Atomic force microscopy has been used previously to study the morphology of Streptococcus mutans ... more Atomic force microscopy has been used previously to study the morphology of Streptococcus mutans bacteria and dehydrated biofilms. Objectives: To characterize the topographies of hydrated S. mutans biofilms during the first three hours of formation on resin-composite. Methods: Specimens of Point 4 microhybrid resin-composite (Kerr Corp.; d=6.0mm, h=0.3mm) were sequentially wet-polished (MultiPrep grinder-polisher, Allied High Tech; 180 through 1200grit SiC disks), sterilized (ethylene oxide) and divided into two groups (n=9/group). Streptococcus mutans (UA159) biofilms were grown on specimens in 0.5x Tryptone-Yeast extract medium supplemented with 0.17% sucrose for 1, 2, and 3 hours, then washed with sterile, ultrapure water to constitute the Biofilm group. Control group specimens were subjected to the same growth protocol but without bacterial innoculum. Specimens were scanned at 37C in PBS using a MultiMode-AFM/NanoScope-V controller (tapping-mode; 100x100m scans @0.25Hz; NP-S pro...
Atomic force microscopy has been used previously to study the morphology of Streptococcus mutans ... more Atomic force microscopy has been used previously to study the morphology of Streptococcus mutans bacteria and dehydrated biofilms. Objectives: To characterize the topographies of hydrated S. mutans biofilms during the first three hours of formation on resin-composite. Methods: Specimens of Point 4 microhybrid resin-composite (Kerr Corp.; d=6.0mm, h=0.3mm) were sequentially wet-polished (MultiPrep grinder-polisher, Allied High Tech; 180 through 1200grit SiC disks), sterilized (ethylene oxide) and divided into two groups (n=9/group). Streptococcus mutans (UA159) biofilms were grown on specimens in 0.5x Tryptone-Yeast extract medium supplemented with 0.17% sucrose for 1, 2, and 3 hours, then washed with sterile, ultrapure water to constitute the Biofilm group. Control group specimens were subjected to the same growth protocol but without bacterial innoculum. Specimens were scanned at 37C in PBS using a MultiMode-AFM/NanoScope-V controller (tapping-mode; 100x100m scans @0.25Hz; NP-S pro...
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