Papers by LUZ MARINA GOMEZ ARIZA
Gene Therapy, 2011
Efficient transduction of the peripheral nervous system (PNS) is required for gene therapy of acq... more Efficient transduction of the peripheral nervous system (PNS) is required for gene therapy of acquired and inherited neuropathies, neuromuscular diseases and for pain treatment. We have characterized the tropism and transduction efficiency of different adeno-associated vectors (AAV) pseudotypes after sciatic nerve injection in the mouse. Among the pseudotypes tested, AAV2/1 transduced both Schwann cells and neurons, AAV2/2 infected only sensory neurons and AAV2/8 preferentially transduced Schwann cells. AAV2/8 expression in the sciatic nerve was detected up to 10 weeks after administration, the latest time point analyzed. The injected mice developed neutralizing antibodies against all AAVs tested; the titers were higher against AAV2/1 than AAV2/2 and were the lowest for AAV2/8, correlating with a higher transgene expression overtime. AAV2/8 coding for ciliary neurotrophic factor (CNTF) led to an upregulation of P0 and PMP22 myelin proteins, four weeks after transduction of injured sciatic nerves. Importantly, CNTF-transduced mice showed a significant increase in both GAP43 expression in sensory neurons, a marker of axonal regeneration, and the compound muscle action potential. These results prove the utility of AAV8 as a gene therapy vector for Schwann cells to treat myelin disorders or to improve nerve regeneration.
International Journal of Human Sciences Research, Dec 20, 2022
All content in this magazine is licensed under a Creative Commons Attribution License. Attributio... more All content in this magazine is licensed under a Creative Commons Attribution License. Attribution-Non-Commercial-Non-Derivatives 4.0 International (CC BY-NC-ND 4.0).
From a critical analysis it was studied the relationship between the PEMP and the tourism sustain... more From a critical analysis it was studied the relationship between the PEMP and the tourism sustainability of Cartagena de Indias, being this document a tool of administration and management required to ensure development, joining efforts with a global vision for Latin America and the Caribbean, a study carried out through the Participation in different events organized from governmental estates, forming working tables, constructive dialogues and focus groups, formed by interdisciplinary groups, from all strata and professions, giving a holistic vision to the construction and validation of information.The research evidenced the high degree of interference that the PEM has in the Historic Center of Cartagena de India’s certification, given its high degree of effectiveness as an development generator instrument, synchronizing the lines that include both processes to motivate their interest and commitment to a responsible and rallied job from the different interest groups, for the conser...
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Papers by LUZ MARINA GOMEZ ARIZA