Papers by Konrad Weroński
The NS3 (513-522) fragment of the hepatitis G virus (HGV) was manually synthesised by solid phase... more The NS3 (513-522) fragment of the hepatitis G virus (HGV) was manually synthesised by solid phase synthesis following Fluoren-9ylmethoxycarbonyl (Fmoc)/tBut strategy. It was characterised by amino acid analysis, analytical HPLC and FAB-mass spectrometry. It showed strong hydrophilic properties as predicted by applying the Hopp and Woods scale. In an effort to reduce these properties to improve its affinity for lipidic membrane models as lipid monolayers and liposomes, the palmitoyl derivative of the peptide was synthesised. The lipophilic derivative was obtained by following the same strategy stated above and final acylation with palmitic acid. Surface activity measurements show that palmitoyl-NS3 (513-522), in contrast with the parent peptide, is able to modify the surface activity of water-forming monomolecular films. The result of the study indicates that saturation of superficial pressure is gained proportionally to the increased amounts of injected palmitoyl-NS3 (513-522).
Colloids and Surfaces B: Biointerfaces, 2007
Two decapeptide fragments of the non-structural hepatitis G NS3 protein (GBV-C/HGV), 513-522 (RGR... more Two decapeptide fragments of the non-structural hepatitis G NS3 protein (GBV-C/HGV), 513-522 (RGRTGRGRSG) and 505-514 (SAELSMQRRG), as well as their palmitoylated derivatives were synthesized. The physico-chemical properties of the peptides were analyzed in both the absence and presence of the zwitterionic 1,2-dipalmitoyl-sn-glycero-3-phosphatidylcholine (DPPC), the negative 1,2-dipalmitoyl-snglycero-3-[phospho-rac-(1-glycerol)] (DPPG) and the positive 1,2-dioeloyl-3-trimethylammonium-propane (DOTAP) lipid monolayers. Based on their high hydrophilic properties, neither parent peptide presented surface activity and their incorporation into lipid monolayers was low. In contrast, their palmitoylated derivatives showed concentration-dependent surface activity and could be inserted into lipid monolayers to varying degrees depending on their sequence. Compression isotherms showed that the presence of palmitoylated peptides in the subphase resulted in a molecular arrangement less condensed than that corresponding to the pure phospholipid. In concordance with the monolayer results, differential scanning calorimetry (DSC) demonstrated that the parent peptides did not have any effect on the thermograms, while the palmitoylated derivatives affected the thermotropic properties of DPPC bilayers.
Uploads
Papers by Konrad Weroński