Papers by Jadwiga Dudkiewicz Wilczyńska
Acta Poloniae Pharmaceutica - Drug Research
A series of eight non-peptidic potential renin inhibitors have been designed and synthesized. All... more A series of eight non-peptidic potential renin inhibitors have been designed and synthesized. All of them contain dipeptide replacement: (3S,4S)-4-amino-5-cyclohexyl-3-hydroxypentanoic acid (ACHPA) in their molecules. Four among them comprise two additional analogs of dipeptide: (3S,4S)-4-amino-3-hydroxy-5-phenylpentanoic acid (AHPPA) and (3S,4S)-4-amino-3-hydroxy-6-methylheptanoic acid (statine, Sta). All of the synthesized compounds contain also hydrophobic portions to receive a moderate lipophilicity of the molecules. Inhibitory activity of the compounds was measured in vitro by I-IPLC determination of Leu-Val-Tyr-Ser released from the N-acetyltetradecapeptide substrate by renin in the presence of the inhibitor. Asp-alpha(OEt)-(S,S)-ACHPA-epsilonAhx-Iaa (23) shows inhibitory activity (7%) at the concentration of 1.0 x 10(-2) M. The other synthesized compounds show no inhibitory activity up to this concentration.
Acta Poloniae Pharmaceutica - Drug Research
The RP-HPLC method, useful for the separation of a protein of the erythropoietin formulations wit... more The RP-HPLC method, useful for the separation of a protein of the erythropoietin formulations with different stabilizers, was elaborated. Using this method, rhEPO, HSA and other proteins, can be determined, depending on the sample composition. The method is sensitive and rapid. Our results indicate that this method is useful for quality control of rhEPO in final drug preparations with albumin as stabilizer. The method was also capable of distinguishing products from different manufacturers.
Acta poloniae pharmaceutica, 2004
A high-performance liquid chromatographic assay has been developed to the separation of angiotens... more A high-performance liquid chromatographic assay has been developed to the separation of angiotensin I, tetradecapeptide and the tetrapeptide Leu-Val-Tyr-Ser. This purpose is achieved in a single step, using HPLC technique in the reversed phase system. The method is based on enzymatic hydrolysis of a substrate renin (TDP) with formation of angiotensin I (DP). After 1 h incubation at 37 degrees C the reaction mixture is introduced directly into the chromatographic system. A 200 microl reaction mixture is needed for analysis. Acetonitrile gradient in 0.01 M ammonium acetate buffer allows a satisfactory separation of hydrolysis products. The application of this technique for the determination of in vitro renin activity and evaluation the potency of human renin inhibitors, employing the artificial renin substrate tetradecapeptide, is demonstrated.
Acta poloniae pharmaceutica, 2002
High performance size exclusion chromatography with fluorimetric detection for the determination ... more High performance size exclusion chromatography with fluorimetric detection for the determination of erythropoietin in pharmaceutical preparations has been developed. The applied chromatographic system has been enabled a quick estimation of the identity and contents of the studied compound. The method was validated and showed good validation data in terms of linearity, precision and repeatability. The validated method was successfully applied to the determination of erythropoietin content in commercially available preparations.
The aim of this study was to apply high performance liquid chromatography to the determination of... more The aim of this study was to apply high performance liquid chromatography to the determination of content of desmopressin in pharmaceutical preparations and validation of the method. The satisfactory results have been obtained using a column Luna C 8.5 microm, 100 x 4.6 mm and a mobile phase containing 0.067 M phosphate buffer of pH = 7 and acetonitrile in the proportion 83:17. It has been shown that the elaborated method shows good precision and accuracy and can be applied to the qualitative and quantitative analysis of pharmaceutical preparations containing desmopressin.
Journal of Pharmaceutical and Biomedical Analysis, 1998
Journal of Pharmaceutical and Biomedical Analysis, 2004
Benzalkonium chloride (BAC) is a bacteriostatic agent used in the pharmaceutical industry as a pr... more Benzalkonium chloride (BAC) is a bacteriostatic agent used in the pharmaceutical industry as a preservative. BAC is a mixture of alkylbenzyldimethylammonium chlorides, the three most important of which being those with alkyl substituents C12, C14, C16 at the quaternary ammonium salt. The purpose of this study was to develop a method for determining benzalkonium chloride identity and content in aerosol preparations in which protein or steroid hormones are the active components. The high performance liquid chromatography (HPLC) method was used for this purpose. In the performed comparison of the influence of selected factors on the process of the separation of BAC homologues, a column with packing modified with cyan groups and mobile phase containing 0.075 M acetate buffer with acetonitrile (45:55), in an isocratic elution, was used for qualitative and quantitative determinations and for method validation. The developed method may be used for the assessment of the identity and content of BAC homologues in various pharmaceutical preparations. It is simple and it does not require particular sample preparation for the tests. It is characterized by good selectivity and high precision of the determinations.
Fibers and Polymers, 2013
PLA fibrous mats containing nanoalumina filler were fabricated by electrospinning method. The mor... more PLA fibrous mats containing nanoalumina filler were fabricated by electrospinning method. The morphology of the mats was characterized by SEM, and TEM. In vitro biocompatibility of the electrospun fiber mats was also evaluated. Indirect cytotoxicity evaluation of the fiber mats with human skin fibroblasts revealed that the materials were non-toxic to living cells. The cells cultured on the fibrous mat exhibited normal cells shapes and were integrated well with surrounding fibers. The obtained results confirmed the potential for use of the electrosupun PLA/Al 2 O 3 fiber mats for biomedical application.
CHEMICAL & PHARMACEUTICAL BULLETIN, 2005
A series of four non-peptidic renin inhibitors have been designed and synthesized. All of them co... more A series of four non-peptidic renin inhibitors have been designed and synthesized. All of them contain in their molecule (3S,4S)-4-amino-5-cyclohexyl-3-hydroxypentanoic acid (ACHPA), a hydrophobic portion at the Cterminus and a second dipeptide-like transition state analog or unnatural dipeptidic fragment at the N-terminus. Inhibitory activity of the compounds was measured in vitro by high performance liquid chromatography (HPLC). Their IC 50 (M/l) values were: Ͻ10 ؊3 (12), 1.0؋10 ؊6 (19), 4.0؋10 ؊4 (23) and 1.0؋10 ؊6 (29), respectively. All the compounds are stable against chymotrypsin.
Advances in Applied Ceramics, 2011
ABSTRACT The present paper describes an In vitro assessment of the antibacterial properties and c... more ABSTRACT The present paper describes an In vitro assessment of the antibacterial properties and cytotoxic activity on mammalian cells of silver nanoparticles incorporated into an aluminium nano-oxide substrate produced by the thermal decomposition-reduction method. The Al2O3-Ag nanopowders show good bactericidal and fungicidal properties. An Al2O3-Ag (12·55 wt-%) specimen at the concentration of 50 mg mL-1 reduced considerably the populations of Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus and Bacillus subtilis species, whereas the same nanopowder in a concentration of 90 mg mL-1 inhibited significantly the growth of P. aeruginosa, E. coli, S. aureus and Candida albicans and also significantly limited the number of B. subtilis. The Al2O3-Ag nanoparticles also showed no cytotoxic effects on the three selected cell lines, i.e. L929, BJ and HeLa, and the cells remained >75% viability, as measured by MTT assay, and >102% viability, obtained by EZ4U assay, relative to control at concentration as high as 200 mg mL-1.
Acta poloniae pharmaceutica
Conditions have been established for the determination of oxytocin by the HPLC method; the method... more Conditions have been established for the determination of oxytocin by the HPLC method; the method has been validated. The results of HPLC determinations are compared with those obtained by the biological method.
Acta poloniae pharmaceutica, 2010
A series of eight non-peptidic potential renin inhibitors have been designed and synthesized. All... more A series of eight non-peptidic potential renin inhibitors have been designed and synthesized. All of them contain dipeptide replacement: (3S,4S)-4-amino-5-cyclohexyl-3-hydroxypentanoic acid (ACHPA) in their molecules. Four among them comprise two additional analogs of dipeptide: (3S,4S)-4-amino-3-hydroxy-5-phenylpentanoic acid (AHPPA) and (3S,4S)-4-amino-3-hydroxy-6-methylheptanoic acid (statine, Sta). All of the synthesized compounds contain also hydrophobic portions to receive a moderate lipophilicity of the molecules. Inhibitory activity of the compounds was measured in vitro by I-IPLC determination of Leu-Val-Tyr-Ser released from the N-acetyltetradecapeptide substrate by renin in the presence of the inhibitor. Asp-alpha(OEt)-(S,S)-ACHPA-epsilonAhx-Iaa (23) shows inhibitory activity (7%) at the concentration of 1.0 x 10(-2) M. The other synthesized compounds show no inhibitory activity up to this concentration.
Acta poloniae pharmaceutica, 2005
The RP-HPLC method, useful for the separation of a protein of the erythropoietin formulations wit... more The RP-HPLC method, useful for the separation of a protein of the erythropoietin formulations with different stabilizers, was elaborated. Using this method, rhEPO, HSA and other proteins, can be determined, depending on the sample composition. The method is sensitive and rapid. Our results indicate that this method is useful for quality control of rhEPO in final drug preparations with albumin as stabilizer. The method was also capable of distinguishing products from different manufacturers.
International Journal of Applied Ceramic Technology
Acta poloniae pharmaceutica
High performance size exclusion chromatography with fluorimetric detection for the determination ... more High performance size exclusion chromatography with fluorimetric detection for the determination of erythropoietin in pharmaceutical preparations has been developed. The applied chromatographic system has been enabled a quick estimation of the identity and contents of the studied compound. The method was validated and showed good validation data in terms of linearity, precision and repeatability. The validated method was successfully applied to the determination of erythropoietin content in commercially available preparations.
Acta poloniae pharmaceutica
The RP-HPLC method, useful for the separation of a protein of the erythropoietin formulations wit... more The RP-HPLC method, useful for the separation of a protein of the erythropoietin formulations with different stabilizers, was elaborated. Using this method, rhEPO, HSA and other proteins, can be determined, depending on the sample composition. The method is sensitive and rapid. Our results indicate that this method is useful for quality control of rhEPO in final drug preparations with albumin as stabilizer. The method was also capable of distinguishing products from different manufacturers.
Acta poloniae pharmaceutica
A series of eight non-peptidic potential renin inhibitors have been designed and synthesized. All... more A series of eight non-peptidic potential renin inhibitors have been designed and synthesized. All of them contain dipeptide replacement: (3S,4S)-4-amino-5-cyclohexyl-3-hydroxypentanoic acid (ACHPA) in their molecules. Four among them comprise two additional analogs of dipeptide: (3S,4S)-4-amino-3-hydroxy-5-phenylpentanoic acid (AHPPA) and (3S,4S)-4-amino-3-hydroxy-6-methylheptanoic acid (statine, Sta). All of the synthesized compounds contain also hydrophobic portions to receive a moderate lipophilicity of the molecules. Inhibitory activity of the compounds was measured in vitro by I-IPLC determination of Leu-Val-Tyr-Ser released from the N-acetyltetradecapeptide substrate by renin in the presence of the inhibitor. Asp-alpha(OEt)-(S,S)-ACHPA-epsilonAhx-Iaa (23) shows inhibitory activity (7%) at the concentration of 1.0 x 10(-2) M. The other synthesized compounds show no inhibitory activity up to this concentration.
Cancer genomics & proteomics
Selol is a mixture of selenitriglycerides, obtained by the chemical modification of sunflower oil... more Selol is a mixture of selenitriglycerides, obtained by the chemical modification of sunflower oil, which contain selenium at the +4 oxidation state. The aim of the present study was to describe the changes in the expression of genes related to oxidative stress caused by Selol in prostate cells: both normal (PNT1A) and malignant (LNCaP). The changes in gene expression in PNT1A and LNCaP cell lines under the influence of Selol were measured using a 96-well RT(2) Profiler ™PCR Array: Human Oxidative Stress and Antioxidant Defense, which arrayed 84 genes functionally involved in the cellular oxidative stress response. Based on the obtained data, LNCaP cells exhibited a significantly lower potential for antioxidant defence when compared to PNT1A cells. The response of the malignant LNCaP cells to exposure to Selol was significantly different from that of the normal PNT1A cells, especially after 48 h of incubation. In the case of LNCaP cells, Selol causes down-regulation of the expression...
Współczesna Onkologia, 2014
Aim of the study: Cellular resistance is strongly correlated with the risk of failure in doxorubi... more Aim of the study: Cellular resistance is strongly correlated with the risk of failure in doxorubicin (DOX) treatment, and the knowledge of the mechanisms of resistance and its possible modulation is still very limited. Material and methods: In this study, we assessed the effect of 5% Selol and DOX on the expression of genes that affect cell proliferation in the resistant KB-V1 and sensitive HeLa cell lines, using RT2 ProfilerTM PCR Array matrix "Human Cancer Drug Resistance and Metabolism" (SABiosciences). Results: We showed that HeLa and KB-V1 cell lines, characterised by varying susceptibility to DOX, have different genetic profiles as regards the studied genes. KB-V1 cells show overexpression of MYC and BCL2 genes, which encode proteins with anti apoptotic properties. Selol, when used in KB-V1 cells, reduced the expression of MYC and BCL2 genes, suggested as a new therapeutic target in the treatment of cancers resistant to cytostatic drugs. Conclusions: The results suggest that Selol could be used as a modulator that enhances the cytotoxic effects of doxorubicin, particularly in cells resistant to this drug.
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Papers by Jadwiga Dudkiewicz Wilczyńska