Papers by J. Arellano-galindo
We used nested multiplex polymerase chain reaction to determine the distribution of cytomegalovir... more We used nested multiplex polymerase chain reaction to determine the distribution of cytomegalovirus gB genotype in 30 Mexican children undergoing bone marrow transplantation. The genotypes were: gB1, 9/30 (30%); gB2, 8/30 (27%); gB3, 4/30 (13%), and gB4, 1/30 (3%); mixed genotypes were identified in 8/30 patients (27%). We conclude that the predominant genotypes identified were gB2 and gB1. The gB5 genotype was not detected, and a high proportion of mixed genotype was found.
Background. Autologous bone marrow transplantation (ABMT) after myeloablative therapy is an alter... more Background. Autologous bone marrow transplantation (ABMT) after myeloablative therapy is an alternative treatment for patients with malignant diseases who lack suitable donors. The rationale for marrow purging is based on differential sensitivity to chemotherapy between leukemic and normal stem cells. Methods. ABMT procedure was performed by in vitro marrow purging with mafosfamide in patients diagnosed with acute myeloid leukemia (AML) and acute lymphoblastic leukemia Philadelphia chromosome positive (ALL Ph+) in first remission, who do not have HLA identical donor. Eight patients (median age 9 years): four with AML, three with ALL Ph+ and a patient with ALL in fourth complete remission, received Vázquez-Meraz E*, Montaño-Figueroa EH**, Mendoza-García E**, Arellano-Galindo J***, Rosas-Cabral A****.
Clinical Lymphoma Myeloma and Leukemia, 2013
The frequency of Epstein-Barr virus (EBV) and the distribution of EBNA genotypes in children and ... more The frequency of Epstein-Barr virus (EBV) and the distribution of EBNA genotypes in children and adults with Hodgkin lymphoma (HL) in Mexico were analyzed. The frequency of EBV infection in patients previously diagnosed with lymphoma was high, was found most frequently in children rather than adults, and was more frequent in male patients than in female patients. EBNA2B was the most frequent genotype. Background: Epstein-Barr virus (EBV) is a member of the Herpesviridae family and is associated with Hodgkin lymphoma (HL). Isolates of EBV are classified according to sequence variation in the latency genes such as Epstein-Barr virus nuclear antigen (EBNA). EBNA2 contains the most divergent locus and is classified into type 1 and type 2 or EBNA2A and EBNA2B, respectively. We compared the frequency of EBV and the distribution of EBNA genotypes in Mexican children and adults with HL. Patients and Methods: Lymph node biopsy specimens from children and adults with HL were embedded in paraffin. EBV was identified by LMP1 amplification and Epstein-Barrencoded RNA EBER by in situ hybridization (ISH) and genotyped as EBNA2A or EBNA2B using nested polymerase chain reaction (PCR) and specific primers for the detection of subtype. Results: Sixty-six samples were obtained from 3 hospitals-42 (63%) from children and 24 (37%) from adults with HL. Thirty-two of the 42 samples (76.1%) were positive for EBV in children and 16 of 24 (66.6%) samples were positive in adults (P ϭ .41). In both children and adults, Drs Palma and Sanchez contributed equally to this work.
Clinical laboratory
Background: Adenovirus (AdV) causes respiratory infection; recent observations suggest that some ... more Background: Adenovirus (AdV) causes respiratory infection; recent observations suggest that some subtypes have more ability to develop fatal disease. AdV infection has been associated with co-infection with human bocavirus (HBoV). We analysed the frequency of AdV infection, its subtypes and the presence of co-infection with HBoV, as well the clinical characteristics of such co-infection in Mexican paediatric immunosuppressed (IP) and non-immunosuppressed patients (non-IP) diagnosed with pneumonia. Methods: A total of 5185 nasopharyngeal swabs from two groups of children with pneumonia, one IP and the other non-IP, were analysed for the detection of AdV by immunofluorescence and confirmed by PCR and culture. HBoV was identified by PCR. Positive samples for AdV and AdV/HBoV were typed using PCR sequencing, the clinical characteristics of the AdV/HBoV co-infection were analysed. Results: Thirty-seven of the 5185 (0.71%) samples were positive for AdV, of those 27/37 (73%) were detected ...
Clinical and investigative medicine. Médecine clinique et experimentale, 2007
To evaluate the hemolysin effect by ileal loop model produced by Vibrio cholerae O1 strains, comp... more To evaluate the hemolysin effect by ileal loop model produced by Vibrio cholerae O1 strains, compared with the cellular lysis or cytotoxic activity (CA) observed in cell culture. We studied nine V. cholerae O1 strains, obtained during the Mexican outbreak of cholera (1990-1993), which had CA in Vero and CHO cells. Hemolysin was monitored with the hemolysis test. Titers of CA were calculated by CD50, and the association between CA and cholera toxin (CT) production was discarded by means of neutralization tests using an anti-CT polyclonal antibody. The CT production was measured with ELISA test. The LAL assay was performed in order to study relationships between the CA and bacterial lipopolysaccharide. Strains with CA were evaluated in rabbit and rat ileal loop models; hemorrhagic fluid was also measured. Tissues from ileal loop were included in paraffin to detect intestinal epithelial damage. The hemolysin CA was not neutralized with the anti-CT polyclonal antibody. However, the asso...
Infection, Genetics and Evolution, 2015
Helicobacter pylori cagA babA2 vacA EPIYA Children a b s t r a c t Genotypic differences in Helic... more Helicobacter pylori cagA babA2 vacA EPIYA Children a b s t r a c t Genotypic differences in Helicobacter pylori play an important role in infection. We characterized the diversity of the cagA, cagE, babA2, and vacA genes in H. pylori strains isolated from pediatric patients and the relationship between these genes and clinical disease. Additionally, we employed the Neighbor-net algorithm to predict the behavior of the genotypes of the strains isolated from patients. Of 93 patients analyzed, 32 were positive for infection. A total of 160 H. pylori strains (five isolates per positive patient) were analyzed. A total of 91% and 83% of strains possessed the cagA and cagE genes, respectively. For the vacA gene, 84% of strains possessed the s1 allele, 15% the s2 allele, 81% the m1 allele and 13.8% the m2 allele. The babA2 gene was present in 79% of strains. Infection with H. pylori strains with the vacA (s1m1) genotype was associated with risk of esophagitis and gastritis (p = 0.0001). The combination of cagA and vacA (s1m1) was significantly associated with abdominal pain (p = 0.002); however, EPIYA type was not significantly associated with abdominal pain. A total of 16 different genotypes were identified; the most common genotype was vacAs1m1cagA+cagE+babA2+ (47.5%). A total of 84% of pediatric patients were infected by at least two and up to five different genotypes. The network recovered two genotype groups (A: strains with vacAs1 and B: strains with vacAs2). The presence of multiple paths in the network suggests that reticulate events, such as recombination or reinfection, have contributed to the observed genotypic diversity.
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Papers by J. Arellano-galindo