Ngan Huang

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C. Michael Hall

University of Canterbury/Te Whare Wānanga o Waitaha

James McDonald

Bangor University

Ashraf M. Salama

Northumbria University

David Seamon

Kansas State University

Jon R Sayers

The University of Sheffield

Marta Florio

Harvard Medical School

Paixão-Côrtes Vanessa

Universidade Federal do Rio Grande do Sul

Cristian Araneda

Universidad de Chile

Thanh L To

Vietnam National University, Hanoi

Gareth Enticott

Cardiff University

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Design of the SSRF Magnet Lattice

Commemorating the Past and Looking Towards the Future, 2002

The Shanghai Synchrotron Radiation Facility (SSRF) [1], a 3.5GeV third generation light source, w... more The Shanghai Synchrotron Radiation Facility (SSRF) [1], a 3.5GeV third generation light source, will be built at Pudong of Shanghai, China. It is optimised to provide high brightness and high flux X-ray photon beams in energy from 0.1 to 40keV. The magnet lattice is composed of 20 DBA cells resulting in a ring that is about 10nm·rad in emittance and 396 meter in circumference. It provides 10 straight sections of 7.24 meter and 10 straight sections of 5.0 meter for the inclusion of insertion devices. This paper reports on the design of DBA cells and simulation of the dynamic aperture.

Abstract 83: Nanopatterned Collagen Scaffolds Promote Blood Perfusion in the Ischemic Limb

Arteriosclerosis, Thrombosis, and Vascular Biology, May 1, 2014

Cell-based approaches to improve blood perfusion are promising for treatment of peripheral arteri... more Cell-based approaches to improve blood perfusion are promising for treatment of peripheral arterial disease (PAD). However, cell delivery alone is limited by poor cell survival and the lack of organization among newly formed vessels. We hypothesized that parallel-aligned nanofibrillar scaffolds may guide endothelial cell (EC) assembly and accelerate the formation of organized vascular networks that enhance blood perfusion, in comparison to cell delivery in saline. We fabricated nanofibrillar collagen scaffolds with parallel-oriented configuration or randomly oriented configuration. Primary human ECs aligned along the direction of the nanofibrils of the aligned nanofibrillar scaffold, as shown by scanning electron microscopy, where the arrow denotes the orientation of nanofibrils (Fig 1). The ECs also had organized cytoskeleton within 20 degrees from the direction of the aligned nanofibrils. To test the efficacy of the aligned nanofibrillar scaffolds in improving neovascularization in vivo, we induced unil...

Abstract 269: Collagen Topographical Patterning Modulates Endothelial Cell Morphology, Gene Expression and Function

Arteriosclerosis, Thrombosis, and Vascular Biology, 2012

Endothelial cells (ECs) exposed to laminar shear stress align in the direction of blood flow and ... more Endothelial cells (ECs) exposed to laminar shear stress align in the direction of blood flow and express low levels of adhesion molecules. However, it is unknown whether these biological responses result from shear stress per se, or if they can be recapitulated using topography cues from the underlying extracellular matrix (ECM). We hypothesized that collagen topographical patterning can regulate EC assembly and function in the absence of shear stress. We fabricated collagen-coated polydimethylsiloxane microchannels (30μm wide) to induce EC alignment. When grown on microchannels, the ECs underwent striking re-organization of their actin cytoskeleton (aligned within 10 degrees of the microchannel direction) and their focal adhesions (Fig A). Mimicking the reported EC responses to laminar shear stress, aligned ECs were more quiescent (reduced Ki67 expression), and less adhesive for monocytes (with 50% reductions in the expression of intercellular adhesion molecule 1, and in monocyte a...

Journal of Visualized Experiments www.jove.com

In the United States, peripheral arterial disease (PAD) affects about 10 million individuals, and... more In the United States, peripheral arterial disease (PAD) affects about 10 million individuals, and is also prevalent worldwide. Medical therapies for symptomatic relief are limited. Surgical or endovascular interventions are useful for some individuals, but long-term results are often disappointing. As a result, there is a need for developing new therapies to treat PAD. The murine hindlimb ischemia preparation is a model of PAD, and is useful for testing new therapies. When compared to other models of tissue ischemia such as coronary or cerebral artery ligation, femoral artery ligation provides for a simpler model of ischemic tissue. Other advantages of this model are the ease of access to the femoral artery and low mortality rate. In this video, we demonstrate the methodology for the murine model of unilateral hindimb ischemia. The specific materials and procedures for creating and evaluating the model will be described, including the assessment of limb perfusion by laser Doppler im...

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