Papers by Jun-Ichiro Hayashi
Object recognition supported by user interaction for service robots
We are researching about an age and gender estimation based on wrinkle texture and color of facia... more We are researching about an age and gender estimation based on wrinkle texture and color of facial images. Preliminary questionnaire (enquete) examination of how effectively the facial images could be used for gender and age estimations was executed by using 300 different faces and 21 examinees. Wrinkles appeared in the face and the shape and size of the facial parts
International journal of molecular sciences, Jan 13, 2017
Antimicrobial photodynamic therapy (aPDT) has been proposed as an adjunctive strategy for periodo... more Antimicrobial photodynamic therapy (aPDT) has been proposed as an adjunctive strategy for periodontitis treatments. However, use of aPDT for periodontal treatment is complicated by the difficulty in accessing morphologically complex lesions such as furcation involvement, which the irradiation beam (which is targeted parallel to the tooth axis into the periodontal pocket) cannot access directly. The aim of this study was to validate a modified aPDT method that photosensitizes indocyanine green-loaded nanospheres through the gingivae from outside the pocket using a diode laser. To establish this trans-gingival irradiation method, we built an in vitro aPDT model using a substitution for gingivae. Irradiation conditions and the cooling method were optimized before the bactericidal effects on Porphyromonas gingivalis were investigated. The permeable energy through the gingival model at irradiation conditions of 2 W output power in a 50% duty cycle was comparable with the transmitted ener...
Genome Announcements, 2015
Parascardovia denticolens JCM 12538 T was isolated from human dental caries. Here, we report the ... more Parascardovia denticolens JCM 12538 T was isolated from human dental caries. Here, we report the complete genome sequence of this organism. This paper is the first report demonstrating the completely sequenced and assembled genome of P. denticolens.
Genome Announcements, 2015
Scardovia inopinata JCM 12537 T was isolated from human dental caries. Here, we report the comple... more Scardovia inopinata JCM 12537 T was isolated from human dental caries. Here, we report the complete genome sequence of this organism. This paper is the first report to demonstrate the fully sequenced and completely annotated genome of an S. inopinata strain.
Genome announcements, Jan 9, 2015
Bifidobacterium dentium strain JCM 1195(T) was isolated from human dental caries. Here, we report... more Bifidobacterium dentium strain JCM 1195(T) was isolated from human dental caries. Here, we report the complete genome sequence of this organism.
Archives of oral biology, 2014
Previous studies have indicated that type-1 and type-2 interleukin-1 (IL-1) receptors (IL-1R1 and... more Previous studies have indicated that type-1 and type-2 interleukin-1 (IL-1) receptors (IL-1R1 and IL-1R2) play important roles in periodontitis progression. We investigated the association between periodontitis and polymorphisms in the IL-1R1 and IL-1R2 genes (IL1R1 and IL1R2). We searched for genetic variants in IL1R1 and IL1R2 in 24 Japanese patients with aggressive periodontitis (AgP) and 24 periodontally healthy controls. Thirty-eight single nucleotide polymorphisms (SNPs) were identified within genomic regions containing all exons and relevant exon-intron boundaries in IL1R1 and IL1R2. Possible associations of each gene locus with AgP were investigated in 119 AgP patients and 102 periodontally healthy controls using allelotypes, genotypes, and haplotypes. Significant differences were noted in the frequencies of 3 SNPs in IL1R2 (rs3819370, rs3218974 and rs3218977) for AgPs and controls (p=0.012, p=0.008, and p=0.038, respectively), after adjustment for gender and smoking status ...
Nihon saikingaku zasshi. Japanese journal of bacteriology, 1998
2008 Conference on Human System Interactions, 2008
For long years, image processing researchers pay tremendous effort to develop visual inspection s... more For long years, image processing researchers pay tremendous effort to develop visual inspection systems. To develop the visual inspection systems requires long and severe effort of image processing experts. But there is no method to evaluate the properness of the visual inspection system. In this paper, an attempt to evaluate the properness of the visual inspection system has been introduced. Also, human operators can detect the defects, very easily. Here, the visual inspection system with human operatorpsilas sensitivity simulator is discussed. To develop the simulator, the human sensitivity model for visual inspection should be accomplished. To have the model, several experiments to evaluate the human sensitivity have been required. In this paper, some experiences to evaluate the human sensitivity have been introduced, also.
Microbiology and Immunology, 1999
Bacterial binding phenomena among different bacterial genera or species play an important role in... more Bacterial binding phenomena among different bacterial genera or species play an important role in bacterial colonization in a mixed microbiota such as in the human oral cavity. The coaggregation reaction between two gram-negative anaerobes, Treponema medium and Porphyromonas gingivalis, was characterized using fimbria-deficient mutants of P. gingivalis and specific antisera against purified fimbriae and bacterial whole cells. T. medium ATCC 700273 strongly coaggregated with fimbriate P. gingivalis strains ATCC 33277 and 381, but not with afimbriate strains including transposon-induced fimbria-deficient mutants and KDP98 as a fimA-disrupted mutant of P. gingivalis ATCC 33277. In the P. gingivalis-T. medium coaggregation assay, the presence of rabbit antiserum against the purified fimbriae or the whole cells of P. gingivalis ATCC 33277 produced different "aggregates" consisting predominantly of P. gingivalis cells with few spirochetes, but both preimmune serum and the antiserum against the afimbriate KDP98 cells did not inhibit the coaggregation reaction. Heated P. gingivalis cells lost their ability to bind both heated and unheated T. medium cells. This T. medium-P. gingivalis coaggregation reaction was inhibited by a cysteine proteinase inhibitor, leupeptin, and also by arginine and lysine, but not by EDTA or sugars including lactose. A binding assay on nitrocellulose membranes and immunoelectron microscopy demonstrated that a heat-stable 37 kDa surface protein on the T. medium cell attached to the P. gingivalis fimbriae.
Microbiology and Immunology, 2000
ABSTRACT Porphyromonas gingivalis, a periodontopathogen, is an oral anaerobic gram-negative bacte... more ABSTRACT Porphyromonas gingivalis, a periodontopathogen, is an oral anaerobic gram-negative bacterium with numerous fimbriae on the cell surface. Fimbriae have been considered to be an important virulence factor in this organism. We analyzed the genomic DNA of transposon-induced, fimbria-deficient mutants derived from ATCC 33277 and found that seven independent mutants had transposon insertions within the same restriction fragment. Cloning and sequencing of the disrupted region from one of the mutants revealed two adjacent open reading frames (ORFs) which seemed to encode a two-component signal transduction system. We also found that six of the mutants had insertions in a gene, fimS, a homologue of the genes encoding sensor kinase, and that the insertion in the remaining one disrupted the gene immediately downstream, fimR, a homologue of the response regulator genes in other bacteria. These findings suggest that this two-component regulatory system is involved in fimbriation of P. gingivalis.
Nihon Shishubyo Gakkai Kaishi (Journal of the Japanese Society of Periodontology), 2008
：The smoking prevalence, social nicotine dependence and efficacy of anti-smoking education among ... more ：The smoking prevalence, social nicotine dependence and efficacy of anti-smoking education among 40 dental hygienists aged 21 to 57 years (36.1 (SD10.5) years) old working at a dental hospital and their families were studied using the Kano Test for Social Nicotine Dependence (KTSND). The KTSND has 10 questions with a total score of 30. The questionnaire was administered at baseline, after an initial anti-smoking education program, before and after an additional anti-smoking education program at 6 months from the baseline, and finally at 13 months after the baseline. Twenty-six of the 40 dental hygienists (37.5 (10.7) years) responded to all the five questionnaires. The subject population was composed of one ex-smoker (3.8%), 25 subjects who had never smoked (96.2%), and 9 (32.5%) subjects who inhaled secondhand smoke at home. The total KTSND score of 8.6 (5.1) decreased significantly to 3.5 (4.4) after the initial anti-smoking education program, 連絡先：竹内あゆ美 〒 464-8651 愛知県名古屋市千種区末盛通り 2-11
Nihon Shishubyo Gakkai Kaishi (Journal of the Japanese Society of Periodontology), 2004
Estrogen is thought to play a role in inhibiting bone resorption and reduced estrogen levels corr... more Estrogen is thought to play a role in inhibiting bone resorption and reduced estrogen levels correlate with postmenopausal osteoporosis. M enopause and oophorectomy have been associated with increased production of bone resorption-inducing cytokines. The purpose of the present study was to compare the concentrations of Interleukin-1 (IL-1 )in the gingival crevicular fluid(GCF)of premenopausal and postmenopausal women. IL-1α,IL-1β ,IL-1receptor antagonist(ra)and the total IL-1 /IL-1ra ratio(IL-1 activity index；IL-1AI)were measured in 14 4GCF samples from 7 2sites in 1 2premenopausal patients(Pr group：age 4 4 .2 ±2. 2 )and 72 sites in 1 2 postmenopausal patients(Po group：age 57 . 8±1. 3 ) . IL-1α, IL-1β and IL-1ra were measured by specific non-crossreactive enzyme linked immunosorbent assays. The probing depth, clinical attachment level, bleeding on probing, tooth mobility, gingival index, and alveolar bone loss of each site were recorded at the time of GCF sampling. The marginal gingival inflammation in both groups was well controlled and the clinical inflammatory assessments of the gingival condition were indistinguishable in both groups. The concentrations of IL-1α,IL-1β ,IL-1ra and IL-1AI in the Po group were significantly higher than those in the Pr group(IL-1α1 1 0. 0 ±1 5 .1pg/ ml；61 . 3 ±8 .8 pg/ ml,P＜0 . 0 1,IL-β27 . 3±4. 8pg/ ml；15 . 6±3 .4pg/ ml,P＜0 . 05 ,IL-1ra 77 . 2±13 . 6ng/ ml；39 . 9 ±4 .3 ng/ ml, P＜0 . 0 1, IL-1 AI, 2 7. 3 ±4 .8pg/ ml；1 5. 6 ±3 . 4pg/ ml, 8695 1＜0 .0 5 ). Conclusively, the increased 259
Infection and Immunity, 2014
ABSTRACTThe interleukin-1 receptor antagonist (IL-1Ra) binds to IL-1 receptors and inhibits IL-1 ... more ABSTRACTThe interleukin-1 receptor antagonist (IL-1Ra) binds to IL-1 receptors and inhibits IL-1 activity. However, it is not clear whether IL-1Ra plays a protective role in periodontal disease. This study was undertaken to compare experimental periodontitis induced byAggregatibacter actinomycetemcomitansin IL-1Ra knockout (KO) mice and wild-type (WT) mice. Computed tomography (CT) analysis and hematoxylin-and-eosin (H&E) and tartrate-resistant acid phosphatase (TRAP) staining were performed. In addition, osteoblasts were isolated; the mRNA expression of relevant genes was assessed by real-time quantitative PCR (qPCR); and calcification was detected by Alizarin Red staining. Infected IL-1Ra KO mice exhibited elevated (P, <0.05) levels of antibody againstA. actinomycetemcomitans, bone loss in furcation areas, and alveolar fenestrations. Moreover, protein for tumor necrosis factor alpha (TNF-α) and IL-6, mRNA for macrophage colony-stimulating factor (M-CSF), and receptor activator ...
Drug and Alcohol Dependence, 1997
Cocaine is often used while consuming ethanol despite evidence that this combination may enhance ... more Cocaine is often used while consuming ethanol despite evidence that this combination may enhance the toxicity of cocaine. In the present study, we examined the cardiovascular effects of ethanol (475 or 950 mg/kg, i.v.) alone and in combination with cocaine (5 mg/kg, i.v.) in conscious rats. Ethanol or cocaine administration produced a consistent pressor response but highly variable cardiac output and systemic vascular resistance responses. The hemodynamic response patterns in individual rats to either drug were similar and related within rats. After ethanol pretreatment, cocaine produced greater decreases in cardiac output. We have proposed that this pattern of responses may reflect a predisposition in individual rats to cocaine-induced cardiomyopathies and hypertension. Furthermore, these data suggest that ethanol administration elicits a similar pattern of hemodynamic responses as previously reported for cocaine or amphetamine administration or acute behavioral stress.
Chemical Physics Letters, 1996
Archives of Oral Biology, 2013
Interleukin-15 Periodontal diseases Epithelial cells Interferon-gamma a b s t r a c t Objective: ... more Interleukin-15 Periodontal diseases Epithelial cells Interferon-gamma a b s t r a c t Objective: Oral epithelial cells act not only as mechanical barriers but also as immunological barriers by producing various mediators such as cytokines. Since, in periodontal disease, limited information is available regarding the role of oral epithelial cell-derived cytokines on T cell activation, we investigated the responses of human T cells (Jurkat cell) to cytokines in KB cells (an oral epithelial cell line) that had been stimulated with Aggregatibacter actinomycetemcomitans lipopolysaccharide (LPS). Design: To evaluate T cell activation in response to the culture supernatant of KB cells, we examined cell proliferation and interferon gamma (IFN-g) production, which is closely related to periodontal disease, in Jurkat cells. Culture supernatant of LPS-stimulated KB cells enhanced cell proliferation and IFN-g production in Jurkat cells. To determine the active component within the culture supernatant, the production of epithelial cell-derived cytokines, interleukin-12 (IL-12), IL-15 and IL-18, in LPS-stimulated KB cells was analysed. Results: IL-15, but not IL-18, was significantly increased in the culture supernatant of LPSstimulated KB cells. Moreover, additional anti-IL-15 neutralizing antibody abolished culture supernatant-induced IFN-g expression in Jurkat cells. Conclusion: These results suggest that periodontal pathogens induce the production of IL-15 from epithelial cells, and leading the activation of T cells in periodontal lesions.
Journal of the Japan Institute of Energy, 2015
Steam reforming (SR) and partial oxidation (POx) of nascent volatiles (NV) generated from fast py... more Steam reforming (SR) and partial oxidation (POx) of nascent volatiles (NV) generated from fast pyrolysis of cedar wood chips in a two-stage reactor were studied numerically. A detailed chemical kinetic model (DCKM) consisting of more than 8000 elementary step-like reactions and more than 500 chemical species was used to simulate pyrolysis at 750 °C and reforming of the NV at 900 °C in the first and second stages, respectively. The molecular composition of the NV, which is one of the required boundary conditions for computations using the DCKM, was approximated based on analytical pyrolysis experiments. Global reactions accounting for the decomposition of the ill-defined portion of the NV and soot reforming were also tested to improve the model capabilities. The DCKM with the global reaction coupled with a plug-flow reactor model could fairly reproduce the experimentally observed trends for the effects of oxygen and steam partial pressures on the yields of major products such as hydrogen, carbon monoxide, and tar residual rate.
Fuel Processing Technology, 2015
Coal conversion including reforming of nascent tar over the char surface in a drop tube reactor (... more Coal conversion including reforming of nascent tar over the char surface in a drop tube reactor (DTR) was studied both experimentally and numerically. Victorian brown coal and char prepared from the same coal were co-fed into an atmospheric DTR. The effects of reaction temperature (973-1173 K), solid holdup (8.31×10-6-2.50×10-4), residence time (0-4.6 s for gas; 0-0.78 s for solid particles), and steam partial pressure (0-0.05 MPa) on the conversion characteristics were investigated. A 4-lump kinetic model consisting of tar, gases, char, and soot with 6 global reactions was developed based on the experimental results. The lumped kinetic model was integrated with a computational fluid dynamics (CFD) simulation using an Eulerian-Eulerian approach for mixed phase flow to simulate the coal conversion experiments
ISIJ International, 2014
A sequence of briquetting of biomass solids (bamboo, larch and mallee) at temperature and mechani... more A sequence of briquetting of biomass solids (bamboo, larch and mallee) at temperature and mechanical pressure of 130-200°C and 114 MPa, respectively, and carbonization at 900°C produces coke with tensile strength (TS) of 5-19 MPa. Introduction of heat treatment in hot-compressed water (i.e., hydrothermal treatment; HT) of the biomass prior to the briquetting increases TS up to 44, 57 and 42 MPa for the bamboo, larch and mallee, respectively. TS of coke is correlated well and positively with the coke/briquette bulk density ratio, and HT increases the ratio if operated under appropriate conditions. The efficacy of HT is attributed primarily to increase in the coke yield on a basis of the briquette mass. HT hydrolytically removes highly volatile cellulosic material (i.e., cellulose and hemicellulose), transforms it into solid that contributes to coke as effectively as lignin, and thereby increases the mass yield of coke by a factor of 1.4 to 2.1. HT also enhances the plasticizability of the biomass during the briquetting by degradation of the lignin to reasonable extent, and then promotes particles' coalescence/fusion and densification of the briquettes. Applying mechanical pressure over a range of 12-114 MPa to the briquetting of a solid from HT of the bamboo at 240°C successfully results in production of coke with TS of 41-44 MPa.
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Papers by Jun-Ichiro Hayashi