Papers by Jupalle Nagaiah Naidu
Journal of Molecular and Genetic Medicine, 2014
Polycystic ovary syndrome is the most common endocrinal disorder and is associated with infertili... more Polycystic ovary syndrome is the most common endocrinal disorder and is associated with infertility. Insulin resistance is a key component in the pathogenesis of PCOS and the predisposition to Type 2 diabetes mellitus. The existing literature supports a strong basis of PCOS clusters in families. However, due to the genetic and phenotypic heterogeneity of PCOS and the lack of large cohort studies and identification of specific contributing genes to date have yielded only few conclusive results. Although several loci have been proposed as PCOS genes including CYP11A, the insulin gene, and a region near the insulin receptor, the strongest case can be made for the region near the insulin receptor gene. Insulin receptor substrates proteins (IRS) are critical for insulin mediated signal transduction in insulin target tissues. Several studies have shown that IRS-1 Gly972Arg polymorphism might be the factor causing susceptibility to Type 2 diabetes mellitus (T2DM) and are associated with phenotypic features of PCOS. In this article we reviewed the current status of the genetic analysis of insulin (INS), insulin receptor (INSR), insulin receptor substrate (IRS) in relation to polycystic ovary syndrome, an infertility disorder in females.
Relationship between estrogen and lipid profile status in postmenopausal women
International Journal of Applied Biology and P, Jul 1, 2012
ABSTRACT: Menopause is the permanent cessation of mensturation due to loss of ovarian follicular ... more ABSTRACT: Menopause is the permanent cessation of mensturation due to loss of ovarian follicular function, which results in decreased production of estradiol and other hormones. Decreased levels of estrogen leads to variations in lipid profile leading to atherosclerosis thus by increasing risk of coronary artery disease in post menopausal women. The present study was conducted to evaluate the relationship between serum estradiol and lipid profile status in postmenopausal women to assess risk for coronary artery disease. Study includes 70 subjects, 30 healthy premenopausal women (25-40yrs) and the healthy postmenopausal women in the age group of (55 – 65yrs).Serum estradiol and lipid profile status were estimated in both cases and controls. Reduced estradiol (P0.0001) in post menopausal women and p value is highly significant for total cholesterol, triglycerides, low density lipoproteins. Conclusion: Thus the study outlines that the low levels of estrogen exerts a significant effect on the plasma lipids and lipoprotein metabolism. The risk of coronary artery disease increases in women after menopause. Key words: TC-total cholesterol, TG-Triglycerides, HDL-High density lipoproteins, LDL-Low density lipoproteins, CHD-coronary heart disease.
Journal of Biosciences, 1982
Blood collected on filter paper by finger-prick gave results comparable to intravenous serum samp... more Blood collected on filter paper by finger-prick gave results comparable to intravenous serum samples when analysed by enzyme-linked immunosorbent assay (ELISA). All the 100 microfilaraemia, 5 out of 100 endemic normals and none of the 10 nonendemic normal filter paper blood samples showed the presence of filarial antibody when tested by this method,using culture antigen and anti-immunoglubulins, class G, Μ and Apenicillinase conjugate. When the same samples were screened for the presence of IgM antibody, 91 out of 100 microfilaraemia, 13 out of 100 endemic normal and none of the 10 nonendemic normal samples showed a positive reaction. Enzyme linked immunosorbent assay, using culture antigen and filter paper blood samples, appears to work in large field studies for detection of filarial infection.
Introduction: Polycystic ovary syndrome (PCOS) is the most common endocrinological disorder in wo... more Introduction: Polycystic ovary syndrome (PCOS) is the most common endocrinological disorder in women of reproductive age group. It is the leading cause of infertility in females presenting a wide range of clinical manifestations. Aim: To investigate the glycaemic status, calcium, phosphorus, insulin and 25-OH vitamin D levels in women with polycystic ovary syndrome and to compare these parameters with age and BMI matched healthy control women. To find out the correlation between insulin resistance and vitamin D among PCOS women. Materials and Methods: The study comprised of 80 newly diagnosed polycystic ovary syndrome women in the age range of 23 to 33 years. The biochemical parameters measured in the study includes fasting blood sugar, fasting insulin, calcium, phosphorus and vitamin D. The values obtained were compared with equal number of age and body mass indexed (BMI) matched healthy controls. Results and Discussion: In the present study the levels of fasting glucose, fasting i...
International Journal of Fertility and Sterility, Apr 5, 2016
Polycystic ovary syndrome (PCOS) is one of the most common endocrinological disorders among women... more Polycystic ovary syndrome (PCOS) is one of the most common endocrinological disorders among women of reproductive age and the leading cause of female infertility. This study intends to evaluate the lipid profile, hormonal levels [free T3 (fT3), free T4 (fT4), thyroid stimulating hormone (TSH), insulin, luteinizing hormone (LH), follicle stimulating hormone (FSH), and prolactin] in PCOS women from Nellore and its surrounding districts of Andhra Pradesh, India. This cross-sectional study included 80 newly diagnosed PCOS women and an equal number of age and body mass index (BMI) matched healthy controls. We used the photometry methods to determine serum glucose levels and the lipid profile. An immunoturbidometry method was employed to measure high sensitive C-reactive protein (hsCRP). All hormonal parameters were measured using chemiluminescence immunoassays. Insulin resistance was evaluated using the homeostatic model assessment-insulin resistance (HOMA-IR) method. Statistical analysi...
Association of Dyslipidemia and Inflammatory Markers in Type 2 Diabetes Mellitus
ABSTRACT: Background: The interplay between inflammation and lipids has recently been the focus o... more ABSTRACT: Background: The interplay between inflammation and lipids has recently been the focus of research aimed at understanding the development of Type 2 diabetes mellitus and the process of atherogenesis. Inflammatory markers particularly Tumor necrosis factor-α and high sensitive C-reactive protein interfere with lipid homeostasis and activates proinflammatory mechanisms in type-2 Diabetes mellitus. Our aim of the study was to analyze and correlate the inflammatory markers with lipids in Type2 Diabetes mellitus. 50 diabetic subjects and 50 normal healthy controls were recruited from the outpatient department of Narayana Medical college and hospital, Nellore, A.P). Serum TNF-alpha concentration was measured using Sandwich ELISA kit method. commercial enzymatic methods were used in the determination of serum total.cholesterol, HDL cholesterol and Triacylglycerides. LDL cholesterol was estimated using friedewald equation. Turbidimetric immunoassay (QUANTIA CRP- US) is used for ult...
Archives Medical Review Journal Review of Concepts and Controversies of Uric Acid as Antioxidant and Pro-Oxidant
Uric acid, the end product of purine catabolism in humans and is known for its crystal deposition... more Uric acid, the end product of purine catabolism in humans and is known for its crystal deposition at higher concentrations (>7 mg/dl) in gout. Less is known about its antioxidant property and the beneficial effects in various diseases. It is thought that high concentration of uric acid in humans is an evolutionary advantage and it is also hypothesized that high concentration of uric acid is to compensate the antioxidant capacity of ascorbic acid which is lost in humans during the course of evolution. In the extracelluar environment, uric acid can scavenge free radicals like hydroxyl radical, singlet oxygen and peroxynitrite radical therefore, it is considered as a powerful antioxidant. On the other hand uric acid depending upon the chemical milieu, changing its property and at times it acts as pro oxidant and is associated with the pathobiochemistry in developing various diseases like hypertension, cardio vascular diseases, ischemia reperfusion injury, diabetes mellitus, non alco...
Study of Biochemical Bone Turnover Markers in Postmenopausal Women Leading to Osteoporosis
ABSTRACT: Osteoporosis is the term used for diseases that cause a reduction in the mass of bone p... more ABSTRACT: Osteoporosis is the term used for diseases that cause a reduction in the mass of bone perunit volume and is one of the dreaded afflictions of ageing. Osteoporosis increases bone fragility and susceptibility to fractures. This silently progressing metabolic bone disease is widely prevealent in India. This study was conducted to evaluate the role of biochemical bone turnover markers in postmenopausal women to assess the risk of osteoporosis. Study includes 70 women (40 postmenopausal, 30 premenopausal women) serum estradiol, serum alkaline phosphatase, urinary hydroxyproline were estimated in both cases and controls. There is decrease in serum estrodiaol levels in postmenopausal women when compared to premenopausal women estrogen deficiency induces bone loss similarly ALP is highly significant P<0.001 in prostmenopausal women suggesting that there is high alkaline phosphate activity in postmenopausal women as a result of the inhibitory effects of estrogen on bone turnover...
Relationship Between Estrogen and Lipid Profile Status in Postmenopausal Women
ABSTRACT: Menopause is the permanent cessation of mensturation due to loss of ovarian follicular ... more ABSTRACT: Menopause is the permanent cessation of mensturation due to loss of ovarian follicular function, which results in decreased production of estradiol and other hormones. Decreased levels of estrogen leads to variations in lipid profile leading to atherosclerosis thus by increasing risk of coronary artery disease in post menopausal women. The present study was conducted to evaluate the relationship between serum estradiol and lipid profile status in postmenopausal women to assess risk for coronary artery disease. Study includes 70 subjects, 30 healthy premenopausal women (25-40yrs) and the healthy postmenopausal women in the age group of (55 – 65yrs).Serum estradiol and lipid profile status were estimated in both cases and controls. Reduced estradiol (P0.0001) in post menopausal women and p value is highly significant for total cholesterol, triglycerides, low density lipoproteins. Conclusion: Thus the study outlines that the low levels of estrogen exerts a significant effect ...
International journal of pharma and bio sciences, 2015
The Incidence of Prostate Cancer is increasing day by day all over the world. At present the Inci... more The Incidence of Prostate Cancer is increasing day by day all over the world. At present the Incidence is about 5 per 1, 00,000 in India. There is strong evidence that genetic and environmental factors play in important role in pathophyology, of prostatic cancer. In this study HOMA-IR, serum Calcium, Phosphate, Lipid profile, PSA and antioxidant vitamins were estimated. Age matched 50 Prostate cancer cases and 50 controls included in the study. The insulin resistance in all cases and control groups evaluated by estimating fasting blood glucose and insulin levels, by using HOMA IR Formula. Antioxidant vitamins estimated by HPLC, Serum Calcium, Phosphate, lipid profile and Prostate specific antigen by enzymatic kits using Autoanlyzers. In the present study, the values of HOMA-IR, serum Calcium and PSA are (p<0.05) significantly increased than in controls. The Serum alfa tocopherol and ascorbic acid values in cases significantly reduced (p<0.05) than in controls. The growing of p...
Research article ALTERATIONS OF SERUM URIC ACID CONCETRATIONS IN PREECLAMPSIA
Hypertensive disorders during pregnancy increase maternal and fetal risk. Preeclampsia is charact... more Hypertensive disorders during pregnancy increase maternal and fetal risk. Preeclampsia is characterised by hypertension and proteinuria. Increased uric acid is a key clinical feature of preeclampsia; higher levels correlate with significant maternal and fetal morbidity and mortality. The present study was undertaken to estimate serum uric acid , the end product of purine catabolism, in clinically diagnosed preeclamptic women (n=30) and the values were compared with that of normotensive pregnant women (n=30) ,aged 18-30yrs. All of them were in their third trimester and primigravida. Serum uric acid levels were measured by spectrophotometry. We observed that serum uric acid levels were increased significantly when compared with that of normotensive pregnant women (p value < 0.000).
Review of concepts and controversies of uric acid as antioxidant and pro- oxidant – an uncertainty
Archives Medical Review Journal, 2015
Uric acid, the end product of purine catabolism in humans and is known for its crystal deposition... more Uric acid, the end product of purine catabolism in humans and is known for its crystal deposition at higher concentrations (>7 mg/dl) in gout. Less is known about its antioxidant property and the beneficial effects in various diseases. It is thought that high concentration of uric acid in humans is an evolutionary advantage and it is also hypothesized that high concentration of uric acid is to compensate the antioxidant capacity of ascorbic acid which is lost in humans during the course of evolution. In the extracelluar environment, uric acid can scavenge free radicals like hydroxyl radical, singlet oxygen and peroxynitrite radical therefore, it is considered as a powerful antioxidant. On the other hand uric acid depending upon the chemical milieu, changing its property and at times it acts as pro oxidant and is associated with the pathobiochemistry in developing various diseases like hypertension, cardio vascular diseases, ischemia reperfusion injury, diabetes mellitus, non alco...
International Journal of Clinical Biochemistry and Research, 2021
Essential hypertensionis a typical example of complex, multifactorial trait and a well studied ri... more Essential hypertensionis a typical example of complex, multifactorial trait and a well studied risk factor for cardiovascular disease. hs-CRP is well known marker of inflammation resulting in atherosclerosis and cardiovascular disease. Recent studies showed that 25-OH-vitamin-D has ananti-inflammatory role and decreased levels may increase the cardiovascular risk in subjects with essential hypertension. The study included 30 adults both male and female with essential hypertension (Systolic Blood Pressure ? 140 mm Hg and Diastolic Blood Pressure ? 90 mm Hg) as cases. 30 age and sex matched healthy individuals both male & female between the age group of 24-60 years attending General OPD, General Medicine Department, NMCH, were taken as controls. The inverse relationship of Vitamin D with hs-CRP levels leads to cardiovascular risk in subjects with essential hypertension. Keywords: Essential hypertension, hs-CRP, Vitamin D and Inflammation
TNF-α can directly alter lipid metabolism through inhibition of FFA uptake and lipogenesis and stimulate free fatty release via lipolysis and therefore contributes to the development of dyslipidemia and resultant metabolic complications
Background: The interplay between inflammation and lipids has recently been the focus of research... more Background: The interplay between inflammation and lipids has recently been the focus of research aimed at understanding the development of Type 2 diabetes mellitus and the process of atherogenesis. Inflammatory markers particularly Tumor necrosis factor-α and high sensitive C-reactive protein interfere with lipid homeostasis and activates proinflammatory mechanisms in type-2 Diabetes mellitus. Our aim of the study was to analyze and correlate the inflammatory markers with lipids in Type2 Diabetes mellitus. 50 diabetic subjects and 50 normal healthy controls were recruited from the outpatient department of Narayana Medical college and hospital, Nellore, A.P). Serum TNF-alpha concentration was measured using Sandwich ELISA kit method. commercial enzymatic methods were used in the determination of serum total.cholesterol, HDL cholesterol and Triacylglycerides. LDL cholesterol was estimated using friedewald equation. Turbidimetric immunoassay (QUANTIA CRPUS) is used for ultrasensitive ...
Content-Based Image Retrieval (CBIR) uses the visual contents of an image such as color, shape, t... more Content-Based Image Retrieval (CBIR) uses the visual contents of an image such as color, shape, texture and special layout to represent and index the image. The feature vectors of images in the database form a feature database. To retrieve images, users provide the retrieval system with example images or sketched figures. The system then changed these examples into its internal representation of feature vectors. The similarity between the characteristics vectors of the query example or sketch and those of the images in the database are then calculated and retrieval is performed with the assist of an indexing scheme. The indexing proposal provides an efficient way to search for the image database. Recent retrieval systems have included users Relevance Feedback (RF) to modify the retrieval process in order to generate perceptually and semantically more meaningful retrieval results.
STUDY OF BIOCHEMICAL BONE TURNOVER MARKERS IN POSTMENOPAUSAL WOMEN LEADING TO OSTEOPOROSIS Sk.Deepthi, G.Amar
Osteoporosis is the term used for diseases that cause a reduction in the mass of bone perunit vol... more Osteoporosis is the term used for diseases that cause a reduction in the mass of bone perunit volume and is one of the dreaded afflictions of ageing. Osteoporosis increases bone fragility and susceptibility to fractures. This silently progressing metabolic bone disease is widely prevealent in India. This study was conducted to evaluate the role of biochemical bone turnover markers in postmenopausal women to assess the risk of osteoporosis. Study includes 70 women (40 postmenopausal, 30 premenopausal women) serum estradiol, serum alkaline phosphatase, urinary hydroxyproline were estimated in both cases and controls. There is decrease in serum estrodiaol levels in postmenopausal women when compared to premenopausal women estrogen deficiency induces bone loss similarly ALP is highly significant P<0.001 in prostmenopausal women suggesting that there is high alkaline phosphate activity in postmenopausal women as a result of the inhibitory effects of estrogen on bone turnover rate whic...
Tumor Necrosis Factor-Alpha in the Development of Insulin Resistance in Type 2 Diabetes Mellitus
International Journal of Applied Biology and P, 2011
Obesity and Insulin resistance are very frequent disorders and are described as the dominant risk... more Obesity and Insulin resistance are very frequent disorders and are described as the dominant risk factors for cardiovascular diseases. Recent research suggests that TNF-alpha, an adipocytokine plays a key role in the development of insulin resistance. Aim of the study was to estimate TNF-alpha levels and to investigate the association of TNF-alpha with the various factors associated with insulin resistance like Body mass index, Waist Hip Ratio, fasting insulin etc. A total of 100 subjects attending out patient department of General Medicine , Narayana Medical College , Nellore were selected for the study.50 patients were diagnosed as type-2 diabetics and 50 subjects were taken as normal healthy controls who attended for a general check-up. Anthropometric and biochemical measurements were analyzed. Serum Insulin and TNF-α were measured using chemiluminescence method and ELISA method respectively. Homeostasis model assessment score was used to gauge the level of insulin resistance. Ou...
Research Article Study of insulin resistance and antioxidant vitamin status in prostate cancer patients
The incidence of prostate cancer is 5 per 100000 in southern and eastern Asia. Both genetic and e... more The incidence of prostate cancer is 5 per 100000 in southern and eastern Asia. Both genetic and environmental factors have been implicated in its etiology. The mitogenic and growth stimulatory effects of Insulin growth factor may be involved in prostate carcinogenesis. To evaluate serum insulin and insulin resistance was passed by HOMA- IR. Prostatic specific antigen passed by immune-enzymatic assay. Vitamins were estimated by high performance liquid chromatography. In our study 30 prostate cancer patients aged 60-80years were taken as cases. 30 normal age matched disease free person were taken as controls in both groups, Insulin resistance and antioxidant vitamin status was studied. In the present study, the value of HOMA-IR was (P <0.05) is significantly higher compare to controls. Serum vitamin E and vitamin C values for cases was reduced (P <0.05) significantly lower than controls. The development of prostate cancer is a multistep process. Hyperinsulinemia associated with ...
Detection of filarial antigen in urine by sandwich ELISA & its use in diagnosis
The Indian journal of medical research, 1985
hsCRP AND FIBRINOGEN IN THE EARLY DIAGNOSIS OF ATHEROGENESIS IN MALE SMOKERS
Cigarette smoking is one of the major health hazards of the world population as it is contributin... more Cigarette smoking is one of the major health hazards of the world population as it is contributing to the cardiovascular diseases and to the increasing proportion of the sudden deaths. hsCRP and Fibrinogen are independent & signifigant markers of cardiovascular mortality. The aim of the study is to identify the earlier markers of atherogenesis in healthy adult male smokers for better risk prediction and screening purpose. 60 adult healthy men of age group 19-49 years were enrolled. 30 smokers & 30 nonsmoker. Smokers were compared with the non-smokers by doing the blood tests which included blood glucose, lipidprofile, hsCRP & Fibrinogen. Smokers had significantly higher levels of total serum cholesterol [245.03±72.82 vs160.07±21.39 p<0.000], LDL levels[149.17±48.87vs.98.67±14.93p<0.000] and triglyceride levels [152.43± 52.40vs. 94.83±17.75p<0.000]. HDL levels were low in smokers. hsCRP & Fibrinogen levels were higher in smokers [6.8±3.04vs1.38±0.67p<0.00&644.20±231.38vs3...
Uploads
Papers by Jupalle Nagaiah Naidu