Species distribution models (SDMs) are widely used in ecology and conservation. Presence-only SDM... more Species distribution models (SDMs) are widely used in ecology and conservation. Presence-only SDMs such as MaxEnt frequently use natural history collections (NHCs) as occurrence data, given their huge numbers and accessibility. NHCs are often spatially biased which may generate inaccuracies in SDMs. Here, we test how the distribution of NHCs and MaxEnt predictions relates to a spatial abundance model, based on a large plot dataset for Amazonian tree species, using inverse distance weighting (IDW). We also propose a new pipeline to deal with inconsistencies in NHCs and to limit the area of occupancy of the species. We found a significant but weak positive relationship between the distribution of NHCs and IDW for 66% of the species. The relationship between SDMs and IDW was also significant but weakly positive for 95% of the species, and sensitivity for both analyses was high. Furthermore, the pipeline removed half of the NHCs records. Presence-only SDM applications should consider th...
The heterogeneous mixture of proteins present in expressed prostatic fluid from forty-nine men ov... more The heterogeneous mixture of proteins present in expressed prostatic fluid from forty-nine men over 25 years old was analyzed on two-dimensional electrophoretic gels stained with silver. Samples were collected from men diagnosed with chronic prostatitis, urinary tract infection, benign prostatic hypertrophy (BPH), and prostatic carcinoma. Reference patterns were developed for all proteins observed in electrophoretograms. Over 1000 distinct proteins are contained in the comprehensive proteins pattern of prostatic fluid; however, only 18 proteins were common to all samples. No proteins unique to fluids from men with BPH or prostatic cancer were observed. Some proteins in the common proteins reference pattern of BPH patients were not seen in samples from men with prostatic cancer. Our preliminary results indicate that a series of proteins tentatively identified as variants of prostatic acid phosphatase appeared elevated in all BPH fluids but was absent or below limits of detection in samples from men with prostatic carcinoma. Our studies indicate that proteins secreted in prostatic fluid reflect physiological changes in the prostate, and certain changes may be useful indicators of malignant transformation.
Actas Del Congreso Iberoamericano De Las Lenguas En La Educacion Las Lenguas En La Educacion Cine Literatura Redes Y Nuevas Tecnologias 2013 Isbn 978 84 369 5528 6 Pags 241 249, 2013
Page 1. Stimulation and Promotion of Germination in Opuntia ficus-indica Seeds ♦ Mariela Altare1,... more Page 1. Stimulation and Promotion of Germination in Opuntia ficus-indica Seeds ♦ Mariela Altare1, Sinibaldo Trione1, Juan C. Guevara1, and Mariano Cony1 1Instituto Argentino de Investigaciones de las Zonas Áridas (IADIZA-CONICET) CC. 507 (5500) Mendoza, Argentina ...
In this report, we describe methods used to analyze the protein composition of sectioned frozen p... more In this report, we describe methods used to analyze the protein composition of sectioned frozen prostatic tissues by two-dimensional gel electrophoresis. Our results show a high degree of homology in twodimensional electrophoretograms of proteins extracted from frozen sec tions of malignant prostate glands. Such homology was not apparent in protein patterns of benign hypertrophie prostatic tissue sections. Typi cally, 600 discrete proteins were resolved on two-dimensional electro phoretograms and 9 proteins were present in all patterns of prostate adenocarcinomatous tissues. These nine proteins were not observed in any of the protein electrophoretograms developed from nonmalignant prostate tissue. Three proteins were found common to nonmalignant prostate glands but were not present in prostatic adenocarcinoma.
The heterogeneous mixture of proteins present in expressed prostatic fluid from forty-nine men ov... more The heterogeneous mixture of proteins present in expressed prostatic fluid from forty-nine men over 25 years old was ana lyzed on two-dimensional electrophoretic gels stained with silver. Samples were collected from men diagnosed with chronic prostatitis, urinary tract infection, benign prostatic hypertrophy (BPH), and prostatic carcinoma. Reference patterns were developed for all proteins observed in electrophoretograms. Over 1000 distinct proteins are contained in the comprehensive proteins pattern of prostatic fluid; however, only 18 proteins were common to all samples. No proteins unique to fluids from men with BPH or prostatic cancer were observed. Some proteins in the common proteins reference pattern of BPH patients were not seen in samples from men with prostatic cancer. Our preliminary results indicate that a series of proteins tentatively identified as variants of prostatic acid phosphatase appeared elevated in all BPH fluids but was absent or below limits of detection in samples from men with prostatic carcinoma. Our studies indicate that proteins secreted in prostatic fluid reflect physiological changes in the prostate, and certain changes may be useful indicators of malig nant transformation.
En este artículo se describe un sistema multiagente capaz de detectar operaciones de consulta, en... more En este artículo se describe un sistema multiagente capaz de detectar operaciones de consulta, envío y borrado de correos en un sistema de correo electrónico. El trabajo implicó el montaje y configuración del sistema de correo electrónico Exchange, la creación de un sistema multiagente -siguiendo la metodología AOPOA con agentes en los clientes y el servidor, de tal forma que permitieran detectar las operaciones de consulta-, envío y borrado de correos no autorizados, la definición de un protocolo de prueba del sistema y el análisis de los resultados, obtenidos de la aplicación del sistema multiagente. Adicionalmente, se creó un firewall que permitiera registrar las mismas operaciones de detección y comprobar el funcionamiento del sistema multiagente.
We report here new evidence of tridactyl dinosaur footprints, comprising nine isolated footprints... more We report here new evidence of tridactyl dinosaur footprints, comprising nine isolated footprints from a new locality in the Atacama Region, northern Chile. The track-bearing levels are located mainly at La Descubridora ravine and one isolated track near the intersection between La Descubridora and Carrizalillo ravines, approximately 11 km southeast from Tierra Amarilla town; the studied levels are assigned to the Pabellon Formation (Lower Cretaceous). The icnites correspond at least to two dinosaur forms, mainly ornithopods (with affinities to Hadrosauriformes dinosaurs) and theropod footprints. These are the first well-documented dinosaur icnites and the first record of ornithopod footprints from the Atacama Region. This record expands the knowledge of ornithopods in Chile, which previously were only known from the Chacarilla Formation, Tarapacá Region, the Baños del Flaco Formation in O'Higgins Region and the Dorotea Formation in Magallanes Region.
Departamento de Ingenieria Quimica, Facultad de Ciencias Fisicas y Matemiticas, Universidad de Ch... more Departamento de Ingenieria Quimica, Facultad de Ciencias Fisicas y Matemiticas, Universidad de Chile, Casilla 2777 y Centro para la Investigacih Interdisciplinaria Avanzada en Ciencias de 10s Materiales (CIMAT). Santiago, Chile. ... Departamento de Quimica Orginica y ...
Two-dimensional gel electrophoresis, using either silver staining or pulse labeling with 35S-L-me... more Two-dimensional gel electrophoresis, using either silver staining or pulse labeling with 35S-L-methionine and autoradiography, was employed to determine changes in the synthesis of proteins that may be involved in the antiproliferative effects of recombinant alpha interferon (IFNrA) on Burkitt's lymphoma Daudi cells. IFNrA initiated and/or augmented the synthesis of at least 13 proteins that were distinct in molecular weights and isoelectric properties. Synthesis of several of these IFN-enhanced proteins was inhibited by actinomycin-D, an inhibitor of mRNA synthesis. Although IFN-induced antiproliferative effects were observed at 48 h, an increase in the synthesis of several proteins was observed as early as 3 h. The levels of these IFN-enhanced proteins in treated cells continued to increase through 24 h. At least two proteins of approximately 17 kD were observed to be synthesized in IFN-treated cells but not in control cells. Neither inhibition of synthesis of any particular protein nor post-synthetic modification of proteins in response to IFNrA was observed with these methods. The results of this study are compared and contrasted to those of several other laboratories.
Species distribution models (SDMs) are widely used in ecology and conservation. Presence-only SDM... more Species distribution models (SDMs) are widely used in ecology and conservation. Presence-only SDMs such as MaxEnt frequently use natural history collections (NHCs) as occurrence data, given their huge numbers and accessibility. NHCs are often spatially biased which may generate inaccuracies in SDMs. Here, we test how the distribution of NHCs and MaxEnt predictions relates to a spatial abundance model, based on a large plot dataset for Amazonian tree species, using inverse distance weighting (IDW). We also propose a new pipeline to deal with inconsistencies in NHCs and to limit the area of occupancy of the species. We found a significant but weak positive relationship between the distribution of NHCs and IDW for 66% of the species. The relationship between SDMs and IDW was also significant but weakly positive for 95% of the species, and sensitivity for both analyses was high. Furthermore, the pipeline removed half of the NHCs records. Presence-only SDM applications should consider th...
The heterogeneous mixture of proteins present in expressed prostatic fluid from forty-nine men ov... more The heterogeneous mixture of proteins present in expressed prostatic fluid from forty-nine men over 25 years old was analyzed on two-dimensional electrophoretic gels stained with silver. Samples were collected from men diagnosed with chronic prostatitis, urinary tract infection, benign prostatic hypertrophy (BPH), and prostatic carcinoma. Reference patterns were developed for all proteins observed in electrophoretograms. Over 1000 distinct proteins are contained in the comprehensive proteins pattern of prostatic fluid; however, only 18 proteins were common to all samples. No proteins unique to fluids from men with BPH or prostatic cancer were observed. Some proteins in the common proteins reference pattern of BPH patients were not seen in samples from men with prostatic cancer. Our preliminary results indicate that a series of proteins tentatively identified as variants of prostatic acid phosphatase appeared elevated in all BPH fluids but was absent or below limits of detection in samples from men with prostatic carcinoma. Our studies indicate that proteins secreted in prostatic fluid reflect physiological changes in the prostate, and certain changes may be useful indicators of malignant transformation.
Actas Del Congreso Iberoamericano De Las Lenguas En La Educacion Las Lenguas En La Educacion Cine Literatura Redes Y Nuevas Tecnologias 2013 Isbn 978 84 369 5528 6 Pags 241 249, 2013
Page 1. Stimulation and Promotion of Germination in Opuntia ficus-indica Seeds ♦ Mariela Altare1,... more Page 1. Stimulation and Promotion of Germination in Opuntia ficus-indica Seeds ♦ Mariela Altare1, Sinibaldo Trione1, Juan C. Guevara1, and Mariano Cony1 1Instituto Argentino de Investigaciones de las Zonas Áridas (IADIZA-CONICET) CC. 507 (5500) Mendoza, Argentina ...
In this report, we describe methods used to analyze the protein composition of sectioned frozen p... more In this report, we describe methods used to analyze the protein composition of sectioned frozen prostatic tissues by two-dimensional gel electrophoresis. Our results show a high degree of homology in twodimensional electrophoretograms of proteins extracted from frozen sec tions of malignant prostate glands. Such homology was not apparent in protein patterns of benign hypertrophie prostatic tissue sections. Typi cally, 600 discrete proteins were resolved on two-dimensional electro phoretograms and 9 proteins were present in all patterns of prostate adenocarcinomatous tissues. These nine proteins were not observed in any of the protein electrophoretograms developed from nonmalignant prostate tissue. Three proteins were found common to nonmalignant prostate glands but were not present in prostatic adenocarcinoma.
The heterogeneous mixture of proteins present in expressed prostatic fluid from forty-nine men ov... more The heterogeneous mixture of proteins present in expressed prostatic fluid from forty-nine men over 25 years old was ana lyzed on two-dimensional electrophoretic gels stained with silver. Samples were collected from men diagnosed with chronic prostatitis, urinary tract infection, benign prostatic hypertrophy (BPH), and prostatic carcinoma. Reference patterns were developed for all proteins observed in electrophoretograms. Over 1000 distinct proteins are contained in the comprehensive proteins pattern of prostatic fluid; however, only 18 proteins were common to all samples. No proteins unique to fluids from men with BPH or prostatic cancer were observed. Some proteins in the common proteins reference pattern of BPH patients were not seen in samples from men with prostatic cancer. Our preliminary results indicate that a series of proteins tentatively identified as variants of prostatic acid phosphatase appeared elevated in all BPH fluids but was absent or below limits of detection in samples from men with prostatic carcinoma. Our studies indicate that proteins secreted in prostatic fluid reflect physiological changes in the prostate, and certain changes may be useful indicators of malig nant transformation.
En este artículo se describe un sistema multiagente capaz de detectar operaciones de consulta, en... more En este artículo se describe un sistema multiagente capaz de detectar operaciones de consulta, envío y borrado de correos en un sistema de correo electrónico. El trabajo implicó el montaje y configuración del sistema de correo electrónico Exchange, la creación de un sistema multiagente -siguiendo la metodología AOPOA con agentes en los clientes y el servidor, de tal forma que permitieran detectar las operaciones de consulta-, envío y borrado de correos no autorizados, la definición de un protocolo de prueba del sistema y el análisis de los resultados, obtenidos de la aplicación del sistema multiagente. Adicionalmente, se creó un firewall que permitiera registrar las mismas operaciones de detección y comprobar el funcionamiento del sistema multiagente.
We report here new evidence of tridactyl dinosaur footprints, comprising nine isolated footprints... more We report here new evidence of tridactyl dinosaur footprints, comprising nine isolated footprints from a new locality in the Atacama Region, northern Chile. The track-bearing levels are located mainly at La Descubridora ravine and one isolated track near the intersection between La Descubridora and Carrizalillo ravines, approximately 11 km southeast from Tierra Amarilla town; the studied levels are assigned to the Pabellon Formation (Lower Cretaceous). The icnites correspond at least to two dinosaur forms, mainly ornithopods (with affinities to Hadrosauriformes dinosaurs) and theropod footprints. These are the first well-documented dinosaur icnites and the first record of ornithopod footprints from the Atacama Region. This record expands the knowledge of ornithopods in Chile, which previously were only known from the Chacarilla Formation, Tarapacá Region, the Baños del Flaco Formation in O'Higgins Region and the Dorotea Formation in Magallanes Region.
Departamento de Ingenieria Quimica, Facultad de Ciencias Fisicas y Matemiticas, Universidad de Ch... more Departamento de Ingenieria Quimica, Facultad de Ciencias Fisicas y Matemiticas, Universidad de Chile, Casilla 2777 y Centro para la Investigacih Interdisciplinaria Avanzada en Ciencias de 10s Materiales (CIMAT). Santiago, Chile. ... Departamento de Quimica Orginica y ...
Two-dimensional gel electrophoresis, using either silver staining or pulse labeling with 35S-L-me... more Two-dimensional gel electrophoresis, using either silver staining or pulse labeling with 35S-L-methionine and autoradiography, was employed to determine changes in the synthesis of proteins that may be involved in the antiproliferative effects of recombinant alpha interferon (IFNrA) on Burkitt's lymphoma Daudi cells. IFNrA initiated and/or augmented the synthesis of at least 13 proteins that were distinct in molecular weights and isoelectric properties. Synthesis of several of these IFN-enhanced proteins was inhibited by actinomycin-D, an inhibitor of mRNA synthesis. Although IFN-induced antiproliferative effects were observed at 48 h, an increase in the synthesis of several proteins was observed as early as 3 h. The levels of these IFN-enhanced proteins in treated cells continued to increase through 24 h. At least two proteins of approximately 17 kD were observed to be synthesized in IFN-treated cells but not in control cells. Neither inhibition of synthesis of any particular protein nor post-synthetic modification of proteins in response to IFNrA was observed with these methods. The results of this study are compared and contrasted to those of several other laboratories.
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