Ipomoea fistulosa is an evergreen shrub with heart shaped leaves which is used for the treatment ... more Ipomoea fistulosa is an evergreen shrub with heart shaped leaves which is used for the treatment of various diseases in traditional medication. In this study, aqueous extract of I. fistulosa leaves was evaluated for phytochemical composition, antioxidant activity and cytotoxic activity by various in vitro methods. Phytochemical screening of the I. fistulosa leaves was carried out by using the standard protocols. Antioxidant potential of the aqueous extract was estimated by 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, metal chelating activity, total antioxidant activity and reducing power activity. Estimation of total phenolic content and flavonoid content of the aqueous extract of the plant was determined by using Folin- Ciocalteau reagent method and Aluminium chloride (AlCl3) method respectively. Cytotoxic activity of the plant extract was measured by Brine shrimp lethality assay. Preliminary phytochemical analysis of the aqueous extract of leaves of I. fistul...
Enzymes are the large biomolecules that are required for the numerous chemical interconversions t... more Enzymes are the large biomolecules that are required for the numerous chemical interconversions that sustain life. They accelerate all the metabolic processes in the body and carry out a specific task. Enzymes are highly efficient, which can increase reaction rates by 100 million to 10 billion times faster than any normal chemical reaction. Due to development in recombinant technology and protein engineering, enzymes have evolved as an important molecule that has been widely used in different industrial and therapeutical purposes. Microbial enzymes are currently acquiring much attention with rapid development of enzyme technology. Microbial enzymes are preferred due to their economic feasibility, high yields, consistency, ease of product modification and optimization, regular supply due to absence of seasonal fluctuations, rapid growth of microbes on inexpensive media, stability, and greater catalytic activity. Microbial enzymes play a major role in the diagnosis, treatment, biochemical investigation, and monitoring of various dreaded diseases. Amylase and lipase are two very important enzymes that have been vastly studied and have great importance in different industries and therapeutic industry. In this review, an approach has been made to highlight the importance of different enzymes with special emphasis on amylase and lipase in the different industrial and medical fields.
Ipomoea fistulosa is an evergreen shrub with heart shaped leaves which is used for the treatment ... more Ipomoea fistulosa is an evergreen shrub with heart shaped leaves which is used for the treatment of various diseases in traditional medication. In this study, aqueous extract of I. fistulosa leaves was evaluated for phytochemical composition, antioxidant activity and cytotoxic activity by various in vitro methods. Phytochemical screening of the I. fistulosa leaves was carried out by using the standard protocols. Antioxidant potential of the aqueous extract was estimated by 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, metal chelating activity, total antioxidant activity and reducing power activity. Estimation of total phenolic content and flavonoid content of the aqueous extract of the plant was determined by using Folin- Ciocalteau reagent method and Aluminium chloride (AlCl3) method respectively. Cytotoxic activity of the plant extract was measured by Brine shrimp lethality assay. Preliminary phytochemical analysis of the aqueous extract of leaves of I. fistul...
Enzymes are the large biomolecules that are required for the numerous chemical interconversions t... more Enzymes are the large biomolecules that are required for the numerous chemical interconversions that sustain life. They accelerate all the metabolic processes in the body and carry out a specific task. Enzymes are highly efficient, which can increase reaction rates by 100 million to 10 billion times faster than any normal chemical reaction. Due to development in recombinant technology and protein engineering, enzymes have evolved as an important molecule that has been widely used in different industrial and therapeutical purposes. Microbial enzymes are currently acquiring much attention with rapid development of enzyme technology. Microbial enzymes are preferred due to their economic feasibility, high yields, consistency, ease of product modification and optimization, regular supply due to absence of seasonal fluctuations, rapid growth of microbes on inexpensive media, stability, and greater catalytic activity. Microbial enzymes play a major role in the diagnosis, treatment, biochemical investigation, and monitoring of various dreaded diseases. Amylase and lipase are two very important enzymes that have been vastly studied and have great importance in different industries and therapeutic industry. In this review, an approach has been made to highlight the importance of different enzymes with special emphasis on amylase and lipase in the different industrial and medical fields.
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Papers by Neelam Gurung