Bu çalışmada ebeveyn olarak RP40xQ, HD324, PM702, Kandil, PM217, HDA337, HDH23 ve VU33 biber çeşi... more Bu çalışmada ebeveyn olarak RP40xQ, HD324, PM702, Kandil, PM217, HDA337, HDH23 ve VU33 biber çeşitleri kullanılmıştır. Phytophthora capsici Leon, 'a dayanıklılıkta heterozis etkisinin araştırıldığı bu çalışmada yedi ebeveyn ve onların diallel melezleri toplam 36 bitki materyali kullanılmıştır. Phytophthora capsici Leon, 'a dayanıklılıkta kriteri olarak kesik gövde ucu yöntemi ile etmenle bulaştırılan bitki materyallerinde son nekroz uzunluğu kullanılmıştır. Ebeveynlerde son nekroz uzunluğu ortalama 102,3 mm iken, kibritler de ortalama 110,1 mm olarak belirlenmiştir. Son nekroz uzunluğunda ortalama %7,73 negatif heterozis etkisi gözlenmiştir. Phytophthora capsici Leon.'a dayanıklılıkta 23 hibritte negatif heterozis belirlenirken 5 kibritte heterozis etkisi pozitif olarak gözlemlenmiştir. Söz konusu etmene karşı dayanıklılığın ebeveynlerden kibritlere azalarak geçtiği saptanmış ve tam bir dayanıklılık elde edilememiştir. Sonuç olarak, dayanıklı çeşit oluşturmada tek bir da...
In this study the effects of irradiation doses, maternal genotypes and season on fruit set, seed ... more In this study the effects of irradiation doses, maternal genotypes and season on fruit set, seed production and parthenogenetic embryo induction after pollination by irradiated pollen in cucumber were investigated. Qamar F1, Seraset F1, Dere and Çengelköy cultivars were pollinated throughout the years with the use of pollen exposed to gamma irradiation at 300, 450 and 600 Gy. The irradiation doses did not affect fruit set or seed production. Hybrid genotypes set more fruits than open pollinated ones. Qamar F1 cultivar had the highest seed production. The highest seed production were realised in June. A 300-Gy dose led the highest haploid embryo induction for two successive years. It appeared that the higher the irradiation doses the lower the haploid induction. The hot period between April and October was found to be the best season for haploid embryo induction. The highest haploid induction, however, was obtained in June or July. Most of the embryos were found in the globular stage...
Experiments were conducted during the 1993-94 rowing season in two plastic greenhouses to study t... more Experiments were conducted during the 1993-94 rowing season in two plastic greenhouses to study the effect of different night temperatures on yield and quality of tomato plants. In one of the greenhouses temperatures were allowed to a minimum of 5 °C and in the other one to a minimum of 13 °C. None of the greenhouses were heated after March. Statistical analysis of values revealed differences in average monthly pollen vitality. However, no significant difference occurred between the heated and unheated conditions, even though 11% difference in pollen vitality between the two treatments occurred in February. A significant difference by statistical means occurred between heated and unheated greenhouses as a result of pollen germination rates, which was twice higher in 13 °C greenhouse than in 5 °C greenhouse in February. Although only few differences (8 days) occurred in the first harvesting time, a 116 % higher production was observed till the end of March in the heated greenhouse (with a production of 3,717 k m-2) than in the unheated greenhouse (1,724 kg m-2). In both greenhouses, the harvest was finished at July, 20th. Total final yields obtained from heated and non heated greenhouses were 24,038 kg m-2 and 19,047 kg m-2 respectively. Fruit sizes obtained from the two conditions showed large differences in favour of the heated greenhouse during the cold season (February and March). No significant effects of the heating on water soluble dry matter (WSDM), vitamin C content of the fruit juice, acidity, and pH values of tomato fruits were recorded
In this study, three different varieties (Paris Island, Filipus and Quintus) were grown between S... more In this study, three different varieties (Paris Island, Filipus and Quintus) were grown between September 2010 and August 2011 and recorded their bolting rates, head fresh weight, head diameter, and head length. Seeds were sown in the first day of each month starting from September 1, 2010, until August 1, 2011. The average head fresh weight, head diameter, and head length were determined as plant growth parameters. Bolting percentage was also recorded. All varieties showed early bolting in spring and summer growing period. The highest average head fresh weight was acquired from Paris Island cultivar. Paris Island was showed highest head fresh weight from October to March sowing periods, and bolting did not observe from October to February sowing periods.
In this study the obtention of in vitro haploid plants from haploid embryos induced by pollinatio... more In this study the obtention of in vitro haploid plants from haploid embryos induced by pollination with irradiated pollen was investigated. The haploid embryos of four cucumber genotypes obtained in different season of the year were cultured on the E20A medium under aseptic condition in 1992-1994. The percentage of embryos that turned into plantlets, duration needed for plant formation, and in situ plantlet development were investigated. Also, the micropropagation possibility with clonning and propagation coefficient of plantlets were determined. The ability of embryos at advanced-stages to form plants was found to be higher (60 % in the first year and 80 % in the second year) than the ones at globular stage. These embryos also produced haploid plants rapidly (in 3.5-day). A higher number of haploid plants was derived from the embryos that were cultured from May to September than from the ones that were cultured in other periods of a year. A maximum of 82 % of the embryos cultured i...
Dünyada biber yetiştiriciliğini sınırlayan en önemli hastalıklardan birisi, kök boğazı yanıklığıd... more Dünyada biber yetiştiriciliğini sınırlayan en önemli hastalıklardan birisi, kök boğazı yanıklığıdır. Bazı başka türlerde olduğu gibi Capsicum annuum L. türüne ait farklı genotiplerde de etmene karşı dayanıklılık kaynakları mevcuttur. Dayanıklılık düzeyi farklı biber genotiplerinin genetik ilişkilerinin belirlenmesi, dayanıklılık ile ilgili gen/genlerin kültür çeşitlerine aktarılması açısından önemlidir. Bu çalışmada, P. capsici'ye karşı dayanıklılık düzeyi ve orijini farklı 12 dayanıklı ve 4 duyarlı biber genotipin filogenetik ilişkileri SRAP (Sequence Related Amplified Polymorphism) ve SSR (Simple SequenceRepeat) moleküler belirteçleriyle belirlenmiştir. Bunun için, 144 SRAP primer kombinasyonu ve 27 SSR primer çifti kullanılmıştır. Otuz iki SRAP primeri herhangi bir PCR ürünü vermemiştir. Elli primer kombinasyonunda yalnızca monomorfik DNA bantı oluşurken 31 primer kombinasyonu polimorfizm sağlamış toplam 254 DNA fragmenti elde edilmiş, bunların, 99'u (%39) monomorfik, 155'i (%61) polimorfik DNAbandı olarak değerlendirilmiştir. SRAP belirteçleri ile 16 biber genotipi birbirinden genetik olarak ayrılmıştır. SSR primerinin 15'i monomorfik bant oluşturmasına karşın 12 SSR primeri ile toplam 36 DNA bandı elde edilmiş bunların 33'ü (%93) polimorfik olmuştur. SSR belirteç sistemi ile 16 biber genotipinin bazıları (
Yüzüncü Yıl Üniversitesi Tarım Bilimleri Dergisi, 2016
Bu çalışmada kök boğazı yanıklığına dayanıklı biber genotiplerinin melezlenmesi ile elde edilen y... more Bu çalışmada kök boğazı yanıklığına dayanıklı biber genotiplerinin melezlenmesi ile elde edilen yeni ıslah hatlarının anaç performansları araştırılmıştır. Perennial, PBC 178, KM2-11, Criollo de Morelos 334 ve KM12 genotiplerinin kendi aralarındaki melezlenmeleri ile oluşturulan dayanıklı hatların üzerine aşılanan Balo F 1 çeşidine ait bitkilerin fide dönemindeki bitkisel özellikleri ile arazi koşullarında verim özellikleri incelenmiştir. Ayrıca açık arazide P.capsici ile bulaştırılan bitkilerin hastalanma oranları da kaydedilmiştir. Çalışmada KM211×178-102 anacı 4658 kg da-1 ile en yüksek verim değerini göstermiş ve aşılı bitkiler % 43,60 oranında hastalanmıştır. Aşılanmamış Balo F 1 çeşidi ise 3995 kg da-1 verim değerine ulaşmış, açık alanda etmen ile bulaştırılan bitkilerin tamamı hastalanmıştır. Criollo de Morelos 334 üzerine aşılanan Balo F 1 çeşidinden 3839 kg da-1 verim elde edilmiş ve bitkiler yalnızca % 0,26 oranında hastalanmıştır. Çalışmada kalem kalınlığı-anaç kalınlığı, kök ağırlığı-meyve ağırlığı, arasında pozitif yönde ilişki saptanmıştır. Anaç kalınlığı-meyve ağırlığı, kalem kalınlığı-meyve ağırlığı, kök ağırlığı-anaç kalınlığı, kök ağırlığı-yaprak sayısı arasında ise negatif yönde ilişkiler saptanmıştır.
Fusarium wilt is one of the most destructive diseases of eggplant. It is a soil borne disease and... more Fusarium wilt is one of the most destructive diseases of eggplant. It is a soil borne disease and its control is very difficult. The best way to cope with this problem is to develop and use resistant cultivars. In this study a total of thirty-nine genotypes, twenty-one cultivated eggplants (four Turkish local cultivars, two Malaysian local genotypes, eight inbreed lines and seven F 1 hybrids) and eighteen wild relatives belonging to six different species (Solanum aethiopicum, Solanum integrifolium, Solanum sisymbrifolium, Solanum torvum, Solanum violaceum, Solanum incanum) were tested for their resistance to Fusarium oxysporum f. sp. melongenae (FOM). Resistance tests were performed with root-dip inoculation methods using a virulent Turkish isolate. Four-week-old seedlings at the second true leaf stage were used in inoculation. All the genotypes of wild Solanum species used in the trial were found resistant to FOM. Among cultivated forms only two genotypes from Malaysia (LS 1934 and LS 2436) and one cultivar from Turkey (Topan 374) were found resistant; all other cultivars, inbreed lines and F 1 hybrids were found susceptible.
Summary The responses of pepper anthers to different concentrations of silver nitrate and in depe... more Summary The responses of pepper anthers to different concentrations of silver nitrate and in dependence of the donor plant growing conditions were studied for haploid embryo production via anther culture. U-247 and U-238 genotypes, which were selected from a local paprika pepper population, were used as plant materials. Flower buds were collected from the plants grown either in greenhouse or in open field conditions. Four different silver nitrate concentrations (5, 10, 15 and 20 mg l –1 ) were tested. Haploid embryos were obtained from all the concentrations tested, but with different production rates. The highest embryo formation (45.7 embryos per 100 anthers) was obtained from the genotype U-247 in the medium containing 15 mg l –1 silver nitrate. The anthers taken from the plants grown in the greenhouse produced more embryos than that of the open field conditions. Zusammenfassung Wirkung von Silbernitrat und Spenderpflanzen-Kulturbedingungen auf die Bildung haploider Embryonen aus Paprikaantheren. In dieser Arbeit wurden die Wirkungen unterschiedlicher Silbernitrat-Konzentrationen in Kombination mit den Kulturbedingungen der Spenderpflanze auf die Bildung haploider Embryonen aus Paprikaantheren in vitro untersucht. Als Pflanzmaterial dienten ausgewahlte Genotypen (U-247 und U-238) einer lokalen Paprika-Population. Die Blutenknospen wurden sowohl von Gewachshaus- als auch von Freilandpflanzen genommen. Im Versuch wurden vier unterschiedliche Silbernitrat-Konzentrationen (5, 10, 15 und 20 mg l –1 ) getestet. Haploide Embryonen wurden bei allen getesteten Konzentrationen allerdings in unterschiedlichen Mengen produziert. Die von Gewachshauspflanzen entnommenen Antheren bildeten mehr Embryonen als die aus dem Freiland. Die hochste Embryonenproduktion (45,7 Embryonen pro 100 Antheren) wurde von Antheren des Genotyps U-247 auf einem Medium mit 15 mg l –1 Sil
In this breeding program aimed at developing resistant varieties to Fusarium wilt in Turkish melo... more In this breeding program aimed at developing resistant varieties to Fusarium wilt in Turkish melon type, molecular markers and dihaploidization techniques were used. Cum334 was used as the donor parent in a backcross program and the haploid plants were obtained via pollination with the irradiated pollen from the BC3 population to shorten the breeding period. Four different embryo rescue methods were tested to extract haploid embryos. According to the results, the best methods were found to be "inspecting seeds on the light" and "sowing seeds directly to nutrient media", and a total of the 122 haploid plants were obtained. The haploid plants were screened by molecular markers for fom1 and fom2 genes. A Simple Sequence Repeat (SSR) and a Randomly Amplified Polymorphic DNA (RAPD) marker were used for fom2 gene and a Cleaved Amplified Polymorphic Sequences (CAPS) and three Sequence Characterized Amplified Region (SCAR) markers were used for fom1 gene. While SSR (SSR180) did not segregate the haploid plants the RAPD marker (OPG17) segregated those haploid plants for fom2 gene. Haploid plants were screened for fom1 by a SCAR marker (SB17). Among the tested haploids, 41% of plants were found to be resistant and the rest of 59% were susceptible to the Fusarium wilt races 0 and 2. On the other hand while 34% of plants were resistant 66% were susceptible to the races 0 and 1 of the Fusarium oxysporum f.sp. melonis.
The island of Cyprus has an extremely rich flora in terms of genetic diversity and is localized o... more The island of Cyprus has an extremely rich flora in terms of genetic diversity and is localized on two genetic diversity centers of many cultivated plants. There are many local varieties and landraces grown for centuries in the island but they are under the risk of extinction due to the pressure of modern agriculture, especially hybrid cultivars and seedling use. A project has been carried out since 2010, with the purpose of collection and conservation of local vegetable genetic materials and to establish the core of a future gene bank for this purpose at the European University of Lefke, Faculty of Agricultural Science and Technologies. In this project, all genetic material was provided as items to a "core collection" of important major and minor local vegetables cultivars. In addition, wild relatives of many vegetables species such as wild asparagus, wild watermelon, wild chard, hostess of herbs, capers, mallow and wild mustard were also collected
The consumption of wild plants gathered from nature represents an important part of human nutriti... more The consumption of wild plants gathered from nature represents an important part of human nutrition in Cyprus. Although there have been some studies, detailed ethnobotanical works in the northern part of the island are few. The aim of this study was to collect and analyse data concerning wild edible plants used as vegetables in North Cyprus. The study was carried out during the years 2010-2011 as a part of the project on the "collection, conservation and characterisation of old vegetable varieties and their wild relatives". During these surveys information on wild edible plants was gathered. As a result of the study, a total of 27 widely used wild edible plants were recorded. The most important gathering period is winter, from December to April. Leaves (52%) and young shoots (40%) are most widely used organs. Most widely used forms are as a boiled salad (72%) or cooked in oil with or without meat (48%). Some of these plants are used only by the gatherers, whilst others are also sold in markets. Asparagus ssp. (ayrelli) and Cynara cornigera (hostes) are the two most popular and high-prized marketable wild edible plants.
In this study, salinity and drought responses of 42 melon genotypes were investigated in early pl... more In this study, salinity and drought responses of 42 melon genotypes were investigated in early plant growth stage. The plants were grown in vermiculite culture with 200 mM NaCl for salinity stress and irrigation was stopped gradually for terminal-drought stress. Control plants without stress were also grown. Young plant stage (29 day-old plant) was used for screening studies. In order to identify tolerant and susceptible melon genotypes, some physiological measurements and analysis were realized: 0-5 scale evaluation for the symptomatic appearance of the plants, shoot fresh and dry weights, root fresh and dry weights, leaf number per plant, leaf area, SPAD-Chlorophyll meter reading, membrane injury index, leaf relative water content, leaf water potential, leaf osmotic potential, stomata conductance, leaf temperature, Na, K, Ca, and Cl concentrations of the shoot and root samples. For each parameter, differences in salinity and drought stresses compared to control were calculated. At...
Efficiency of in vitro methods for chromosome duplication in haploid melon is very low. This stud... more Efficiency of in vitro methods for chromosome duplication in haploid melon is very low. This study was conducted to find a new efficient in vivo method for chromosome duplication of haploid melon (Cucumis melo) plants. Tested techniques were (i) colchicine application to lateral buds by medicine dropper, (ii) by colchicine containing agar, (iii) by colchicine imbibed cotton and (iv) shoot tip immersing in colchicine solutions. After treatments, ploidy level of plants were determined morphologically (leaf and flower dimension, presence or absence of pollen) and measured by flow cytometry. The results showed that among the tested methods, "shoot tip immersing into colchicine solutions" was the most successful technique. Dihaplodization efficiency by shoot tip immersing was about % 91.67. Duplication rate was four times more than application by cotton, ten times more than application by agar. Colchicine treatment by medicine dropper was not found as an efficient technique for chromosome duplication.
Bu çalışmada ebeveyn olarak RP40xQ, HD324, PM702, Kandil, PM217, HDA337, HDH23 ve VU33 biber çeşi... more Bu çalışmada ebeveyn olarak RP40xQ, HD324, PM702, Kandil, PM217, HDA337, HDH23 ve VU33 biber çeşitleri kullanılmıştır. Phytophthora capsici Leon, 'a dayanıklılıkta heterozis etkisinin araştırıldığı bu çalışmada yedi ebeveyn ve onların diallel melezleri toplam 36 bitki materyali kullanılmıştır. Phytophthora capsici Leon, 'a dayanıklılıkta kriteri olarak kesik gövde ucu yöntemi ile etmenle bulaştırılan bitki materyallerinde son nekroz uzunluğu kullanılmıştır. Ebeveynlerde son nekroz uzunluğu ortalama 102,3 mm iken, kibritler de ortalama 110,1 mm olarak belirlenmiştir. Son nekroz uzunluğunda ortalama %7,73 negatif heterozis etkisi gözlenmiştir. Phytophthora capsici Leon.'a dayanıklılıkta 23 hibritte negatif heterozis belirlenirken 5 kibritte heterozis etkisi pozitif olarak gözlemlenmiştir. Söz konusu etmene karşı dayanıklılığın ebeveynlerden kibritlere azalarak geçtiği saptanmış ve tam bir dayanıklılık elde edilememiştir. Sonuç olarak, dayanıklı çeşit oluşturmada tek bir da...
In this study the effects of irradiation doses, maternal genotypes and season on fruit set, seed ... more In this study the effects of irradiation doses, maternal genotypes and season on fruit set, seed production and parthenogenetic embryo induction after pollination by irradiated pollen in cucumber were investigated. Qamar F1, Seraset F1, Dere and Çengelköy cultivars were pollinated throughout the years with the use of pollen exposed to gamma irradiation at 300, 450 and 600 Gy. The irradiation doses did not affect fruit set or seed production. Hybrid genotypes set more fruits than open pollinated ones. Qamar F1 cultivar had the highest seed production. The highest seed production were realised in June. A 300-Gy dose led the highest haploid embryo induction for two successive years. It appeared that the higher the irradiation doses the lower the haploid induction. The hot period between April and October was found to be the best season for haploid embryo induction. The highest haploid induction, however, was obtained in June or July. Most of the embryos were found in the globular stage...
Experiments were conducted during the 1993-94 rowing season in two plastic greenhouses to study t... more Experiments were conducted during the 1993-94 rowing season in two plastic greenhouses to study the effect of different night temperatures on yield and quality of tomato plants. In one of the greenhouses temperatures were allowed to a minimum of 5 °C and in the other one to a minimum of 13 °C. None of the greenhouses were heated after March. Statistical analysis of values revealed differences in average monthly pollen vitality. However, no significant difference occurred between the heated and unheated conditions, even though 11% difference in pollen vitality between the two treatments occurred in February. A significant difference by statistical means occurred between heated and unheated greenhouses as a result of pollen germination rates, which was twice higher in 13 °C greenhouse than in 5 °C greenhouse in February. Although only few differences (8 days) occurred in the first harvesting time, a 116 % higher production was observed till the end of March in the heated greenhouse (with a production of 3,717 k m-2) than in the unheated greenhouse (1,724 kg m-2). In both greenhouses, the harvest was finished at July, 20th. Total final yields obtained from heated and non heated greenhouses were 24,038 kg m-2 and 19,047 kg m-2 respectively. Fruit sizes obtained from the two conditions showed large differences in favour of the heated greenhouse during the cold season (February and March). No significant effects of the heating on water soluble dry matter (WSDM), vitamin C content of the fruit juice, acidity, and pH values of tomato fruits were recorded
In this study, three different varieties (Paris Island, Filipus and Quintus) were grown between S... more In this study, three different varieties (Paris Island, Filipus and Quintus) were grown between September 2010 and August 2011 and recorded their bolting rates, head fresh weight, head diameter, and head length. Seeds were sown in the first day of each month starting from September 1, 2010, until August 1, 2011. The average head fresh weight, head diameter, and head length were determined as plant growth parameters. Bolting percentage was also recorded. All varieties showed early bolting in spring and summer growing period. The highest average head fresh weight was acquired from Paris Island cultivar. Paris Island was showed highest head fresh weight from October to March sowing periods, and bolting did not observe from October to February sowing periods.
In this study the obtention of in vitro haploid plants from haploid embryos induced by pollinatio... more In this study the obtention of in vitro haploid plants from haploid embryos induced by pollination with irradiated pollen was investigated. The haploid embryos of four cucumber genotypes obtained in different season of the year were cultured on the E20A medium under aseptic condition in 1992-1994. The percentage of embryos that turned into plantlets, duration needed for plant formation, and in situ plantlet development were investigated. Also, the micropropagation possibility with clonning and propagation coefficient of plantlets were determined. The ability of embryos at advanced-stages to form plants was found to be higher (60 % in the first year and 80 % in the second year) than the ones at globular stage. These embryos also produced haploid plants rapidly (in 3.5-day). A higher number of haploid plants was derived from the embryos that were cultured from May to September than from the ones that were cultured in other periods of a year. A maximum of 82 % of the embryos cultured i...
Dünyada biber yetiştiriciliğini sınırlayan en önemli hastalıklardan birisi, kök boğazı yanıklığıd... more Dünyada biber yetiştiriciliğini sınırlayan en önemli hastalıklardan birisi, kök boğazı yanıklığıdır. Bazı başka türlerde olduğu gibi Capsicum annuum L. türüne ait farklı genotiplerde de etmene karşı dayanıklılık kaynakları mevcuttur. Dayanıklılık düzeyi farklı biber genotiplerinin genetik ilişkilerinin belirlenmesi, dayanıklılık ile ilgili gen/genlerin kültür çeşitlerine aktarılması açısından önemlidir. Bu çalışmada, P. capsici'ye karşı dayanıklılık düzeyi ve orijini farklı 12 dayanıklı ve 4 duyarlı biber genotipin filogenetik ilişkileri SRAP (Sequence Related Amplified Polymorphism) ve SSR (Simple SequenceRepeat) moleküler belirteçleriyle belirlenmiştir. Bunun için, 144 SRAP primer kombinasyonu ve 27 SSR primer çifti kullanılmıştır. Otuz iki SRAP primeri herhangi bir PCR ürünü vermemiştir. Elli primer kombinasyonunda yalnızca monomorfik DNA bantı oluşurken 31 primer kombinasyonu polimorfizm sağlamış toplam 254 DNA fragmenti elde edilmiş, bunların, 99'u (%39) monomorfik, 155'i (%61) polimorfik DNAbandı olarak değerlendirilmiştir. SRAP belirteçleri ile 16 biber genotipi birbirinden genetik olarak ayrılmıştır. SSR primerinin 15'i monomorfik bant oluşturmasına karşın 12 SSR primeri ile toplam 36 DNA bandı elde edilmiş bunların 33'ü (%93) polimorfik olmuştur. SSR belirteç sistemi ile 16 biber genotipinin bazıları (
Yüzüncü Yıl Üniversitesi Tarım Bilimleri Dergisi, 2016
Bu çalışmada kök boğazı yanıklığına dayanıklı biber genotiplerinin melezlenmesi ile elde edilen y... more Bu çalışmada kök boğazı yanıklığına dayanıklı biber genotiplerinin melezlenmesi ile elde edilen yeni ıslah hatlarının anaç performansları araştırılmıştır. Perennial, PBC 178, KM2-11, Criollo de Morelos 334 ve KM12 genotiplerinin kendi aralarındaki melezlenmeleri ile oluşturulan dayanıklı hatların üzerine aşılanan Balo F 1 çeşidine ait bitkilerin fide dönemindeki bitkisel özellikleri ile arazi koşullarında verim özellikleri incelenmiştir. Ayrıca açık arazide P.capsici ile bulaştırılan bitkilerin hastalanma oranları da kaydedilmiştir. Çalışmada KM211×178-102 anacı 4658 kg da-1 ile en yüksek verim değerini göstermiş ve aşılı bitkiler % 43,60 oranında hastalanmıştır. Aşılanmamış Balo F 1 çeşidi ise 3995 kg da-1 verim değerine ulaşmış, açık alanda etmen ile bulaştırılan bitkilerin tamamı hastalanmıştır. Criollo de Morelos 334 üzerine aşılanan Balo F 1 çeşidinden 3839 kg da-1 verim elde edilmiş ve bitkiler yalnızca % 0,26 oranında hastalanmıştır. Çalışmada kalem kalınlığı-anaç kalınlığı, kök ağırlığı-meyve ağırlığı, arasında pozitif yönde ilişki saptanmıştır. Anaç kalınlığı-meyve ağırlığı, kalem kalınlığı-meyve ağırlığı, kök ağırlığı-anaç kalınlığı, kök ağırlığı-yaprak sayısı arasında ise negatif yönde ilişkiler saptanmıştır.
Fusarium wilt is one of the most destructive diseases of eggplant. It is a soil borne disease and... more Fusarium wilt is one of the most destructive diseases of eggplant. It is a soil borne disease and its control is very difficult. The best way to cope with this problem is to develop and use resistant cultivars. In this study a total of thirty-nine genotypes, twenty-one cultivated eggplants (four Turkish local cultivars, two Malaysian local genotypes, eight inbreed lines and seven F 1 hybrids) and eighteen wild relatives belonging to six different species (Solanum aethiopicum, Solanum integrifolium, Solanum sisymbrifolium, Solanum torvum, Solanum violaceum, Solanum incanum) were tested for their resistance to Fusarium oxysporum f. sp. melongenae (FOM). Resistance tests were performed with root-dip inoculation methods using a virulent Turkish isolate. Four-week-old seedlings at the second true leaf stage were used in inoculation. All the genotypes of wild Solanum species used in the trial were found resistant to FOM. Among cultivated forms only two genotypes from Malaysia (LS 1934 and LS 2436) and one cultivar from Turkey (Topan 374) were found resistant; all other cultivars, inbreed lines and F 1 hybrids were found susceptible.
Summary The responses of pepper anthers to different concentrations of silver nitrate and in depe... more Summary The responses of pepper anthers to different concentrations of silver nitrate and in dependence of the donor plant growing conditions were studied for haploid embryo production via anther culture. U-247 and U-238 genotypes, which were selected from a local paprika pepper population, were used as plant materials. Flower buds were collected from the plants grown either in greenhouse or in open field conditions. Four different silver nitrate concentrations (5, 10, 15 and 20 mg l –1 ) were tested. Haploid embryos were obtained from all the concentrations tested, but with different production rates. The highest embryo formation (45.7 embryos per 100 anthers) was obtained from the genotype U-247 in the medium containing 15 mg l –1 silver nitrate. The anthers taken from the plants grown in the greenhouse produced more embryos than that of the open field conditions. Zusammenfassung Wirkung von Silbernitrat und Spenderpflanzen-Kulturbedingungen auf die Bildung haploider Embryonen aus Paprikaantheren. In dieser Arbeit wurden die Wirkungen unterschiedlicher Silbernitrat-Konzentrationen in Kombination mit den Kulturbedingungen der Spenderpflanze auf die Bildung haploider Embryonen aus Paprikaantheren in vitro untersucht. Als Pflanzmaterial dienten ausgewahlte Genotypen (U-247 und U-238) einer lokalen Paprika-Population. Die Blutenknospen wurden sowohl von Gewachshaus- als auch von Freilandpflanzen genommen. Im Versuch wurden vier unterschiedliche Silbernitrat-Konzentrationen (5, 10, 15 und 20 mg l –1 ) getestet. Haploide Embryonen wurden bei allen getesteten Konzentrationen allerdings in unterschiedlichen Mengen produziert. Die von Gewachshauspflanzen entnommenen Antheren bildeten mehr Embryonen als die aus dem Freiland. Die hochste Embryonenproduktion (45,7 Embryonen pro 100 Antheren) wurde von Antheren des Genotyps U-247 auf einem Medium mit 15 mg l –1 Sil
In this breeding program aimed at developing resistant varieties to Fusarium wilt in Turkish melo... more In this breeding program aimed at developing resistant varieties to Fusarium wilt in Turkish melon type, molecular markers and dihaploidization techniques were used. Cum334 was used as the donor parent in a backcross program and the haploid plants were obtained via pollination with the irradiated pollen from the BC3 population to shorten the breeding period. Four different embryo rescue methods were tested to extract haploid embryos. According to the results, the best methods were found to be "inspecting seeds on the light" and "sowing seeds directly to nutrient media", and a total of the 122 haploid plants were obtained. The haploid plants were screened by molecular markers for fom1 and fom2 genes. A Simple Sequence Repeat (SSR) and a Randomly Amplified Polymorphic DNA (RAPD) marker were used for fom2 gene and a Cleaved Amplified Polymorphic Sequences (CAPS) and three Sequence Characterized Amplified Region (SCAR) markers were used for fom1 gene. While SSR (SSR180) did not segregate the haploid plants the RAPD marker (OPG17) segregated those haploid plants for fom2 gene. Haploid plants were screened for fom1 by a SCAR marker (SB17). Among the tested haploids, 41% of plants were found to be resistant and the rest of 59% were susceptible to the Fusarium wilt races 0 and 2. On the other hand while 34% of plants were resistant 66% were susceptible to the races 0 and 1 of the Fusarium oxysporum f.sp. melonis.
The island of Cyprus has an extremely rich flora in terms of genetic diversity and is localized o... more The island of Cyprus has an extremely rich flora in terms of genetic diversity and is localized on two genetic diversity centers of many cultivated plants. There are many local varieties and landraces grown for centuries in the island but they are under the risk of extinction due to the pressure of modern agriculture, especially hybrid cultivars and seedling use. A project has been carried out since 2010, with the purpose of collection and conservation of local vegetable genetic materials and to establish the core of a future gene bank for this purpose at the European University of Lefke, Faculty of Agricultural Science and Technologies. In this project, all genetic material was provided as items to a "core collection" of important major and minor local vegetables cultivars. In addition, wild relatives of many vegetables species such as wild asparagus, wild watermelon, wild chard, hostess of herbs, capers, mallow and wild mustard were also collected
The consumption of wild plants gathered from nature represents an important part of human nutriti... more The consumption of wild plants gathered from nature represents an important part of human nutrition in Cyprus. Although there have been some studies, detailed ethnobotanical works in the northern part of the island are few. The aim of this study was to collect and analyse data concerning wild edible plants used as vegetables in North Cyprus. The study was carried out during the years 2010-2011 as a part of the project on the "collection, conservation and characterisation of old vegetable varieties and their wild relatives". During these surveys information on wild edible plants was gathered. As a result of the study, a total of 27 widely used wild edible plants were recorded. The most important gathering period is winter, from December to April. Leaves (52%) and young shoots (40%) are most widely used organs. Most widely used forms are as a boiled salad (72%) or cooked in oil with or without meat (48%). Some of these plants are used only by the gatherers, whilst others are also sold in markets. Asparagus ssp. (ayrelli) and Cynara cornigera (hostes) are the two most popular and high-prized marketable wild edible plants.
In this study, salinity and drought responses of 42 melon genotypes were investigated in early pl... more In this study, salinity and drought responses of 42 melon genotypes were investigated in early plant growth stage. The plants were grown in vermiculite culture with 200 mM NaCl for salinity stress and irrigation was stopped gradually for terminal-drought stress. Control plants without stress were also grown. Young plant stage (29 day-old plant) was used for screening studies. In order to identify tolerant and susceptible melon genotypes, some physiological measurements and analysis were realized: 0-5 scale evaluation for the symptomatic appearance of the plants, shoot fresh and dry weights, root fresh and dry weights, leaf number per plant, leaf area, SPAD-Chlorophyll meter reading, membrane injury index, leaf relative water content, leaf water potential, leaf osmotic potential, stomata conductance, leaf temperature, Na, K, Ca, and Cl concentrations of the shoot and root samples. For each parameter, differences in salinity and drought stresses compared to control were calculated. At...
Efficiency of in vitro methods for chromosome duplication in haploid melon is very low. This stud... more Efficiency of in vitro methods for chromosome duplication in haploid melon is very low. This study was conducted to find a new efficient in vivo method for chromosome duplication of haploid melon (Cucumis melo) plants. Tested techniques were (i) colchicine application to lateral buds by medicine dropper, (ii) by colchicine containing agar, (iii) by colchicine imbibed cotton and (iv) shoot tip immersing in colchicine solutions. After treatments, ploidy level of plants were determined morphologically (leaf and flower dimension, presence or absence of pollen) and measured by flow cytometry. The results showed that among the tested methods, "shoot tip immersing into colchicine solutions" was the most successful technique. Dihaplodization efficiency by shoot tip immersing was about % 91.67. Duplication rate was four times more than application by cotton, ten times more than application by agar. Colchicine treatment by medicine dropper was not found as an efficient technique for chromosome duplication.
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