Papers by Iguatemy L O Brunetti
Phytochemistry, 2008
A myeloperoxidase inhibitory kaempferol derivative, namely pterogynoside (1), was isolated from f... more A myeloperoxidase inhibitory kaempferol derivative, namely pterogynoside (1), was isolated from fruits of Pterogyne nitens, along with six known flavonols, kaempferol, afzelin, kaempferitrin, quercetin, isoquercetrin and rutin. The structures of all compounds were elucidated primarily from 1D and 2D NMR spectroscopic analyses, as well as by high resolution mass spectrometry. All flavonols were screened to identify secondary metabolites as potential myeloperoxidase (MPO) inhibitors, and at concentrations of 0.50-50 nM, quercetin (5), isoquercitrin (6) and rutin (7) exhibited strong inhibitory effects with IC 50 values of 1.22 ± 0.01, 3.75 ± 0.02 and 3.60 ± 0.02, respectively. The MPO activity detected for the new derivative 1 was markedly decreased (IC 50 10.3 ± 0.03) when compared with known flavonols 5-7, and interestingly increased when tested against ABTS scavenging activity.
Photochemistry and Photobiology, 1983
Electronically excited triplet species generated during the peroxidase catalyzed aerobic oxidatio... more Electronically excited triplet species generated during the peroxidase catalyzed aerobic oxidation of appropriate substrates efficiently elicit fluorescence from acceptors in micelles, as shown with 9,lO-dibromoanthracene and chlorophyll solubilized by various surfactants. In the case of 9,lO-dibromoanthracene excited by triplet acetone, phosphorescence also can be detected near O2 depletion. The significant implications of this study are that micelle-solubilized chlorophyll is an excellent detector of enzyme-generated triplet carbonyl species, as confirmed with several systems, and that the use of micelles make it possible to extend 'photobiochemistry without light' to other photobiologically important, yet water insoluble acceptors.
Fitoterapia, 2006
Maytenus ilicifolia is an important plant with potential on cancer treatment and has been largely... more Maytenus ilicifolia is an important plant with potential on cancer treatment and has been largely used in Brazil and other countries. We have evaluated the crude ethanolic extract of M. ilicifolia as a potential antioxidant source using an assay based on the bleaching of the radical monocation 2,2′-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS •+) and by HOCl scavenger capacity. Trolox and uric acid were used as positive controls. The results indicated M. ilicifolia root bark as a great source of antioxidants based on its potential as scavenger of radicals.
Revista Brasileira de Ciências Farmacêuticas, 2007
Biopharmaceutics & Drug Disposition, 2012
The combination of isoniazid (INH), rifampicin (RMP) and pyrazinamide (PYR) is used in the treatm... more The combination of isoniazid (INH), rifampicin (RMP) and pyrazinamide (PYR) is used in the treatment of tuberculosis. Although this treatment is effective in most clinical cases, the side-effects and the development of mycobacterium resistance have hindered its success. There is evidence that the combination of INH, RMP and ciprofloxacin (CIPRO) is useful in the treatment of tuberculosis. However, the influence of this drug combination on the hepatotoxicity of INH is unknown. In this study, the safety of combined INH, RMP and CIPRO was evaluated. Male albino rabbits (n = 20) were divided into four groups and subjected to multiple oral doses for 7 days according to the following treatments: water (group 1); 50 mg/kg INH (group 2); 50 mg/kg INH + 100 mg/kg RMP (group 3) and 50 mg/kg INH + 100 mg/kg RMP + 50 mg/kg CIPRO (group 4). Blood samples were taken before and after treatments for the determination of ALT, AST, ALP and bilirubin to assess hepatotoxicity. For pharmacokinetic analysis, serial blood samples were collected over 24 h on day 7 of treatment. Plasma concentrations of INH and acetylisoniazid (AcINH) were determined by HPLC. Biochemical parameters did not show any statistically significant differences between the groups that received the drug combinations. The pharmacokinetic profile of INH was also similar for both groups of combinations. These findings allow us to infer that the inclusion of CIPRO did not increase the risk of hepatotoxicity when compared with the classic combination of INH and RMP.
BMC Nephrology, 2015
Background: In view of the prevalence of oxidative stress in chronic kidney disease (CKD) patient... more Background: In view of the prevalence of oxidative stress in chronic kidney disease (CKD) patients, the loss of lowmolecular-weight biomolecules by hemodialysis and the antioxidant potential of some uremic solutes that accumulate in CKD, we used in vitro model systems to test the antioxidant potential of the following uremic solutes: uric acid, hippuric acid, p-cresol, phenol, methylguanidine, L-arginine, L-tyrosine, creatinine and urea. Methods: The in vitro antioxidant efficiencies of the uremic solutes, isolated or in mixtures, were tested with the following assays: i) ABTS radical cation decolorization assay; ii) hypochlorous acid (HOCl/OCl −) scavenging activity; iii) superoxide anion radical (O 2 •-) scavenging activity; iv) crocin bleaching assay (capture of peroxyl radical, ROO •); v) hydrogen peroxide (H 2 O 2) scavenging activity. Results: Four of the tested uremic solutes (p-cresol, phenol, L-tyrosine, uric acid) were effective antioxidants and their IC 50 were found in three model systems: ABTS •+ , HOCl/OCl − and crocin bleaching assay. In the 4-solutes mixtures, each one of the solute captured 12.5% for the IC 50 of the mixture to ABTS •+ or HOCl/OCl − , exhibiting a virtually exact additive effect. In the 2-solutes mixtures, for ROO • capture, it was observed the need of more mass of uremic solutes to reach an IC 50 value that was higher than the projected IC 50 , obtained from the IC 50 of single solutes (25% of each, in the binary mixtures) in the same assay. In model systems for O 2 •and H 2 O 2 , none of the uremic solutes showed scavenging activity. Conclusions: The use of the IC 50 as an analytical tool to prepare and analyze mixtures allows the determination of their scavenging capacities and may be useful for the assessment of the antioxidant status of biological samples under conditions of altered levels of the endogenous antioxidant network and/or in the employment and monitoring of exogenous antioxidant therapy.
Latin American Journal of Pharmacy, 2008
European Journal of Dentistry, 2011
Objectives:The aim of this study was to assess a suggested association between periodontitis and ... more Objectives:The aim of this study was to assess a suggested association between periodontitis and renal insufficiency by assaying kidney disease markers.Methods:Variables used to diagnose periodontitis were: (i) probing pocket depth (PPD), (ii) attachment loss (AL), (iii) bleeding on probing (BOP), (iv) plaque index (PI) and (v) extent and severity index. Blood and urine were collected from 60 apparently healthy non-smokers (men and women), consisting of a test group of 30 subjects with periodontitis (age 46±6 yrs) and a control group of 30 healthy subjects (age 43±5 yrs). Kidney function markers (urea, creatinine, uric acid and albumin contents) were measured in the serum and urine. Also, the glomerular filtration rate was estimated from creatinine clearance, from the abbreviated Modification of Diet in Renal Disease formula and from the albumin : creatinine ratio in a 24–h sample of urine.Results:It was found that the control group had a greater mean number of teeth than the test group and that the two groups also differed in PPD, AL, BOP and PI, all these variables being higher in the test group (P=0.006). For the extent and severity index of both PPD and AL, the test group had much higher medians of both extent and severity than the control group (P=0.001). With regard to kidney function, none of the markers revealed a significant difference between the control and test groups and all measured values fell within the reference intervals.Conclusions:It is proposed that severe periodontitis is not associated with any alteration in kidney function.
Rev Bras Farmacogn, 2008
Considerando-se a qualidade dos fi toterápicos, é importante salientar que a preocupação com esta... more Considerando-se a qualidade dos fi toterápicos, é importante salientar que a preocupação com esta questão inclui rigoroso acompanhamento das diferentes etapas do desenvolvimento e produção destes produtos, desde a coleta do vegetal até a disponibilidade do produto fi nal. Neste trabalho, foram realizados o controle da qualidade, o potencial antioxidante como também ensaios biológicos in vitro do fruto da goiabeira (Psidium guajava L.), para o desenvolvimento de uma formulação fi tocosmética. Os resultados obtidos mostraram que o fruto apresenta taninos e fl avonóides, bem como atividades antioxidante e antimicrobiana. A análise microbiológica não apresentou crescimento de patógenos na formulação desenvolvida entre os outros testes realizados. Destaca-se a importância do estabelecimento do controle da qualidade para as plantas, a fi m de que sejam utilizadas para o desenvolvimento de uma formulação fi tocosmética segura, efi caz e com qualidade.
Pharmaceutical Biology, 2006
... glucose and polyuria (Anon, 1968; Kohli & Singh, 1985; Achrekar et al., 1991; Grover et a... more ... glucose and polyuria (Anon, 1968; Kohli & Singh, 1985; Achrekar et al., 1991; Grover et al., 20012002; Vikrant et al ... Kar A, Choudhary BK, Bandyopadhyay NG (2003): Comparative evaluation of hypoglycaemic activity of some Indian medicinal plants in alloxan diabetic rats. ...
Revista Da Associacao Medica Brasileira, Dec 1, 2008
Jornal De Pediatria, 2008
OBJETIVO: Determinar, entre um grupo de crianças e adolescentes eutróficos, os valores de glicemi... more OBJETIVO: Determinar, entre um grupo de crianças e adolescentes eutróficos, os valores de glicemia e insulinemia de jejum e de índice homeostasis model assessment (HOMA). MÉTODOS: Estudo de corte transversal realizado em duas escolas públicas de Ribeirão ...
Rev Bras Anal Clin, 1994
... 1 / 1, ADOLEC, seleciona. para imprimir. Fotocópia. Rego, Eduardo Magalhäes. Cilento, Giusepp... more ... 1 / 1, ADOLEC, seleciona. para imprimir. Fotocópia. Rego, Eduardo Magalhäes. Cilento, Giuseppe. Id: 136403. Autor: Fonseca, Luiz Marcos da; Brunetti, Iguatemy Lourenço; Rego, Eduardo Magalhaes; Garcia, Aglair Bergamo; Cilento, Giuseppe; Falcao, Roberto Passeto. ...
BMC Nephrology, 2015
In view of the prevalence of oxidative stress in chronic kidney disease (CKD) patients, the loss ... more In view of the prevalence of oxidative stress in chronic kidney disease (CKD) patients, the loss of low-molecular-weight biomolecules by hemodialysis and the antioxidant potential of some uremic solutes that accumulate in CKD, we used in vitro model systems to test the antioxidant potential of the following uremic solutes: uric acid, hippuric acid, p-cresol, phenol, methylguanidine, L-arginine, L-tyrosine, creatinine and urea. The in vitro antioxidant efficiencies of the uremic solutes, isolated or in mixtures, were tested with the following assays: i) ABTS radical cation decolorization assay; ii) hypochlorous acid (HOCl/OCl(-)) scavenging activity; iii) superoxide anion radical (O2(•-)) scavenging activity; iv) crocin bleaching assay (capture of peroxyl radical, ROO(•)); v) hydrogen peroxide (H2O2) scavenging activity. Four of the tested uremic solutes (p-cresol, phenol, L-tyrosine, uric acid) were effective antioxidants and their IC50 were found in three model systems: ABTS(•+), HOCl/OCl(-) and crocin bleaching assay. In the 4-solutes mixtures, each one of the solute captured 12.5% for the IC50 of the mixture to ABTS(•+) or HOCl/OCl(-), exhibiting a virtually exact additive effect. In the 2-solutes mixtures, for ROO(•) capture, it was observed the need of more mass of uremic solutes to reach an IC50 value that was higher than the projected IC50, obtained from the IC50 of single solutes (25% of each, in the binary mixtures) in the same assay. In model systems for O2(•-) and H2O2, none of the uremic solutes showed scavenging activity. The use of the IC50 as an analytical tool to prepare and analyze mixtures allows the determination of their scavenging capacities and may be useful for the assessment of the antioxidant status of biological samples under conditions of altered levels of the endogenous antioxidant network and/or in the employment and monitoring of exogenous antioxidant therapy.
Uploads
Papers by Iguatemy L O Brunetti