Papers by Jesus Hernandez Saavedra
Aislamiento y caracterización del gen ORF5 del virus del síndrome reproductivo y respiratorio por... more Aislamiento y caracterización del gen ORF5 del virus del síndrome reproductivo y respiratorio porcino (PRRS) en México Aislamiento y caracterización del gen ORF5 del virus del síndrome reproductivo y respiratorio porcino (PRRS) en México Isolation and characterization of the gene ORF5 of porcine reproductive and respiratory syndrome (PRRS) virus in Mexico Recibido el 7 de marzo de 2005 y aceptado el 5 de septiembre de 2005.
Virology, 2009
Delayed development of virus-specific immune response has been observed in pigs infected with the... more Delayed development of virus-specific immune response has been observed in pigs infected with the porcine reproductive and respiratory syndrome virus (PRRSV). Several studies support the hypothesis that the PRRSV is capable of modulating porcine immune system, but the mechanisms involved are yet to be defined. In this study, we evaluated the induction of T regulatory cells by PRRSV-infected dendritic cells (DCs). Our results showed that PRRSV-infected DCs significantly increased Foxp3 + CD25 + T cells, an effect that was reversible by IFN-α treatment, and this outcome was reproducible using two distinct PRRSV strains. Analysis of the expressed cytokines suggested that the induction of Foxp3 + CD25 + T cells is dependent on TGF-β but not IL-10. In addition, a significant up-regulation of Foxp3 mRNA, but not TBX21 or GATA3, was detected. Importantly, our results showed that the induced Foxp3 + CD25 + T cells were able to suppress the proliferation of PHA-stimulated PBMCs. The T cells induced by the PRRSV-infected DCs fit the Foxp3 + CD25 + T helper 3 (Th3) regulatory cell phenotype described in the literature. The induction of this cell phenotype depended, at least in part, on PRRSV viability because IFN-α treatment or virus inactivation reversed these effects. In conclusion, this data supports the hypothesis that the PRRSV succeeds to establish and replicate in porcine cells early post-infection, in part, by inducing Th3 regulatory cells as a mechanism of modulating the porcine immune system.
Research in Veterinary Science, 2008
The aim of this study was to investigate the effects of dietary supplementation of sows with α-to... more The aim of this study was to investigate the effects of dietary supplementation of sows with α-tocopherol acetate (ATA) and vitamin C on deposition of α-tocopherol (AT) in piglet lymphoid organs, such as bone marrow, thymus, and spleen at birth and at weaning, as well as on indicators of immune response in piglets. Sows were given the following treatment diets:
Veterinary Immunology and Immunopathology, 2009
Please cite this article as: Hernández, J., Soto-Canevett, E., Pinelli-Saavedra, A., Resendiz, M.... more Please cite this article as: Hernández, J., Soto-Canevett, E., Pinelli-Saavedra, A., Resendiz, M., Moya-Camarena, S.Y., Klasing, K.C., In vitro effect of vitamin E on lectin-stimulated porcine peripheral blood mononuclear cells, Veterinary Immunology and Immunopathology (2008), Abstract 17 18 In order to analyze the effect of vitamin E on Th1 and Th2 cytokine production, porcine peripheral 19 blood mononuclear cells (PBMC) were isolated from healthy pigs (n= 8) and cultured with either 0, 20 10, 50, or 100 µM of vitamin E (α-tocopherol). PBMC were stimulated with PHA for either, 24 h to 21 determine: a) the concentration of tocopherol incorporated into the cell membrane, b) cytokine 22 production and c) Th1 and Th2 regulators gene expression; or 72 h to determine the proliferation of 23 PBMC. Vitamin E was incorporated into the PBMC in a dose dependent manner, giving as a result 24 a high proliferation of cells irrespective of the dose of vitamin E used. Regarding cytokine 25 production, vitamin E consistently decreases the mRNA expression and the percentage of cells 26
British Journal of Nutrition, 2008
Micronutrient deficiencies are frequently associated with tuberculosis (TB) worldwide. We tested ... more Micronutrient deficiencies are frequently associated with tuberculosis (TB) worldwide. We tested the effect of exogenous vitamin E on proliferation and cytokine production of peripheral blood mononuclear cells (PBMC) from TB patients and healthy purified protein derivative (PPD) þ volunteers. Proliferation was stimulated with mycobacterial antigen (PPD) and evaluated by the incorporation of tritiated thymidine in PBMC cultured with or without 50 mM-vitamin E for 6 d. Cytokine production (IL-2 and interferon (IFN)-g) was determined by intracellular cytokine staining and by ELISA in the supernatant of PBMC stimulated for 24 h with phytohaemagglutinin or PPD. Our results show that culture with vitamin E increased (P#0·05) the antigen-induced proliferation of PBMC in TB patients but not in healthy PPD þ volunteers. No significant changes in the number of cytokine-producing cells or in the production of IFN-g were observed with vitamin E treatment. These results indicate that vitamin E may enhance the antigen-specific in vitro response of PBMC from TB patients. Tuberculosis: Vitamin E: Purified protein derivative * Corresponding author: Dr Jesús Hernández, fax þ52 662 280 0094, email [email protected] Abbreviations: IFN, interferon; PBMC, peripheral blood mononuclear cell; PPD, purified protein derivative; PHA, phytohaemagglutinin; TB, tuberculosis.
Revista Espanola De Cardiologia, 2008
Revista Espanola De Cardiologia, 2008
Clinical Cardiology, 2010
Primary antiphospholipid syndrome (PAPS) is characterized by arterial and venous thrombosis, recu... more Primary antiphospholipid syndrome (PAPS) is characterized by arterial and venous thrombosis, recurrent spontaneous abortion, and thrombocytopenia. The arterial thromboses affect the brain in most cases. Coronary thrombosis can be observed in 25% of patients; however, myocardial infarction is very unusual as the first manifestation of PAPS. We report the case of a young man with cigarette smoking as only the risk factor for coronary artery disease who presented with a single episode of chest pain. The echocardiogram showed severe left ventricular dysfunction. Coronary angiography revealed complete occlusion of the proximal left anterior descending and the proximal circumflex coronary arteries. Laboratory study demonstrated prolonged activated partial thromboplastin time and high titers of anticardiolipin antibodies. Rheumatologic diseases were excluded, so the diagnosis of PAPS was established. With oral anticoagulation, after 20 mo of follow-up no new thrombotic event has been recorded.
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Papers by Jesus Hernandez Saavedra