Then, experimental conditions have been defined to obtain mation of Ginkgo biloba by Agrobacteriu... more Then, experimental conditions have been defined to obtain mation of Ginkgo biloba by Agrobacterium tumefaciens. Emtransgenic kanamycin-resistant G. biloba calluses expressing bryos were co-cultivated with Agrobacterium tumefaciens GV3101 (pGV2260) carrying the binary vector pTHW136, GUS activity. The highest rate of transformation (45%) was which contained the gus reporter gene and the nptII selectable reached using 1.5-month-old embryos co-cultivated on a gene, encoding the enzymes -glucuronidase (GUS) and medium lacking mineral elements. The integration of gus and neomycin phophotransferase II, respectively. Transient GUS nptII genes in calluses was confirmed by polymerase chain reaction analysis and Southern blot analysis. activity has been used to screen the effects of different factors on the transfer of DNA into embryos (age of embryos, cells, the amounts of metabolite are too small for an industrial application. Gene-transfer technology offers new opportunities to improve the synthesis of plant secondary metabolites. Transgenic cells ) and hairy root ) have been obtained from the transformation of G. biloba with a wild strain of Agrobacterium rhizogenes. Opines were detected in these tissues, but their transgenic natures have not been confirmed by molecular assays. Moreover, the transformation frequencies were low. Terpenoid products have been analysed in transgenic cells . Although the amounts of ginkgolides were higher than the amounts measured in untransformed cell cultures, they remained lower than those present in the leaves of the tree. So far, there is no report on the transformation and ensuing modification of ginkgolide levels in G. biloba cells transformed by Agrobacterium tumefaciens. Such an experiment is reported in the present paper. With the aim of perfecting a valuable process for transfer-
The differentiation of the secretory cavities of Ginkgo stem and the structural organization of t... more The differentiation of the secretory cavities of Ginkgo stem and the structural organization of the epithelial cells were followed by light and electron microscopy. The mode of formation of the cavities is schizo-lysigeneous. Functional complexes of leucoplasts and associated ...
Then, experimental conditions have been defined to obtain mation of Ginkgo biloba by Agrobacteriu... more Then, experimental conditions have been defined to obtain mation of Ginkgo biloba by Agrobacterium tumefaciens. Emtransgenic kanamycin-resistant G. biloba calluses expressing bryos were co-cultivated with Agrobacterium tumefaciens GV3101 (pGV2260) carrying the binary vector pTHW136, GUS activity. The highest rate of transformation (45%) was which contained the gus reporter gene and the nptII selectable reached using 1.5-month-old embryos co-cultivated on a gene, encoding the enzymes -glucuronidase (GUS) and medium lacking mineral elements. The integration of gus and neomycin phophotransferase II, respectively. Transient GUS nptII genes in calluses was confirmed by polymerase chain reaction analysis and Southern blot analysis. activity has been used to screen the effects of different factors on the transfer of DNA into embryos (age of embryos, cells, the amounts of metabolite are too small for an industrial application. Gene-transfer technology offers new opportunities to improve the synthesis of plant secondary metabolites. Transgenic cells ) and hairy root ) have been obtained from the transformation of G. biloba with a wild strain of Agrobacterium rhizogenes. Opines were detected in these tissues, but their transgenic natures have not been confirmed by molecular assays. Moreover, the transformation frequencies were low. Terpenoid products have been analysed in transgenic cells . Although the amounts of ginkgolides were higher than the amounts measured in untransformed cell cultures, they remained lower than those present in the leaves of the tree. So far, there is no report on the transformation and ensuing modification of ginkgolide levels in G. biloba cells transformed by Agrobacterium tumefaciens. Such an experiment is reported in the present paper. With the aim of perfecting a valuable process for transfer-
The differentiation of the secretory cavities of Ginkgo stem and the structural organization of t... more The differentiation of the secretory cavities of Ginkgo stem and the structural organization of the epithelial cells were followed by light and electron microscopy. The mode of formation of the cavities is schizo-lysigeneous. Functional complexes of leucoplasts and associated ...
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