Papers by Kerem şenvardar
We propose a computing method for the estimation of real-time PCR ampli®cation ef®ciency. It is b... more We propose a computing method for the estimation of real-time PCR ampli®cation ef®ciency. It is based on a statistic delimitation of the beginning of exponentially behaving observations in real-time PCR kinetics. PCR ground¯uorescence phase, nonexponential and plateau phase were excluded from the calculation process by separate mathematical algorithms. We validated the method on experimental data on multiple targets obtained on the LightCycler platform. The developed method yields results of higher accuracy than the currently used method of serial dilutions for ampli®cation ef®ciency estimation. The single reaction set-up estimation is sensitive to differences in starting concentrations of the target sequence in samples. Furthermore, it resists the subjective in¯uence of researchers, and the estimation can therefore be fully instrumentalized.
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Papers by Kerem şenvardar