Papers by Gabriela Scortegagna
The polymerase chain reaction (PCR) and hemaglutination (HA) assay were used to detect canine par... more The polymerase chain reaction (PCR) and hemaglutination (HA) assay were used to detect canine parvovirus (CPV) in feces from young dogs with gastroenteritis. The hemaglutination inhibition (HI) assay was used to detect the prevalence of anti-CPV antibodies in a non-vaccinated dog population. In addition, hiperimmune serum was used to investigate the phenotypic variability of a vaccine strain and two field isolates of CPV. Thirty fecal samples obtained from dogs with diarrhea were submitted to PCR and HA, and 185 serum samples were submitted to HI to detect anti-CPV antibodies. Nine (30%) of the samples demonstrated HA on fecal dilutions equal to or above 1:64 and were considered positive by this test; nine (30%) fecal samples had HA activity on dilution from 1:2 to 1:32 and were considered negative, and the remaining samples were negative. All samples with HA activity at dilutions above 1:64 were also positive to PCR and, out of the nine samples with HA activity at dilutions between...
Anestesicos locais, contendo ou nao vasoconstritores, sao utilizados para realizacao da maioria d... more Anestesicos locais, contendo ou nao vasoconstritores, sao utilizados para realizacao da maioria dos procedimentos odontologicos. Porem, seu uso inadequado, principalmente em casos de alteracao sistemica, pode acarretar serios riscos para a saude do paciente. O objetivo deste estudo foi avaliar o nivel de conhecimento dos cirurgioes dentistas (CDs) com relacao a utilizacao de anestesicos locais, frente a pacientes especiais com diabetes, hipertensao, cardiopatias, gestantes e com hipertireoidismo. O estudo teve um delineamento quantitativo do tipo transversal com dados coletados atraves de questionario adaptado pela equipe como unico instrumento para coleta de dados. Os dados foram submetidos a analise estatistica descritiva e inferencial. De 200 participantes, 17% eram academicos formandos em odontologia, e, dentre os formados, a maioria (34 %) tinha de 1 a 5 anos ou de 6 a 10 anos (16%) de formacao. Do total de participantes graduados, 43% nao possuiam especializacao e 57% eram esp...
Clinical Immunology, 2016
High dose immunosuppression followed by autologous hematopoietic stem cell transplantation (AHSCT... more High dose immunosuppression followed by autologous hematopoietic stem cell transplantation (AHSCT) induces prolonged clinical remission in multiple sclerosis (MS) patients. However, how patient immune profiles are associated with clinical outcomes has not yet been completely elucidated. In this study, 37 MS patients were assessed for neurological outcomes, thymic function and long-term immune reconstitution after AHSCT. Patients were followed for a mean (SD) of 68.5 (13.9) months post-transplantation and were retrospectively clustered into progression-and non-progression groups, based on Expanded Disease Status Scale (EDSS) outcomes at last visit. After AHSCT, both patient groups presented increased regulatory T-cell subset counts, early expansion of central-and effector-memory CD8 + T-cells and late thymic reactivation. However, the non-progression group presented early expansion of PD-1 + CD8 + T-cells and of PD-1-expressing CD19 + B-cells. Here, we suggest that along with increased numbers of regulatory T-cell subsets, PD-1 inhibitory signaling is one possible immunoregulatory mechanism by which AHSCT restores immune tolerance in MS patients.
Journal of Oral Investigations
Anestésicos locais, contendo ou não vasoconstritores, são utilizados para realização da maioria d... more Anestésicos locais, contendo ou não vasoconstritores, são utilizados para realização da maioria dos procedimentos odontológicos. Porém, seu uso inadequado, principalmente em casos de alteração sistêmica, pode acarretar sérios riscos para a saúde do paciente. O objetivo deste estudo foi avaliar o nível de conhecimento dos cirurgiões dentistas (CDs) com relação à utilização de anestésicos locais, frente à pacientes especiais com diabetes, hipertensão, cardiopatias, gestantes e com hipertireoidismo. O estudo teve um delineamento quantitativo do tipo transversal com dados coletados através de questionário adaptado pela equipe como único instrumento para coleta de dados. Os dados foram submetidos à análise estatística descritiva e inferencial. De 200 participantes, 17% eram acadêmicos formandos em odontologia, e, dentre os formados, a maioria (34 %) tinha de 1 a 5 anos ou de 6 a 10 anos (16%) de formação. Do total de participantes graduados, 43% não possuíam especialização e 57% eram esp...
Full Dentistry in Science
Frontiers in immunology, 2017
Autologous hematopoietic stem cell transplantation (AHSCT) increases C-peptide levels and induces... more Autologous hematopoietic stem cell transplantation (AHSCT) increases C-peptide levels and induces insulin independence in patients with type 1 diabetes. This study aimed to investigate how clinical outcomes may associate with the immunological status, especially concerning the balance between immunoregulation and autoreactivity. Twenty-one type 1 diabetes patients were monitored after AHSCT and assessed every 6 months for duration of insulin independence, C-peptide levels, frequencies of islet-specific autoreactive CD8(+) T cells (CTL), regulatory lymphocyte subsets, thymic function, and T-cell repertoire diversity. In median follow-up of 78 (range 15-106) months, all patients became insulin-independent, resuming insulin after median of 43 (range 6-100) months. Patients were retrospectively divided into short- or prolonged-remission groups, according to duration of insulin independence. For the entire follow-up, CD3(+)CD4(+) T-cell numbers remained lower than baseline in both groups...
Scientia Medica, May 4, 2009
Objetivos: analisar a prevalência de artralgia no pré e no pós-operatório tardio de cirurgia bari... more Objetivos: analisar a prevalência de artralgia no pré e no pós-operatório tardio de cirurgia bariátrica, comparar o índice de massa corporal (IMC) dos indivíduos obesos nos períodos pré e pós-operatório, verificar a associação entre teste de caminhada de seis minutos e artralgia e avaliar a correlação entre teste de caminhada de seis minutos e IMC.
Ciencia Rural, 2008
The polymerase chain reaction (PCR) and hemaglutination (HA) assay were used to detect canine par... more The polymerase chain reaction (PCR) and hemaglutination (HA) assay were used to detect canine parvovirus (CPV) in feces from young dogs with gastroenteritis.
Sci Med, Jun 1, 2009
Objetivos: analisar a prevalência de artralgia no pré e no pós-operatório tardio de cirurgia bari... more Objetivos: analisar a prevalência de artralgia no pré e no pós-operatório tardio de cirurgia bariátrica, comparar o índice de massa corporal (IMC) dos indivíduos obesos nos períodos pré e pós-operatório, verificar a associação entre teste de caminhada de seis minutos e artralgia e avaliar a correlação entre teste de caminhada de seis minutos e IMC.
Objetivou-se investigar a freqüência de aglutininas anti-Brucella abortus em caprinos e ovinos do... more Objetivou-se investigar a freqüência de aglutininas anti-Brucella abortus em caprinos e ovinos do Sertão do Estado de Pernambuco, Brasil. Foram processadas 700 amostras de soros sangüíneos, das quais 340 eram da espécie caprina (115 machos e 225 fêmeas) e 360 (136 machos e 224 fêmeas) ovina. Empregou-se a técnica do antígeno acidificado tamponado (AAT) corado com rosa bengala (RB). Das 340 amostras de caprinos avaliadas, duas (0,6%) foram reagentes ao AAT. Não se observaram associações significativas para as variáveis faixa etária (p= 0,430), raça (p= 0,936) e sexo (p= 0,562). Das 360 amostras de ovinos, nove (2,5%) foram reagentes. Também não houve associação significativa entre as variáveis analisadas e a soropositividade para brucelose: faixa etária (p= 0,522); raça (p= 0,576) e sexo (p= 0,461). Verificou-se associação significativa (p= 0,042) entre as espécies estudadas e soropositividade para brucelose nos animais investigados. A soropositividade para Brucella abortus em caprin...
The polymerase chain reaction (PCR) and hemaglutination (HA) assay were used to detect canine par... more The polymerase chain reaction (PCR) and hemaglutination (HA) assay were used to detect canine parvovirus (CPV) in feces from young dogs with gastroenteritis.
Ciência Animal …, 2008
Diversas doenças infectocontagiosas são importantes causas de prejuízos na produção leiteira. No ... more Diversas doenças infectocontagiosas são importantes causas de prejuízos na produção leiteira. No entanto, poucos estudos são realizados com o objetivo de determinar a real prevalência e incidência dos agentes infecciosos nos rebanhos leiteiros no Brasil. Para determinar a prevalência das infecções pelo herpesvírus bovino (BoHV), vírus da diarréia viral bovina (BVDV), vírus da leucose enzoótica bovina (BLV) e Neospora caninun (NC), amostras de sangue foram coletadas de duzentos bovinos, pertencentes a 26 propriedades leiteiras, de quatro municípios da região Nordeste do Estado do Rio Grande do Sul, e testadas para a presença de anticorpos específicos. Estimou-se o número de amostras coletadas em cada propriedade considerando-se uma prevalência mínima de 30% para cada infecção, e com um intervalo de confiança de 95%. Detectaram-se anticorpos contra o BoHV e BVDV em bovinos de quinze (57,7%) propriedades e bovinos positivos para o BLV e NC em dezesseis (61,5%) e 24 (92,3%) propriedades, respectivamente. Todos os animais do estudo foram negativos para brucelose e tuberculose. Os dados obtidos indicam que se trata de microorganismos amplamente difundidos nas populações de bovinos de leite e poderiam contribuir para a redução na produção e redução dos índices reprodutivos.
Clinical immunology (Orlando, Fla.), Jan 15, 2016
High dose immunosuppression followed by autologous hematopoietic stem cell transplantation (AHSCT... more High dose immunosuppression followed by autologous hematopoietic stem cell transplantation (AHSCT) induces prolonged clinical remission in multiple sclerosis (MS) patients. However, how patient immune profiles are associated with clinical outcomes has not yet been completely elucidated. In this study, 37 MS patients were assessed for neurological outcomes, thymic function and long-term immune reconstitution after AHSCT. Patients were followed for a mean (SD) of 68.5 (13.9) months post-transplantation and were retrospectively clustered into progression- and non-progression groups, based on Expanded Disease Status Scale (EDSS) outcomes at last visit. After AHSCT, both patient groups presented increased regulatory T-cell subset counts, early expansion of central- and effector-memory CD8(+)T-cells and late thymic reactivation. However, the non-progression group presented early expansion of PD-1-expressing CD8(+)T-cells. Here, we suggest that along with increased numbers of regulatory T-...
PLOS ONE, 2016
Organ dysfunction is a major concern in sepsis pathophysiology and contributes to its high mortal... more Organ dysfunction is a major concern in sepsis pathophysiology and contributes to its high mortality rate. Neutrophil extracellular traps (NETs) have been implicated in endothelial damage and take part in the pathogenesis of organ dysfunction in several conditions. NETs also have an important role in counteracting invading microorganisms during infection. The aim of this study was to evaluate systemic NETs formation, their participation in host bacterial clearance and their contribution to organ dysfunction in sepsis. C57Bl/6 mice were subjected to endotoxic shock or a polymicrobial sepsis model induced by cecal ligation and puncture (CLP). The involvement of cf-DNA/NETs in the physiopathology of sepsis was evaluated through NETs degradation by rhDNase. This treatment was also associated with a broad-spectrum antibiotic treatment (ertapenem) in mice after CLP. CLP or endotoxin administration induced a significant increase in the serum concentrations of NETs. The increase in CLP-induced NETs was sustained over a period of 3 to 24 h after surgery in mice and was not inhibited by the antibiotic treatment. Systemic rhDNase treatment reduced serum NETs and increased the bacterial load in non-antibiotic-treated septic mice. rhDNase plus antibiotics attenuated sepsis-induced organ damage and improved the survival rate. The correlation between the presence of NETs in peripheral blood and organ dysfunction was evaluated in 31 septic patients. Higher cf-DNA concentrations were detected in septic patients in comparison with healthy controls, and levels were correlated with sepsis severity and organ dysfunction. In conclusion, cf-DNA/NETs are formed during sepsis and are associated with sepsis severity. In the experimental setting, the degradation of NETs by rhDNase attenuates organ damage only when combined with antibiotics, confirming that NETs take part in sepsis pathogenesis. Altogether, our results suggest that NETs are important for host bacterial control and are relevant actors in the pathogenesis of sepsis.
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Papers by Gabriela Scortegagna