Papers by Isadora Tassinari
Neurochemistry international, Jul 1, 2024
Neurochemical research, Mar 29, 2024
European journal of neuroscience/EJN. European journal of neuroscience, Feb 4, 2024
The FASEB Journal
Neonatal hypoxic‐ischemic encephalopathy (HI) is one the main causes of neurological damage in ne... more Neonatal hypoxic‐ischemic encephalopathy (HI) is one the main causes of neurological damage in newborns. Pregnancy has emerging as a feasible therapeutic window for preventing central nervous system damage and has been shown to decrease early and long‐term HI consequences. However, experimental literature is conflicting and point that variables, such as a previous adaptation period to the exercise (before mating) has decisive impact on swimming consequences to the injured offspring's brain.Briefly, the adaptation period consisted of exposing female rats to 7 days (with increasing time exposure ‐ from 5 to 20 minutes) to a tank (200 cm diameter) filled with water at 32°C. After mating, from gestational day 0 to 21, pregnant rats were submitted to 20min/day of swimming or remained in the standard cages. After birth, seven‐days‐old rats were subjected to the Levine‐Rice HI model (right common carotid artery occlusion followed by 8% O2/60min). Western blotting was used to assess pro...
IBRO Neuroscience Reports
Developmental Neuroscience
The only current treatment for neonatal hypoxia-ischemia (HI) is therapeutic hypothermia (TH), wh... more The only current treatment for neonatal hypoxia-ischemia (HI) is therapeutic hypothermia (TH), which still shows some limitations. Specific effects of TH in the several processes involved in brain injury progression remain unclear. In this study, the effects of TH treatment on developmental parameters, behavioral outcomes, and peripheral leukocytes were evaluated in neonatal male and female rats. In P7, animals were submitted to right common carotid artery occlusion followed by hypoxia (8% oxygen). TH was performed by reducing the animal scalp temperature to 32°C for 5 h. Behavioral parameters and developmental landmarks were evaluated. Animals were euthanized at P9 or P21, and cerebral hemispheres, spleen, and thymus were weighed. White blood cells (WBC) were counted in blood smears. There was a reduction in the weight of the brain hemisphere ipsilateral to the carotid occlusion in HI and TH groups, as well as a reduction in body weight gain and a delay in the opening of the ipsila...
Frontiers in Cell and Developmental Biology, Jan 26, 2022
Since the first evidence suggesting that maternal nutrition can impact the development of disease... more Since the first evidence suggesting that maternal nutrition can impact the development of diseases in the offspring, much has been elucidated about its effects on the offspring's nervous system. Animal studies demonstrated that maternal obesity can predispose the offspring to greater chances of metabolic and neurodevelopmental diseases. However, the mechanisms underlying these responses are not well established. In recent years, the role of the gut-brain axis in the development of anxiety and depression in people with obesity has emerged. Studies investigating changes in the maternal microbiota during pregnancy and also in the offspring demonstrate that conditions such as maternal obesity can modulate the microbiota, leading to long-term outcomes in the offspring. Considering that maternal obesity has also been linked to the development of psychiatric conditions (anxiety and depression), the gut-brain axis is a promising target to be further explored in these neuropsychiatric contexts. In the present study, we review the relationship between maternal obesity and anxious and depressive features, exploring the gut-brain axis as a potential mechanism underlying this relationship.
Metabolic Brain Disease, Jul 2, 2022
Therapeutic hypothermia (TH) is the standard treatment for neonatal hypoxia-ischemia (HI) with a ... more Therapeutic hypothermia (TH) is the standard treatment for neonatal hypoxia-ischemia (HI) with a time window limited up to 6h post injury. However, in uence of sexual dimorphism in the therapeutic window for TH has not yet been elucidated in animal models of HI. Therefore, the aim of this study was to investigate the most effective time window to start TH in male and female rats submitted to neonatal HI. Wistar rats (P7) were divided into the following groups: NAÏVE and SHAM (control groups), HI (submitted to HI) and TH (submitted to HI and TH; 32ºC for 5h). TH was started at 2h (TH-2h group), 4h (TH-4h group), or 6h (TH-6h group) after HI. At P14, animals were subjected to behavioural tests, volume of lesion and reactive astrogliosis assessments. Male and female rats from the TH-2h group showed reduction in the latency of behavioral tests, and decrease in volume of lesion and intensity of GFAP immuno uorescence. TH-2h females also showed reduction of degenerative cells and morphological changes in astrocytes. Interestingly, females from the TH-6h group showed an increase in volume of lesion and in number of degenerative hippocampal cells, associated with worse behavioral performance. Together, these results indicate that TH neuroprotection is time-and sex-dependent. Moreover, TH started later (6h) can worsen volume of brain lesion in females. These data indicate the need to develop speci c therapeutic protocols for each sex and reinforce the importance of early onset of the hypothermic treatment.
International Journal of Developmental Neuroscience
Nowadays, the only treatment for human babies suffering from hypoxia–ischemia (HI) is therapeutic... more Nowadays, the only treatment for human babies suffering from hypoxia–ischemia (HI) is therapeutic hypothermia (TH). However, a better understanding of the specific effects of TH in males and females is important to improve its clinical application. The present study evaluated the short‐term effects of TH on the brain injury and behavioral outcomes in male and female neonatal rats submitted to neonatal HI. Seven‐day‐old Wistar rats underwent a surgery for unilateral occlusion of the right common carotid artery and were exposed to a hypoxic atmosphere (8% oxygen) for 75 min. Then, the animals in the TH group were submitted to TH (scalp temperature of 32°C) for 5 h. In the behavioral tests, no remarkable differences triggered by HI or TH were observed relative to SHAM animals. Only females of the HI group presented lower latency to complete the righting reflex test. TH reduced the volume of brain injury in males, but not in females. The animals of the HI group showed a reduction in the...
Frontiers in Cell and Developmental Biology, 2022
Since the first evidence suggesting that maternal nutrition can impact the development of disease... more Since the first evidence suggesting that maternal nutrition can impact the development of diseases in the offspring, much has been elucidated about its effects on the offspring’s nervous system. Animal studies demonstrated that maternal obesity can predispose the offspring to greater chances of metabolic and neurodevelopmental diseases. However, the mechanisms underlying these responses are not well established. In recent years, the role of the gut-brain axis in the development of anxiety and depression in people with obesity has emerged. Studies investigating changes in the maternal microbiota during pregnancy and also in the offspring demonstrate that conditions such as maternal obesity can modulate the microbiota, leading to long-term outcomes in the offspring. Considering that maternal obesity has also been linked to the development of psychiatric conditions (anxiety and depression), the gut-brain axis is a promising target to be further explored in these neuropsychiatric contex...
Brain Research, 2020
Previous adaptation triggers distinct molecular pathways and modulates early and long-term neurop... more Previous adaptation triggers distinct molecular pathways and modulates early and long-term neuroprotective effects of pregnancy swimming preventing neonatal hypoxia-ischemia damage in rats, Brain Research (2020),
European Heart Journal, 2012
Therapeutic hypothermia (TH) is the standard treatment for neonatal hypoxia-ischemia (HI) with a ... more Therapeutic hypothermia (TH) is the standard treatment for neonatal hypoxia-ischemia (HI) with a time window limited up to 6h post injury. However, influence of sexual dimorphism in the therapeutic window for TH has not yet been elucidated in animal models of HI. Therefore, the aim of this study was to investigate the most effective time window to start TH in male and female rats submitted to neonatal HI. Wistar rats (P7) were divided into the following groups: NAÏVE and SHAM (control groups), HI (submitted to HI) and TH (submitted to HI and TH; 32ºC for 5h). TH was started at 2h (TH-2h group), 4h (TH-4h group), or 6h (TH-6h group) after HI. At P14, animals were subjected to behavioural tests, volume of lesion and reactive astrogliosis assessments. Male and female rats from the TH-2h group showed reduction in the latency of behavioral tests, and decrease in volume of lesion and intensity of GFAP immunofluorescence. TH-2h females also showed reduction of degenerative cells and morpho...
Clinical & Experimental Immunology
Cellular and Molecular Neurobiology
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Papers by Isadora Tassinari