Ceska gynekologie / Ceska lekarska spolecnost J. Ev. Purkyne
An overwiev of the genetic aspects of pelvic floor defects and stress urinary incontinence in wom... more An overwiev of the genetic aspects of pelvic floor defects and stress urinary incontinence in women. A review article. Department of Gynecology and Obstetrics, Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava, Slovak Republic. An analysis of the literature using database search engines PubMed, BLAST and Ensembl in field of POP and SUI. Pelvic organ prolapse (POP) and stress urinary incontinence (SUI) affect a large number of women and often requires surgical correction. Moreover, these diseases are not only individual health but also social and economic burden on patients and society. It is possible to prevent or at least timely intervene these pathologies by screening of risk patients. The incidence of these disorders leads to find possible genetic factors. In ethiopathogenetic studies of pelvic floor defects and stress urinary incontinence it is looking for genes associated with these diseases and optimization of molecular biology methods is necessary to ...
Ceska gynekologie / Ceska lekarska spolecnost J. Ev. Purkyne
To evaluate effectiveness of electronic fetal monitoring methods during labor. A case series stud... more To evaluate effectiveness of electronic fetal monitoring methods during labor. A case series study. Department of Gynecology and Obstetrics, Jessenius Faculty of Medicine, Comenius University, Martin, Slovak Republic. We followed 112 fetuses with simultaneous continuous monitoring (cardiotocography (CTG), intrapartal fetal pulse oxymetry (IFPO), and analysis of ST segment in fetal electrocardiogram (STAN)) during labor. We determined the effective time for every diagnostic tool. Statistics: histograms, Kolmogorov-Smirnov test, Spearman's coefficient; significance (alfa): p<0.05. The effective time decreased significantly in a row: CTG > STAN > IFPO (94.8% +/- 15.1% vs. 90.4% +/- 18.3% vs. 87.4% +/- 21.2%). STAN was the most effective tool in the second stage of labor (91.3% +/- 9.4%). The most effective fetal monitoring tool is CTG. However STAN is the best diagnostic method in the second stage of labor, because of its bio-signal quality.
Ceska gynekologie / Ceska lekarska spolecnost J. Ev. Purkyne
Review of actual trends in early prediction of preeclampsia. Literature review. Department of Gyn... more Review of actual trends in early prediction of preeclampsia. Literature review. Department of Gynecology and Obstetrics, Jessenius Faculty of Medicine, Comenius University, Martin, Slovak Republic. Literature review of markers used in early prediction of preeclampsia. Review of their reliability and possible advantage of combination. Preeclampsia is a major cause of maternal and perinatal morbidity and mortality worldwide. Onset of clinic symptomes of preeclampsia can appear after 20 weeks of gestation. To find a single marker, or their combination, in early prediction of preeclampsia is one of the goals of modern perinatology. We look at some of the potential markers, which can fulfil these criteria.
Ceska gynekologie / Ceska lekarska spolecnost J. Ev. Purkyne
Objective: An overwiev of the new diagnostic method of fetal wellbeing - fetal magnetocardiograph... more Objective: An overwiev of the new diagnostic method of fetal wellbeing - fetal magnetocardiography (fMCG).Design: A review article.Setting: Department of Gynecology and Obstetrics, Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava, Slovak Republic.Methods: An analysis of the literature using database search engines PubMed, and SCOPE in field of fMCG.Results: Fetal magnetocardiography is a non-invasive technique able to monitor the spontaneous electrophy-siological activity of the fetal heart. Compared to cardiotocography and fetal electrocardiography, this is a more effective method with a higher resolution. The signal obtained from the fetal heart is sufficiently precise and the quality allows an assessment of PQRST complex alterations, and to detect fetal arrhythmia. Thanks to early diagnosis of fetal arrhythmia, there is the possibility for appropriate therapeutic intervention and the reduction of unexplained fetal death in late gestation. fMCG with high ...
Introduction: Based on many scientific research studies, there has been demonstrated a relation b... more Introduction: Based on many scientific research studies, there has been demonstrated a relation between smoking of pregnant women and its negative effects on pregnancy, intrauterine fetal growth, postnatal newborn condition, and development. The aim of our study is to highlight the adverse effects of cigarette use during pregnancy and to evaluate the effect of maternal smoking on the levels of erythropoietin (EPO) in umbilical cord plasma. Our aim was to show an effect of maternal smoking by determining carboxyhemoglobin (COHb) levels in maternal blood, too. Design: Prospective analysis. Setting: : Our study included 174 newborns. Mothers were divided into group of smokers (n = 67) and non-smokers (n = 107). This prospective study was realized from February 2012 to November 2013 at the Department of Gynecology and Obstetrics, Jessenius Faculty of Medicine in Martin, Slovak Republic. Patients signed a written informed consent and completed a questionnaire that involved medical history, and data of their smoking habits. EPO levels were measured in 174 samples of umbilical cord plasma. Infants were born spontaneously at term. Results: We observed significantly higher EPO levels in umbilical cord plasma in children born to smokers in contrast to non-smokers' (children of nonsmoking mothers: 19 ± 8 mIU/mL; children of smokers: 61 ± 33 mIU/mL, p < 0.01). The COHb levels were significantly higher in smokers (non-smokers: 0.8% ± 0.4%; smokers: 3.6% ± 1.1%, p < * Corresponding author. P. Šumichrastová et al.
Seminars in thrombosis and hemostasis, Jan 19, 2015
Introduction The aim of this study was to evaluate the genetic variability of selected single nuc... more Introduction The aim of this study was to evaluate the genetic variability of selected single nucleotide polymorphisms (SNPs) within GAS6 and PEAR1 genes and explore the association between selected SNPs and risk for fetal loss in women with sticky platelet syndrome (SPS). Materials and Methods We examined 23 female patients with SPS and history of spontaneous abortion, and 42 healthy women who served as controls. The diagnosis of SPS was established by light transmission aggregometry according to methods and criteria developed by Mammen et al. We also assessed four SNPs within the GAS6 gene (rs7400002, rs1803628, rs8191974, rs9550270) and two SNPs within PEAR1 gene (rs12041331, rs12566888). Results We identified two SNPs within PEAR1 gene (rs12041331, rs12566888) and one SNP within GAS6 gene (rs9550270) that have higher occurrence in SPS patients with history of abortion. An increased risk for abortion was observed in carriers of the rs7400002 within GAS6 gene. Conversely, we found...
Journal of obstetrics and gynaecology : the journal of the Institute of Obstetrics and Gynaecology, Jan 17, 2014
The aim of this study was to compare the activity of coagulation factor XI (FXI) between patients... more The aim of this study was to compare the activity of coagulation factor XI (FXI) between patients with spontaneous miscarriage versus control group with no history of miscarriage and thrombosis, and then we evaluated the occurrence of risk alleles in the relation to miscarriage. FXI activity was determined using a coagulometer (Sysmex, CA 1500, Japan). Single nucleotide polymorphisms (SNPs) of F11 and CYP4V2 genes were evaluated. We examined 55 patients versus 31 control subjects. We found significantly higher activity of FXI (p = 0.04) in patients versus control subjects. The occurrence of two SNPs (rs2289252 and rs2036914) of the F11 gene and SNP (rs13146272) of CYP4V2 gene was not significantly different between both groups. Increased activity of FXI may be a potential risk factor for miscarriage. High activity of FXI diagnosed in women with history of miscarriage is not probably caused by the presence of studied SNPs.
Ceská gynekologie / Ceská lékarská spolecnost J. Ev. Purkyne, 2013
To introduce QF-PCR method for detection of the most common chromosomal (trisomy 21, 18 and 13) a... more To introduce QF-PCR method for detection of the most common chromosomal (trisomy 21, 18 and 13) and gonosomal aneuploidies at our department in the second-trimester amniotic fluid. To test the hypothesis of chromosomal aneuploidies detection using STR markers of Aneufast® kit via analysing free fetal DNA (ffDNA) isolated from plasma of pregnant women with confirmed trisomy 21 in fetus. A prospective clinical study. Department of Obstetrics and Gynecology, Jessenius Faculty of Medicine and University Hospital in Martin, Slovak Republic. The samples of amniotic fluid were obtained from 67 women (twin pregnancy in 3 cases) in the 2nd trimester (15th to 22nd gestational week (g.w.)). Samples were examined using multiplex QF-PCR via Aneufast kit. In the case of positivity for trisomy 21, they were re-examined using Devyser Resolution 21 kit. All samples were parallelly evaluated by cytogenetic karyotyping. We also analyzed ffDNA from the plasma of 3 high-risk women using Aneufast kit. Th...
Ceská gynekologie / Ceská lékarská spolecnost J. Ev. Purkyne, 2011
To evaluate validity of biochemical diagnostic methods of fetal hypoxia. A case-control study. De... more To evaluate validity of biochemical diagnostic methods of fetal hypoxia. A case-control study. Department of Gynecology and Obstetrics, Jessenius Faculty of Medicine, Comenius University, Martin, Slovak Republic. We included 67 patients, and they were retrospectively divided into group of controls (n=36), and studied group (n=31) according to pH in umbilical artery (UA) <7.15. Acid-base parameters were assessed with Rapidlab 248, Bayer Healthcare LLC, East Walpole, USA. We determined criterion for metabolic acidosis (MAC) as pH UA <7.15, resp. base deficit (BD) UA >12 mmol/l. Postpartal lactate concentration in umbilical vein (UV) and UA was determined with lactatemeter Accutrend Lactate, Roche Diagnostics, Switzerland. Quantitative assessment of fetal human protein S100B was provided with ELISA (Sangtec 100 ELISA, DiaSorin Inc., Stillwater, Minnesota, USA). Fetal erythropoietin concentration in UV was examined with immunoenzymatic assessment Access EPO (Beckman Coulter, In...
Ceská gynekologie / Ceská lékarská spolecnost J. Ev. Purkyne, 2011
To evaluate validity of biophysical diagnostic methods of fetal hypoxia. A case-control study. De... more To evaluate validity of biophysical diagnostic methods of fetal hypoxia. A case-control study. Department of Gynecology and Obstetrics, Jessenius Faculty of Medicine, Comenius University, Martin, Slovak Republic. We divided the patients according to pH in umbilical artery (UA) < 7.15 into two groups: controls (n = 36), and studied (n = 31), retrospectively. We performed continuous simultaneous fetal monitoring with cardiotocography (CTG), fetal pulse oxymetry (IFPO), and ST segment analysis of fetal electrocardiogram (STAN). histograms, Kolmogorov-Smirnovov test, Mann-Whitney test, Spearman's rho; statistical significance: p < 0.05, Receiver Operating Characteristic curves, Area Under the Curve. The most frequent was intermediary CTG pattern (n = 32; 47.76%). Non-reassuring IFPO was in 22 cases (32.86%), and ST events were present in 4 cases (5.97%). CTG validity in prediction of pH in UA < 7.15: sensitivity 80.0%, specificity 50.0%, positive predictive value (PPV) 22.1...
Ceská gynekologie / Ceská lékarská spolecnost J. Ev. Purkyne, 2010
To evaluate effectiveness of hormonal treatment of hyperandrogenic syndrome (HAS). Prospective ra... more To evaluate effectiveness of hormonal treatment of hyperandrogenic syndrome (HAS). Prospective randomized study. Department of Gynecology and Obstetrics, Jessenius Faculty of Medicine, Comenius University, Martin, Slovak Republic. We assessed effect of one-year-long hormonal treatment on menstrual cycles (MC), clinical signs of HAS, and ultrasound finding (USG) in 90 patients with HAS. Patients were divided into three groups with 30 females: A--35 microg ethinylestradiol (EE) with 2.0 mg cyproterone acetate/day; B--30 microg EE with 2.0 mg dienogest/day; C--30 microg EE with 3.0 mg drospirenone/day. It was achieved a positive effect on MC regularity in every group during the first six months of treatment (p < 0.001). Signs of hirsutism, virilization, and USG finding were significantly better in group A. The most effective hormonal treatment in our study was the combination of 35 microg EE with 2.0 mg cyproterone acetate/day.
Ceská gynekologie / Ceská lékarská spolecnost J. Ev. Purkyne, 2010
Assessment of screening and prophylaxis of streptococci group B (GBS). Retrospective study. Depar... more Assessment of screening and prophylaxis of streptococci group B (GBS). Retrospective study. Department of Gynecology and Obstetrics, Jessenius Faculty of Medicine, Comenius University, Martin, Slovak Republic. Groups of patients: A-GBS negative (n=601), B-GBS positive (n=166), and C-unknown GBS status (n=238). We assessed 1005 deliveries; antenatal screening was done in 767 patients (166 GBS positive). Intrapartal antibiotic prophylaxis (IAP) was the most frequent in group B (75.3%), (A-10.0%, C-15.0%). The most common antibiotics: ampicillin, and cephalosporins of the 1st and 2nd generation. The interval from rupture of membranes (ROM) to the first IAP dose was significantly the shortest in group B. The longest interval from ROM to delivery was in group A (490 min.). This study shows the possibilities for improvement in GBS prophylaxis, in unknown GBS status, and preterm delivery, particularly.
Ceská gynekologie / Ceská lékarská spolecnost J. Ev. Purkyne, 2008
Authors present a review of epidemiology, ethiopathogenesis and current diagnostic approaches of ... more Authors present a review of epidemiology, ethiopathogenesis and current diagnostic approaches of single umbilical artery syndrome (SUA). They describe one case of SUA complicated with intrauterine growth restriction (IUGR), and possible management of risk pregnancy like that. Literature review and a case report. Department of Gynecology and Obstetrics, Jessenius Medical Faculty, Commenius University, Martin, Slovak Republic. The observation and management of one case with SUA and IUGR. SUA is the most frequent umbilical malformation. The finding of isolated SUA does not markedly increase perinatal morbidity and mortality, but its association with other pathologies leads to higher perinatal losses. An accurate management is still unclear (invasive antenatal diagnosis, especially), but SUA diagnosis should be a reason for more strict observation, timing of delivery, appropriate mode of delivery, and/or for more prompt intervention during pregnancy and labor. An early intervention and ...
Ceská gynekologie / Ceská lékarská spolecnost J. Ev. Purkyne, 2005
To determine serum levels of TNF-alpha (tumor necrosis factor alpha) as a prediction of endometri... more To determine serum levels of TNF-alpha (tumor necrosis factor alpha) as a prediction of endometriosis. Prospective clinical case control study. Department of Obstetrics and Gynaecology and Department of Pathology, Jessenius Faculty Hospital, Kollarova 2, Martin, Slovakia. The serum TNF-alpha was determined in women who underwent laparoscopy or laparotomy due to pelvic pain, infertility, dysmenorea or pelvic tumor. Endometriosis was confirmed histologically and classified by rAFS. On the basis of entering criteria 65 women were enrolled in this study. In 61 cases serum level of TNF-alpha was evaluated. The average serum level of TNF-alpha in the endometriotic group was 73.847 pg/ml (n=30) and without endometriosis was 21.089 pg/ml (n=31). We have found a significant statistical difference between the above mentioned groups in the medium levels of TNF-alpha (p<0.0001). We did not find statistical significance between TNF-alpha levels and in the group of women with endometriosis in ...
Ceská gynekologie / Ceská lékarská spolecnost J. Ev. Purkyne, 2005
The aim of this study was to analyze the incidence, causes and management in women with chronic p... more The aim of this study was to analyze the incidence, causes and management in women with chronic pelvic pain and to evaluate the role of laparoscopy. A prospective non-randomized clinical trial on 86 women with chronic pelvic pain. Clinic of Gynecology and Obstetrics, JMF CU Martin, Slovak Republic. A prospective clinical trial was performed on 86 patients with chronic pelvic pain, who have undergone laparoscopy from March 2003 to March 2004. Only patients with a pain history of at least 6 month were enrolled into this trial. Specific patient's history characteristics, laparoscopic and cytologic findings were reviewed and analyzed (pain interval, organic findings, preoperative ultrasound examination, previous surgical intervention, oral contraceptive usage, patient's medical history, menstrual cycle regularity, age, presence of dysmenorrhea). In all women, laparoscopy was performed under general anesthesia. During the study we have performed 309 diagnostic laparoscopic examin...
International Journal of Gynecology & Obstetrics, 2012
Methods: Sagittal T1-weighted images of pelvis at the bladder neck position were acquired with th... more Methods: Sagittal T1-weighted images of pelvis at the bladder neck position were acquired with the women in a sitting position by using an open-configuration magnetic resonance system, GE SIGNA SP/2. The distance from pubococcygeal line to bladder neck was measured. Mann-Whitney U test, Bonferroni correction (a = 0.05/3 = 0.016, a = 0.01/3 = 0.003), and 95% confidence intervals (CI) for the median difference using the Hodges-Lehmann estimator were used to compare groups. Results: The position of bladder neck from pubococcygeal line was 14.4 mm (median; 25th and 75th percentile, 10.6, 16.8) in nulliparous women, and 6.1 mm (−0.8, 12.0) in parous women; 8.8 mm (−2.0, 13.5) in parous women without stress urinary incontinence, and 4.8 mm (−1.3, 10.3) in parous women with stress urinary incontinence. The position of bladder neck was significantly lower in parous women than in nulliparous women (difference, 7.6 mm; 95% CI, 3.1-12.3 mm; P = 0.002). The position of bladder neck was also significantly lower in both parous women without and with stress urinary incontinence than in nulliparous women (difference, 5.9 mm; 95% CI, 1.2-12.1 mm; P = 0.014: difference, 8.8 mm; 95% CI, 4.2-15.0 mm; P = 0.002). Conclusions: The position of bladder neck was significantly lower in parous women than in nulliparous women. We demonstrated that pelvic relaxation was caused by childbirth.
A group of limb reduction defects is defined by congenital absence of either part of limbs. We re... more A group of limb reduction defects is defined by congenital absence of either part of limbs. We report an extremely rare case of symmetrical upper limb peromelia (absence of distal bones) and asymmetrical lower limb phocomelia (absence of long bones and a flipper-like appearance of feet) in a female fetus at the 20th week of pregnancy. No other malformations were identified. The prevalence of peromelia and phocomelia is extremely rare, and these malformations can be associated with some candidate genes (e.g. de novo apparently balanced reciprocal translocation 46,XX,t(2;12)(p25.1;q24.1)).
Ceska gynekologie / Ceska lekarska spolecnost J. Ev. Purkyne
An overwiev of the genetic aspects of pelvic floor defects and stress urinary incontinence in wom... more An overwiev of the genetic aspects of pelvic floor defects and stress urinary incontinence in women. A review article. Department of Gynecology and Obstetrics, Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava, Slovak Republic. An analysis of the literature using database search engines PubMed, BLAST and Ensembl in field of POP and SUI. Pelvic organ prolapse (POP) and stress urinary incontinence (SUI) affect a large number of women and often requires surgical correction. Moreover, these diseases are not only individual health but also social and economic burden on patients and society. It is possible to prevent or at least timely intervene these pathologies by screening of risk patients. The incidence of these disorders leads to find possible genetic factors. In ethiopathogenetic studies of pelvic floor defects and stress urinary incontinence it is looking for genes associated with these diseases and optimization of molecular biology methods is necessary to ...
Ceska gynekologie / Ceska lekarska spolecnost J. Ev. Purkyne
To evaluate effectiveness of electronic fetal monitoring methods during labor. A case series stud... more To evaluate effectiveness of electronic fetal monitoring methods during labor. A case series study. Department of Gynecology and Obstetrics, Jessenius Faculty of Medicine, Comenius University, Martin, Slovak Republic. We followed 112 fetuses with simultaneous continuous monitoring (cardiotocography (CTG), intrapartal fetal pulse oxymetry (IFPO), and analysis of ST segment in fetal electrocardiogram (STAN)) during labor. We determined the effective time for every diagnostic tool. Statistics: histograms, Kolmogorov-Smirnov test, Spearman's coefficient; significance (alfa): p<0.05. The effective time decreased significantly in a row: CTG > STAN > IFPO (94.8% +/- 15.1% vs. 90.4% +/- 18.3% vs. 87.4% +/- 21.2%). STAN was the most effective tool in the second stage of labor (91.3% +/- 9.4%). The most effective fetal monitoring tool is CTG. However STAN is the best diagnostic method in the second stage of labor, because of its bio-signal quality.
Ceska gynekologie / Ceska lekarska spolecnost J. Ev. Purkyne
Review of actual trends in early prediction of preeclampsia. Literature review. Department of Gyn... more Review of actual trends in early prediction of preeclampsia. Literature review. Department of Gynecology and Obstetrics, Jessenius Faculty of Medicine, Comenius University, Martin, Slovak Republic. Literature review of markers used in early prediction of preeclampsia. Review of their reliability and possible advantage of combination. Preeclampsia is a major cause of maternal and perinatal morbidity and mortality worldwide. Onset of clinic symptomes of preeclampsia can appear after 20 weeks of gestation. To find a single marker, or their combination, in early prediction of preeclampsia is one of the goals of modern perinatology. We look at some of the potential markers, which can fulfil these criteria.
Ceska gynekologie / Ceska lekarska spolecnost J. Ev. Purkyne
Objective: An overwiev of the new diagnostic method of fetal wellbeing - fetal magnetocardiograph... more Objective: An overwiev of the new diagnostic method of fetal wellbeing - fetal magnetocardiography (fMCG).Design: A review article.Setting: Department of Gynecology and Obstetrics, Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava, Slovak Republic.Methods: An analysis of the literature using database search engines PubMed, and SCOPE in field of fMCG.Results: Fetal magnetocardiography is a non-invasive technique able to monitor the spontaneous electrophy-siological activity of the fetal heart. Compared to cardiotocography and fetal electrocardiography, this is a more effective method with a higher resolution. The signal obtained from the fetal heart is sufficiently precise and the quality allows an assessment of PQRST complex alterations, and to detect fetal arrhythmia. Thanks to early diagnosis of fetal arrhythmia, there is the possibility for appropriate therapeutic intervention and the reduction of unexplained fetal death in late gestation. fMCG with high ...
Introduction: Based on many scientific research studies, there has been demonstrated a relation b... more Introduction: Based on many scientific research studies, there has been demonstrated a relation between smoking of pregnant women and its negative effects on pregnancy, intrauterine fetal growth, postnatal newborn condition, and development. The aim of our study is to highlight the adverse effects of cigarette use during pregnancy and to evaluate the effect of maternal smoking on the levels of erythropoietin (EPO) in umbilical cord plasma. Our aim was to show an effect of maternal smoking by determining carboxyhemoglobin (COHb) levels in maternal blood, too. Design: Prospective analysis. Setting: : Our study included 174 newborns. Mothers were divided into group of smokers (n = 67) and non-smokers (n = 107). This prospective study was realized from February 2012 to November 2013 at the Department of Gynecology and Obstetrics, Jessenius Faculty of Medicine in Martin, Slovak Republic. Patients signed a written informed consent and completed a questionnaire that involved medical history, and data of their smoking habits. EPO levels were measured in 174 samples of umbilical cord plasma. Infants were born spontaneously at term. Results: We observed significantly higher EPO levels in umbilical cord plasma in children born to smokers in contrast to non-smokers' (children of nonsmoking mothers: 19 ± 8 mIU/mL; children of smokers: 61 ± 33 mIU/mL, p < 0.01). The COHb levels were significantly higher in smokers (non-smokers: 0.8% ± 0.4%; smokers: 3.6% ± 1.1%, p < * Corresponding author. P. Šumichrastová et al.
Seminars in thrombosis and hemostasis, Jan 19, 2015
Introduction The aim of this study was to evaluate the genetic variability of selected single nuc... more Introduction The aim of this study was to evaluate the genetic variability of selected single nucleotide polymorphisms (SNPs) within GAS6 and PEAR1 genes and explore the association between selected SNPs and risk for fetal loss in women with sticky platelet syndrome (SPS). Materials and Methods We examined 23 female patients with SPS and history of spontaneous abortion, and 42 healthy women who served as controls. The diagnosis of SPS was established by light transmission aggregometry according to methods and criteria developed by Mammen et al. We also assessed four SNPs within the GAS6 gene (rs7400002, rs1803628, rs8191974, rs9550270) and two SNPs within PEAR1 gene (rs12041331, rs12566888). Results We identified two SNPs within PEAR1 gene (rs12041331, rs12566888) and one SNP within GAS6 gene (rs9550270) that have higher occurrence in SPS patients with history of abortion. An increased risk for abortion was observed in carriers of the rs7400002 within GAS6 gene. Conversely, we found...
Journal of obstetrics and gynaecology : the journal of the Institute of Obstetrics and Gynaecology, Jan 17, 2014
The aim of this study was to compare the activity of coagulation factor XI (FXI) between patients... more The aim of this study was to compare the activity of coagulation factor XI (FXI) between patients with spontaneous miscarriage versus control group with no history of miscarriage and thrombosis, and then we evaluated the occurrence of risk alleles in the relation to miscarriage. FXI activity was determined using a coagulometer (Sysmex, CA 1500, Japan). Single nucleotide polymorphisms (SNPs) of F11 and CYP4V2 genes were evaluated. We examined 55 patients versus 31 control subjects. We found significantly higher activity of FXI (p = 0.04) in patients versus control subjects. The occurrence of two SNPs (rs2289252 and rs2036914) of the F11 gene and SNP (rs13146272) of CYP4V2 gene was not significantly different between both groups. Increased activity of FXI may be a potential risk factor for miscarriage. High activity of FXI diagnosed in women with history of miscarriage is not probably caused by the presence of studied SNPs.
Ceská gynekologie / Ceská lékarská spolecnost J. Ev. Purkyne, 2013
To introduce QF-PCR method for detection of the most common chromosomal (trisomy 21, 18 and 13) a... more To introduce QF-PCR method for detection of the most common chromosomal (trisomy 21, 18 and 13) and gonosomal aneuploidies at our department in the second-trimester amniotic fluid. To test the hypothesis of chromosomal aneuploidies detection using STR markers of Aneufast® kit via analysing free fetal DNA (ffDNA) isolated from plasma of pregnant women with confirmed trisomy 21 in fetus. A prospective clinical study. Department of Obstetrics and Gynecology, Jessenius Faculty of Medicine and University Hospital in Martin, Slovak Republic. The samples of amniotic fluid were obtained from 67 women (twin pregnancy in 3 cases) in the 2nd trimester (15th to 22nd gestational week (g.w.)). Samples were examined using multiplex QF-PCR via Aneufast kit. In the case of positivity for trisomy 21, they were re-examined using Devyser Resolution 21 kit. All samples were parallelly evaluated by cytogenetic karyotyping. We also analyzed ffDNA from the plasma of 3 high-risk women using Aneufast kit. Th...
Ceská gynekologie / Ceská lékarská spolecnost J. Ev. Purkyne, 2011
To evaluate validity of biochemical diagnostic methods of fetal hypoxia. A case-control study. De... more To evaluate validity of biochemical diagnostic methods of fetal hypoxia. A case-control study. Department of Gynecology and Obstetrics, Jessenius Faculty of Medicine, Comenius University, Martin, Slovak Republic. We included 67 patients, and they were retrospectively divided into group of controls (n=36), and studied group (n=31) according to pH in umbilical artery (UA) <7.15. Acid-base parameters were assessed with Rapidlab 248, Bayer Healthcare LLC, East Walpole, USA. We determined criterion for metabolic acidosis (MAC) as pH UA <7.15, resp. base deficit (BD) UA >12 mmol/l. Postpartal lactate concentration in umbilical vein (UV) and UA was determined with lactatemeter Accutrend Lactate, Roche Diagnostics, Switzerland. Quantitative assessment of fetal human protein S100B was provided with ELISA (Sangtec 100 ELISA, DiaSorin Inc., Stillwater, Minnesota, USA). Fetal erythropoietin concentration in UV was examined with immunoenzymatic assessment Access EPO (Beckman Coulter, In...
Ceská gynekologie / Ceská lékarská spolecnost J. Ev. Purkyne, 2011
To evaluate validity of biophysical diagnostic methods of fetal hypoxia. A case-control study. De... more To evaluate validity of biophysical diagnostic methods of fetal hypoxia. A case-control study. Department of Gynecology and Obstetrics, Jessenius Faculty of Medicine, Comenius University, Martin, Slovak Republic. We divided the patients according to pH in umbilical artery (UA) < 7.15 into two groups: controls (n = 36), and studied (n = 31), retrospectively. We performed continuous simultaneous fetal monitoring with cardiotocography (CTG), fetal pulse oxymetry (IFPO), and ST segment analysis of fetal electrocardiogram (STAN). histograms, Kolmogorov-Smirnovov test, Mann-Whitney test, Spearman's rho; statistical significance: p < 0.05, Receiver Operating Characteristic curves, Area Under the Curve. The most frequent was intermediary CTG pattern (n = 32; 47.76%). Non-reassuring IFPO was in 22 cases (32.86%), and ST events were present in 4 cases (5.97%). CTG validity in prediction of pH in UA < 7.15: sensitivity 80.0%, specificity 50.0%, positive predictive value (PPV) 22.1...
Ceská gynekologie / Ceská lékarská spolecnost J. Ev. Purkyne, 2010
To evaluate effectiveness of hormonal treatment of hyperandrogenic syndrome (HAS). Prospective ra... more To evaluate effectiveness of hormonal treatment of hyperandrogenic syndrome (HAS). Prospective randomized study. Department of Gynecology and Obstetrics, Jessenius Faculty of Medicine, Comenius University, Martin, Slovak Republic. We assessed effect of one-year-long hormonal treatment on menstrual cycles (MC), clinical signs of HAS, and ultrasound finding (USG) in 90 patients with HAS. Patients were divided into three groups with 30 females: A--35 microg ethinylestradiol (EE) with 2.0 mg cyproterone acetate/day; B--30 microg EE with 2.0 mg dienogest/day; C--30 microg EE with 3.0 mg drospirenone/day. It was achieved a positive effect on MC regularity in every group during the first six months of treatment (p < 0.001). Signs of hirsutism, virilization, and USG finding were significantly better in group A. The most effective hormonal treatment in our study was the combination of 35 microg EE with 2.0 mg cyproterone acetate/day.
Ceská gynekologie / Ceská lékarská spolecnost J. Ev. Purkyne, 2010
Assessment of screening and prophylaxis of streptococci group B (GBS). Retrospective study. Depar... more Assessment of screening and prophylaxis of streptococci group B (GBS). Retrospective study. Department of Gynecology and Obstetrics, Jessenius Faculty of Medicine, Comenius University, Martin, Slovak Republic. Groups of patients: A-GBS negative (n=601), B-GBS positive (n=166), and C-unknown GBS status (n=238). We assessed 1005 deliveries; antenatal screening was done in 767 patients (166 GBS positive). Intrapartal antibiotic prophylaxis (IAP) was the most frequent in group B (75.3%), (A-10.0%, C-15.0%). The most common antibiotics: ampicillin, and cephalosporins of the 1st and 2nd generation. The interval from rupture of membranes (ROM) to the first IAP dose was significantly the shortest in group B. The longest interval from ROM to delivery was in group A (490 min.). This study shows the possibilities for improvement in GBS prophylaxis, in unknown GBS status, and preterm delivery, particularly.
Ceská gynekologie / Ceská lékarská spolecnost J. Ev. Purkyne, 2008
Authors present a review of epidemiology, ethiopathogenesis and current diagnostic approaches of ... more Authors present a review of epidemiology, ethiopathogenesis and current diagnostic approaches of single umbilical artery syndrome (SUA). They describe one case of SUA complicated with intrauterine growth restriction (IUGR), and possible management of risk pregnancy like that. Literature review and a case report. Department of Gynecology and Obstetrics, Jessenius Medical Faculty, Commenius University, Martin, Slovak Republic. The observation and management of one case with SUA and IUGR. SUA is the most frequent umbilical malformation. The finding of isolated SUA does not markedly increase perinatal morbidity and mortality, but its association with other pathologies leads to higher perinatal losses. An accurate management is still unclear (invasive antenatal diagnosis, especially), but SUA diagnosis should be a reason for more strict observation, timing of delivery, appropriate mode of delivery, and/or for more prompt intervention during pregnancy and labor. An early intervention and ...
Ceská gynekologie / Ceská lékarská spolecnost J. Ev. Purkyne, 2005
To determine serum levels of TNF-alpha (tumor necrosis factor alpha) as a prediction of endometri... more To determine serum levels of TNF-alpha (tumor necrosis factor alpha) as a prediction of endometriosis. Prospective clinical case control study. Department of Obstetrics and Gynaecology and Department of Pathology, Jessenius Faculty Hospital, Kollarova 2, Martin, Slovakia. The serum TNF-alpha was determined in women who underwent laparoscopy or laparotomy due to pelvic pain, infertility, dysmenorea or pelvic tumor. Endometriosis was confirmed histologically and classified by rAFS. On the basis of entering criteria 65 women were enrolled in this study. In 61 cases serum level of TNF-alpha was evaluated. The average serum level of TNF-alpha in the endometriotic group was 73.847 pg/ml (n=30) and without endometriosis was 21.089 pg/ml (n=31). We have found a significant statistical difference between the above mentioned groups in the medium levels of TNF-alpha (p<0.0001). We did not find statistical significance between TNF-alpha levels and in the group of women with endometriosis in ...
Ceská gynekologie / Ceská lékarská spolecnost J. Ev. Purkyne, 2005
The aim of this study was to analyze the incidence, causes and management in women with chronic p... more The aim of this study was to analyze the incidence, causes and management in women with chronic pelvic pain and to evaluate the role of laparoscopy. A prospective non-randomized clinical trial on 86 women with chronic pelvic pain. Clinic of Gynecology and Obstetrics, JMF CU Martin, Slovak Republic. A prospective clinical trial was performed on 86 patients with chronic pelvic pain, who have undergone laparoscopy from March 2003 to March 2004. Only patients with a pain history of at least 6 month were enrolled into this trial. Specific patient's history characteristics, laparoscopic and cytologic findings were reviewed and analyzed (pain interval, organic findings, preoperative ultrasound examination, previous surgical intervention, oral contraceptive usage, patient's medical history, menstrual cycle regularity, age, presence of dysmenorrhea). In all women, laparoscopy was performed under general anesthesia. During the study we have performed 309 diagnostic laparoscopic examin...
International Journal of Gynecology & Obstetrics, 2012
Methods: Sagittal T1-weighted images of pelvis at the bladder neck position were acquired with th... more Methods: Sagittal T1-weighted images of pelvis at the bladder neck position were acquired with the women in a sitting position by using an open-configuration magnetic resonance system, GE SIGNA SP/2. The distance from pubococcygeal line to bladder neck was measured. Mann-Whitney U test, Bonferroni correction (a = 0.05/3 = 0.016, a = 0.01/3 = 0.003), and 95% confidence intervals (CI) for the median difference using the Hodges-Lehmann estimator were used to compare groups. Results: The position of bladder neck from pubococcygeal line was 14.4 mm (median; 25th and 75th percentile, 10.6, 16.8) in nulliparous women, and 6.1 mm (−0.8, 12.0) in parous women; 8.8 mm (−2.0, 13.5) in parous women without stress urinary incontinence, and 4.8 mm (−1.3, 10.3) in parous women with stress urinary incontinence. The position of bladder neck was significantly lower in parous women than in nulliparous women (difference, 7.6 mm; 95% CI, 3.1-12.3 mm; P = 0.002). The position of bladder neck was also significantly lower in both parous women without and with stress urinary incontinence than in nulliparous women (difference, 5.9 mm; 95% CI, 1.2-12.1 mm; P = 0.014: difference, 8.8 mm; 95% CI, 4.2-15.0 mm; P = 0.002). Conclusions: The position of bladder neck was significantly lower in parous women than in nulliparous women. We demonstrated that pelvic relaxation was caused by childbirth.
A group of limb reduction defects is defined by congenital absence of either part of limbs. We re... more A group of limb reduction defects is defined by congenital absence of either part of limbs. We report an extremely rare case of symmetrical upper limb peromelia (absence of distal bones) and asymmetrical lower limb phocomelia (absence of long bones and a flipper-like appearance of feet) in a female fetus at the 20th week of pregnancy. No other malformations were identified. The prevalence of peromelia and phocomelia is extremely rare, and these malformations can be associated with some candidate genes (e.g. de novo apparently balanced reciprocal translocation 46,XX,t(2;12)(p25.1;q24.1)).
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