera, 2000

En este articulo se resumen algunos de los resultados de la primera fase del proyecto BIT Madrid ... more En este articulo se resumen algunos de los resultados de la primera fase del proyecto BIT Madrid (Billetica Inteligente para el Transporte de Madrid), que comprende: un analisis mundial de las experiencias existentes de implantacion de tecnologias de tarjetas sin contactos (tarjetas inteligentes), por parte de companias de transporte en el marco de una integracion tarifaria regional, y un estudio de los posibles escenarios de implantacion de esta nueva tecnologia (esquemas organizativos y financieros) en el sistema regional de transporte de Madrid y sus ventajas e inconvenientes. Se concluye afirmando que la tendencia de futuro parece clara: la convergencia hacia medios de pago electronicos y de gran flexibilidad, que permiten pagos comodos de multiples servicios con un mismo soporte.

Software for Fault Diagnosis Using Knowledge Models in Petri Nets

Dyna (Medellin, Colombia)

Near-surface seismic expression of the Chicxulub impact crater

Large meteorite impacts and planetary evolution; II, 1999

Geological Society of America Special Paper 339 1999 Near-surface seismic expression of the Chicx... more

Folding of non-Euclidean curved shells

sachusetts Amherst-Origami-based folding of 2D sheets has been of recent interest for a variety o... more sachusetts Amherst-Origami-based folding of 2D sheets has been of recent interest for a variety of applications ranging from deployable structures to self-folding robots. Though folding of planar sheets follows well-established principles, folding of curved shells involves an added level of complexity due to the inherent influence of curvature on mechanics. In this study, we use principles from differential geometry and thin shell mechanics to establish fundamental rules that govern folding of prototypical creased shells. In particular, we show how the normal curvature of a crease line controls whether the deformation is smooth or discontinuous, and investigate the influence of shell thickness and boundary conditions. We show that snap-folding of shells provides a route to rapid actuation on timescales dictated by the speed of sound. The simple geometric design principles developed can be applied at any length-scale, offering potential for bio-inspired soft actuators for tunable optics, microfluidics, and robotics.

Reverse polarity magnetized melt rocks from the cretaceous/tertiary chicxulub structure, Yucatan peninsula, Mexico

Tectonophysics, 1994

... Tectonophysics 237 (1994) 105-112 Professors Antonio Camargo, Maureen Steiner and Masaru Kono... more

A mobile robot that performs human acceptable motions

2006 IEEE/RSJ International Conference on Intelligent Robots and Systems, 2006

The presence of humans should be explicitly taken into account in all steps of robot's design and... more The presence of humans should be explicitly taken into account in all steps of robot's design and particularly for robot motion. The robot should reason about human partner's accessibility, his vision field and potential shared motions and behave as a social being by respecting social rules and protocols. This paper describes the algorithms and results of a navigation planner that takes into account the human presence explicitly. This planner is part of a human-aware motion and manipulation planning and control system that we aim to develop in order to achieve motion and manipulation tasks in presence and?or in synergy with human.

Liver recipients harbouring anti-donor preformed lymphocytotoxic antibodies exhibit a poor allograft survival at the first year after transplantation: Experience of one centre

Transplant Immunology, 2005

In this retrospective study, we analyzed the effect of the presence of anti-donor preformed alloa... more In this retrospective study, we analyzed the effect of the presence of anti-donor preformed alloantibodies in 268 liver allograft transplants. Crossmatches were performed by complement-dependent cytotoxicity (CDC) assay and HLA antibody screening by flow cytometry (FlowPRA). Positive anti-donor crossmatch was detected in 5.2% of transplants. Acute rejection frequency in +CDC crossmatch patients was not different from that observed in -CDC crossmatch patients. None of the patients transplanted with +CDC crossmatch developed chronic rejection, but they showed a significantly lower allograft survival rate, and the majority of them had allograft failures before the end of the first post-transplant year, mainly within the 3 first months. Indeed, positive FlowPRA determination was concordant with data from the CDC assay. In conclusion, these findings show a direct correlation between the presence of anti-donor preformed antibodies and a poor allograft survival in liver transplant.

Fuzzy arithmetic and fuzzy ranking: a nonstandard approach

Apparatus is disclosed for providing a modulo-n check character as a function of a sequence of da... more Apparatus is disclosed for providing a modulo-n check character as a function of a sequence of data characters. The check character may be used to permit a check of the data character sequence for its correctness, for example, after being transmitted to a data processing installation or after printing. In the preferred embodiments each data character of the sequence is generated by one of a plurality of data character carriers which are selectively set to generate a given character. An additional check carrier is provided to generate the check character. The check carrier is set by a predetermined amount each time one of the data carriers is set so that the ultimately generated check character is a function of the data character sequence.

Circulating Tumor Cells Enumeration from the Portal Vein for Risk Stratification in Early Pancreatic Cancer Patients

Cancers

Background. Effective biomarkers are needed to enable personalized medicine for pancreatic cancer... more Background. Effective biomarkers are needed to enable personalized medicine for pancreatic cancer patients. This study analyzes the prognostic value, in early pancreatic cancer, of single circulating tumor cell (CTC) and CTC clusters from the central venous catheter (CVC) and portal blood (PV). Methods. In total, 7 mL of PV and CVC blood from 35 patients with early pancreatic cancer were analyzed. CTC were isolated using a positive immunomagnetic selection. The detection and identification of CTC were performed by immunocytochemistry (ICC) and were analyzed by Epi-fluorescence and confocal microscopy. Results. CTC and the clusters were detected both in PV and CVC. In both samples, the CTC number per cluster was higher in patients with grade three or poorly differentiated tumors (G3) than in patients with well (G1) or moderately (G2) differentiated. Patients with fewer than 185 CTC in PV exhibited a longer OS than patients with more than 185 CTC (24.5 vs. 10.0 months; p = 0.018). Sim...

Groundwater boom and bust?

Groundwater: a global assessment of scale and significance

Podria Repetirse La Catastrofe Que Hace 65 Millones De Anos Acabo Con Los Dinosaurios, Advierten Cientificos

LOS DOCTORES GERARDO SUAREZ REYNOSO, COORDINADOR DE LA INVESTIGACION CIENTIFICA, Y LUIS MARIN, IN... more LOS DOCTORES GERARDO SUAREZ REYNOSO, COORDINADOR DE LA INVESTIGACION CIENTIFICA, Y LUIS MARIN, INVESTIGADOR DEL INSTITUTO DE GEOLOGIA (IG), PARTICIPARON EN EL COLOQUIO DE INVESTIGACION SOBRE EL CRATER DE CHICXULUB Y LA MUERTE DE LOS DINOSAURIOS, EN EL MUSEO DE LAS CIENCIAS UNIVERSUM; SENALARON QUE DE NO PONER ATENCION EN EL CUIDADO DEL MEDIO AMBIENTE, LA CATASTROFE QUE ACABO CON LOS DINOSAURIOS HACE 65 MILLONES DE ANOS PUEDE REPETIRSE.

DENTAL INSURANCE CLINIC. “Asegurando su sonrisa”

Seminario Desarrollo de Emprendedores. 2014. Carrera ODONTOLOGIA. Docente Lic. Rossi Rivas M..

Clinical characterization and treatment of head and neck mucosal melanoma: a Chilean experience

Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology, 2021

OBJECTIVES Head and neck mucosal melanoma (HNMM) is a rare disease with a poor prognosis. The aim... more OBJECTIVES Head and neck mucosal melanoma (HNMM) is a rare disease with a poor prognosis. The aim of this research was to analyze clinical characteristics and prognostic factors in a Latin American cohort. STUDY DESIGN A retrospective chart review of patients with HNMM treated between 2008 and 2019 was conducted. Demographic characteristics, tumor characteristics, surgical treatment, adjuvant therapy, and oncologic outcomes were recorded. RESULTS Twenty HNMMs were identified; 60% were in men. The most frequent primary location was the nasal cavity (10; 50%). Regional disease was uncommon and limited to the oral cavity. The median overall survival was 29 months; the 3- and 5-year overall survival rates were 37.2% and 26.6%, respectively. Univariate analysis revealed that predictors of a worse overall survival were paranasal location (hazard ratio [HR], 4.61; 95% confidence interval [CI], 1.61-18.40; P = .030), positive lymph nodes (HR, 6.00; 95% CI, 1.30-27.7; P = .022), positive margins (HR, 4.32; 95% CI, 1.08-17.2; P = .039), bone invasion (HR, 3.27;95% CI, 1.05-10.1; P = .041), and lymphovascular invasion (HR, 3.82; CI, 1.03-14.2; P = .045). Three-year recurrence-free survival was 17.3%, and most of the recurrences were with distant disease. CONCLUSIONS HNMM is an infrequent disease with an aggressive behavior. Survival outcomes are related to location of the primary disease, regional spread, lymphovascular invasion, and bone invasion.

Management plan for breast cancer during the COVID‐19 pandemic. A single‐institution alternative to treat early breast cancer patients in a short time

The Breast Journal, 2020

Decrease in the prevalence of hepatitis B and D virus infections in an endemic area in Peru 23 years after the introduction of the first pilot vaccination program against hepatitis B

PLOS ONE, 2020

In 1991, Peru launched the first vaccination program against hepatitis B in children aged under 5... more In 1991, Peru launched the first vaccination program against hepatitis B in children aged under 5 years in the hyperendemic [hepatitis B virus (HBV) and hepatitis D virus (HDV)] province of Abancay. We conducted a cross-sectional study to determine the prevalence of HBV and HDV infections, 23 years after the launch of the vaccination program, as well as the post-vaccine response against hepatitis B in terms of prevalence of hepatitis B surface antibody (anti-HBs �10 mUI/ml). Among 3165 participants aged from 0 to 94 years, the prevalence rates of hepatitis B surface antigen (HBsAg), and hepatitis B core antibody (total anti-HBc) were 1.2% [95% confidence interval (CI) 0.85-1.64%], and 41.67% (95% CI 39.95-43.41%), respectively. The prevalence rate of anti-HBs at protective levels (�10 mUI/ml) in individuals who HBsAg and anti-HBc negative was 66.36% (95% CI 64.15-68.51%). The prevalence rate of HBsAg in children aged <15 years was nil, and among adult HBsAg carriers, the prevalence of hepatitis D antibody (anti-HDV) was 5.26% (2/38; 95% CI 0.64-17.74). These findings showed that HBV prevalence has changed from high to low endemicity, 23 years following implementation of the vaccination program against hepatitis B, and HDV infection was not detected in those aged <30 years.

Prevalencia de infección por los virus de la hepatitis B, D y por retrovirus en la etnia Matsés (Loreto, Peru)

Revista Peruana de Medicina Experimental y Salud Pública, 2020

Para determinar la prevalencia de infección por los virus de la hepatitis B y D (VHB y VHD, respe... more Para determinar la prevalencia de infección por los virus de la hepatitis B y D (VHB y VHD, respectivamente), VIH y HTLV-1/2 en la etnia matsés, después de la inmunización contra el VHB se realizó un estudio transversal y poblacional, utilizando pruebas de ELISA y qPCR en 963 pobladores. Las prevalencias de HBsAg, anti-HBc y anti-HBs fueron 3,3%, 36,0% y 58,7%, respectivamente. En el 3,1% de la población la carga viral fue mayor a 2000 UI/mL. En menores de 10 años, la prevalencia de HBsAg y anti-HBc fue 0,0% y 2,6%, respectivamente, mientras que en el 94,4% se encontraron anticuerpos protectores. La prevalencia de infección por el VIH y el HTLV-1/2 fue 1,5% y 0,6%, respectivamente. Se concluye que existen tasas bajas de infección por el VHB y el VHD en la población infantil de la etnia matsés. Asimismo, se confirma la presencia de infección por el VIH y el HTLV-1/2.

A Thyroid Genetic Classifier Correctly Predicts Benign Nodules with Indeterminate Cytology: Two Independent, Multicenter, Prospective Validation Trials

Thyroid, 2020

Background: Although most thyroid nodules with indeterminate cytology are benign, in most of the ... more Background: Although most thyroid nodules with indeterminate cytology are benign, in most of the world, surgery remains as the most frequent diagnostic approach. We have previously reported a 10-gene thyroid genetic classifier, which accurately predicts benign thyroid nodules. The assay is a prototype diagnostic kit suitable for reference laboratory testing and could potentially avoid unnecessary diagnostic surgery in patients with indeterminate thyroid cytology. Methods: Classifier performance was tested in two independent, ethnically diverse, prospective multicenter trials (TGCT-1/Chile and TGCT-2/USA). A total of 4061 fine-needle aspirations were collected from 15 institutions, of which 897 (22%) were called indeterminate. The clinical site was blind to the classifier score and the clinical laboratory blind to the pathology report. A matched surgical pathology and valid classifier score was available for 270 samples. Results: Cohorts showed significant differences, including (i) clinical site patient source (academic, 43% and 97% for TGCT-1 and-2, respectively); (ii) ethnic diversity, with a greater proportion of the Hispanic population (40% vs. 3%) for TGCT-1 and a greater proportion of African American (11% vs. 0%) and Asian (10% vs. 1%) populations for TGCT-2; and (iii) tumor size (mean of 1.7 and 2.5 cm for TGCT-1 and-2, respectively).

CCR6, IL7R, FAS AND MAdCAM-1 Single Nucleotide Polymorphisms are Associated With Higher Incidence of Infections in Allogeneic Stem-Cell Transplant From a Related Donor After a Reduced Intensity Conditioning Regimen: A Multicenter Experience

Clinical Lymphoma Myeloma and Leukemia, 2017

best CAR candidates for clinical development. Experimental procedures: We have established a rapi... more best CAR candidates for clinical development. Experimental procedures: We have established a rapid process for the development of functional novel CARs and demonstrate the entire workflow for one model antigen expressed on multiple myeloma cells. An initial population of scFvs against the model antigen was selected from a large naïve fully-human antibody phage display library by two rounds of panning selections. The reactivity of the polyclonal phage population was then confirmed by time-resolved fluorescence before bacterial expression of monoclonal scFvs. Selected clones from the initial scFv binding assay were converted into CAR format. We established a high-throughput flow cytometry assay simultaneously determining CAR surface expression and target antigen binding. In addition, we adapted protocols for small-scale lentiviral transduction and expansion of primary CAR T cells followed by a sensitive, high-throughput luciferase-based cytotoxicity assay. Finally, in collaboration with Wasatch Microfluidics we performed high-throughput surface plasmon resonance measurements to assess binding kinetics and cross reactivity to related antigens in the scFv-Fc format. Results: We obtained 1,323 scFvs after two rounds of antibody phage selection. The subsequent bacterial screen of 163 clones identified 23 unique monoclonal binders. CAR constructs with high expression and antigen binding were found to have affinities in the low nanomolar range and evidenced strong killing of human cancer cell lines spontaneously expressing the antigen (53-79% killing at 10:1 effector:target ratio). Importantly, in a murine xenograft model of multiple myeloma we observed specific killing of tumor cells and no overt toxicities. Conclusion: We show that our process allows the generation, screening, as well as functional and biochemical characterization of novel fully human CARs within 2 months. Our approach enables us to easily carry out comprehensive head-to-head comparisons including binding kinetics and cytotoxicity allowing the rational selection of lead CAR candidates for downstream product development.

Lower-Extremity Infections Caused by Serratia marcescens A Report of Three Cases and a Literature Review

Journal of the American Podiatric Medical Association, 2017

Serratia marcescens is a ubiquitous, facultatively anaerobic, gram-negative bacillus that has bee... more Serratia marcescens is a ubiquitous, facultatively anaerobic, gram-negative bacillus that has been cited to cause infection in immunocompromised populations. In the literature, S marcescens infections of the lower extremity have presented as granulomatous ulceration, abscess, bullous cellulitis, and necrotizing fasciitis. Herein we present a series of three cases of lower-extremity infections in which S marcescens was the sole or a contributing pathogen. We discuss the commonalities of these three cases as well as with those previously cited. All three patients presented with some combination of a similar set of clinical characteristics, including bullae formation, liquefactive necrosis, and black necrotic eschar. All three patients were diabetic and had peripheral vascular disease.

A 10-gene classifier for indeterminate thyroid nodules: Development and multicenter accuracy study

Thyroid : official journal of the American Thyroid Association, Aug 18, 2017

In most of the world, diagnostic surgery remains as the most frequent approach for indeterminate ... more In most of the world, diagnostic surgery remains as the most frequent approach for indeterminate thyroid cytology. Although several molecular tests are available for central-lab testing in the US, there are no available kits for local laboratory testing. The aim of this study was to develop a prototype in-vitro diagnostic (IVD) gene classifier for diagnosis of indeterminate thyroid cytology. In a first stage, the expression of 18 genes was determined by qPCR in a broad histopathological spectrum of fresh tissue biopsies (114). Expression data was used to train several classifiers by supervised machine learning approaches. Classifiers were tested in an independent set of 139 samples. In a second stage, the best classifier was chosen as a model to develop a multiplexed-qPCR IVD prototype assay, which was tested in a prospective multicenter cohort of fine needle aspiration (FNA) biopsies. In tissue biopsies, the best classifier, using only 10 genes, reached an optimal and consistent pe...

Nuevos medios de pago en el transporte. Proyecto BIT Madrid

Carreteras Revista Tecnica De La Asociacion Espanola De La Carretera, 2000

En este articulo se resumen algunos de los resultados de la primera fase del proyecto BIT Madrid ... more En este articulo se resumen algunos de los resultados de la primera fase del proyecto BIT Madrid (Billetica Inteligente para el Transporte de Madrid), que comprende: un analisis mundial de las experiencias existentes de implantacion de tecnologias de tarjetas sin contactos (tarjetas inteligentes), por parte de companias de transporte en el marco de una integracion tarifaria regional, y un estudio de los posibles escenarios de implantacion de esta nueva tecnologia (esquemas organizativos y financieros) en el sistema regional de transporte de Madrid y sus ventajas e inconvenientes. Se concluye afirmando que la tendencia de futuro parece clara: la convergencia hacia medios de pago electronicos y de gran flexibilidad, que permiten pagos comodos de multiples servicios con un mismo soporte.

Software for Fault Diagnosis Using Knowledge Models in Petri Nets

Dyna (Medellin, Colombia)

Near-surface seismic expression of the Chicxulub impact crater

Large meteorite impacts and planetary evolution; II, 1999

Geological Society of America Special Paper 339 1999 Near-surface seismic expression of the Chicx... more

Folding of non-Euclidean curved shells

sachusetts Amherst-Origami-based folding of 2D sheets has been of recent interest for a variety o... more sachusetts Amherst-Origami-based folding of 2D sheets has been of recent interest for a variety of applications ranging from deployable structures to self-folding robots. Though folding of planar sheets follows well-established principles, folding of curved shells involves an added level of complexity due to the inherent influence of curvature on mechanics. In this study, we use principles from differential geometry and thin shell mechanics to establish fundamental rules that govern folding of prototypical creased shells. In particular, we show how the normal curvature of a crease line controls whether the deformation is smooth or discontinuous, and investigate the influence of shell thickness and boundary conditions. We show that snap-folding of shells provides a route to rapid actuation on timescales dictated by the speed of sound. The simple geometric design principles developed can be applied at any length-scale, offering potential for bio-inspired soft actuators for tunable optics, microfluidics, and robotics.

Reverse polarity magnetized melt rocks from the cretaceous/tertiary chicxulub structure, Yucatan peninsula, Mexico

Tectonophysics, 1994

... Tectonophysics 237 (1994) 105-112 Professors Antonio Camargo, Maureen Steiner and Masaru Kono... more

A mobile robot that performs human acceptable motions

2006 IEEE/RSJ International Conference on Intelligent Robots and Systems, 2006

The presence of humans should be explicitly taken into account in all steps of robot's design and... more The presence of humans should be explicitly taken into account in all steps of robot's design and particularly for robot motion. The robot should reason about human partner's accessibility, his vision field and potential shared motions and behave as a social being by respecting social rules and protocols. This paper describes the algorithms and results of a navigation planner that takes into account the human presence explicitly. This planner is part of a human-aware motion and manipulation planning and control system that we aim to develop in order to achieve motion and manipulation tasks in presence and?or in synergy with human.

Liver recipients harbouring anti-donor preformed lymphocytotoxic antibodies exhibit a poor allograft survival at the first year after transplantation: Experience of one centre

Transplant Immunology, 2005

In this retrospective study, we analyzed the effect of the presence of anti-donor preformed alloa... more In this retrospective study, we analyzed the effect of the presence of anti-donor preformed alloantibodies in 268 liver allograft transplants. Crossmatches were performed by complement-dependent cytotoxicity (CDC) assay and HLA antibody screening by flow cytometry (FlowPRA). Positive anti-donor crossmatch was detected in 5.2% of transplants. Acute rejection frequency in +CDC crossmatch patients was not different from that observed in -CDC crossmatch patients. None of the patients transplanted with +CDC crossmatch developed chronic rejection, but they showed a significantly lower allograft survival rate, and the majority of them had allograft failures before the end of the first post-transplant year, mainly within the 3 first months. Indeed, positive FlowPRA determination was concordant with data from the CDC assay. In conclusion, these findings show a direct correlation between the presence of anti-donor preformed antibodies and a poor allograft survival in liver transplant.

Fuzzy arithmetic and fuzzy ranking: a nonstandard approach

Apparatus is disclosed for providing a modulo-n check character as a function of a sequence of da... more Apparatus is disclosed for providing a modulo-n check character as a function of a sequence of data characters. The check character may be used to permit a check of the data character sequence for its correctness, for example, after being transmitted to a data processing installation or after printing. In the preferred embodiments each data character of the sequence is generated by one of a plurality of data character carriers which are selectively set to generate a given character. An additional check carrier is provided to generate the check character. The check carrier is set by a predetermined amount each time one of the data carriers is set so that the ultimately generated check character is a function of the data character sequence.