Papers by Kacung Hariyono,
There has been much research on high temperature stress in plants, especially in potato plants, h... more There has been much research on high temperature stress in plants, especially in potato plants, however we done this research because two potato cultivars Atlantic and Granola were most cultivated in our area of East Java Indonesia. The purpose of this reserach is to know the changes in biochemistry and heat shock protein (HSPs) due to high temperature stress in potato plants. In this study potato seedling cv. Atlantic and cv Granola were treated with 42 0 C for 24 hours in growth chamber. While control plants were grown on medium with temperature of 20 o C. Reducing sugar content on control plant relatively lower, in contrast on cv. Atlantic reducing sugar content increased higher than cv. Granola. Between two potatoes cultivars, treatment of high temperature cv. Granola affected on sucrose content almost twice compared to control plant. However there was slightly increasing of sucrose on cv. Atlantic. Endogenous proline content increases sharply in cv. Granola 1.34 μg/g sample due to stress at high temperature, while Atlantic cv slightly increased 0.7 μg/g sample. In control plants proline content were low 0.58 μg/g samples and 0.68 μg/g samples for Atlantic and Granola respectively. The western blotting analysis showed that HSP 18.5 was detected very strongly, while in Atlantic control plant was not detected, and on Granola was very weak. HSP 21 was detected very strongly in Atlantic which stressed by high temperature compared to the control plants. HSP 21 proteins detected almost 2 times in Atlantic. In contrast in Granola was detected almost similar between the controls and stressed.
Berkala Ilmiah Pertanian
Tanaman selada (Lactuca sativa L.) adalah salah satu komoditas hortikultura yang saat ini banyak ... more Tanaman selada (Lactuca sativa L.) adalah salah satu komoditas hortikultura yang saat ini banyak dikonsumsi oleh masyarakat Indonesia. Tanaman selada memiliki nilai ekonomis yang tinggi, memiliki bentuk dan warna menarik serta mengandung gizi yang cukup banyak sehingga berpotensi untuk dibubidayakan. Tanaman dengan kualitas yang tinggi dapat diperoleh dengan menggunakan bibit yang berkualitas tinggi pula / bibit unggul. Pertumbuhan bibit tanaman dapat ditingkatkan salah satunya yaitu dengan memanfaatkan paparan medan elektromagnetik extremely low frequency (ELF). Medan elekromagnetik ELF adalah gelombang elektromagnetik yang memiliki frekuensi sangat rendah yaitu 0 – 300 Hz. Medan elektromagnetik ELF termasuk dalam radiasi non ionisasi karena energi yang dihasilkan sangat sedikit bahkan tidak ada. Penggunaan paparan medan elektromagnetik terhadap biji dan benih selada diharapkan mampu meningkatkan pertumbuhan dan hasil produksi selada. Rancangan percobaan yang digunakan dalam peneli...
Berkala Ilmiah Pertanian
Kedelai merupakan komoditas tanaman pangan yang berperan penting bagi masyarakat. Permintaan kede... more Kedelai merupakan komoditas tanaman pangan yang berperan penting bagi masyarakat. Permintaan kedelai di masyarakat cukup tinggi dikarenakan kebutuhan kedelai untuk konsumsi terus meningkat seiring dengan bertambahnya jumlah penduduk. Komponen generatif pada tanaman kedelai sangat penting bagi tanaman kedelai untuk dapat mengetahui potensi hasil dari tanaman kedelai, sedangkan kandungan protein pada biji kedelai dapat berpengaruh pada mutu biji. Penelitian dilakukan di lahan percobaan Politeknik Negeri Jember pada bulan April hingga September. Penelitian ini menggunakan rancangan acak kelompok (RAK) yang terdiri dari lima varietas kedelai dengan 3 ulangan. Varietas kedelai yang digunakan dalam penelitian adalah varietas Unej 1, Unej 2, Wilis, Ringgit dan Malabar. Data hasil penelitian dianalisis menggunakan analisis ragam (Analysis of Varience) dan uji lanjutnya menggunakan uji Duncan Multiple Range Test dengan taraf kepercayaan 95%. Hasil analisis data penelitian menggunakan sidik r...
AGRIBIOS
Sektor perkebunan kelapa telah menjadi kontibutor terbesar bagi kegiatan ekonomi yang banyak dima... more Sektor perkebunan kelapa telah menjadi kontibutor terbesar bagi kegiatan ekonomi yang banyak dimanfaatkan hasilnya dalam pengembangan industri pengolahan, dimana salah satu pemanfaatan kelapa adalah pemanfaatan melalui penyadapan nira untuk dibuat gula merah kelapa. Potensi wilayah di Kabupaten Banyuwangi juga menghasilkan produk kelapa dan agroindustrinya yang tersebar di beberapa wilayah. Dari kondisi tersebut maka penelitian ini bertujuan untuk mengetahui bagaimanakah potensi wilayah dalam mendukung produksi gula merah kelapa serta strategi pengembangan agroindustri gula kelapa di Kabupaten Banyuwangi. Hasil penelitian menunjukkan bahwa Kecamatan Pesanggaran memiliki persentase terbesar dari seluruh agroindustri yang ada di Kabupaten Banyuwangi yakni sebesar 22,31 %dari total sebanyak 1067 agorindustri yang ada. Untuk rata – rata luas panen kelapa deres tertinggi berada di Kecamatan Kabat dengan rata – rata luas panen pada periode Tahun 2018 – 2019 sebesar 298 Ha. Produktifitas t...
Berkala Ilmiah Pertanian
Drought has a direct impact on the agricultural sector. Dry land is one type of marginal land, be... more Drought has a direct impact on the agricultural sector. Dry land is one type of marginal land, because drought causes various negative impacts on plants. The long drought causes the water requirement for plants to stagnate so that the plants experience stress or experience drought stress conditions. Drought stress is an environmental condition where plants do not receive sufficient water intake, so that plants cannot carry out the process of growth and development optimally and production decreases. One way that can be done on the problem of drought is to identify several plant varieties to see the response and ability of plants to survive under stress conditions. The purpose of this study was to determine the effect of drought conditions, to determine the effect of upland rice varieties and the interactions that occur between upland rice varieties and various drought stress conditions on growth and yield. The research method used was a Factorial Completely Randomized Design (CRD). ...
JURNAL BIOINDUSTRI, 2019
Tomato was classified as a climacteric fruit with a fast ripening period andthen followed by the ... more Tomato was classified as a climacteric fruit with a fast ripening period andthen followed by the increase of fruit softening. The inhibition of fruit softening was needed to maintain the quality and shelf life of the fruit. One of the way to inhibit the tomatoes softening was calcium application. However, the best concentration and timing of calcium application for tomato was still limited. The aimed of research was to determine the best combination of concentration and time of application of calcium chloride (CaCl2) to the physicochemical of tomato. Experiment was arranged in factorial completely randomized design (CRD) with two factors and three replications. First factor was concentration of CaCl2 which consisted of 5 levels (0 M; 0.3 M; 0.6 M; 0.9 M; 1.2 M), while the second factor was application time of CaCl2 (pre- and post-harvesting). The results showed that there was no interaction between concentration and application time of CaCl2 to the physicochemical of tomatoes. Treat...
Beans (Phaseolus vulgaris L.) is a type of nut plant originating from America which is widely use... more Beans (Phaseolus vulgaris L.) is a type of nut plant originating from America which is widely used as a vegetable and harvested at a younger age, which can be planted at an altitude of approximately 300 masl to above 1000 masl (Rukmana, 1994). The reduction of agricultural land in the city can be one of the problems in the cultivation of home-scale beans, where it is necessary to act on a cultivation system that can be used on a narrow land but the result of productivities are able to meet daily needs. One of them is by using a substrate hydroponic system which is still rarely used for beans since there is no knowledge of farmers about the suitable media types used in the substrate hydroponic system. Other effort that can be made to meet the needs of beans in urban areas is by using superior varieties. One type of beans that is widely cultivated by farmers is the creeping beans, where it can be harvested with a longer period. The purpose of this research is to find out varieties of ...
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy)
Tembakau (Nicotiana tabacum L.) merupakan salah satu tanaman komoditas perkebunan yang memiliki n... more Tembakau (Nicotiana tabacum L.) merupakan salah satu tanaman komoditas perkebunan yang memiliki nilai ekonomi cukup tinggi. Curah hujan tinggi dapat menyebabkan terjadinya genangan dan merupakan kendala utama dalam budidaya tanaman tembakau. Banyak studi telah menunjukkan bahwa genangan mempengaruhi respon morfologi, fisiologi dan anatomi pada tanaman. Kalsium berperan dalam membantu proses metabolisme tanaman saat mengalami cekaman abiotik. Pemberian kalsium pada tanaman dapat meningkatkan adaptasinya terhadap genangan. Penelitian ini bertujuan untuk mengetahui toleransi tanaman tembakau terhadap cekaman genangan pada berbagai taraf aplikasi kalsium berdasarkan respon fisiologi, morfologi serta anatominya. Penelitian ini dilaksanakan pada bulan November 2021 hingga Januari 2022 di Kebun percobaan Universal PT. Tempu Rejo menggunakan rancangan acak lengkap (RAL) faktorial. Faktor pertama adalah penggenangan tanaman tembakau pada usia yang berbeda (tanpa penggenangan/kontrol; penggen...
Agriprima : journal of applied agricultural sciences, Mar 31, 2022
Somatic embryogenesis is the process of forming plant embryos from somatic tissues. In general, t... more Somatic embryogenesis is the process of forming plant embryos from somatic tissues. In general, the stages of somatic embryo development consist of embryo initiation and proliferation, embryo maturation (globular, scutellar, and coleoptilar phases), embryo germination, and acclimatization. Indirect somatic embryo formation currently does not have a more in-depth study of the mechanism of the occurrence of somatic embryos, especially in porang plant, so we present knowledge related to the interaction of 2,4-D hormones and NAA on callus induction through histological analysis and scanning electron microscopy (SEM) analysis on embryogenic callus of porang plant (Amorphophallus muelleri). The purpose of this study was to determine the interaction effect of 2,4-D and NAA on the SE induction process and to study the stages of embryonic changes and development of the embryonic structure of porang plant. This research has been carried out for 6 months from May to October 2021. The research site was in the Agronomy Plant Tissue Culture Laboratory, Faculty of Agriculture, University of Jember. This study used a 2-factor Completely Randomized Design (CRD) with 3 replications. The first factor was 2,4-D concentrations of 0 ppm, 1 ppm, and 2 ppm, whereas the second factor was NAA concentrations of 0.5 ppm, 1 ppm, 1.5 ppm, 2 ppm, and 2.5 ppm. The results showed that the treatment of 1 ppm 2,4-D + 1.5 ppm NAA resulted in embryogenic callus and the results of histology and scanning electron microscopy (SEM) showed that the stages of the somatic embryo of porang included the preembryogenic mass, globular, scutellar, and coleoptile embryonic stages, then the coleoptile development to the bud primordia stage. At concentrations of 1 ppm 2,4-D + 1.5 ppm NAA, pre-embryogenic mass, and globular embryos were formed which had smooth surfaces.
UNEJ, Dec 3, 2015
This research aimed to obtain the best influential concentration of bacterial consortium (bioboos... more This research aimed to obtain the best influential concentration of bacterial consortium (bioboost) on the growth and yields of rice (Oryza sativa L.). The results of this research are expected to provide information about the effect of bacterial consortium concentration on the growth and yield of several rice varieties. The research was conducted from August, 2014 to December, 2014, in Villa Tegal Besar, Jember regency. The research used randomized complete block design with two factors and three replications. The first factor was variety: Situbagendit, mikongga, and ciherang. The second factor was concentration of bioboost: 0 ml, 20 ml, and 40 ml. The results showed that there was no interaction between the use of varieties and a concentration of bioboost on all parameters but was significantly different in response to concentrations of bioboost on parameters of number of tillers per panicle, the number of filled grain per panicle, grain yield per polybag, and the number of leaf chlorophyll. Concentration treatment showed high yields and a significant dose of 40 ml. This was shown in the parameter of the number of tillers per panicle by 50.56 tillers, number of filled grains per panicle that contain 410.89 grains, grain yields per polybag by 167.90 grams, and the amount of leaf chlorophyll by 43.22.
Jurnal Pengabdian Masyarakat IPTEKS, 2021
Kolesom Jawa merupakan tanaman herbal yang memiliki banyak kasiat bagi Kesehatan tubuh manusia se... more Kolesom Jawa merupakan tanaman herbal yang memiliki banyak kasiat bagi Kesehatan tubuh manusia sehingga memiliki harga yang tinggi ketika telah menjadi produk siap saji. Kolesom yang bernilai tinggi menjadi tidak bernilai diantara warga di desa Arjasa dan sekitarnya, sehingga hal ini perlu diidentifikasi penyebab permasalahan itu terjadi. Kolesom Jawa ini bisa menjadi peluang usaha baru bagi ibu rumah tangga dimasa pandemic untuk memperoleh tambahan penghasilan dari nilai ekonomi olahan tanaman kolesom Jawa. Identifikasi permasalahan suatu kondisi di dalam lingkungan dapat menggunakan berbagai macam metode.untuk mengetahui akar masalahnya sehingga bisa memperoleh solusi yang tepat. Pada kegiatan ini dilakukan analisa akar masalah menggunakan root couse analysis dengan menggunakan Teknik fishbone dan teknik 5 whys. Penerepan RCA pada penelitian ini menggunakan dua teknik yaitu teknik fishbone menggunakan kategori sehingga dapat digunakan sebagai arahan kemudian 5 why diagram untuk membantu mengidentifikasi faktor penyebabnya sehingga akar masalah mengerucut. Hasil dari kegiatan ini diperoleh bahwa Identifikasi akar masalah diperoleh dari kegiatan analisis deskripsi kualitatif melalui survey wawancara kemudian dianalisis menggunakan Teknik fishbone untuk memperoleh penyebab terjadinya masalah yang dihadapi. Teknik fishbone ini sebagai arahan kemudian dilanjutkan dengan teknik 5 whys karena dapat menunjukkan hubungan kausal sebab akibat, dan mampu menggabungkan cara berpikir aksi maupun kondisi. Akar masalah yang didapat adalah kurangnya sarana dan prasarana sebagai pusat informasi pengembangan warga. Solusi yang dilakukan adalah dalam bentuk penyuluhan berupa solusi dan praktek sebagai wadah untuk meningkatkan pengetahuan warga.
The research aimed to determine callus characteristics at different ages and cultivars of sugarca... more The research aimed to determine callus characteristics at different ages and cultivars of sugarcane, to determine the interaction between callus age and cultivar on regeneration ability, and to determine the long term regenerated cultivar. Experimental design used was complete randomized design (CRD) with 2 factors. The first factor was sugarcane cultivars (v) that comprised of 4 levels, namely: LK 95-127, LK 92-17, K 93-219, and K 93-347. The second factor was callus ages (u) which consisted of 3 levels, namely 2 months, 3 months, and 4 months. All treatments replicated ten times. Response variables observed were percentage of embryogenic callus, colour, structure and diameter of callus, percentage of shoot regeneration, the lenght of shoot and number of shoot. Most of data except colour and structure of callus were tested using analysis of variance. The significant differences between treatments was analyzed by Duncan's Multiple Range Test at 5 % significant level. Callus indu...
There has been much research on high temperature stress in plants, especially in potato plants, h... more There has been much research on high temperature stress in plants, especially in potato plants, however we done this research because two potato cultivars Atlantic and Granola were most cultivated in our area of East Java Indonesia. The purpose of this reserach is to know the changes in biochemistry and heat shock protein (HSPs) due to high temperature stress in potato plants. In this study potato seedling cv. Atlantic and cv Granola were treated with 420C for 24 hours in growth chamber. While control plants were grown on medium with temperature of 20oC. Reducing sugar content on control plant relatively lower, in contrast on cv. Atlantic reducing sugar content increased higher than cv. Granola. Between two potatoes cultivars, treatment of high temperature cv. Granola affected on sucrose content almost twice compared to control plant. However there was slightly increasing of sucrose on cv. Atlantic. Endogenous proline content increases sharply in cv. Granola 1.34 μg/g sample due to ...
Tebu merupakan akumulator silika yang berarti memerlukan banyak asupan silika. Pupuk silika (Si) ... more Tebu merupakan akumulator silika yang berarti memerlukan banyak asupan silika. Pupuk silika (Si) dapat berasal dari berbagai sumber dan terobosan teknologi saat ini tersedianya Si dalam bentuk nano. Beberapa varietas tebu unggul baru belum diuji pada lahan marginal. Tujuan penelitian ini untuk mengetahui pengaruh pemberian nano silika terhadap pertumbuhan fase bibit beberapa varietas unggul baru pada tanah entisol. Rancangan penelitian ini menggunakan Rancangan Acak Kelompok Faktorial dengan 2 faktor. Faktor pertama aplikasi nano Si (S) terdiri atas aplikasi nano Si 0% (S1) dan aplikasi nano Si 30% (S2). Faktor kedua varietas unggul baru terdiri atas Bululawang (V1), NX-01 (V2), NX-02 (V3), dan VMC 86-550 (V4). Hasil penelitian menunjukkan pemberian nano Si tidak berpengaruh nyata pada pertumbuhan tebu fase pembibitan, sedangkan varietas dan interaksi nano Si x V berpengaruh nyata terhadap pertumbuhan tebu. Varietas VMC 86-550 menunjukkan rata-rata pertumbuhan yang lebih baik pada s...
Bioscience Research 201815(3):2019-2028 (Journal by Innovative Scientific Information & Services ... more Bioscience Research 201815(3):2019-2028 (Journal by Innovative Scientific Information & Services Network)
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