Papers by Hengameh Dortaj
Journal of Biomedical Materials Research Part B: Applied Biomaterials, Dec 31, 2023
Research Square (Research Square), Jan 17, 2022
The administration of stem cells in regenerative medicine has emerged as a potential treatment op... more The administration of stem cells in regenerative medicine has emerged as a potential treatment option for many diseases. The privation of an impressive cure for joint pathologies such as knee osteoarthritis (OA) has increased concern in stem cell (SC)-based therapies. Many questions remain about the survival and biology of SC after transplantation. Current progression in molecular biology and imaging has accredited winsome non-invasive SC monitoring in living subjects. Over time, these methods have been updated to assess the viability and biology of SC transplantation. The use of nanoparticles has become important in many aspects of SCs tracking. Given that different tracking strategies are being used for clinical trials, it is important to choose the best one. In this review, various imaging methods, which were assimilated to monitor the viability and biology of SC after injection in osteoarthritis, were discussed. 1-Introduction In the early stages of osteoarthritis, cartilage destruction is not seen on a regular basis. However, as the disease progresses, joints are exposed to progressive in ammation and damage (1). The use of treatment methods for joint injuries, including drug therapy, orthopedic surgery, and arthroplasty, are costly and do not perfectly repair tissue (2, 3). There are some new technologies and approaches, such as regenerative medicine, to renovate the destruction of articular cartilage, which is commonly caused by trauma and osteoarthritis injuries that are increasingly prevalent, especially in the elderly population (4). Common cell-based treatments include culturing the patient's own cartilage, in which cells are removed from the individual, and then the chondrocyte cell is separated by enzymes and cultured outside the body to increase the number of cases (5, 6). It is then placed on an arti cial scaffold and is implanted in the body; the person's own healthy cartilage may also be used as a scaffold. However, adult chondrocytes are unable to reproduce extracellular matrices. Because cartilage tissue damage varies in-depth, physicians
Current Stem Cell Research & Therapy
: In the last decade, liver diseases with high mortality rates have become one of the most import... more : In the last decade, liver diseases with high mortality rates have become one of the most important health problems in the world. Organ transplantation is currently considered the most effective treatment for compensatory liver failure. An increasing number of patients and shortage of donors has led to the attention of reconstructive medicine methods researchers. The biggest challenge in the development of drugs effective in chronic liver disease is the lack of a suitable preclinical model that can mimic the microenvironment of liver problems. Organoid technology is a rapidly evolving field that enables researchers to reconstruct, evaluate, and manipulate intricate biological processes in vitro. These systems provide a biomimetic model for studying the intercellular interactions necessary for proper organ function and architecture in vivo. Liver organoids, formed by the self-assembly of hepatocytes, are microtissues and can exhibit specific liver characteristics for a long time in vitro. Hepatic organoids are identified as an impressive tool for evaluating potential cures and modeling liver diseases. Modeling various liver diseases, including tumors, fibrosis, non-alcoholic fatty liver, etc., allows the study of the effects of various drugs on these diseases in personalized medicine. Here, we summarize the literature relating to the hepatic stem cell microenvironment and the formation of liver Organoids.
Naunyn-schmiedebergs Archives of Pharmacology, Jul 22, 2023
Journal of Kerman University of Medical Sciences, May 1, 2021
Background: Corporation of Hyaluronic acid (HA) with PLGA is an effective way to potentially enha... more Background: Corporation of Hyaluronic acid (HA) with PLGA is an effective way to potentially enhance chondrogenesis. The aim of this study was to use HA macroporous biodegradable poly(lactic acid-co-glycolic acid) [PLGA] scaffold to enhance the attachment, proliferation and differentiation of chondrocytes for cartilage tissue engineering and articular cartilage regeneration of human adipose derived stem cells (hADSCs) in the presence of avocado/soybean unsaponifible (ASU). Methods: The PLGA and PLGA/HA scaffolds were prepared and hADSCs were cultured separately on the scaffolds and 14 days after differentiation, chondrogenic genes in each scaffold evaluated using real time PCR and cell viability examined by (3-(4,5-dimethylthiazol-2-yl)-2,5diphenyltetrazolium bromide (MTT) assay. Results: The viability and proliferation of cells in-group of PLGA significantly decreased in comparison with the control (P=0.002) and PLGA/HA (P=0.013) groups. The expression of (SOX9), Aggrecan (AGG), and Collagen type II (Col II) genes was significantly higher in the PLGA and PLGA/HA groups compared to the control group (P≥0.05). The gene expression of SOX9 (P=0.003) and AGG (P=0.009) was significantly higher in the PLGA/HA groups compared to the PLGA group. The results of real time PCR showed that collagen type X (Col X) gene expression in the PLGA group, was significantly higher than the control and PLGA/HA groups (P=0.000). Conclusion: The corporation of HA with PLGA is an effective way to potentially enhance chondrogenesis and articular cartilage regeneration of hADSCs in the presence of avocado/soybean unsaponifiables (ASU).
Traditional and integrative medicine, Oct 3, 2020
Rheumatic diseases such as osteoarthritis (OA), rheumatoid arthritis (RA), and low back pain are ... more Rheumatic diseases such as osteoarthritis (OA), rheumatoid arthritis (RA), and low back pain are very popular. The drugs available to treat these diseases are almost ineffective and have significant side effects. There are several approaches used to replace conventional drugs to treat these diseases. One of these methods is the use of herbal medicines. In this study, the effects of herbal medicines and medicinal plants used in the treatment of these diseases include. Searching for articles published in English from 1985 to 2020 using keywords include scientific and traditional names of plants reviewing Scopus and PubMed databases. There is limited research on the anti-rheumatic effects of these plants and the active ingredients. Therefore, further research is needed to determine the mechanism of action, the interaction of effects, the efficacy and safety of medicinal plants, and the potentially beneficial plant nutrients in treatment of these diseases seems necessary. The aim of this review was to update information on OA and chondrogenesis, also importance of herbal drugs for the management of arthritis.
Journal of Kerman University of Medical Sciences, Apr 25, 2023
Background: Sodium dodecyl sulfate (SDS) detergent is widely used in tissue decellularization to ... more Background: Sodium dodecyl sulfate (SDS) detergent is widely used in tissue decellularization to produce scaffolds for tissue engineering. Despite its strong decellularization, this substance has relatively high toxicity and causes changes in tissue composition. Sodium lauryl ether sulfate (SLES) is a new poly anionic detergent that is less toxic than SDS but weaker than it. The present study aimed to decellularize the intestinal tissue using SDS and SLES solutions, forming a cell scaffold, and examining scaffolds obtained from this tissue. Methods: Eighteen male Sprague-Dawley rats were divided into three groups. The intestines of all rats were removed after anesthesia. In the first group (controls), rats' intestines were placed in a 10% formalin solution. In the second group, intestines were decellularized using an SLES solution. In the third group animals' intestines were decellularized using an SDS solution. To evaluate decellularization, samples were stained with hematoxylin-eosin staining and Alcian blue staining for glycosaminoglycans (GAGs), and Masson's trichrome for collagen fibers. A confocal Raman microscope was used to compare collagen, lipid, GAG, and genetic content. Results: Hematoxylin-eosin staining showed that the nucleus and DNA were removed in the decellularized scaffolds by SDS or SLES. The SLES group, compared to the SDS group, showed fewer changes in the epithelial tissue, and muscle layers in both scaffolds were well preserved. The results of confocal Raman microscopy showed that tryptophan, lipid, glycogen, and protein were broken down by both detergents; however, the residual amount of glycogen was the same in both substances, but disulfide bonds of proteins, hydroxyproline, and lipids in the decellularized intestine with SLES were mostly preserved. Conclusion: Both substances were suitable for intestinal decellularization and removed the overall structure of intestinal tissue, but SLES retained collagen and GAG content better than SDS.
Journal of Materials Science: Materials in Medicine, Jun 18, 2022
Stem cells exist as normal cells in embryonic and adult tissues. In recent years, scientists have... more Stem cells exist as normal cells in embryonic and adult tissues. In recent years, scientists have spared efforts to determine the role of stem cells in treating many diseases. Stem cells can self-regenerate and transform into some somatic cells. They would also have a special position in the future in various clinical fields, drug discovery, and other scientific research. Accordingly, the detection of safe and low-cost methods to obtain such cells is one of the main objectives of research. Jaw, face, and mouth tissues are the rich sources of stem cells, which more accessible than other stem cells, so stem cell and tissue engineering treatments in dentistry have received much clinical attention in recent years. This review study examines three essential elements of tissue engineering in dentistry and clinical practice, including stem cells derived from the intra-and extra-oral sources, growth factors, and scaffolds.
Regenerative engineering and translational medicine, May 25, 2023
The administration of stem cells in regenerative medicine has emerged as a potential treatment op... more The administration of stem cells in regenerative medicine has emerged as a potential treatment option for many diseases. The privation of an impressive cure for joint pathologies such as knee osteoarthritis (OA) has increased concern in stem cell (SC)-based therapies. Many questions remain about the survival and biology of SC after transplantation. Current progression in molecular biology and imaging has accredited winsome non-invasive SC monitoring in living subjects. Over time, these methods have been updated to assess the viability and biology of SC transplantation. The use of nanoparticles has become important in many aspects of SCs tracking. Given that different tracking strategies are being used for clinical trials, it is important to choose the best one. In this review, various imaging methods, which were assimilated to monitor the viability and biology of SC after injection in osteoarthritis, were discussed. 1-Introduction In the early stages of osteoarthritis, cartilage destruction is not seen on a regular basis. However, as the disease progresses, joints are exposed to progressive in ammation and damage (1). The use of treatment methods for joint injuries, including drug therapy, orthopedic surgery, and arthroplasty, are costly and do not perfectly repair tissue (2, 3). There are some new technologies and approaches, such as regenerative medicine, to renovate the destruction of articular cartilage, which is commonly caused by trauma and osteoarthritis injuries that are increasingly prevalent, especially in the elderly population (4). Common cell-based treatments include culturing the patient's own cartilage, in which cells are removed from the individual, and then the chondrocyte cell is separated by enzymes and cultured outside the body to increase the number of cases (5, 6). It is then placed on an arti cial scaffold and is implanted in the body; the person's own healthy cartilage may also be used as a scaffold. However, adult chondrocytes are unable to reproduce extracellular matrices. Because cartilage tissue damage varies in-depth, physicians
Zygote, Nov 2, 2022
SummaryFertility preservation is one of the most important issues in assisted reproductive techno... more SummaryFertility preservation is one of the most important issues in assisted reproductive technology. Previous studies have shown that cytokines and growth factors can improve follicle growth. The endometrial stromal cells secrete various factors that are involved in maintaining the integrity of uterine and epithelial secretory function. The platelet-rich plasma contains a large assembly of platelets suspended in plasma that successfully improves the viability and growth of various cell lines. This work aimed to investigate the influences of conditioned medium (CM) and platelet-rich plasma (PRP) on the development of ovarian follicles in infertile mice due to cyclophosphamide (CYC) exposure. In this study, 65 healthy BALB/c female mice (∼28–30 g and 6-8 weeks old) in five groups were studied. Immunohistochemistry (IHC) was used to detect growth differentiation factor 9 (GDF9)-positive cells. The mRNA expression levels of SMAD1, SMAD2, and BMP15 was assessed using reverse transcription-polymerase chain reaction (RT-PCR) method. The expression levels of SMAD1, GDF9, BMP15, and SMAD2 in the CM+PRP group was significantly more than in the CM and PRP groups. In addition, live birth occurred in the CM+PRP group. Treatment with CM+PRP in infertile mice due to Cy exposure increased fertility and live-birth rate. In general, our study suggested that the CM and PRP combination could improve the growth of mice ovarian follicles in vivo.
PubMed, 2021
Due to the lack of suitable therapeutic approaches to cartilage defect, the objective of this stu... more Due to the lack of suitable therapeutic approaches to cartilage defect, the objective of this study was to determine the effect of Transforming growth factor-β3 (TGF-β3), avocado/soybean (ASU) and Kartogenin (KGN) on chondrogenic differentiation in human adipose-derived stem cells (hADSCs) on fibrin scaffold. hADSCs seeded in fibrin scaffold and cultured in chondrogenic media. These cells were divided into 4 groups (control, TGF-β3, ASU and KGN). Cell viability was estimated by MTT assay. Differentiated cells were evaluated by histological and immunohistochemical (IHC) techniques. Expression genes [sex determining region Y-box 9 (SOX9), Aggrecan (AGG), type II collagen (Coll II) and type X collagen (Coll X)] were assessed by real-time PCR. For a study on an animal model, differentiated cells in fibrin scaffolds were subcutaneously transplanted in rats. Histological and immunohistochemistry were done in the animal model. The results of the real-time PCR indicated that SOX9, AGG and Col II genes expression in TGF-β3, KGN and ASU groups were significantly higher (p < 0.01) compared to the control group, Col X gene expression only in the TGF-β3 group was significantly higher (p < 0.01) compared to the control group. The glycosaminoglycan (GAG) deposition was higher in TGF-β3, KGN and ASU groups compared to the control group. The immunohistological analysis showed the distribution of collagen type X in the extracellular matrix in the fibrin scaffold TGF-β3 group was significantly higher in control, KGN and ASU groups, and (p < 0.001). ASU, particularly KGN, was suitable for successful chondrogenic differentiation of hADSCs and a suppressor of the consequent hypertrophy.
Naunyn-schmiedebergs Archives of Pharmacology, Oct 31, 2020
Caffeine consumption increases during early adulthood, which has adverse effects on the reproduct... more Caffeine consumption increases during early adulthood, which has adverse effects on the reproductive system. This study aimed to assess the impact of embryonic caffeine exposure on rat ovary in adulthood. Female Wistar rats (240-270 g) were divided into 5 groups (n = 7): experimental groups were exposed to 26, 45, 100, and 150 mg/kg of caffeine via drinking water during pregnancy and the control group only received drinking water. The ovaries of the offspring were taken out on days 7, 14, 28, 60, 90, and 120 of postnatal development, and then, they were fixed in 10% formaldehyde solution. Ovarian follicles were studied using stereological methods, and data were analyzed using one-way ANOVA followed by the Tukey test in SPSS software. A value of p < 0.05 was considered significant. The body weight, the weight of the ovaries, the ovarian volume, and the number of primordial follicles decreased significantly (p < 0.05) in 45 and 100 mg/kg, and (p < 0.001) in 150 mg/kg caffeinetreated groups at all stages of postnatal development. Significant decreases were observed in the number of primary and secondary follicles in 45 and 100 mg/kg (p < 0.05) and (p < 0.001) in 150 mg/kg caffeine-treated groups on days 7, 14, 28, and 60 compared to the control group. The number of Graafian follicles also decreased significantly (p < 0.001) in 45, 100, and 150 mg/kg caffeine-treated groups on days 14 and 28. Moreover, the mean volume of the oocyte in Graafian follicles reduced considerably in 45, 100, and 150 mg/kg caffeine-treated groups compared to other groups (p < 0.05). The thickness of the zona pellucida (ZP) in the secondary follicles (p < 0.02) and Graafian follicles (p < 0.05) showed a significant reduction in 100 and 150 mg/kg caffeine-treated groups on the 14th, 28th, and 60th days. In conclusion, high-dose caffeine consumption during gestation affects all stages of ovarian follicle development in rat offspring.
Modern medical laboratory journal, 2020
Background and Objective: It is conceivable that caffeine consumption would induce gonadal change... more Background and Objective: It is conceivable that caffeine consumption would induce gonadal changes. The aim of this study is to assess the impact of embryonic caffeine exposure on rat testis and prostate. Materials and Methods: Female rats were divided into (n=7): A control, only received drinking water. B and C groups received caffeine low dose (26 mg/kg) and high dose (45 mg/kg) respectively via drinking water during pregnancy and lactation. Structural changes in testis and prostate were studied by using stereological methods at 21, 60 and120 days of postnatal development. Results: Our result showed decreases in body and testis weight of offspring of group C compared to other groups at all ages (P< 0.05). The Testis volume showed significant differences between the offspring of both experimental groups and control at days 21, 60 and 120 (P<0.05). There were significant differences in the number of sperm cells of offspring of experimental groups compared to the control group in different ages (P<0.05). The number of sertoli, spermatocyte and spermatid cells of offspring in group C showed a significant decrease compared with other groups at all days (P<0.01). The number of spermatogonia cells in group C offspring showed a significant decrease compared to the control group at different days (P<0.05). The mean Johnsen score decreased in offspring of group C compared to the control group (P<0.05). Conclusion: Results showed that maternal caffeine consumption altered the structure of testis and prostate gland and spermatogenesis of offspring in adulthood.
International journal of medical laboratory, Sep 5, 2020
Background and Aims: This study aimed to observe the effect of Icariin on histomorphometric chang... more Background and Aims: This study aimed to observe the effect of Icariin on histomorphometric changes of testis and prostate induced by Acrylamide. Materials and Methods: Male mice were divided into four groups (n=8): A is the control group and does not get any treatment, B is the sham group and only received drinking water. C group received Acrylamide 10 mg/kg. D group received Acrylamide 15 mg/kg+1.5 mg/kg of Icariin. Histological changes in testis and prostate were examined using stereological methods. Results: Results showed decreases in testis weight of the group treated by (p≤0.01) and the group cured by Acrylamide +Icariin group (p≤0.05). The total volume of testis showed a reduction in the Acrylamide group compared to other groups (p≤0.05). The total number of spermatogonia and spermatocyte cells in the Acrylamide group showed a decrease in comparison with the other groups (p≤0.05). The total number of spermatid cells in the Acrylamide group indicated a significant reduction in comparison with the control and sham group (p≤0.05). The total number of sertoli cells in the Acrylamide group showed a reduction when the number of leydig cells in the Acrylamide group showed a significant decrease in comparison with the control, sham, and Acrylamide+Icariin groups (p≤0.05). The mean Johnsen score was decreased in the Acrylamide treated group compared to control, sham, and Acrylamide+Icariin groups (p≤0.05). Testosterone concentration in the Acrylamide group showed a reduction in comparison with control, sham, and Acrylamide+Icariin groups (p≤0.05). Conclusions: Results demonstrated that Acrylamide altered the structure of the testis, prostate gland, and spermatogenesis stage, and Icariin treatment improved these histopathological changes.
International journal of medical laboratory, Mar 9, 2021
Suitable alternatives are made for damaged or diseased organs and tissues in tissue engineering b... more Suitable alternatives are made for damaged or diseased organs and tissues in tissue engineering by combining cellular and molecular biology with materials and mechanical engineering. Fibrin is a critical blood component responsible for homeostasis, used extensively as a biopolymer scaffold in tissue engineering. This study summarizes the latest developments in organ and tissue regeneration using fibrin as a scaffold material. The combination of active peptides and growth factors through a heparin-bound delivery system improves fibrin function as a scaffold. Besides, the development of fibrin precursors as recombinant proteins solves multiple or single donor fibrin adhesives. Its composite allows biomolecules to be combined with fibrin and can significantly enhance fibrin efficacy in cartilage tissue engineering applications.
Basic and clinical neuroscience, 2018
Introduction: Acrylamide (ACR) consumption is increasing all over the world. There are some evide... more Introduction: Acrylamide (ACR) consumption is increasing all over the world. There are some evidence on the literature about its neurotoxic effect on mature animals, but the effects of ACR on postnatal development have been less studied. The purpose of this study was to evaluate the effects of ACR on development of cortical layer, white matter, and number of Purkinje cells of the cerebellum in rat newborns. Methods: This study was carried out on 20 female Wistar rats (average weight: 180 g, aged: two months). The rats were divided into four groups. Pregnant rats were orally fed with ACR 10 mg/kg and vitamin C 200 mg/kg. In this study, 6 infants of each group (weighting 32-35 g) were randomly selected at day 21 after birth and placed under deep anesthesia and transcardial perfusion. Their cerebellums were fixed and histopathological changes were evaluated with Hematoxylin and Eosin (H&E) staining and cresyl violet method. The volume of cerebellar cortical layers and number of Purkinje cells were investigated by Cavalieri's principle and physical dissector methods. The obtained data were analyzed by 1-way ANOVA and LSD test using SPSS. P<0.05 considered as statistically significant. Results: The results showed that newborns of ACR-treated female rats have decreased cerebellar weight (P≤0.05) and lower than average number of Purkinje cells (P≤0.001). ACR also decreased the volume of granular and molecular layer and increased the volume of white matter. While the results showed decreased in white matter volume in vitamin C group (P≤0.001). Conclusion: ACR induces structural changes in the development of the cerebellar cortical layers in rat newborns, but these changes may be prevented by vitamin C as an antioxidant.
International journal of medical laboratory, Apr 3, 2022
Recently, it has been proven that cartilage healing is difficult. The most commonly used treatmen... more Recently, it has been proven that cartilage healing is difficult. The most commonly used treatments are autogenously cartilage grafting and allogeneic bone grafting, but grafts cannot fully meet treatment goals because of source, price, safety, and other concerns. Thus, a combination of biological materials and tissue engineering technology has become a recent trend in studies. Among the studies performed on tissue engineering cartilage materials are hydrogels that exhibit biological activity, post-decomposition adsorption, flexibility, and easy preparation. Cell-containing hydrogels are often used in cartilage tissue engineering because of their biocompatibility, ease of use, and ability to adapt to different defects. Hydrogels are used to mimic extracellular matrices. Although multiple materials can configure and form hydrogels, hyaluronic acid and its derivatives are distinguished. Hyaluronic acid (HA) is an extracellular molecule with several physical and biological functions found in many tissues, including cartilage. HA is formed in several biomaterial systems and scaffolding. HA hydrogels have many interests, including increased adhesion, cell proliferation, and wound healing. In addition, they represent adequate biological acting for stimulating a microenvironment for the survival of cells. However, their disadvantages include a slow degradation rate and low mechanical properties. Here, HA-based hydrogels and their applications in cartilage tissue engineering are briefly reviewed.
International journal of medical laboratory, Jun 2, 2021
Poly(lactic-co-glycolic) acid (PLGA) has attracted a considerable amount of interest for biomedic... more Poly(lactic-co-glycolic) acid (PLGA) has attracted a considerable amount of interest for biomedical application due to its biocompatibility, tailored biodegradation rate (depending on the molecular weight and copolymer ratio), approval for clinical use in humans by the U.S. Food and Drug Administration (FDA), the potential to change surface properties to create better interaction with biological materials and being suitable for export to countries and cultures where planting products with animals is unusable. For commercial use and research, PLGA has been widely studied to control small molecule drugs, proteins, and other macromolecules. This study aims to review the studies that used PLGA scaffolding and its composites as a scaffold and drug delivery in cartilage tissue engineering. It is concluded from the results that the PLGA scaffold as a synthetic scaffold, when combined with natural scaffolds or hybrids, strengthens its biological properties and performs its function better.
Journal of Research in Medical and Dental Science, 2018
Introduction: The potential toxicity of Acrylamide (ACR) in humans had become apparent with the d... more Introduction: The potential toxicity of Acrylamide (ACR) in humans had become apparent with the detection of this substance in some processed foods. ACR due to the cooking method are increasingly used. Aims: This study designed to investigate the effects of maternal ACR consumption on choroid plexus volume and capillaries and ventricular changes in rat embryonic. Methods: Female pregnant Wistar rats were divided into two groups; an experimental and the control group (n=10). Animals in control group received drinking water while rats in experimental group were orally administered 10 mg/kg ACR solution. Pregnant rats were sacrificed on the 15 th day of gestation. Their fetuses were taken out and after head fixation and tissue processing, serial sections were prepared and stained with haematoxylin-eosin (H & E). Choroid plexus and lateral ventricle volume and branches and length of the capillaries were measured and the results were analysed using SPSS version 21 and ANOVA statistical test. Results: Results showed a significant reduction in Choroid plexus volume and capillaries length in experimental group compared to control group (p˂0.05), the branches of capillaries showed significant decrease (p˂0.001). Volume of lateral ventricle showed increase in experimental group compared to control group (p˂0.001). Conclusion: Maternal ACR has toxic effect on the nervous system and induces structural changes in the development of choroid plexus and lateral ventricle.
Uploads
Papers by Hengameh Dortaj