Joe-Ann McCoy

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Address: Asheville, North Carolina, United States

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Daniel D. Hutto

University of Wollongong

Galen Strawson

The University of Texas at Austin

James Elkins

School of the Art Institute of Chicago

Asha Vijayan

Amrita Vishwa Vidyapeetham

David Seamon

Kansas State University

Shaun Gallagher

University of Memphis

Kurnia Ramadhan

Bakrie School of Management

Hanaa Ahmed

Animal Health Research Institute

IJSER Journal

IJSER

Prof. Tofazzal Islam

Bangabandhu Sheikh Mujibur Rahman Agricultural University

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Papers by Joe-Ann McCoy

Comparison of Flow Injection MS, NMR, and DNA Sequencing: Methods for Identification and Authentication of Black Cohosh (Actaea racemosa)

Planta medica, Jan 21, 2015

Flow injection mass spectrometry and proton nuclear magnetic resonance spectrometry, two metaboli... more Flow injection mass spectrometry and proton nuclear magnetic resonance spectrometry, two metabolic fingerprinting methods, and DNA sequencing were used to identify and authenticate Actaea species. Initially, samples of Actaea racemosa from a single source were distinguished from other Actaea species based on principal component analysis and soft independent modeling of class analogies of flow injection mass spectrometry and proton nuclear magnetic resonance spectrometry metabolic fingerprints. The chemometric results for flow injection mass spectrometry and proton nuclear magnetic resonance spectrometry agreed well and showed similar agreement throughout the study. DNA sequencing using DNA sequence data from two independent gene regions confirmed the metabolic fingerprinting results. Differences were observed between A. racemosa samples from four different sources, although the variance within species was still significantly less than the variance between species. A model based on t...

Use of flow injection mass spectrometric fingerprinting and chemometrics for differentiation of three black cohosh species

by Joe-Ann McCoy and James Harnly

Spectrochimica Acta Part B: Atomic Spectroscopy, 2015

ABSTRACT Flow injection mass spectrometry (FIMS) was used to provide chemical fingerprints of bla... more ABSTRACT Flow injection mass spectrometry (FIMS) was used to provide chemical fingerprints of black cohosh (Actaea racemosa L.) in a manner of minutes by omitting the separation step. This method has proven to be a powerful tool for botanical authentication and in this study it was used to distinguish between three Actaea species prior to a more detailed chemical analysis using ultra high-performance liquid chromatography high-resolution mass spectrometry (UHPLC–HRMS). Black cohosh has become increasingly popular as a dietary supplement in the United States for the treatment of symptoms related to menopause. However, it has been known to be adulterated with the Asian Actaea dahurica (Turcz. ex Fisch. &amp; C.A.Mey.) Franch. species (syn. Cimicifuga dahurica (Turcz.) Maxim). Existing methods for identification of black cohosh and differentiation of Actaea species are usually lengthy, laborious, and lack robustness, often based on the comparison of a few pre-selected components. Chemical fingerprints were obtained for 77 black cohosh samples and their related species using FIMS in the negative ion mode. The analysis time for each sample was less than 2 min. All data were processed using principal component analysis (PCA). FIMS fingerprints could readily differentiate all three species. Representative samples from each of the three species were further examined using UHPLC–MS to provide detailed profiles of the chemical differences between the three species and were compared to the PCA loadings. This study demonstrates a simple, fast, and easy analytical method that can be used to differentiate A. racemosa, Actaea podocarpa, and A. dahurica.

Combining MS and NMR methods for identification and authentication of botanicals

Planta Medica, 2014

A Comprehensive Echinacea Germplasm Collection Located at the NCRPIS, Ames, IA

Joe-Ann McCoy

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