Kalueff AV. The effects of chronic social defeat stress on mouse grooming behavior and its patter... more Kalueff AV. The effects of chronic social defeat stress on mouse grooming behavior and its patterning. Behavioural Brain
Analysis of habituation is widely used to characterize animal cognitive phenotypes and their modu... more Analysis of habituation is widely used to characterize animal cognitive phenotypes and their modulation. Although zebrafish (Danio rerio) are increasingly utilized in neurobehavioral research, their habituation responses have not been extensively investigated. Utilizing the novel tank test, we examine intra-and inter-session habituation and demonstrate robust habituation responses in adult zebrafish.
As the rate of biomedical discovery is rising exponentially, electronic databases have become par... more As the rate of biomedical discovery is rising exponentially, electronic databases have become particularly effective in organizing and sharing scienti fi c knowledge. Due to a well-characterized genome, robust behavioral responses and physiological similarity to humans, the zebra fi sh ( Danio rerio ) has emerged as a useful species for neurobehavioral research. The growing utility of this model organism requires the development of specialized databases of zebra fi sh neurophenotypes, such as the Zebra fi sh Neurophenome Project (ZNP) ( http://www.tulane.edu/~znpindex/search ). Representing a new bioinformatics-based tool, the ZNP interactive searchable database consolidates neurobehavioral and related physiological phenotypes obtained in various zebra fi sh models and tests. This chapter outlines the contribution of the ZNP to increased accessibility of current zebra fi sh neurobiological knowledge, and discusses how this database may be used for various research projects.
Zebra fi sh are a popular model organism in neuroscience research, recently emerging as an excell... more Zebra fi sh are a popular model organism in neuroscience research, recently emerging as an excellent species to study complex social phenotypes. For example, zebra fi sh actively form shoals, which can be used to quantify their shoaling behaviors. Zebra fi sh also display strong social preference when placed in a tank with conspeci fi c fi sh, a trait that can easily be quanti fi ed in the two-compartment preference test. The mirror biting test, based on mirror image stimulation, is another well-established method for studying zebra fi sh boldness and sociability. This chapter will describe three simple and ef fi cient paradigms-shoaling, social preference, and mirror biting tests-for quantifying social behaviors in adult zebra fi sh. Re fl ecting different aspects of zebra fi sh social phenotypes, these models can be used individually or within a test battery.
Assessing Habituation Phenotypes in Adult Zebrafish: Intra- and Inter-Trial Habituation in the Novel Tank Test
Neuromethods, 2012
Abstract Although adult zebrafish are increasingly utilized as a model organism in neurobehaviora... more Abstract Although adult zebrafish are increasingly utilized as a model organism in neurobehavioral research, their habituation responses have only recently been evaluated in detail. When exposed to a novel environment, zebrafish demonstrate marked habituation ...
Zebra fi sh is rapidly becoming a popular model species for neurobehavioral and psychopharmacolog... more Zebra fi sh is rapidly becoming a popular model species for neurobehavioral and psychopharmacological research. The startle response represents the instinctive, evolutionarily conserved reaction of an organism to novel unexpected and/or aversive stimuli. While startle testing is a well-established assay to study anxietylike behaviors in different species, screening of the startle response and its habituation in zebra fi sh is also an important direction of translational biomedical research. Complementing rich literature on zebra fi sh startle, this chapter outlines a brief and simple protocol to assess the tapping-induced startle response and its inter-and intra-trial habituation in adult zebra fi sh.
Analysis of habituation is widely used to characterize animal cognitive phenotypes and their modu... more Analysis of habituation is widely used to characterize animal cognitive phenotypes and their modulation. Although zebrafish (Danio rerio) are increasingly utilized in neurobehavioral research, their habituation responses have not been extensively investigated. Utilizing the novel tank test, we examine intra-and inter-session habituation and demonstrate robust habituation responses in adult zebrafish. Analyzing the intra-session habituation to novelty further, we also show that selected anxiogenic drugs (caffeine, pentylenetetrazole), as well as stress-inducing alarm pheromone, attenuated zebrafish habituation. Some acute anxiolytic agents, such as morphine and ethanol, while predictably reducing zebrafish anxiety, had no effects on habituation. Chronic ethanol and fluoxetine treatments improved intra-session habituation in zebrafish. In general, our study parallels literature on rodent habituation responses to novelty, and reconfirms zebrafish as a promising model for cognitive neurobehavioral research.
Stress induced by social defeat is a strong modifier of animal anxiety and depression-like phenot... more Stress induced by social defeat is a strong modifier of animal anxiety and depression-like phenotypes. Self-grooming is a common rodent behavior, and has an ordered cephalo-caudal progression from licking of the paws to head, body, genitals and tail. Acute stress is known to alter grooming activity levels and disrupt its patterning. Following 15-17 days of chronic social defeat stress, grooming behavior was analyzed in adult male C57BL/6J mice exhibiting either dominant or subordinate behavior. Our study showed that subordinate mice experience higher levels of anxiety and display disorganized patterning of their grooming behaviors, which emerges as a behavioral marker of chronic social stress. These findings indicate that chronic social stress modulates grooming behavior in mice, thus illustrating the importance of grooming phenotypes for neurobehavioral stress research. (A.V. Kalueff).
Zebrafish (Danio rerio) are widely used in neuroscience research, where their utility as a model ... more Zebrafish (Danio rerio) are widely used in neuroscience research, where their utility as a model organism is rapidly expanding. Low cost, ease of experimental manipulations, and sufficient behavioral complexity make zebrafish a valuable tool for high-throughput studies in biomedicine. To complement the available repositories for zebrafish genetic information, there is a growing need for the collection of zebrafish neurobehavioral and neurological phenotypes. For this, we are establishing the Zebrafish Neurophenome Database (ZND; www.tulane.edu/*znpindex/search) as a new dynamic online open-access data repository for behavioral and related physiological data. ZND, currently focusing on adult zebrafish, combines zebrafish neurophenotypic data with a simple, easily searchable user interface, which allow scientists to view and compare results obtained by other laboratories using various treatments in different testing paradigms. As a developing community effort, ZND is expected to foster innovative research using zebrafish by federating the growing body of zebrafish neurophenotypic data.
The zebrafish (Danio rerio) is rapidly becoming a popular model species in behavioral neuroscienc... more The zebrafish (Danio rerio) is rapidly becoming a popular model species in behavioral neuroscience research. Zebrafish behavior is robustly affected by environmental and pharmacological manipulations, and can be examined using exploration-based paradigms, paralleled by analysis of endocrine (cortisol) stress responses. Discontinuation of various psychotropic drugs evokes withdrawal in both humans and rodents, characterized by increased anxiety. Sensitivity of zebrafish to drugs of abuse has been recently reported in the literature. Here we examine the effects of ethanol, diazepam, morphine and caffeine withdrawal on zebrafish behavior. Overall, discontinuation of ethanol, diazepam and morphine produced anxiogenic-like behavioral or endocrine responses, demonstrating the utility of zebrafish in translational research of withdrawal syndrome.
Kalueff AV. The effects of chronic social defeat stress on mouse grooming behavior and its patter... more Kalueff AV. The effects of chronic social defeat stress on mouse grooming behavior and its patterning. Behavioural Brain
Analysis of habituation is widely used to characterize animal cognitive phenotypes and their modu... more Analysis of habituation is widely used to characterize animal cognitive phenotypes and their modulation. Although zebrafish (Danio rerio) are increasingly utilized in neurobehavioral research, their habituation responses have not been extensively investigated. Utilizing the novel tank test, we examine intra-and inter-session habituation and demonstrate robust habituation responses in adult zebrafish.
As the rate of biomedical discovery is rising exponentially, electronic databases have become par... more As the rate of biomedical discovery is rising exponentially, electronic databases have become particularly effective in organizing and sharing scienti fi c knowledge. Due to a well-characterized genome, robust behavioral responses and physiological similarity to humans, the zebra fi sh ( Danio rerio ) has emerged as a useful species for neurobehavioral research. The growing utility of this model organism requires the development of specialized databases of zebra fi sh neurophenotypes, such as the Zebra fi sh Neurophenome Project (ZNP) ( http://www.tulane.edu/~znpindex/search ). Representing a new bioinformatics-based tool, the ZNP interactive searchable database consolidates neurobehavioral and related physiological phenotypes obtained in various zebra fi sh models and tests. This chapter outlines the contribution of the ZNP to increased accessibility of current zebra fi sh neurobiological knowledge, and discusses how this database may be used for various research projects.
Zebra fi sh are a popular model organism in neuroscience research, recently emerging as an excell... more Zebra fi sh are a popular model organism in neuroscience research, recently emerging as an excellent species to study complex social phenotypes. For example, zebra fi sh actively form shoals, which can be used to quantify their shoaling behaviors. Zebra fi sh also display strong social preference when placed in a tank with conspeci fi c fi sh, a trait that can easily be quanti fi ed in the two-compartment preference test. The mirror biting test, based on mirror image stimulation, is another well-established method for studying zebra fi sh boldness and sociability. This chapter will describe three simple and ef fi cient paradigms-shoaling, social preference, and mirror biting tests-for quantifying social behaviors in adult zebra fi sh. Re fl ecting different aspects of zebra fi sh social phenotypes, these models can be used individually or within a test battery.
Assessing Habituation Phenotypes in Adult Zebrafish: Intra- and Inter-Trial Habituation in the Novel Tank Test
Neuromethods, 2012
Abstract Although adult zebrafish are increasingly utilized as a model organism in neurobehaviora... more Abstract Although adult zebrafish are increasingly utilized as a model organism in neurobehavioral research, their habituation responses have only recently been evaluated in detail. When exposed to a novel environment, zebrafish demonstrate marked habituation ...
Zebra fi sh is rapidly becoming a popular model species for neurobehavioral and psychopharmacolog... more Zebra fi sh is rapidly becoming a popular model species for neurobehavioral and psychopharmacological research. The startle response represents the instinctive, evolutionarily conserved reaction of an organism to novel unexpected and/or aversive stimuli. While startle testing is a well-established assay to study anxietylike behaviors in different species, screening of the startle response and its habituation in zebra fi sh is also an important direction of translational biomedical research. Complementing rich literature on zebra fi sh startle, this chapter outlines a brief and simple protocol to assess the tapping-induced startle response and its inter-and intra-trial habituation in adult zebra fi sh.
Analysis of habituation is widely used to characterize animal cognitive phenotypes and their modu... more Analysis of habituation is widely used to characterize animal cognitive phenotypes and their modulation. Although zebrafish (Danio rerio) are increasingly utilized in neurobehavioral research, their habituation responses have not been extensively investigated. Utilizing the novel tank test, we examine intra-and inter-session habituation and demonstrate robust habituation responses in adult zebrafish. Analyzing the intra-session habituation to novelty further, we also show that selected anxiogenic drugs (caffeine, pentylenetetrazole), as well as stress-inducing alarm pheromone, attenuated zebrafish habituation. Some acute anxiolytic agents, such as morphine and ethanol, while predictably reducing zebrafish anxiety, had no effects on habituation. Chronic ethanol and fluoxetine treatments improved intra-session habituation in zebrafish. In general, our study parallels literature on rodent habituation responses to novelty, and reconfirms zebrafish as a promising model for cognitive neurobehavioral research.
Stress induced by social defeat is a strong modifier of animal anxiety and depression-like phenot... more Stress induced by social defeat is a strong modifier of animal anxiety and depression-like phenotypes. Self-grooming is a common rodent behavior, and has an ordered cephalo-caudal progression from licking of the paws to head, body, genitals and tail. Acute stress is known to alter grooming activity levels and disrupt its patterning. Following 15-17 days of chronic social defeat stress, grooming behavior was analyzed in adult male C57BL/6J mice exhibiting either dominant or subordinate behavior. Our study showed that subordinate mice experience higher levels of anxiety and display disorganized patterning of their grooming behaviors, which emerges as a behavioral marker of chronic social stress. These findings indicate that chronic social stress modulates grooming behavior in mice, thus illustrating the importance of grooming phenotypes for neurobehavioral stress research. (A.V. Kalueff).
Zebrafish (Danio rerio) are widely used in neuroscience research, where their utility as a model ... more Zebrafish (Danio rerio) are widely used in neuroscience research, where their utility as a model organism is rapidly expanding. Low cost, ease of experimental manipulations, and sufficient behavioral complexity make zebrafish a valuable tool for high-throughput studies in biomedicine. To complement the available repositories for zebrafish genetic information, there is a growing need for the collection of zebrafish neurobehavioral and neurological phenotypes. For this, we are establishing the Zebrafish Neurophenome Database (ZND; www.tulane.edu/*znpindex/search) as a new dynamic online open-access data repository for behavioral and related physiological data. ZND, currently focusing on adult zebrafish, combines zebrafish neurophenotypic data with a simple, easily searchable user interface, which allow scientists to view and compare results obtained by other laboratories using various treatments in different testing paradigms. As a developing community effort, ZND is expected to foster innovative research using zebrafish by federating the growing body of zebrafish neurophenotypic data.
The zebrafish (Danio rerio) is rapidly becoming a popular model species in behavioral neuroscienc... more The zebrafish (Danio rerio) is rapidly becoming a popular model species in behavioral neuroscience research. Zebrafish behavior is robustly affected by environmental and pharmacological manipulations, and can be examined using exploration-based paradigms, paralleled by analysis of endocrine (cortisol) stress responses. Discontinuation of various psychotropic drugs evokes withdrawal in both humans and rodents, characterized by increased anxiety. Sensitivity of zebrafish to drugs of abuse has been recently reported in the literature. Here we examine the effects of ethanol, diazepam, morphine and caffeine withdrawal on zebrafish behavior. Overall, discontinuation of ethanol, diazepam and morphine produced anxiogenic-like behavioral or endocrine responses, demonstrating the utility of zebrafish in translational research of withdrawal syndrome.
Uploads
Papers by Ivan Zapolsky