Papers by Gestter Willian Lattari Tessarin
Congresso de Extensão Universitária, 2011
Universidade Estadual Paulista (UNESP), Faculdade de Odontologia (FOA), Departamento de Materiais... more Universidade Estadual Paulista (UNESP), Faculdade de Odontologia (FOA), Departamento de Materiais Odontologicos e Protese, Aracatuba, SP, Brasil
PLOS ONE, Sep 19, 2017
Teneurins are transmembrane proteins consisting of four paralogues (Ten-1-4), notably expressed i... more Teneurins are transmembrane proteins consisting of four paralogues (Ten-1-4), notably expressed in the central nervous system during development. All teneurins contain a bioactive peptide in their carboxyl terminal named teneurin C-terminal associated peptide (TCAP). The present study analyzed the detailed distribution of teneurin-2-like immunoreactive (Ten-2-LI) cells in developing and mature rat molar teeth, as well as in mature human dental pulps. Ten-2 and TCAP-2 genic expressions were also evaluated in rat and human dental pulps. Finally, Ten-2-LI cells were analyzed during the repair process after dentinpulp complex injury in rat lower molar teeth. For this, histological sections of rat molar teeth and human dental pulps were submitted to immunohistochemical techniques, while total RNA from developing rat teeth and mature human dental pulps were submitted to conventional RT-PCR. Ten-2-LI cells were evident in the initial bell stage of rat molar teeth development, especially in ectomesenchymal cells of the dental papilla. Ten-2-LI odontoblasts showed strong immunoreactivity in rat and human mature teeth. Ten-2 and TCAP-2 genic expressions were confirmed in rat and human dental pulps. Dentin-pulp complex injury resulted in a decrease of Ten-2-LI odontoblasts after traumatic injury. Interestingly, Ten-2-LI cells were also evident in the pulp cell-rich zone in all postoperative days. In conclusion, Ten-2-LI presence in rat and human odontoblasts was demonstrated for the first time and Ten-2/TCAP-2 genic expressions were confirmed in rat and human dental pulps. Furthermore, it was revealed that Ten-2-LI rat odontoblasts can be modulated during the regenerative process.
European Journal of Histochemistry, Sep 15, 2016
The spinal cord is involved in local, ascending and descending neural pathways. Few studies analy... more The spinal cord is involved in local, ascending and descending neural pathways. Few studies analyzed the distribution of neuromediators in the laminae of non-human primates along all segments. The present study described the classic neuromediators in the spinal cord of the non-human primate Sapajus spp. through histochemical and immunohistochemical methods. Nicotinamide adenine dinucleotide hydrogen phosphate-diaphorase (NADPH-d) method showed neuronal somata in the intermediolateral column (IML), central cervical nucleus (
MedNEXT Journal of Medical and Health Sciences, Sep 30, 2022
Introduction: Aneurysmal bone cysts (ABC) are pseudocysts expansive lesions. The World Health Org... more Introduction: Aneurysmal bone cysts (ABC) are pseudocysts expansive lesions. The World Health Organization (WHO) categorizes primary ABCs as cystic neoplasms that contain fibrous septa separating numerous blood-filled spaces. Approximately 70% of ABC cases are primary, while 30% are secondary. Objective: The present study aimed to carry out a concise systematic review of the main clinical findings of aneurysmal bone cysts, highlighting the incidence and prevalence, as well as the main forms of treatment. Methods: The rules of the Systematic Review-PRISMA Platform were followed. The research was carried out from May 2022 to July 2022 and developed based on Scopus, PubMed, Science Direct, Scielo, and Google Scholar. The quality of the studies was based on the GRADE instrument and the risk of bias was analyzed according to the Cochrane instrument. Results: A total of 143 articles were found. In total, 73 articles were evaluated and 10 were included and evaluated in the systematic review. The symmetric funnel plot does not suggest a risk of bias. After prospecting the main literary findings, it was generally evidenced that the aneurysmal bone cyst (ABC) is a rare lesion that is more frequently found in young adults and children. It may have unpredictable behavior, with a high recurrence rate after treatment. Standard treatment consists of curettage plus local adjuvants and bone grafting to fill the void. In anatomic sites with difficult surgical access, percutaneous procedures are used (injection of sclerosing agents, radiofrequency thermal ablation, or selective arterial embolization. Medical management with bisphosphonates or denosumab has also been advocated. Conclusion: Primary ABCs involving the maxillofacial region are extremely rare and even more in patients with advanced age. Next-generation sequencing confirmed the presence of a USP6-CDH11 fusion gene, consistent with classification as a primary aneurysmal bone cyst. Sclerotherapy provided an effective and minimally invasive treatment for aneurysmal bone cysts and is particularly useful for deep injuries, challenging surgical access, and potentially injurious vital structures.
MedNEXT Journal of Medical and Health Sciences, Nov 4, 2021
Introduction: It is necessary to know the nature of the endodontic microbiota within the root can... more Introduction: It is necessary to know the nature of the endodontic microbiota within the root canal system of teeth with necrotic pulp tissues. There are several methods of microbial identification, including techniques based on culture or non-predominance of facultative anaerobes and Gram-positive species, especially Enterococcus faecalis. The 16S ribosomal RNA (rRNA) gene sequencing approach has become the reference method. Polymerase chain reaction (PCR) is a molecular technique also used. A condition for successful endodontic retreatment is proper cleaning of the root canals. Objective: Evaluate through a systematic literature review the main contaminations, recontaminations, and endodontic retreatments in root canals. Methods: The present study was followed by a systematic literature review model, according to the PRISMA rules. Clinical studies included case reports, retrospective, prospective and randomized trials. The quality of the studies was based on the GRADE instrument. The risk of bias was analyzed according to the Cochrane instrument. Results: A total of 94 articles were found. A total of 58 articles were evaluated in full, and 34 were included and discussed in this study. The overall assessment did not result in significant risks that could compromise the science of the present study. According to the GRADE classification, the studies were of moderate quality. Conclusion: It was concluded that it is essential to characterize the microbiota of root canals with failed endodontic treatment through 16S ribosomal RNA (GS) gene sequencing and PCR. Furthermore, it can be stated that the root canal instrumentation system with rotating files maintains the quality of root preparation, reducing the operative time and also the risk of a torsional fracture within the root canal.
Archives of Health Investigation, Oct 6, 2020
The coronavirus (COVID-19) is considered a global pandemic with several respiratory syndrome caus... more The coronavirus (COVID-19) is considered a global pandemic with several respiratory syndrome caused by coronavirus 2 (SARS-CoV-2). The main symptoms of COVID-19 are fever, cough, and fatigue, while other symptoms include sputum production, diarrhea and alterations on the gastrointestinal system. The COVID-19 was recently identified in the saliva of infected patients and the transmission through of droplets and aerosols generated during clinical dental care is possible among dentists, oral health assistants and/or oral health technicians and others around the social cycle of the friendship of these health professionals, such as families and colleagues. Thus, there is very necessary to improve the several types of prevention to healthcare professionals that realize the liberation of aerosol during oral assistance. It's important to mention that these precautions are important to prevent human-to-human transmission and protect the global system of health. In addition, this care is essential to decrease the spread of COVID-19 in this moment.
Frontiers in Neuroscience, Jun 27, 2019
Teneurins are type II transmembrane proteins comprised of four phylogenetically conserved homolog... more Teneurins are type II transmembrane proteins comprised of four phylogenetically conserved homologs (Ten-1-4) that are highly expressed during neurogenesis. An additional bioactive peptide named teneurin C-terminal-associated peptide (TCAP-1-4) is present at the carboxyl terminal of teneurins. The possible correlation between the Ten/TCAP system and brain injuries has not been explored yet. Thus, this study examined the expression of these proteins in the cerebral cortex after mechanical brain injury. Adult rats were subjected to cerebral cortex injury by needle-insertion lesion and sacrificed at various time points. This was followed by analysis of the lesion area by immunohistochemistry and conventional RT-PCR techniques. Control animals (no brain injury) showed only discrete Ten-2-like immunoreactive pyramidal neurons in the cerebral cortex. In contrast, Ten-2 immunoreactivity was significantly up-regulated in the reactive astrocytes in all brain-injured groups (p < 0.0001) when compared to the control group. Interestingly, reactive astrocytes also showed intense immunoreactivity to LPHN-1, an endogenous receptor for the Ten-2 splice variant named Lasso. Semi-quantitative analysis of Ten-2 and TCAP-2 expression revealed significant increases of both at 48 h, 3 days and 5 days (p < 0.0001) after brain injury compared to the remaining groups. Immortalized cerebellar astrocytes were also evaluated for Ten/TCAP expression and intracellular calcium signaling by fluorescence microscopy after TCAP-1 treatment. Immortalized astrocytes expressed additional Ten/TCAP homologs and exhibited significant increases in intracellular calcium concentrations after TCAP-1 treatment. This study is the first to demonstrate that Ten-2/TCAP-2 and LPHN-1 are upregulated in reactive astrocytes after a mechanical brain injury. Immortalized cerebellar astrocytes expressed Ten/TCAP homologs and TCAP-1 treatment stimulated intracellular calcium signaling. These findings disclose a new functional role of the Ten/TCAP system in astrocytes during tissue repair of the CNS.
Journal of Periodontology, Feb 21, 2019
This is the author manuscript accepted for publication and has undergone full peer review but has... more This is the author manuscript accepted for publication and has undergone full peer review but has not been through the copyediting, typesetting, pagination and proofreading process, which may lead to differences between this version and the Version of Record. Please cite this article as
Archives of Health Investigation, Apr 11, 2022
Interrupções nos ritmos hormonais, sono, padrões alimentares e exposição à luz ocorrem durante o ... more Interrupções nos ritmos hormonais, sono, padrões alimentares e exposição à luz ocorrem durante o trabalho em turnos. O sono é afetado pelo trabalho em turnos, em parte devido à incapacidade de mudar o ritmo circadiano do sono para corresponder ao horá rio de trabalho. Mudanças no estilo de vida que causam distúrbios no ritmo circadiano podem ter uma influência negativa no comportamento da saúde bucal, como a frequência na escovação, e ingestão de açúcar. A partir disso, nossa hipótese é de que trabalhadores noturnos podem estar mais suscetíveis a problemas de saúde bucal como perda de dentes e dentes cariados. Com isso, essa mini revisão tenta elucidar as possíveis relações entre o trabalho noturno e a saúde bucal, bem como se há uma possível correlação entra ambas. Embora haja poucos estudos relacionando o trabalho noturno e seus efeitos na saúde bucal, há fortes indícios de que o trabalho noturno afeta a saúde periodontal da população em diferentes níveis principalmente quando comparado a indivíduos saudáveis que trabalham em períodos regulares que não sejam no turno da noite, aceitando-se a hipótese levantada no estudo.
Journal of clinical images and medical case reports, Sep 12, 2022
Journal of Endodontics, Jun 1, 2017
Introduction: Our previous studies have shown that periapical lesions (PLs) in rats cause systemi... more Introduction: Our previous studies have shown that periapical lesions (PLs) in rats cause systemic disorders such as increased tumor necrosis factor-a plasma levels, insulin resistance, and impairment in insulin signal transduction in muscle tissue. However, the mechanisms involved in these alterations are not fully understood. Under chronic inflammatory conditions such as obesity, it has been shown that the skeletal muscle is affected by inflammation, and the number of resident macrophages that are associated with impairments of insulin action and sensitivity is increased. This study aimed to investigate the presence of macrophages, activation of inflammatory pathways in muscle tissue, glycemia, and insulinemia of rats with PLs. Methods: Sixty Wistar rats were distributed into a control group; a group with 1 PL (1PL), which was induced in the right maxillary first molar; and a group with 4 PLs (4PL), which were induced in the right upper and lower first and second molars. We quantified macrophage content by immunohistochemistry for the F4/80 protein. We evaluated Jun N-terminal kinase and IKKa/b phosphorylation status in the muscle tissue by Western blotting. Serum levels of lipopolysaccharide (LPS) and HSP70 and plasma levels of glucose and insulin were assessed by using commercial kits. Results: The 1PL and 4PL groups showed increase in macrophage content, IKKa/b, and Jun N-terminal kinase phosphorylation status, serum LPS and HSP70 levels, and insulin resistance and no changes in glycemia and insulinemia compared with the control group. There was no difference in these parameters between the 1PL and 4PL groups. Conclusions: PLs promoted an increase in macrophage infiltration, activation of inflammatory pathways in muscle tissue, and serum concentrations of HSP70 and LPS in rats. The present study improves the knowledge on the impact of oral inflammations on the development of systemic alteration, which can induce insulin resistance.
Archives of Health Investigation, Oct 15, 2021
The relationship between maternal health and the fetal environment has been widely discussed in r... more The relationship between maternal health and the fetal environment has been widely discussed in recent decades. Several studies have shown that insults that occur during the perinatal period modify fetal development, leading to harmful long-term results that culminate in pathologies in adulthood. Periodontal diseases (PD) comprise a wide range of inflammatory conditions that affect the supporting structures of the teeth (the gingiva, bone and periodontal ligament). Apical periodontitis (AP) is an inflammation at the apex of the thoot root usually caused by infection by bacteria from the root canal system. The present study aims to show the effects of maternal oral inflammations on the offspring health. The study provides information that both, maternal PD and AP, have negative effects in the short and long-term on the offspring health. Thus, adequate oral health can prevent adverse pregnancy outcomes and permanent changes in adult offspring. That is, oral hygiene during pregnancy should be encouraged.
Archives of Health Investigation, Aug 2, 2021
Apical periodontitis (AP) or periapical lesion (PL) is an inflammatory disease that causes pain, ... more Apical periodontitis (AP) or periapical lesion (PL) is an inflammatory disease that causes pain, loss of bone and destruction of other tissues of teeth support. This condition could be caused by caries, dental trauma and/or periapical microleakage wich is regulated by the immune system or inflammatory response. Several analyses have shown that endodontic infection can cause systemic alterations like stroke, insulin resistance, cardiovascular diseases and to contribute to the appearance of neurodegenerative disorders. The prefrontal cortex (PfC), positioned in the most anterior region of the brain, is essentially involved behavioral abilities, intellectual roles, cognition, learning and other functions. Neurons with laminar distribution in the PfC, especially in the lower layers express several types of calcium-binding proteins (CaBPs) like parvalbumin, calbindin and calretinin that are involved in calcium buffering into the brain (CNS). These proteins are co-localized with GABAergic neurons, main inhibitory cells into the brain. Piece of evidence has pointed out a relationship between apical periodontitis and neurodegenerative diseases where microorganisms and/or toxins produced in the teeth infection can penetrate the bloodstream, infiltrate the blood-brain barrier limit and active astrocytes and microglial cells in the central nervous system (CNS) and increase the releasing of pro-inflammatory cytokines promoting pyramidal and non-pyramidal neuronal degeneration. Thus, is there a relationship among apical periodontitis, oral hygiene and prefrontal cortex and neurodegenerat ive disorders?
Frontiers of oral and maxillofacial medicine, 2021
The severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) causes fever, dry cough, pain, h... more The severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) causes fever, dry cough, pain, headache, tiredness, anorexia, and loss of smell and/or taste. Patients infected with COVID-19 may also exhibit neurological alterations, such as delirium, ischemic and hemorrhagic strokes, and epileptic seizures. In accordance with the World Health Organization (WHO), in March 2020, COVID-19 became a new pandemic, affecting the lives of people, the health care system, the economy, and other aspects of life around the world (1). Epilepsy affects about 50 million people worldwide, and the whole mechanism of action of this pathology still unclear; however, it has been suggested that the increase of neuronal excitability in several areas of the brain can spread the seizures caused by the imbalance of many neurotransmitters (2). Currently, there has been an increase in the evidence indicating that astrocytes have a role in epilepsy, according to which this type of cell modulates neuronal excitation and inhibition in the brain, composing the tripartite synapse (3). Several studies have revealed the presence of molecules responsible for the COVID-19 infection in oral tissues and cells, such as tongues, salivary glands, mucosa, junctional epithelium, oral gingival epithelium, and connective tissue (4). Furthermore, recent studies have shown a relationship between SARS-CoV-2 infections and other systemic disorders, such as hypertension, diabetes mellitus, obesity, and periodontitis (5,6). In addition, metagenomic analyses have shown a connection between SARS-CoV-2 and cariogenic or periodontopathic bacteria (7).
Revista de Cirurgia e Traumatologia Buco-maxilo-facial, Mar 1, 2016
MedNEXT Journal of Medical and Health Sciences, May 25, 2022
International Endodontic Journal
AimTo analyse the effects of melatonin (ME) treatment on oxidative stress and insulin resistance ... more AimTo analyse the effects of melatonin (ME) treatment on oxidative stress and insulin resistance (IR) in rats with apical periodontitis (AP) fed a high‐fat diet (HFD).MethodologyEighty 60‐day‐old rats were divided into eight groups: control (CN), AP, HFD with AP (HFDAP), control with ME (CNME), AP with ME (APME), HFD with ME (HFDME) and HFD with AP+ME (HFDAPME). The animals from the HFD groups were fed a HFD throughout the experimental period. On day 7, the animals from the AP groups were subjected to experimental AP, and after 70 days, the ME groups were treated for 30 days. Glycaemia, insulinaemia, homeostatic model assessment for IR index, tumour necrosis factor‐α (TNF‐α), and interleukin‐6 were analysed in plasma using biochemical tests and enzyme‐linked immunosorbent assay. Thiobarbituric acid‐reactive substances (TBARS), carbonyl protein (CP), superoxide dismutase (SOD), catalase, glutathione peroxidase, glutathione (GSH) and total antioxidant capacity (ferric reducing antioxi...
ARCHIVES OF HEALTH INVESTIGATION
Interrupções nos ritmos hormonais, sono, padrões alimentares e exposição à luz ocorrem durante o ... more Interrupções nos ritmos hormonais, sono, padrões alimentares e exposição à luz ocorrem durante o trabalho em turnos. O sono é afetado pelo trabalho em turnos, em parte devido à incapacidade de mudar o ritmo circadiano do sono para corresponder ao horário de trabalho. Mudanças no estilo de vida que causam distúrbios no ritmo circadiano podem ter uma influência negativa no comportamento da saúde bucal, como a frequência na escovação, e ingestão de açúcar. A partir disso, nossa hipótese é de que trabalhadores noturnos podem estar mais suscetíveis a problemas de saúde bucal como perda de dentes e dentes cariados. Com isso, essa mini revisão tenta elucidar as possíveis relações entre o trabalho noturno e a saúde bucal, bem como se há uma possível correlação entra ambas. Embora haja poucos estudos relacionando o trabalho noturno e seus efeitos na saúde bucal, há fortes indícios de que o trabalho noturno afeta a saúde periodontal da população em diferentes níveis principalmente quando comp...
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Papers by Gestter Willian Lattari Tessarin