Kazumi Norose

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Prospere Remy

Amlan Gupta

Sikkim Manipal University

James Yahaya

Martin Johannes Enk

Instituto Evandro Chagas / Evandro Chagas Institute

Iftikhar Jan

National Institute of Child Health

Ipseet Mishra

Orhan Ozkan

Sze Li Siow

University Malaysia Sarawak

lucieni conterno

Faculdade de Medicina de Marília

eduardo monge

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Papers by Kazumi Norose

Ascaris lumbricoides causing acute abdomen: a case report

Eastern Mediterranean Health Journal, 2013

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Enhanced cytotoxicity of IFN-gamma-producing CD4+ cytotoxic T lymphocytes specific for T. gondii-infected human melanoma cells

Journal of Immunology, 1995

CD4+ lines specific for Toxoplasma gondii-infected human melanoma P36 cells were established from... more CD4+ lines specific for Toxoplasma gondii-infected human melanoma P36 cells were established from PBL of a patient with chronic toxoplasmosis. CD4+ CTL lines were obtained by weekly in vitro stimulation with T. gondii-infected P36 cells that shared HLA-DR4 molecules with the patient. The lytic activity of CD4+ CTL lines against T. gondii-infected P36 or T. gondii-infected autologous EBV-transformed B lymphoma (EBV-Ya) was inhibited by anti-HLA-DR mAb, whereas anti-HLA-A, B, C mAb failed to block the lytic activity. Thus, the cytotoxicity of CD4+ CTL lines against T. gondii-infected P36 was restricted by HLA-DR molecules. In response to Ag-specific stimulation, CD4+ CTL lines produced significant levels of IFN-gamma. Exogenously added IFN-gamma up-regulated the surface expression of MHC class II, but not of class I in T. gondii-infected P36 cells. In addition, the CTL activity against T. gondii-infected P36 cells was augmented when target cells were co-cultured with IFN-gamma. These ...

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The role of IFN-gamma and Toll-like receptors in nephropathy induced by Toxoplasma gondii infection

Microbiology and immunology, 2004

The pathologic links between Toxoplasma gondii infections and renal diseases have not yet been es... more The pathologic links between Toxoplasma gondii infections and renal diseases have not yet been established. Gamma interferon (IFN-gamma) and Toll-like receptors (TLRs) are involved in the host defense mechanism against T. gondii infection. The role of IFN-gamma and TLRs in renal function of T. gondii -infected mice was studied using wild type (WT), TLR2-deficient and TLR4-deficient mice perorally infected with cysts of an avirulent cyst-forming Fukaya strain of T. gondii. T. gondii was abundant in kidneys in IFN-gamma KO (GKO) mice as determined by a quantitative competitive-polymerase chain reaction (QC-PCR). But, T. gondii was not detected in kidneys in WT, TLR2-deficient and TLR4-deficient mice. Interestingly, renal function of TLR2-deficient and TLR4-deficient mice was damaged as evaluated by serum creatinine, serum blood urea nitrogen (BUN), and urine albumin/creatinine ratio (ACR), whereas renal function of GKO and WT mice was not damaged. Histopathology of TLR2-deficient mice...

Dominance of activated T cells and interleukin-6 in aqueous humor in Vogt-Koyanagi-Harada disease

Investigative ophthalmology & visual science, 1994

PURPOSE To determine the immunopathologic role of the lymphocytes and lymphokines in aqueous humo... more PURPOSE To determine the immunopathologic role of the lymphocytes and lymphokines in aqueous humor (AH) of patients with Vogt-Koyanagi-Harada disease (VKH). METHODS The distribution of leukocyte subsets in the peripheral blood and AH was examined using fluorescein isothiocyanate-conjugated monoclonal antibodies. The levels of lymphokines, such as interleukin-2 (IL-2) and interleukin-6 (IL-6), in the sera, AH, and cerebrospinal fluid from the patients with VKH were determined using an enzyme-linked immunosorbent assay. RESULTS T cells constituted the majority of lymphocytes within AH. The value for CD4+ cells (helper/inducer T lymphocytes) in AH was 51.7% +/- 14.9% (mean +/- SD) and that for CD8+ cells (cytotoxic/suppressor T lymphocytes) was 31.1% +/- 13.0%. The percentage of HLA-DR+ cells (B lymphocytes, monocytes, macrophages, and activated T lymphocytes) in AH (50.8% +/- 24.9%) significantly exceeded (P < 0.001) that in blood (13.1% +/- 4.2%). The percentage of CD8+ cells in A...

SPARC deficiency leads to early-onset cataractogenesis

Investigative ophthalmology & visual science, 1998

PURPOSE To determine the role of SPARC (secreted protein, acidic, and rich in cysteine) in catara... more PURPOSE To determine the role of SPARC (secreted protein, acidic, and rich in cysteine) in cataractogenesis by examining mice deficient in a matricellular protein SPARC. METHODS Mice were rendered SPARC-deficient by a targeted disruption of the gene. Slit-lamp microscopy and histology were used to examine the eyes of SPARC-null and wild-type mice from birth to 14 months of age. RESULTS SPARC-null mice developed opacities in the posterior cortex of the eye as early as 1.5 months after birth. The diffuse cataracts appeared to progress toward the anterior cortex and reached maturity in many animals by 3.5 months of age. Early stages of cataractogenesis in SPARC-null mice included inhibition of normal lens fiber cell differentiation, degeneration of fiber cells, vacuole formation at the equator, and liquefaction of the cortex. No cataracts were detected in wild-type mice up to the age of 8 months. CONCLUSIONS The early onset of cataracts in SPARC-null mice establishes that the gene is e...

[Toxoplasmosis-associated central nervous system vasculitis accompanied by multiple cerebral hemorrhages developing subsequent to cord blood transplantation]

[Rinsho ketsueki] The Japanese journal of clinical hematology, 2019

A 57-year-old man with high-risk myelodysplastic syndrome underwent umbilical cord blood transpla... more A 57-year-old man with high-risk myelodysplastic syndrome underwent umbilical cord blood transplantation. He began receiving steroids on day 14 for acute graft-versus-host disease, and experienced dizziness on day 75 during gradual dose reduction. Multiple hemorrhages were observed in the cerebrum, cerebellum, and brainstem. His bleeding increased, and he underwent a brain biopsy on day 91. Subsequently, he was diagnosed with central nervous system vasculitis (CNSV) on the basis of the observed aggregation of mature CD3+ lymphocytes around small vessels and vascular wall invasion by lymphocytes and macrophages. After receiving high-dose steroid therapy, cerebral hemorrhage stopped; however, dysphasia occurred on day 113 and the patient died of cerebral edema on day 128. Toxoplasma DNA and tachyzoites were detected in the brain biopsy specimen during additional examinations; therefore, we suspected that the toxoplasmosis was related to the onset of CNSV. CNSV is a rare, rapidly progr...

[Electrically evoked response (EER) of the visual system--effect of light adaptation]

Nippon Ganka Gakkai zasshi, 1987

Ocular findings in a patient with Prader-Willi syndrome

Japanese journal of ophthalmology, 1995

A 25-year-old woman is described whose clinical features included infantile hypotonia, obesity af... more A 25-year-old woman is described whose clinical features included infantile hypotonia, obesity after infancy, intellectual impairment, dysmorphic facial features, short stature, small hands and feet, and abnormal dentition, which are typical of the Prader-Willi syndrome. The patient had almond-shaped eyes, spot-like hypopigmentation under the retina, and a polychromatic luster in the anterior and posterior subcapsular regions of both lenses. Using fluorescence fundus angiography, we identified choroid-transmitted fluorescence in the areas of spot-like hypopigmentation. Recordings of both electroretinogram and visual evoked potential were normal. Ophthalmologists should be aware of the characteristic features of Prader-Willi syndrome because some of the ocular disorders associated with this syndrome can be treated.

Toxoplasma Infection Induces Sustained Up-Regulation of Complement Factor B and C5a Receptor in the Mouse Brain via Microglial Activation: Implication for the Alternative Complement Pathway Activation and Anaphylatoxin Signaling in Cerebral Toxoplasmosis

Frontiers in Immunology

Toxoplasma gondii is a neurotropic protozoan parasite, which is linked to neurological manifestat... more Toxoplasma gondii is a neurotropic protozoan parasite, which is linked to neurological manifestations in immunocompromised individuals as well as severe neurodevelopmental sequelae in congenital toxoplasmosis. While the complement system is the first line of host defense that plays a significant role in the prevention of parasite dissemination, Toxoplasma artfully evades complement-mediated clearance via recruiting complement regulatory proteins to their surface. On the other hand, the details of Toxoplasma and the complement system interaction in the brain parenchyma remain elusive. In this study, infection-induced changes in the mRNA levels of complement components were analyzed by quantitative PCR using a murine Toxoplasma infection model in vivo and primary glial cells in vitro. In addition to the core components C3 and C1q, anaphylatoxin C3a and C5a receptors (C3aR and C5aR1), as well as alternative complement pathway components properdin (CFP) and factor B (CFB), were signific...

Immunologic analysis of cerebrospinal fluid lymphocytes in Vogt-Koyanagi-Harada disease

In this study, we focused upon the immunologic aspects of Vogt-Koyanagi-Harada disease (VKH) by c... more In this study, we focused upon the immunologic aspects of Vogt-Koyanagi-Harada disease (VKH) by comparing the cytotoxic activity of peripheral blood leukocytes (PBL) to that of cerebrospinal fluid leukocytes (CSFL) against the human melanoma cell line (P-36) and the human cervical carcinoma cell line (HeLa-S3). The PBL from patients with VKH showed significant cytotoxic activity against P-36 (P less than 0.01), but did not show cytotoxic activity against HeLa-S3. The CSFL showed significantly weaker cytotoxic activity against P-36 compared to that of PBL (P less than 0.02). We also analyzed the cell membrane surface markers applying monoclonal antibodies on PBL and CSFL. The percentage of OKT8+ (CD8: T cytotoxic/suppressor lymphocytes) cells was significantly lower in CSFL than in PBL (P less than 0.05). There was a tendency toward a higher percentage of HLA-DR+ cells (B lymphocytes, monocytes, macrophages, and activated T lymphocytes) and a higher ratio of OKT4+/8+ cells (CD4/CD8: ...

Comparative Study of Interferon-Gamma Production by Toxoplasma Gondii-Infected B Lymphoma Cell-Specific CD8+ CTL and Toxoplasma Gondii-Infected Melan Oma Cell-Specific CD4+ CTL

[Analysis of infiltrating lymphocytes in choroidal melanoma]

We performed immunohistochemistry on tumor infiltrating lymphocytes (TILs) in a 78-year-old man w... more We performed immunohistochemistry on tumor infiltrating lymphocytes (TILs) in a 78-year-old man with choroidal malignant melanoma. Cell suspensions of TILs from fresh specimens and peripheral blood lymphocytes (PBLs) were stained with anti-CD3, anti-CD4, anti-CD8, anti-CD29, anti-CD45RA, and anti-human leukocyte antigen (HLA)-DR monoclonal antibodies and analyzed using three-color flow cytometry. In light microscopy, the number of infiltrating lymphocytes around the tumor was very small. Immunohistochemically, T lymphocytes were more numerous than B lymphocytes. Flow cytometric analysis showed that CD8+ cells were more numerous than CD4+ cells in CD3+ cells in TILs, and most of these cells also expressed HLA-DR antigen. CD29+ (memory) cells were increased and CD45RA+ (naive) cells were decreased in CD4+ cells in TILs as compared with PBLs. We concluded that the increase in the percentage of activated memory T lymphocytes and the decrease of naive T lymphocytes may reflect a localize...

Disseminated toxoplasmosis with atypical symptoms which developed with exacerbation of systemic lupus erythematosus

Lupus

Toxoplasma is a common parasite worldwide that mainly affects the brain, lungs and eyes. Although... more Toxoplasma is a common parasite worldwide that mainly affects the brain, lungs and eyes. Although toxoplasmic encephalitis is a lethal disease without treatment, past case reports show most patients with systemic lupus erythematosus who developed toxoplasmic encephalitis were misdiagnosed and treated as neuropsychiatric systemic lupus erythematosus, which led to unfavorable outcomes. We herein describe a case of disseminated toxoplasmosis affecting all the above organs with atypical symptoms, which developed with exacerbation of systemic lupus erythematosus. She had initially manifested with retinochoroiditis without vitritis, mild cognitive impairment and an isolated lung mass. These are completely different from the classic symptoms of toxoplasmosis that have been reported in patients with HIV infection and/or those after hematopoietic transplantation. Our case, together with previously reported cases, suggests the manifestation of toxoplasmosis that develops in systemic lupus ery...

Enhanced cytotoxicity of IFN-gamma-producing CD4+ cytotoxic T lymphocytes specific for T. gondii-infected human melanoma cells

The Journal of …, 1995

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Short Communications Polymerase Chain Reaction: A Better Method for Diagnosing Chronic Schistosoma mansoni Infections

Abstract: For more effective diagnosis of the acute and chronic stages of Schistosoma mansoni in... more Abstract: For more effective diagnosis of the acute and chronic stages of Schistosoma mansoni infection in humans, the polymerase chain reaction (PCR) technique was compared with the Kato-Katz method. A total of 150 stool samples were collected from inpatient and outpatient clinics at the Department of Tropical Medicine, Minia

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Toxoplasmic Encephalitis Followed by Primary EBV-Associated Post-Transplant Lymphoproliferative Disorder of the Central Nervous System in a Patient Undergoing Allogeneic Hematopoietic Stem Cell Transplant: A Case Report

Transplantation Proceedings

P55 Roles of Toxoplasma gondii HSP70 and T. gondii HSP30/bag1 in protective immunity of T. gondii-infected mice

Medical Entomology and Zoology

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P18 In vitro stage conversion of Toxoplasma gondii

Medical Entomology and Zoology

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P69 Analysis of genetically controlled organ-specific infectivity of Toxoplasma gondii by using IFN-γ KO mice and parabiosed rat

Medical Entomology and Zoology

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Desensitization to trimethoprim-sulfamethoxazole in a toxoplasmic encephalitis patient who was intolerant to conventional treatments

Journal of Infection and Chemotherapy

Ascaris lumbricoides causing acute abdomen: a case report

Eastern Mediterranean Health Journal, 2013

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Enhanced cytotoxicity of IFN-gamma-producing CD4+ cytotoxic T lymphocytes specific for T. gondii-infected human melanoma cells

Journal of Immunology, 1995

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The role of IFN-gamma and Toll-like receptors in nephropathy induced by Toxoplasma gondii infection

Microbiology and immunology, 2004

Dominance of activated T cells and interleukin-6 in aqueous humor in Vogt-Koyanagi-Harada disease

Investigative ophthalmology & visual science, 1994

SPARC deficiency leads to early-onset cataractogenesis

Investigative ophthalmology & visual science, 1998

[Toxoplasmosis-associated central nervous system vasculitis accompanied by multiple cerebral hemorrhages developing subsequent to cord blood transplantation]

[Rinsho ketsueki] The Japanese journal of clinical hematology, 2019

[Electrically evoked response (EER) of the visual system--effect of light adaptation]

Nippon Ganka Gakkai zasshi, 1987

Ocular findings in a patient with Prader-Willi syndrome

Japanese journal of ophthalmology, 1995

Frontiers in Immunology

Immunologic analysis of cerebrospinal fluid lymphocytes in Vogt-Koyanagi-Harada disease

Comparative Study of Interferon-Gamma Production by Toxoplasma Gondii-Infected B Lymphoma Cell-Specific CD8+ CTL and Toxoplasma Gondii-Infected Melan Oma Cell-Specific CD4+ CTL

[Analysis of infiltrating lymphocytes in choroidal melanoma]