Papers by Graciela Casado
Anales de la Fundación Alberto J Roemmers, 2014
Formato del Ensayo Placa 96 pocillos de media área Vol. de muestra (SP o LP) 2µl Vol. y tipo de B... more Formato del Ensayo Placa 96 pocillos de media área Vol. de muestra (SP o LP) 2µl Vol. y tipo de Buffer de dilución 20µl de Buffer Citrato/Fosfato 0,05M Vol. y especificaciones de sln. de sustrato 20µl de TMB 0,006% w/vol./ 0,003% H 2 O 2 en Buffer Citrato/Fosfato 0,05M Especificaciones de Sustrato elegido Polvo estéril, Sigma Solvente de reconstitución del sustrato Etanol 100% (Solución stock 0,3% TMB) Vol. de H 2 O 2 agregado a sln sustrato 2µl cada 10ml Tipo, vol. y concetración de sln. stop 20µl H 2 SO 4 2N
Frontiers in Cellular and Infection Microbiology, 2022
Alterations of myeloid cell populations have been reported in patients with tuberculosis (TB). In... more Alterations of myeloid cell populations have been reported in patients with tuberculosis (TB). In this work, we studied the relationship between myeloid-derived suppressor cells (MDSC) and monocytes subsets with the immunological responsiveness of TB patients. Individuals with active TB were classified as low responders (LR-TB) or high responders (HR-TB) according to their T cell responses against a cell lysate of Mycobacterium tuberculosis (Mtb-Ag). Thus, LR-TB, individuals with severe disease, display a weaker immune response to Mtb compare to HR-TB, subjects with strong immunity against the bacteria. We observed that LR-TB presented higher percentages of CD16 positive monocytes as compared to HR-TB and healthy donors. Moreover, monocyte-like (M-MDSC) and polymorphonuclear-like (PMN-MDSC) MDSC were increased in patients and the proportion of M-MDSC inversely correlated with IFN-γ levels released after Mtb-Ag stimulation in HR-TB. We also found that LR-TB displayed the highest perc...
Scientific Reports, 2020
Tuberculin skin test (TST) and IFN-γ release assays are currently used to detect Mycobacterium tu... more Tuberculin skin test (TST) and IFN-γ release assays are currently used to detect Mycobacterium tuberculosis (Mtb) infection but none of them differentiate active from latent infection (LTBI). Since improved tests to diagnose Mtb infection are required, we studied the immune response to Mtb latency antigen Rv2626c in individuals exposed to the bacteria during different periods. Tuberculosis patients (TB), TB close contacts (CC: subjects exposed to Mtb for less than three months) and healthcare workers (HW: individuals exposed to Mtb at least two years) were recruited and QuantiFERON (QFT) assay, TST and IFN-γ secretion to Rv2626c were analyzed. Twenty-two percent of the individuals assessed had discordant results between QFT and TST tests. Furthermore, QFT negative and QFT positive individuals produced differential levels of IFN-γ against Rv2626c, in direct association with their exposure period to Mtb. Actually, 91% of CC QFT negative subjects secreted low levels of IFN-γ to Rv2626c...
Alterations of myeloid cell populations have been reported in patients with tuberculosis (TB). Si... more Alterations of myeloid cell populations have been reported in patients with tuberculosis (TB). Since myeloid-derived suppressor cells (MDSC) and non-classical monocytes (CD14+CD16+) seem to play an important role in TB, we studied the relationship between these cells and the immunological status of patients. TB patients were classified as high responders (HR-TB) or low responders (LR-TB) according to their T cell responses against a whole cell lysate of Mycobacterium tuberculosis (Mtb-Ag). Thus, LR-TB, individuals with severe disease, display a weaker immune response to Mtb compare to HR-TB, subjects with strong immunity against the bacteria. We observed that LR-TB presented higher percentages of CD14+CD16+ monocytes as compared to HR-TB and healthy donors (HD). Moreover, monocyte-like (M-MDSC) and polymorphonuclear-like MDSC (PMN-MDSC) were increased in TB patients as compared to HD. Furthermore, the proportion of M-MDSC of TB patients inversely correlated with the levels of IFN-γ ...
Tubercle and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease, 1994
Patients with newly-diagnosed drug-sensitive, radiographically active and bacteriologically confi... more Patients with newly-diagnosed drug-sensitive, radiographically active and bacteriologically confirmed pulmonary tuberculosis recruited at 6 centres in Argentina, Brazil and Thailand. To assess the efficacy, tolerability and toxicity of two regimens containing different daily dosages of rifabutin in comparison with rifampicin. Multicentred, randomised, comparative study. In each group, study medications were administered daily for 6 months combined with isoniazid (6 months), and with pyrazinamide and ethambutol (both stopped after 2 months). Treatment success patients were followed-up for up to 2 years. A total of 520 patients were enrolled and randomly assigned to receive either rifampicin (n = 175), or rifabutin 150 mg (n = 174) or rifabutin 300 mg (n = 171). Considering all patients with positive baseline culture, the success rates at the last valid observation for each patient were 89%, 94% and 92% in the rifampicin, rifabutin 150 mg, and rifabutin 300 mg groups, respectively. Th...
Clinical Science, 2014
When B-cells from tuberculous pleural effusion are stimulated, they secrete interleukin-10, a pro... more When B-cells from tuberculous pleural effusion are stimulated, they secrete interleukin-10, a protein that regulates local inflammatory cells. This process, depending on its magnitude, may have a positive impact by limiting tissue damage or undesirable immunosuppressive effects.
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Papers by Graciela Casado