Papers by Harshad Brahmbhatt
International Journal of Environmental Engineering, Mar 20, 2015
The investigation was carried out to remediate simazine from water by the pressure driven filtrat... more The investigation was carried out to remediate simazine from water by the pressure driven filtration techniques (viz. nanofiltration, reverse osmosis). The separation of simazine through the membranes showed similar trend as glucose (organic marker) and salt. Surfactant-mediated filtration showed better performance of the membranes, though it depends on the nature of membranes and surfactants. The membranes having more porous (in terms of glucose and water permeability) structure showed improvement in the separation of simazine. The Sodium Dodecyl Sulphate (SDS) added water contaminated with simazine shows better separation with respect to Cetyl Trimethyl Ammonium Bromide (CTAB). Pure water Flux Recovery Ratio (FRR) is higher for the membranes dealt with SDS with respect to CTAB.
International Journal of Pharmaceutics, 2013
The organized mesoporous matrices with large surface area and large pore volumes are potential dr... more The organized mesoporous matrices with large surface area and large pore volumes are potential drug carriers and hence find good applications in the field of controlled and sustained drug delivery. Two novel mesoporous synthetic hectorite (MSH) materials, namely, MSH3 and MSH4 with diverse composition and pore performance have been synthesized by a template free route and studied for the controlled drug delivery applications. MSH3 with 0.14LiF:5.93 Mg(OH) 2 :8 SiO 2 synthetic composition exhibited higher quinine adsorption than that by MSH4 (2.8LiF:4.6 Mg(OH) 2 :8SiO 2 ). In vitro studies at 37 ± 0.5 • C temperature under sequential buffer conditions showed controlled drug release with respect to the variation in pH values while following Eudragit VR L100 coated gelatin capsules; however, dialysis bag technique do not show such pH controlled delivery. Kinetic data suggest the release of QUI from the nanocomposite follows dissolution diffusion model.
Asian Journal of Pharmaceutical Sciences, 2015
Tel.:+91 278 2562548; fax: +91 278 2567562 ADC 1:4 was prepared with CP and ADC-3 IER by ion exch... more Tel.:+91 278 2562548; fax: +91 278 2567562 ADC 1:4 was prepared with CP and ADC-3 IER by ion exchange complexing method for taste masking studies. DSC show the shifting of peak to lower temperature of ADC 1:4 compared to ADC-3 and CP indicates that CP might be amorphous by complexing with ADC-3 IER.
Journal of Applied Polymer Science, 2014
Some of the best entities for the removal of volatile organic pollutants from water, poly(dimethy... more Some of the best entities for the removal of volatile organic pollutants from water, poly(dimethyl siloxane) (PDMS) membranes from two types of initial PDMS precursors, were thoroughly investigated. In both the cases, the sizes of the initial macromonomers increased because of the crosslinking in the liquid state, and they exhibited Gaussian chain statistics, which were condensed by further crosslinking to the formation of the membranes. The structures of both the membrane types exhibited large interchain spacing on swelling; this implied a high degree of chain mobility. Their structural properties were corroborated by their separation performances.
Colloids and surfaces. B, Biointerfaces, 2014
This work reports intercalation of a sparingly soluble antibiotic (ciprofloxacin) into layered na... more This work reports intercalation of a sparingly soluble antibiotic (ciprofloxacin) into layered nanostructure silicate, montmorillonite (MMT) and its reaction with bone derived polypeptide, gelatin that yields three-dimensional composite hydrogel. Drug intercalation results in changes in MMT layered space and drug loaded MMT and gelatin creates 3D morphology with biodegradable composite hydrogels. These changes can be correlated with electrostatic interactions between the drug, MMT and the gelatin polypeptides as confirmed by X-ray diffraction patterns, thermal, spectroscopic analyses, computational modeling and 3D morphology revealed by SEM and TEM analysis. No significant changes in structural and functional properties of drug was found after intercalation in MMT layers and composite hydrogels. In vitro drug release profiles showed controlled release up to 150h. The drug loaded composite hydrogels were tested on lung cancer cells (A549) by MTT assay. The results of in vitro cell mi...
Separation Science and Technology, 2010
Plant Physiology and Biochemistry, 2011
< Method developed for simultaneous determination of major phytohormones. < Analysis time is subs... more < Method developed for simultaneous determination of major phytohormones. < Analysis time is substantially reduced. < Method evaluated for accuracy, precision, linearity and stability. < Phytohormones were further characterized with ESI-MS analysis.
Macromolecular Research, 2008
The pressure driven membrane process has been a breakthrough in the removal of pollutants from dr... more The pressure driven membrane process has been a breakthrough in the removal of pollutants from drinking water. These experiments examined the removal of chlorophenol compounds from water using low pressure membranes. The removal performance of the membranes was based primarily on size exclusion. Apart from size exclusion, the polarity and pKa of the compounds also influences the membrane performance. The
Journal of Agricultural and Food Chemistry, 2010
The sap expelled from the fresh harvest of Kappaphycus alvarezii, a red seaweed growing in tropic... more The sap expelled from the fresh harvest of Kappaphycus alvarezii, a red seaweed growing in tropical waters, has been reported to be a potent foliar spray. Tandem mass spectrometry of various organic extracts of the sap confirmed the presence of the plant growth regulators (PGRs) indole 3-acetic acid, gibberellin GA 3 , kinetin, and zeatin. These PGRs were quantified in fresh state and after 1 year of storage by ESI-MS without recourse to chromatographic separation. Quantification was validated against HPLC data. The results may be useful in correlating with the efficacy of the sap. The methodology was extended to two other seaweeds. The method developed is convenient and precise and may find application in other agricultural formulations containing these growth hormones.
European Journal of Pharmaceutics and Biopharmaceutics, 2012
We report here the intercalation of 5-fluorouracil (5-FU), an anticancer drug in interlayer galle... more We report here the intercalation of 5-fluorouracil (5-FU), an anticancer drug in interlayer gallery of Na + clay (Montmorillonite, MMT), with the assistance of biopolymer (chitosan, CS). The X-ray diffraction patterns, thermal and spectroscopic analyses indicated the drug intercalation into the clay interlayer space in support of CS and stabilized in the longitudinal monolayer by electrostatic interaction. In vitro drug release showed controlled release pattern. The genotoxic effect of drug was in vitro evaluated in human lymphocyte cell culture by comet assay, and results indicated significant reduction in DNA damage when drug was intercalated with clay and formulated in composites. The results of in vitro cell viability assay in cancer cells pointed at decreased toxicity of drug when encapsulated in Na + -clay plates than the pristine drug. In vivo pharmacokinetics, biodistribution, hepatotoxicity markers, e.g., SGPT and SGOT, and liver/ testicular histology in rats showed plasma/tissue drug levels were within therapeutic window as compared to pristine drug. Therefore, drug-clay hybrid and composites can be of considerable value in chemotherapy of cancer with reduced side effects.
Composites Science and Technology, 2014
This work evaluates cationic intercalation of Capecitabine (Xeloda), an antineoplastic agent in i... more This work evaluates cationic intercalation of Capecitabine (Xeloda), an antineoplastic agent in interlayer gallery of Na + -MMT, which was further compounded with Poly (L-Lactide) to form microcomposite spheres, for oral chemotherapy of breast cancer. The XRD, TGA, SEM, TEM and FT-IR analysis indicated the CAP intercalated in interlayer of MMT and stabilized in the longitudinal monolayer mode by electrostatic interface. No significant alteration in fundamental active properties of CAP was found in the MMT efficacy and data from in vitro study showed controlled drug release pattern >72 h. The efficacy of CAP-MMT hybrid/MPs was also confirmed on Human neuroblastoma cell line (IMR32) by in vitro cell culture experiments evaluating cell cytotoxic and oxidative stress marker indices which showed that intercalated drug and MPs, were as effective as pristine drug. In vivo pharmacokinetics of CAP-MMT hybrids in rats was examined and plasma CAP levels were evaluated. Our findings suggest that CAP-MMT hybrids and MPs can be of considerable value in chemotherapy of malignant neoplastic disease with reduced side effects and clearly demonstrated that MMT not only plays a role as a delivery matrix for drug but also facilitates significant increase in the delivery proficiency.
Bioresource Technology, 2012
The present study describes production of bio-ethanol from fresh red alga, Kappaphycus alvarezii.... more The present study describes production of bio-ethanol from fresh red alga, Kappaphycus alvarezii. It was crushed to expel sap -a biofertilizer -while residual biomass was saccharified at 100°C in 0.9 N H 2 SO 4 . The hydrolysate was repeatedly treated with additional granules to achieve desired reducing sugar concentration. The best yields for saccharification, inclusive of sugar loss in residue, were 26.2% and 30.6% (w/w) at laboratory (250 g) and bench (16 kg) scales, respectively. The hydrolysate was neutralized with lime and the filtrate was desalted by electrodialysis. Saccharomyces cerevisiae (NCIM 3523) was used for ethanol production from this non-traditional bio-resource. Fermentation at laboratory and bench scales converted ca. 80% of reducing sugar into ethanol in near quantitative selectivity. A petrol vehicle was successfully run with E10 gasoline made from the seaweed-based ethanol. Co-production of ethanol and bio-fertilizer from this seaweed may emerge as a promising alternative to land-based bio-ethanol.
Colloids and Surfaces B: Biointerfaces, 2013
Intercalation of 6-mercaptopurine (6-MP), an antineoplastic drug in interlayer gallery of Na + -c... more Intercalation of 6-mercaptopurine (6-MP), an antineoplastic drug in interlayer gallery of Na + -clay (MMT) was further entrapped in poly (l-lactide) matrix to form microcomposite spheres (MPs) in order to reduce the cell toxicity and enhance in vitro release and pharmacokinetic proficiency. The drug-clay hybrid was fabricated via intercalation by ion-exchange method to form MPs from hybrid. In vitro drug release showed controlled pattern, fitted to kinetic models suggested controlled exchange and partial diffusion through swollen matrix of clay inter layered gallery. The in vitro efficacy of formulated composites drug was tested in Human neuroblastoma cell line (IMR32) by various cell cytotoxic and oxidative stress marker indices. In vivo pharmacokinetics suggested that the intensity of formulated drug level in plasma was within remedial borders as compared to free drug. These clay based composites therefore have great potential of becoming a new dosage form of 6-MP.
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Papers by Harshad Brahmbhatt