The method for extracting Triton X-100 used by I. H. Mather and C. B. Tampling [Anal. Biochem. 93... more The method for extracting Triton X-100 used by I. H. Mather and C. B. Tampling [Anal. Biochem. 93, 139-142 (1979)], has been extended to other detergents of different charge and chemical nature. All the detergents tested can be extracted with isopentanol in conditions in which not more than 8% of hydrophobic or hydrophilic protein is lost from the water phase. The removal of detergent from reaction centers and light harvesting protein-pigment complexes of photosynthetic bacteria, eliminates the artifacts of oligomers when analyzed by sodium dodecyl sulfate-gel electrophoresis.
S íncope es la pérdida súbita, transitoria y autolimitada de la conciencia, con recuperación espo... more S íncope es la pérdida súbita, transitoria y autolimitada de la conciencia, con recuperación espontánea, completa y usualmente rápida. Hay pérdida del tono postural secundaria a una inadecuada perfusión cerebral con sangre oxigenada.
The method for extracting Triton X-100 used by I. H. Mather and C. B. Tampling [Anal. Biochem. 93... more The method for extracting Triton X-100 used by I. H. Mather and C. B. Tampling [Anal. Biochem. 93, 139-142 (1979)], has been extended to other detergents of different charge and chemical nature. All the detergents tested can be extracted with isopentanol in conditions in which not more than 8% of hydrophobic or hydrophilic protein is lost from the water phase. The removal of detergent from reaction centers and light harvesting protein-pigment complexes of photosynthetic bacteria, eliminates the artifacts of oligomers when analyzed by sodium dodecyl sulfate-gel electrophoresis.
S íncope es la pérdida súbita, transitoria y autolimitada de la conciencia, con recuperación espo... more S íncope es la pérdida súbita, transitoria y autolimitada de la conciencia, con recuperación espontánea, completa y usualmente rápida. Hay pérdida del tono postural secundaria a una inadecuada perfusión cerebral con sangre oxigenada.
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