Dengue fever also known as breakbone fever is a mosquito-borne tropical disease caused by the den... more Dengue fever also known as breakbone fever is a mosquito-borne tropical disease caused by the dengue virus. Symptoms include fever, headache, muscle and joint pains, and a characteristic skin rash that is similar to measles. In a small proportion of cases, the disease develops into the life-threatening dengue hemorrhagic fever, resulting in bleeding, low levels of blood platelets and blood plasma leakage, or into dengue shock syndrome, where dangerously low blood pressure occurs. Dengue is transmitted by several species of mosquito within the genus Aedes, principally A. aegypti.
The best approach for the development of a vaccine against a specific infection or a microbial di... more The best approach for the development of a vaccine against a specific infection or a microbial disease is Insilico vaccine designing that is not only accurate but also requires minimum time, labour and economy. This approach can also be used to analyze the target structures for possible binding/active sites, generate candidate molecules, check for their drug likeness, dock these molecules with the target, rank them according to their binding affinities, further optimize the molecules to improve binding characteristics. During vaccine designing the disease to be selected should be a viral or a bacterial disease but not due to a protozoan, as they cannot be attenuated and injected. The main goal of the current work is to develop a vaccine against the disease Ebola. Computational tools offer the advantage of delivering new drug candidates more quickly and at a lower cost. This review provides succinct overview of several methods in drug discovery by using insilico approaches on identifying drug targets where there are genes or proteins associated with specific diseases.
Toxins have evolved in plants, animals and microbes multiple times as part of defensive and/or pr... more Toxins have evolved in plants, animals and microbes multiple times as part of defensive and/or prey capture strategies. Peptide toxins are usually found in animal venom. Most venom comprises a highly complex mixture of peptides, often with diverse and selective pharmacology. The source of peptide is specifically snake venom which is usually neurotoxins. We have selected four peptides Aggretin, Crotamine, Mambalgin, Myotoxin. In-silico drug design is crucial step to reduce the number of steps in drug designing process. The present study was focused to design a drug for snake venom peptides. The peptides are causative proteins for diseases like blood clotting, necrosis, acute renal failure, and anaphylaxis. The peptide structure was retrieved from Uniprot database and multiple sequence alignment was performed on the retrieved sequences using Clustal W. Rasmol software was used for protein visualization. Pubchem database was used for screening the chemical ligands. The chemical structure of selected ligands was obtained from Dundee pro drg server. The ligands were screened based on log P value for selection of better ligands which was used for docking studies. Docking was performed to find the inhibition activity against snake venom using HEX software. Autodocking was done using vina for different conformations of ligand.
In-silico drug design is crucial step to reduce the number of steps in drug designing process. Th... more In-silico drug design is crucial step to reduce the number of steps in drug designing process. The present study was focused to design a drug for snake venom peptides. We have selected a peptide Aggretin from the species Calloselasma rhodostoma, commonly known as Malayan pit-viper. The peptide structure was retrieved from Uniprot database and multiple sequence alignment was performed on the retrieved sequences using Clustal W. Rasmol software was used for protein visualization. Pubchem database was used for screening the chemical ligands. The chemical structure of selected ligands was obtained from Dundee pro drg server. The ligands were screened based on log P value for selection of better ligands which was used for docking studies. Docking was performed to find the inhibition activity against snake venom using HEX software. Highest negativity of E-value was shown by Aggretin peptide with Ellagic Acid ligand -232.51. Vibrational analysis was performed to check the energy changes and it was found that the energy of docking in case of Hex after vibrational analysis was higher.
A cost effective method for the production of cellulase by using home waste like watermelon peels... more A cost effective method for the production of cellulase by using home waste like watermelon peels/rind through solid state fermentation. the microorganism used was a soil isolate. Further effect of different temperature and pH is observed for the enzyme.
Medicinal plants prove to be very useful in reducing the adverse effects of various chemotherapeu... more Medicinal plants prove to be very useful in reducing the adverse effects of various chemotherapeutic agents as well as in prolonging longevity and achieving positive health care system The research for new therapeutic treatments for various disease conditions is expanding. In many poor countries, plants have been looked at as a very promising source of new lead compounds for drug discovery and development. Catharanthus roseus and Tinospora cordifolia are important medicinal plant. It is cultivated mainly for its alkaloids, which are having anticancer activities, diabetes, high cholesterol, rheumatoid arthritis, fever and to boost the immune system. Antibacterial potential in crude extracts of different parts of C. roseus and T. cordifolia against clinically significant bacterial strains to identify the qualitative and quantitative methods to identify the antibacterial activity of these herbs
Dengue fever also known as breakbone fever is a mosquito-borne tropical disease caused by the den... more Dengue fever also known as breakbone fever is a mosquito-borne tropical disease caused by the dengue virus. Symptoms include fever, headache, muscle and joint pains, and a characteristic skin rash that is similar to measles. In a small proportion of cases, the disease develops into the life-threatening dengue hemorrhagic fever, resulting in bleeding, low levels of blood platelets and blood plasma leakage, or into dengue shock syndrome, where dangerously low blood pressure occurs. Dengue is transmitted by several species of mosquito within the genus Aedes, principally A. aegypti.
The best approach for the development of a vaccine against a specific infection or a microbial di... more The best approach for the development of a vaccine against a specific infection or a microbial disease is Insilico vaccine designing that is not only accurate but also requires minimum time, labour and economy. This approach can also be used to analyze the target structures for possible binding/active sites, generate candidate molecules, check for their drug likeness, dock these molecules with the target, rank them according to their binding affinities, further optimize the molecules to improve binding characteristics. During vaccine designing the disease to be selected should be a viral or a bacterial disease but not due to a protozoan, as they cannot be attenuated and injected. The main goal of the current work is to develop a vaccine against the disease Ebola. Computational tools offer the advantage of delivering new drug candidates more quickly and at a lower cost. This review provides succinct overview of several methods in drug discovery by using insilico approaches on identifying drug targets where there are genes or proteins associated with specific diseases.
Toxins have evolved in plants, animals and microbes multiple times as part of defensive and/or pr... more Toxins have evolved in plants, animals and microbes multiple times as part of defensive and/or prey capture strategies. Peptide toxins are usually found in animal venom. Most venom comprises a highly complex mixture of peptides, often with diverse and selective pharmacology. The source of peptide is specifically snake venom which is usually neurotoxins. We have selected four peptides Aggretin, Crotamine, Mambalgin, Myotoxin. In-silico drug design is crucial step to reduce the number of steps in drug designing process. The present study was focused to design a drug for snake venom peptides. The peptides are causative proteins for diseases like blood clotting, necrosis, acute renal failure, and anaphylaxis. The peptide structure was retrieved from Uniprot database and multiple sequence alignment was performed on the retrieved sequences using Clustal W. Rasmol software was used for protein visualization. Pubchem database was used for screening the chemical ligands. The chemical structure of selected ligands was obtained from Dundee pro drg server. The ligands were screened based on log P value for selection of better ligands which was used for docking studies. Docking was performed to find the inhibition activity against snake venom using HEX software. Autodocking was done using vina for different conformations of ligand.
In-silico drug design is crucial step to reduce the number of steps in drug designing process. Th... more In-silico drug design is crucial step to reduce the number of steps in drug designing process. The present study was focused to design a drug for snake venom peptides. We have selected a peptide Aggretin from the species Calloselasma rhodostoma, commonly known as Malayan pit-viper. The peptide structure was retrieved from Uniprot database and multiple sequence alignment was performed on the retrieved sequences using Clustal W. Rasmol software was used for protein visualization. Pubchem database was used for screening the chemical ligands. The chemical structure of selected ligands was obtained from Dundee pro drg server. The ligands were screened based on log P value for selection of better ligands which was used for docking studies. Docking was performed to find the inhibition activity against snake venom using HEX software. Highest negativity of E-value was shown by Aggretin peptide with Ellagic Acid ligand -232.51. Vibrational analysis was performed to check the energy changes and it was found that the energy of docking in case of Hex after vibrational analysis was higher.
A cost effective method for the production of cellulase by using home waste like watermelon peels... more A cost effective method for the production of cellulase by using home waste like watermelon peels/rind through solid state fermentation. the microorganism used was a soil isolate. Further effect of different temperature and pH is observed for the enzyme.
Medicinal plants prove to be very useful in reducing the adverse effects of various chemotherapeu... more Medicinal plants prove to be very useful in reducing the adverse effects of various chemotherapeutic agents as well as in prolonging longevity and achieving positive health care system The research for new therapeutic treatments for various disease conditions is expanding. In many poor countries, plants have been looked at as a very promising source of new lead compounds for drug discovery and development. Catharanthus roseus and Tinospora cordifolia are important medicinal plant. It is cultivated mainly for its alkaloids, which are having anticancer activities, diabetes, high cholesterol, rheumatoid arthritis, fever and to boost the immune system. Antibacterial potential in crude extracts of different parts of C. roseus and T. cordifolia against clinically significant bacterial strains to identify the qualitative and quantitative methods to identify the antibacterial activity of these herbs
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Papers by Ambika G Iyer