This study was aimed at evaluating the nutritional value of the mesocarp of Raphia hookeri (Rh) f... more This study was aimed at evaluating the nutritional value of the mesocarp of Raphia hookeri (Rh) fruit and the effect of powder and aqueous extract of the fruit on hematological parameters in rats which have undergone neurotoxicity by aluminum chloride. The nutritional content was evaluated with the standard method. Seven groups of six Wistar rats were used, neurotoxicity was induced by 4.2 mg/kg of body weight of aluminum chloride 3 times a week intraperitonealy with treatment. Rat treatment was as follow: aqueous extract at 200 and 400 mg/kg body weight, 5 and 10% of formulation, negative, positive and normal groups. The experiment lasted 28 days. The data obtained from the nutritive value showed that Rh mesocarp is a good source of lipids (48.97%), fibers (25.82%), calcium (3183.3mg/100g of dry matter), potassium (1218.3 mg/100g of DM), zinc (0.88 mg/100g of DM) and selenium (8.6 mg/100g of DM). Nevertheless, it contains a little amount of phytic acid and hydrocyanic acid which is acceptable for human consumption. It can then be used in many formulations as a source of these nutrients. Aluminum administration indicates the reduction in food intake, a low weight gain and Hematological alteration in the Positive control group. However, consumption of Rh mesocarp indicated an increase in food intake, weight gain and a restoration of hematological parameters to the normal level with the best values in groups that were administered Rh powders (Rh5% and Rh10%). Raphia hookeri mesocarp is a rich source of nutrients involved in the prevention of hematological disorder.
The effect of sunlight on the quality of groundnut oil in comparison with the same oil samples st... more The effect of sunlight on the quality of groundnut oil in comparison with the same oil samples stored in the dark at room temperature was aimed to study. The oil samples were exposed to sunlight (34-40°C) and ambient storage (~24°C in the dark) for 90 days. The samples were collected every 30 days for the analysis of their physicochemical properties including peroxide value (PV), p-anisidine (p-AnV), total oxidation (TOTOX), thiobarbituric acid (TBA), free fatty acid (FFA), color, induction period on Rancimat and fatty acid composition. At the beginning of the experiment, the PV, p-AnV, TOTOX, TBA and FFA values of groundnut oil were 10.31 meq O2/kg, 4.39, 25.01, 0.99 and 1.49% respectively; color 1.40R, 22.00Y and induction time (IT) 5.33 h. After 90 days storage at room temperature, the PV, pAn -V, TOTOX, TBA and FFA values were respectively ranged between 10.31-21.32 meq O2/kg, 4.39-7.36, 25.01-50.12, 0.99-2.42 ppm and 1.49-2.64%; the color in the red and yellow unit 1.40-1.20R and 22.00-19.00Y respectively; and the induction time 5.33-3.77 h. However, the same parameters at the end of exposure to sunlight were ranged in the same order, between 10.31-43.47 meq O2/kg, 4.39-22.24, 25.01-109.18, 0.99-3.25 ppm and 1.49-5.83% respectively; the color in the red and yellow units 1.40-0.50R and 22.00-1.30Y respectively; and the induction time 5.33-0.03 h. Results also showed that the amount of linoleic and gadoleic acids significantly dropped under sunlight compared to dark at room temperature. Sunlight significantly reduced the quality of groundnut oil. This oil should be stored in the dark at room temperature.
Cameroon Journal of Experimental Biology, Mar 10, 2021
The current study was conducted in four localities in the West region of Cameroon from May to Sep... more The current study was conducted in four localities in the West region of Cameroon from May to September 2019, to know the pre-treatments made on given to beef hides before their use in different dishes, as well as the ingredients used in the preparation of pepper soup beef hides through household surveys. A total of 307 housewives were surveyed interviewed. The data was collected using a questionnaire and further analyzed using Sphinx Plus2 V5 software. Results showed that 54.6 to 71% participants consumed beef hides monthly. More than 50% participants purchased their beef hides at the butchery at a price varying between XAF 1,000 for 1Kg of uncleaned smoked beef hides and XAF 3,000 for 2Kg of clean smoked beef hides. The results also showed that there were five main stages in the preparation of beef hides and housewives had a greater preference of beef hides from the head and buttocks. The pre-cooking of beef hides was done between 180 and 240 minutes, with a quantity of water varying between 4 and 5 liters for 1 to 2Kg of beef hides, on the wood fire. “Eru” and “Yellow achu sauce” were cited as the main sauces in which beef hides were used at 95.40% and 85% respectively. Besides, more than 70% of housewives used a maximum of five ingredients for the preparation of pepper soup beef hides. Our findings revealed, interesting insights on the steps and ingredients used when preparing pepper soup beef hides that will be of great importance in the development of better formulation for pepper soup beef hides preparation
The effects of different processing methods on proximate composition, total phenolic content, ant... more The effects of different processing methods on proximate composition, total phenolic content, antioxidant activity, lipid oxidation, and mineral contents of sunflower seeds produced in Far North Region of Cameroon were evaluated. Mean moisture, ash, lipid, protein, fiber, and carbohydrate contents of raw sunflower seeds were 6.60%, 2.55%, 44.65%, 20.17%, 4.08%, and 21.25%, respectively. The changes in moisture, ash (excepted in boiled samples), lipid, protein, fiber, and carbohydrate (excepted in roasted samples) were found to be significant for all cooking methods. Ash and lipid contents of samples roasted at 120°C were found to be significant when compared with other cooking methods. Antioxidant activity increased with treatment. After processing, the acid, peroxide, and thiobarbituric acid values increased significantly, whereas iodine value decreased. The roasting process improved the induction time, and samples roasted at 120°C were found to have the highest induction time (2.29 ± 0.09 hr). Raw sunflower seeds were good sources of potassium (K), magnesium (Mg), calcium (Ca), iron (Fe), zinc (Zn), and manganese (Mn). Increase in contents of Ca, Mg, Zn, Cu, and Fe was observed during processing. Roasting compared with boiling appeared to be the best cooking method of sunflower seeds concerning nutrient content, antioxidant stability, and lipid stability.
Population Medicine considers the following types of articles: • Research Papers-reports of data ... more Population Medicine considers the following types of articles: • Research Papers-reports of data from original research or secondary dataset analyses. • Review Papers-comprehensive, authoritative, reviews within the journal's scope. These include both systematic reviews and narrative reviews. • Short Reports-brief reports of data from original research. • Policy Case Studies-brief articles on policy development at a regional or national level. • Study Protocols-articles describing a research protocol of a study. • Methodology Papers-papers that present different methodological approaches that can be used to investigate problems in a relevant scientific field and to encourage innovation. • Methodology Papers-papers that present different methodological approaches that can be used to investigate problems in a relevant scientific field and to encourage innovation. • Letters to the Editor-a response to authors of an original publication, or a very small article that may be relevant to readers. • Editorials-articles written by the Editorial Board or by invited experts on a specific topic. Research Papers Articles reporting research may be full length or brief reports. These should report original research findings within the journal's scope. Papers should generally be a maximum of 4000 words in length, excluding tables, references, and abstract and key points of the article, whilst it is recommended that the number of references should not exceed 36.
Population Medicine considers the following types of articles: • Research Papers-reports of data ... more Population Medicine considers the following types of articles: • Research Papers-reports of data from original research or secondary dataset analyses. • Review Papers-comprehensive, authoritative, reviews within the journal's scope. These include both systematic reviews and narrative reviews. • Short Reports-brief reports of data from original research. • Policy Case Studies-brief articles on policy development at a regional or national level. • Study Protocols-articles describing a research protocol of a study. • Methodology Papers-papers that present different methodological approaches that can be used to investigate problems in a relevant scientific field and to encourage innovation. • Methodology Papers-papers that present different methodological approaches that can be used to investigate problems in a relevant scientific field and to encourage innovation. • Letters to the Editor-a response to authors of an original publication, or a very small article that may be relevant to readers. • Editorials-articles written by the Editorial Board or by invited experts on a specific topic. Research Papers Articles reporting research may be full length or brief reports. These should report original research findings within the journal's scope. Papers should generally be a maximum of 4000 words in length, excluding tables, references, and abstract and key points of the article, whilst it is recommended that the number of references should not exceed 36.
Annals of the University "Dunărea de Jos" of Galaţi, Mar 29, 2022
This work aimed to assess the oxidative stability of cottonseed oil enriched with natural antioxi... more This work aimed to assess the oxidative stability of cottonseed oil enriched with natural antioxidants from Cameroonian plant leaves extracts. Total polyphenols, flavonoids, and antioxidant activity via DPPH radical scavenging assay were assessed in ethanolic extracts. These extracts were added to cottonseed oil at concentrations of 200, 500, 1250, and 2000 ppm. The cottonseeds' oil oxidative stability was evaluated by determining their induction/stability times on Rancimat (120±1.6°C) and by measuring their oxidative state in Schaal oven during 30 days (samples were removed after 10 days) of storage at 60°C. The outcomes showed that Canarium schweinfurthii, Trichoscypha acuminate, Dacryodes edulis, and Psidium guajava leaves extracts are rich in polyphenols (18.56 to 42.70 mg/g) and flavonoids (2.14 to 5.36 mg/g). The ethanolic extracts, above 1250 ppm, were also efficient like synthetic antioxidants in inhibiting the oxidation of cottonseeds oil at high temperatures. The evaluation of oxidation parameters of enriched cottonseeds oil showed that different extracts at 2000 ppm can limit the appearance of both primary and secondary oxidation products in oils.
Soya bean (variety TGX 1835-10E) is a legume commonly produced and consumed in Cameroon. Despite ... more Soya bean (variety TGX 1835-10E) is a legume commonly produced and consumed in Cameroon. Despite its affordability compared to animal sources of proteins, protein energy malnutrition (PEM) is still observed in the country and especially in rural areas. This can be attributed the way the available soya bean is processed which can easily affect its physicochemical properties and reduce its nutritional value and functional properties. This study was conducted in order to evaluate the effect of different processing methods on the nutritional composition, phytochemical and functional properties of soya bean. The beans were divided into nine groups that were processed differently and analyzed for their total phenolic content, antioxidant activity, oil quality and nutritional composition and the functional properties of their flours. Results showed that the total phenolic content was found to be ranged between 99.84 - 216.85 mg GAE/g and significantly increased with roasting and decreased ...
e present study aimed at determining the optimal conditions for extraction of total phenolic comp... more e present study aimed at determining the optimal conditions for extraction of total phenolic compounds from soybean (Glycine max) meal, a by-product of the soybean seeds industry, using a green protocol with microwave-assisted extraction (MAE). A facecentered composite design (FCCD) was used for optimization. Based on a screening aimed to determine the factors that significantly influenced the responses, a 50% hydro-ethanolic solution was used with solvent/dry matter ratio (60/1-110/1), power (120-270 W), and time (0-10 min) as factors, while the responses studied were total phenolic and flavonoid contents. FTIR, TLC, DPPH, and FRAP anti-oxidants tests were used to characterize the extracts obtained with optimum conditions. e factors that significantly influenced both responses were the individual effect of all factors, the interaction between solvent/dry matter ratio and extraction time, the quadratic effect of solvent/dry matter ratio, and power for total phenolic content, while only the quadratic effect of power significantly influenced the flavonoid content. e highest contents of phenols (13.09 mg GAE/g) and flavonoid (7.39 mg CE/g) were obtained at 120 W for 0.16 min with a solvent/dry matter ratio of 60/1. ATR-FTIR spectra indicated the presence of polyphenolic compounds in the extract, namely flavonoids. TLC indicated the presence of at least nine compounds in the extract, among which catechin and quercetin were identified with respective Rf of 0.98 and 0.93. DPPH assay showed the antioxidant capacity for the extract with an IC 50 of 194.98 µg/ml. RSM permitted us to develop a green protocol for maximum extraction of polyphenols from soybean seeds waste using less solvent, low power, and a reduced time in MAE.
International Journal of Biological and Chemical Sciences, Aug 17, 2020
Fish with higher nutrient qualities can be used to solve malnutrition problems in Africa. So the ... more Fish with higher nutrient qualities can be used to solve malnutrition problems in Africa. So the aim of this study was to evaluate the effect of local smoking on proximate composition and lipid oxidation of Chrysichthys nigrodigitatus collected in Cameroon. The proximate composition was assessed using standard methods while lipid quality assessment was based on the determination of biochemical indexes. A change on fatty acid composition was evaluated by gas chromatography while minerals composition was evaluated by spectrophotometer. On nutritional point of view, raw fish have high protein, lipid and ash contents (51.87, 37.48 and 8.55 g/100 g dry weight respectively). Unsaturated fatty acid may be up to 57% of the total fatty acids. Raw C. nigrodigitatus contain reasonable amount of minerals. Smoking process increased ash and lipid content while proteins and moisture contents are reduced in these fish. The iodine value of C. nigrodigitatus was reduced while acid, peroxide and TOTOX values increased during smoking. The marked decrease of polyunsaturated fatty acids was noted during processing. Mineral contents were affected by smoking process. Calcium, magnesium, potassium, sodium, manganese and iron contents increased significantly. In conclusion, hot smoking enhances nutrients content of C. nigrodigitatus while significant reduction in lipid quality was recorded.
European Food Research and Technology, Dec 11, 2017
The objective of this study was to evaluate the effect of ginger roots methanolic extract on the ... more The objective of this study was to evaluate the effect of ginger roots methanolic extract on the stability of palm olein during accelerated storage. After analysis of the extract by the measurement of its total phenolic content by colorimetry and detection of some of its phenolic antioxidants by HPLC-DAD (high performance liquid chromatography-diode array detector) and ESI-MS (Electrospray ionization-mass spectrometer), preliminary antioxidants tests have been conducted. After these tests, the extract has been added in refined palm olein at concentration 200-1800 ppm for investigating its effect on it oxidative stability. Butylated hydroxytoluene (BHT) at it legal concentration of 200 ppm served as positive control, while palm olein without additive served as negative control. Induction time, chemical indexes (peroxide, p-Anisidine, total oxidation, thiobarbituric acid and iodine values) and changes in linoleic acid profile (analyzed by gas chromatography coupled to a flame ionization detector) of oil samples were evaluated. Results of these investigations showed ginger roots methanolic extract to be rich in phenolic antioxidants. Ferulic acid and 6-gingerol were the antioxidants detected. The extract was also efficient in reducing palm olein oxidation on Rancimat and Schaal oven test of 30 days at 70 °C. Ginger roots extract might be a viable source of natural antioxidants for stabilization of palm olein.
The energy density of the complementary gruels can be increased by the use of sprouted flours. Th... more The energy density of the complementary gruels can be increased by the use of sprouted flours. This led us to determine the conditions for obtaining and using sprouted corn flour for optimal fluidification of fermented cassava flour gruels. To do this, the germinated corn flour (GCF) varieties Atp and Kassaï was produced according to the response surface methodology and the Doehlert plan was used with factors such as soaking (12-48 h) and germination (24-96 h) times. Once obtained, these GCF were used to increase the energy density of cassava flour gruels with flow velocity as response. The Doehlert plan was also used with fermented cassava flour concentration (17.5-30 %) and optimised sprouted corn flour mass (1-5 g) as factors. The experimental design that had been performed indicated that the effectiveness of corn amylase flour to fluidify the gruels depends not only to the conditions of production (soaking and germination) (p < 0.05) but also their incorporation during the preparation (corn amylase-fermented cassava flour) (p < 0.05). The rich corn amylase flour can be obtained by soaking Kassaï and Atp varieties for 20 h and 28 h respectively and germinated for 90.82 h. Application of these GCF during the preparation of fermented cassava flour gruels has shown that to obtain gruels with flow velocities between 100-160 mm/30s, it was necessary to couple 1.16 g of sprouted corn flour variety Kassaï for 26.23 % of fermented cassava flour and 1.12 g of corn flour variety Atp for 25.94 % of fermented cassava flour. The use of these couples has made it possible to multiply the energy density of the gruels by 6.55 and 6.49, respectively. In view of these results, it is therefore advisable to use the germinated corn flours produced under the conditions obtained to fluidise and increase the energy density of the fermented cassava gruels.
The effects of refrigeration at 4 °C during 9 days on the quality and stability of catfish oil we... more The effects of refrigeration at 4 °C during 9 days on the quality and stability of catfish oil were evaluated using a change in fatty acid composition by gas chromatography (GC), commonly used analytical indexes (acid and peroxide values), and analysis by Fourier transform infrared (FTIR) spectroscopy. The results revealed that lipid deterioration, hydrolysis and oxidation occurred throughout the cold storage (4 °C). Refrigeration induced the lipolysis of triglycerides by lipases and phospholipases. It also affected the fatty acids composition of the catfish. The progressive loss of unsaturation was monitored by the decrease in the absorbance band at 3012 cm-1 on FTIR spectra and the lowest value was observed in the catfish muscle at 9 days of refrigeration. Eicosapentaenoic C20:5ω3 (EPA) and docosahexaenoic C22:6ω3 (DHA) acids were the polyunsaturated fatty acids most affected during refrigeration. Refrigeration for less than 5 days was found to be the best conditions for the preservation of the catfish.
This study investigates the effect of soursop flowers methanolic extract on the physicochemical c... more This study investigates the effect of soursop flowers methanolic extract on the physicochemical characteristics of palm olein during accelerated storage. After analysis of the extract by determining its total phenolic content by colorimetry and identification of some of its phenolic antioxidants by HPLC-DAD(Diode Array Detector), preliminary antioxidants tests have been done. After that, the extract was added in palm olein at concentrations 200-1800 ppm. BHT, at 200 ppm served as standard besides the Control. Induction time, peroxide, p-anisidine, Total oxidation (TOTOX), thiobarbituric acid and iodine values, as well as changes in linoleic acid profile Gas Chromatography/Flamme Ionization Detector (GC/FID) of oil during the storage were evaluated. Results showed soursop flower extract to be rich in phenolic antioxidants and to be efficient, at all concentrations, in delaying palm olein oxidation on Rancimat and accelerated Schaal oven test of 30 days at 70°C. Soursop flowers might be used as potent source of antioxidants for the stabilization of palm olein.
Antioxidant and antidiabetic activities of a polyphenol rich extract obtained from Abelmoschus es... more Antioxidant and antidiabetic activities of a polyphenol rich extract obtained from Abelmoschus esculentus (okra) seeds using optimized conditions in microwave-assisted extraction (MAE). Front. Nutr. :. doi: .
This study was aimed at evaluating the nutritional value of the mesocarp of Raphia hookeri (Rh) f... more This study was aimed at evaluating the nutritional value of the mesocarp of Raphia hookeri (Rh) fruit and the effect of powder and aqueous extract of the fruit on hematological parameters in rats which have undergone neurotoxicity by aluminum chloride. The nutritional content was evaluated with the standard method. Seven groups of six Wistar rats were used, neurotoxicity was induced by 4.2 mg/kg of body weight of aluminum chloride 3 times a week intraperitonealy with treatment. Rat treatment was as follow: aqueous extract at 200 and 400 mg/kg body weight, 5 and 10% of formulation, negative, positive and normal groups. The experiment lasted 28 days. The data obtained from the nutritive value showed that Rh mesocarp is a good source of lipids (48.97%), fibers (25.82%), calcium (3183.3mg/100g of dry matter), potassium (1218.3 mg/100g of DM), zinc (0.88 mg/100g of DM) and selenium (8.6 mg/100g of DM). Nevertheless, it contains a little amount of phytic acid and hydrocyanic acid which is acceptable for human consumption. It can then be used in many formulations as a source of these nutrients. Aluminum administration indicates the reduction in food intake, a low weight gain and Hematological alteration in the Positive control group. However, consumption of Rh mesocarp indicated an increase in food intake, weight gain and a restoration of hematological parameters to the normal level with the best values in groups that were administered Rh powders (Rh5% and Rh10%). Raphia hookeri mesocarp is a rich source of nutrients involved in the prevention of hematological disorder.
The effect of sunlight on the quality of groundnut oil in comparison with the same oil samples st... more The effect of sunlight on the quality of groundnut oil in comparison with the same oil samples stored in the dark at room temperature was aimed to study. The oil samples were exposed to sunlight (34-40°C) and ambient storage (~24°C in the dark) for 90 days. The samples were collected every 30 days for the analysis of their physicochemical properties including peroxide value (PV), p-anisidine (p-AnV), total oxidation (TOTOX), thiobarbituric acid (TBA), free fatty acid (FFA), color, induction period on Rancimat and fatty acid composition. At the beginning of the experiment, the PV, p-AnV, TOTOX, TBA and FFA values of groundnut oil were 10.31 meq O2/kg, 4.39, 25.01, 0.99 and 1.49% respectively; color 1.40R, 22.00Y and induction time (IT) 5.33 h. After 90 days storage at room temperature, the PV, pAn -V, TOTOX, TBA and FFA values were respectively ranged between 10.31-21.32 meq O2/kg, 4.39-7.36, 25.01-50.12, 0.99-2.42 ppm and 1.49-2.64%; the color in the red and yellow unit 1.40-1.20R and 22.00-19.00Y respectively; and the induction time 5.33-3.77 h. However, the same parameters at the end of exposure to sunlight were ranged in the same order, between 10.31-43.47 meq O2/kg, 4.39-22.24, 25.01-109.18, 0.99-3.25 ppm and 1.49-5.83% respectively; the color in the red and yellow units 1.40-0.50R and 22.00-1.30Y respectively; and the induction time 5.33-0.03 h. Results also showed that the amount of linoleic and gadoleic acids significantly dropped under sunlight compared to dark at room temperature. Sunlight significantly reduced the quality of groundnut oil. This oil should be stored in the dark at room temperature.
Cameroon Journal of Experimental Biology, Mar 10, 2021
The current study was conducted in four localities in the West region of Cameroon from May to Sep... more The current study was conducted in four localities in the West region of Cameroon from May to September 2019, to know the pre-treatments made on given to beef hides before their use in different dishes, as well as the ingredients used in the preparation of pepper soup beef hides through household surveys. A total of 307 housewives were surveyed interviewed. The data was collected using a questionnaire and further analyzed using Sphinx Plus2 V5 software. Results showed that 54.6 to 71% participants consumed beef hides monthly. More than 50% participants purchased their beef hides at the butchery at a price varying between XAF 1,000 for 1Kg of uncleaned smoked beef hides and XAF 3,000 for 2Kg of clean smoked beef hides. The results also showed that there were five main stages in the preparation of beef hides and housewives had a greater preference of beef hides from the head and buttocks. The pre-cooking of beef hides was done between 180 and 240 minutes, with a quantity of water varying between 4 and 5 liters for 1 to 2Kg of beef hides, on the wood fire. “Eru” and “Yellow achu sauce” were cited as the main sauces in which beef hides were used at 95.40% and 85% respectively. Besides, more than 70% of housewives used a maximum of five ingredients for the preparation of pepper soup beef hides. Our findings revealed, interesting insights on the steps and ingredients used when preparing pepper soup beef hides that will be of great importance in the development of better formulation for pepper soup beef hides preparation
The effects of different processing methods on proximate composition, total phenolic content, ant... more The effects of different processing methods on proximate composition, total phenolic content, antioxidant activity, lipid oxidation, and mineral contents of sunflower seeds produced in Far North Region of Cameroon were evaluated. Mean moisture, ash, lipid, protein, fiber, and carbohydrate contents of raw sunflower seeds were 6.60%, 2.55%, 44.65%, 20.17%, 4.08%, and 21.25%, respectively. The changes in moisture, ash (excepted in boiled samples), lipid, protein, fiber, and carbohydrate (excepted in roasted samples) were found to be significant for all cooking methods. Ash and lipid contents of samples roasted at 120°C were found to be significant when compared with other cooking methods. Antioxidant activity increased with treatment. After processing, the acid, peroxide, and thiobarbituric acid values increased significantly, whereas iodine value decreased. The roasting process improved the induction time, and samples roasted at 120°C were found to have the highest induction time (2.29 ± 0.09 hr). Raw sunflower seeds were good sources of potassium (K), magnesium (Mg), calcium (Ca), iron (Fe), zinc (Zn), and manganese (Mn). Increase in contents of Ca, Mg, Zn, Cu, and Fe was observed during processing. Roasting compared with boiling appeared to be the best cooking method of sunflower seeds concerning nutrient content, antioxidant stability, and lipid stability.
Population Medicine considers the following types of articles: • Research Papers-reports of data ... more Population Medicine considers the following types of articles: • Research Papers-reports of data from original research or secondary dataset analyses. • Review Papers-comprehensive, authoritative, reviews within the journal's scope. These include both systematic reviews and narrative reviews. • Short Reports-brief reports of data from original research. • Policy Case Studies-brief articles on policy development at a regional or national level. • Study Protocols-articles describing a research protocol of a study. • Methodology Papers-papers that present different methodological approaches that can be used to investigate problems in a relevant scientific field and to encourage innovation. • Methodology Papers-papers that present different methodological approaches that can be used to investigate problems in a relevant scientific field and to encourage innovation. • Letters to the Editor-a response to authors of an original publication, or a very small article that may be relevant to readers. • Editorials-articles written by the Editorial Board or by invited experts on a specific topic. Research Papers Articles reporting research may be full length or brief reports. These should report original research findings within the journal's scope. Papers should generally be a maximum of 4000 words in length, excluding tables, references, and abstract and key points of the article, whilst it is recommended that the number of references should not exceed 36.
Population Medicine considers the following types of articles: • Research Papers-reports of data ... more Population Medicine considers the following types of articles: • Research Papers-reports of data from original research or secondary dataset analyses. • Review Papers-comprehensive, authoritative, reviews within the journal's scope. These include both systematic reviews and narrative reviews. • Short Reports-brief reports of data from original research. • Policy Case Studies-brief articles on policy development at a regional or national level. • Study Protocols-articles describing a research protocol of a study. • Methodology Papers-papers that present different methodological approaches that can be used to investigate problems in a relevant scientific field and to encourage innovation. • Methodology Papers-papers that present different methodological approaches that can be used to investigate problems in a relevant scientific field and to encourage innovation. • Letters to the Editor-a response to authors of an original publication, or a very small article that may be relevant to readers. • Editorials-articles written by the Editorial Board or by invited experts on a specific topic. Research Papers Articles reporting research may be full length or brief reports. These should report original research findings within the journal's scope. Papers should generally be a maximum of 4000 words in length, excluding tables, references, and abstract and key points of the article, whilst it is recommended that the number of references should not exceed 36.
Annals of the University "Dunărea de Jos" of Galaţi, Mar 29, 2022
This work aimed to assess the oxidative stability of cottonseed oil enriched with natural antioxi... more This work aimed to assess the oxidative stability of cottonseed oil enriched with natural antioxidants from Cameroonian plant leaves extracts. Total polyphenols, flavonoids, and antioxidant activity via DPPH radical scavenging assay were assessed in ethanolic extracts. These extracts were added to cottonseed oil at concentrations of 200, 500, 1250, and 2000 ppm. The cottonseeds' oil oxidative stability was evaluated by determining their induction/stability times on Rancimat (120±1.6°C) and by measuring their oxidative state in Schaal oven during 30 days (samples were removed after 10 days) of storage at 60°C. The outcomes showed that Canarium schweinfurthii, Trichoscypha acuminate, Dacryodes edulis, and Psidium guajava leaves extracts are rich in polyphenols (18.56 to 42.70 mg/g) and flavonoids (2.14 to 5.36 mg/g). The ethanolic extracts, above 1250 ppm, were also efficient like synthetic antioxidants in inhibiting the oxidation of cottonseeds oil at high temperatures. The evaluation of oxidation parameters of enriched cottonseeds oil showed that different extracts at 2000 ppm can limit the appearance of both primary and secondary oxidation products in oils.
Soya bean (variety TGX 1835-10E) is a legume commonly produced and consumed in Cameroon. Despite ... more Soya bean (variety TGX 1835-10E) is a legume commonly produced and consumed in Cameroon. Despite its affordability compared to animal sources of proteins, protein energy malnutrition (PEM) is still observed in the country and especially in rural areas. This can be attributed the way the available soya bean is processed which can easily affect its physicochemical properties and reduce its nutritional value and functional properties. This study was conducted in order to evaluate the effect of different processing methods on the nutritional composition, phytochemical and functional properties of soya bean. The beans were divided into nine groups that were processed differently and analyzed for their total phenolic content, antioxidant activity, oil quality and nutritional composition and the functional properties of their flours. Results showed that the total phenolic content was found to be ranged between 99.84 - 216.85 mg GAE/g and significantly increased with roasting and decreased ...
e present study aimed at determining the optimal conditions for extraction of total phenolic comp... more e present study aimed at determining the optimal conditions for extraction of total phenolic compounds from soybean (Glycine max) meal, a by-product of the soybean seeds industry, using a green protocol with microwave-assisted extraction (MAE). A facecentered composite design (FCCD) was used for optimization. Based on a screening aimed to determine the factors that significantly influenced the responses, a 50% hydro-ethanolic solution was used with solvent/dry matter ratio (60/1-110/1), power (120-270 W), and time (0-10 min) as factors, while the responses studied were total phenolic and flavonoid contents. FTIR, TLC, DPPH, and FRAP anti-oxidants tests were used to characterize the extracts obtained with optimum conditions. e factors that significantly influenced both responses were the individual effect of all factors, the interaction between solvent/dry matter ratio and extraction time, the quadratic effect of solvent/dry matter ratio, and power for total phenolic content, while only the quadratic effect of power significantly influenced the flavonoid content. e highest contents of phenols (13.09 mg GAE/g) and flavonoid (7.39 mg CE/g) were obtained at 120 W for 0.16 min with a solvent/dry matter ratio of 60/1. ATR-FTIR spectra indicated the presence of polyphenolic compounds in the extract, namely flavonoids. TLC indicated the presence of at least nine compounds in the extract, among which catechin and quercetin were identified with respective Rf of 0.98 and 0.93. DPPH assay showed the antioxidant capacity for the extract with an IC 50 of 194.98 µg/ml. RSM permitted us to develop a green protocol for maximum extraction of polyphenols from soybean seeds waste using less solvent, low power, and a reduced time in MAE.
International Journal of Biological and Chemical Sciences, Aug 17, 2020
Fish with higher nutrient qualities can be used to solve malnutrition problems in Africa. So the ... more Fish with higher nutrient qualities can be used to solve malnutrition problems in Africa. So the aim of this study was to evaluate the effect of local smoking on proximate composition and lipid oxidation of Chrysichthys nigrodigitatus collected in Cameroon. The proximate composition was assessed using standard methods while lipid quality assessment was based on the determination of biochemical indexes. A change on fatty acid composition was evaluated by gas chromatography while minerals composition was evaluated by spectrophotometer. On nutritional point of view, raw fish have high protein, lipid and ash contents (51.87, 37.48 and 8.55 g/100 g dry weight respectively). Unsaturated fatty acid may be up to 57% of the total fatty acids. Raw C. nigrodigitatus contain reasonable amount of minerals. Smoking process increased ash and lipid content while proteins and moisture contents are reduced in these fish. The iodine value of C. nigrodigitatus was reduced while acid, peroxide and TOTOX values increased during smoking. The marked decrease of polyunsaturated fatty acids was noted during processing. Mineral contents were affected by smoking process. Calcium, magnesium, potassium, sodium, manganese and iron contents increased significantly. In conclusion, hot smoking enhances nutrients content of C. nigrodigitatus while significant reduction in lipid quality was recorded.
European Food Research and Technology, Dec 11, 2017
The objective of this study was to evaluate the effect of ginger roots methanolic extract on the ... more The objective of this study was to evaluate the effect of ginger roots methanolic extract on the stability of palm olein during accelerated storage. After analysis of the extract by the measurement of its total phenolic content by colorimetry and detection of some of its phenolic antioxidants by HPLC-DAD (high performance liquid chromatography-diode array detector) and ESI-MS (Electrospray ionization-mass spectrometer), preliminary antioxidants tests have been conducted. After these tests, the extract has been added in refined palm olein at concentration 200-1800 ppm for investigating its effect on it oxidative stability. Butylated hydroxytoluene (BHT) at it legal concentration of 200 ppm served as positive control, while palm olein without additive served as negative control. Induction time, chemical indexes (peroxide, p-Anisidine, total oxidation, thiobarbituric acid and iodine values) and changes in linoleic acid profile (analyzed by gas chromatography coupled to a flame ionization detector) of oil samples were evaluated. Results of these investigations showed ginger roots methanolic extract to be rich in phenolic antioxidants. Ferulic acid and 6-gingerol were the antioxidants detected. The extract was also efficient in reducing palm olein oxidation on Rancimat and Schaal oven test of 30 days at 70 °C. Ginger roots extract might be a viable source of natural antioxidants for stabilization of palm olein.
The energy density of the complementary gruels can be increased by the use of sprouted flours. Th... more The energy density of the complementary gruels can be increased by the use of sprouted flours. This led us to determine the conditions for obtaining and using sprouted corn flour for optimal fluidification of fermented cassava flour gruels. To do this, the germinated corn flour (GCF) varieties Atp and Kassaï was produced according to the response surface methodology and the Doehlert plan was used with factors such as soaking (12-48 h) and germination (24-96 h) times. Once obtained, these GCF were used to increase the energy density of cassava flour gruels with flow velocity as response. The Doehlert plan was also used with fermented cassava flour concentration (17.5-30 %) and optimised sprouted corn flour mass (1-5 g) as factors. The experimental design that had been performed indicated that the effectiveness of corn amylase flour to fluidify the gruels depends not only to the conditions of production (soaking and germination) (p < 0.05) but also their incorporation during the preparation (corn amylase-fermented cassava flour) (p < 0.05). The rich corn amylase flour can be obtained by soaking Kassaï and Atp varieties for 20 h and 28 h respectively and germinated for 90.82 h. Application of these GCF during the preparation of fermented cassava flour gruels has shown that to obtain gruels with flow velocities between 100-160 mm/30s, it was necessary to couple 1.16 g of sprouted corn flour variety Kassaï for 26.23 % of fermented cassava flour and 1.12 g of corn flour variety Atp for 25.94 % of fermented cassava flour. The use of these couples has made it possible to multiply the energy density of the gruels by 6.55 and 6.49, respectively. In view of these results, it is therefore advisable to use the germinated corn flours produced under the conditions obtained to fluidise and increase the energy density of the fermented cassava gruels.
The effects of refrigeration at 4 °C during 9 days on the quality and stability of catfish oil we... more The effects of refrigeration at 4 °C during 9 days on the quality and stability of catfish oil were evaluated using a change in fatty acid composition by gas chromatography (GC), commonly used analytical indexes (acid and peroxide values), and analysis by Fourier transform infrared (FTIR) spectroscopy. The results revealed that lipid deterioration, hydrolysis and oxidation occurred throughout the cold storage (4 °C). Refrigeration induced the lipolysis of triglycerides by lipases and phospholipases. It also affected the fatty acids composition of the catfish. The progressive loss of unsaturation was monitored by the decrease in the absorbance band at 3012 cm-1 on FTIR spectra and the lowest value was observed in the catfish muscle at 9 days of refrigeration. Eicosapentaenoic C20:5ω3 (EPA) and docosahexaenoic C22:6ω3 (DHA) acids were the polyunsaturated fatty acids most affected during refrigeration. Refrigeration for less than 5 days was found to be the best conditions for the preservation of the catfish.
This study investigates the effect of soursop flowers methanolic extract on the physicochemical c... more This study investigates the effect of soursop flowers methanolic extract on the physicochemical characteristics of palm olein during accelerated storage. After analysis of the extract by determining its total phenolic content by colorimetry and identification of some of its phenolic antioxidants by HPLC-DAD(Diode Array Detector), preliminary antioxidants tests have been done. After that, the extract was added in palm olein at concentrations 200-1800 ppm. BHT, at 200 ppm served as standard besides the Control. Induction time, peroxide, p-anisidine, Total oxidation (TOTOX), thiobarbituric acid and iodine values, as well as changes in linoleic acid profile Gas Chromatography/Flamme Ionization Detector (GC/FID) of oil during the storage were evaluated. Results showed soursop flower extract to be rich in phenolic antioxidants and to be efficient, at all concentrations, in delaying palm olein oxidation on Rancimat and accelerated Schaal oven test of 30 days at 70°C. Soursop flowers might be used as potent source of antioxidants for the stabilization of palm olein.
Antioxidant and antidiabetic activities of a polyphenol rich extract obtained from Abelmoschus es... more Antioxidant and antidiabetic activities of a polyphenol rich extract obtained from Abelmoschus esculentus (okra) seeds using optimized conditions in microwave-assisted extraction (MAE). Front. Nutr. :. doi: .
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Papers by Hilaire Womeni