RESUMO: Doença do corpúsculo de inclusão (IBD) é uma enfermidade caracterizada por corpúsculos in... more RESUMO: Doença do corpúsculo de inclusão (IBD) é uma enfermidade caracterizada por corpúsculos intracitoplasmáticos em diversos tecidos, principalmente no sistema nervoso central, responsável pelos principais sinais clínicos atribuídos à doença que acomete Boas e Phytons de cativeiro; essa enfermidade tem sido uma preocupação mundial devido à alta morbidade e mortalidade. O diagnóstico é feito pela visualização dos corpúsculos causados por um Arenavírus modificado. Salmonella sp. pertence à microflora de animais de sangue frio e quente, e é um patógeno oportunista que pode causar quadros gastrointestinais ou septicêmicos. Em répteis a Salmonella sp. é a bactéria com maior frequência de citações em espondilites e osteomielites. Relata-se um caso de uma jiboia (Boa constrictor constrictor) que apresentava restrição de movimento e múltiplos granulomas dorsais nas vértebras; à radiografia evidenciaram-se regiões fraturadas. Após meses de tratamentos sem melhora clínica e o aparecimento ...
Incorrect management techniques can contribute negatively to the health of captive birds. Among t... more Incorrect management techniques can contribute negatively to the health of captive birds. Among these various diseases that may develop, the endoparasites is one of the most commonly reported. Was studied the occurrence of endoparasites in 11 parrots Amazona rhodocorytha in captivity of Commercial Breeding and Conservationist. Collected a pool of fecal samples for subsequent laboratory examination by the methods Willis Mollay, Faust and Hoffman modify. The diagnosis was positive for Eimeria sp. The animals were treated for five days with trimethoprim sulfamethoxazole associated with, 50mg/ml suspension at a dose of 30mg/kg every 24 hours, along with changes in the management sanitary. In late of treatment, there was another coproparasitologic to certify the absence of parasites. The result was negative for endoparasites.
Cadmium (Cd) is a highly toxic contaminant causing diverse physiologic damage through the food ch... more Cadmium (Cd) is a highly toxic contaminant causing diverse physiologic damage through the food chain, possibly influencing the demography of wild populations. Thus study aimed to evaluate the feasibility of using Bothrops jararaca as an indicator of Cd contamination. For this purpose, 20 B. jararaca individuals were divided into four groups: a control group and three treatment groups (n = 5 in each group), which received Cd-contaminated food (900 mg kg −1) once, twice, or thrice, with a 15-day interval between meals. Animal fitness was evaluated by righting response behavior. In addition, genotoxic effects (comet assay damage index-DI), Cd accumulation in the kidney, liver, heart, gallbladder, fat, venom gland, blood, and tail, and Cd contents in the feces were evaluated. Righting response was negatively related to the number of received meals. Comet assay DI was significantly increased in animals that were fed two or three times. After three contaminated meals, Cd distribution within the body was as follows (µg/gmedian): liver (6.24) > kidney (1.88) > venom gland (0.93) > fat (0.85) > gallbladder (0.65) > tail (0.46) > blood (0.45) > heart (0.25). Cd contents in the liver, kidney, and feces were significantly greater in animals that consumed Cd-contaminated food for two or three times. However, Cd accumulation in the liver started to appear after the first meal comprising the highest Cd concentration, while accumulation in the kidney appeared after the third feeding. It can be concluded that B. jararaca is a feasible model for biomonitoring Cd using the liver as a prognostic organ for the bioaccumulation as well as righting response and the damage index as indicators of Cd effects at an experimental level. Field monitoring feces stood out as the most suitable indicator for Cd ingestion by B. jararaca.
This study aimed to report the first occurrence of Leporacarus gibbus in a domestic rabbit (Oryct... more This study aimed to report the first occurrence of Leporacarus gibbus in a domestic rabbit (Oryctolagus cuniculus) from the state of Espírito Santo, Brazil. The two-year-old male rabbit came from a rural area and was admitted to the Veterinary Hospital Professor Ricardo Alexandre Hippler with intense pruritus throughout the body, severe hair loss, crustal lesions on the outer surface of the ears, and desquamation mainly in the dorsal region. A skin scraping and trichogram were performed. In the microscopic evaluation of the hairs obtained from the dorsal region, mites with morphology compatible with L. gibbus were observed. Cheyletiella parasitovorax and Psoropotes cuniculi were also detected in the same region. Treatment with ivermectin (0.4 mg/kg) was prescribed with three applications every 14 days, over a period of six weeks. This report presents the first description of the occurrence of L. gibbus in the state of Espírito Santo concomitant with poly-infestation of C. parasitovorax and P. cuniculi, and it is hoped that this will provide a research tool for future work in the region.
Revista de Patologia Tropical / Journal of Tropical Pathology, 2015
Platynosomum fastosum é um parasito de felinos, entre os quais os gatos domésticos, sobretudo os ... more Platynosomum fastosum é um parasito de felinos, entre os quais os gatos domésticos, sobretudo os originados de locais onde o clima é tropical e subtropical. Este é o parasito hepático mais comum de gatos domésticos e se localiza geralmente nas vias biliares e vesícula biliar, mas também pode ser encontrado no intestino delgado e pâncreas. A infecção se dá por meio da ingestão de lagartixa contendo as metacercárias que, nas vias biliares de gatos domésticos, causam uma colangite. O objetivo do presente trabalho foi relatar a ocorrência de P. fastosum, verificada por meio de necropsia de um gato doméstico, assintomático, com histórico de morte súbita, recebido no setor de Patologia Animal do Hospital Veterinário Ricardo Alexandre Hippler, Espírito Santo, Brasil. No exame macroscópico, foram observados exemplares adultos de P. fastosum no interior dos ductos biliares que se encontravam visivelmente dilatados. No exame histopatológico, foram confirmadas dilatação, presença de parasitos ...
Dogs produce fewer sperm than other species. Furthermore, for French Bulldogs, anatomical peculia... more Dogs produce fewer sperm than other species. Furthermore, for French Bulldogs, anatomical peculiarities, low libido, and increased sensitivity to stress could cause further reductions in sperm count. The objective was to compare effects of semen collection at 24- versus 48-h intervals on semen quality of French Bulldogs. Five purebred French Bulldogs, 19 to 48 months old, were subjected to 5 semen collections (24 h apart). After a 30-day rest, collections were repeated, but the interval between collections was 48 h. Semen was collected (all 3 fractions) by digital manipulation without female stimuli. Volume was measured in a 20-mL syringe. Sperm concentration was determined with a Neubauer counting chamber. Motility and vigor were evaluated with a coverslipped drop of semen on a slide (preheated to 37°C). Motility was expressed as a percentage of motile sperm, and vigor was classified on a scale of 1 to 5. Morphology was evaluated by the panoptic method; 100 cells were counted and r...
The intrinsic yield of spermatogenesis and the supporting indexes of the Sertoli cells are the be... more The intrinsic yield of spermatogenesis and the supporting indexes of the Sertoli cells are the best indicators for the spermatic production capacity in a species. The aim of the present study was to quantify the intrinsic yield of the spermatogenetic process, as well as the Sertoli cell index and spermatic reserves. Testicular fragments of five adult African lions was fixed in 4% glutaric aldehyde, dehydrated at increasing alcohol concentrations, included into hydroxyethyl methacrylate, and were cut into 4 microm thickness. In the seminiferous epithelium of the African lions, 10.3 primary spermatocytes at pre-leptotene phase are produced by the type-A spermatogonia. During meiotic divisions, only 2.7 spermatids were produced from the primary spermatocytes. The general spermatogenesis production in the African lions was approximately 22.1 cells, and each Sertoli cell was able to sustain and maintain approximately 14.9 cells of the germinative line, from which 7.9 are round spermatids. A total of 103x10(6) spermatozoa are produced by each testis gram at each cycle of the seminiferous epithelium. The spermatic reserve of lion is below the amplitude observed in mammals.
Puma or sussuarana (Puma concolor) is the second largest feline in the American continent and has... more Puma or sussuarana (Puma concolor) is the second largest feline in the American continent and has an ample latitudinal distribution in very diverse habitats. In relation to its conservation status, the puma is considered an extinction-threatened species. The study of the testis morphology and the spermatogenic process in a species is fundamental for establishing the physiologic patterns that will make possible the selection of the protocols for assisted reproduction. A number of peculiarities associated with the reproductive biology of specific species such as the duration of spermatogenic process can be used to determine the frequency of sperm collection. Nine adult male pumas maintained in captivity were used to determine the relative frequency of stages in the seminiferous epithelium cycle. Three of them received intra-testicular injections of 0.1 ml tritiated thymidine to determine the duration of the seminiferous epithelium cycle, and were subjected to biopsy 7 days later. The cycle of the seminiferous epithelium in puma was didactically described into eight stages by the tubular morphology method. The total duration of one seminiferous epithelium cycle in puma was calculated to be 9.89 days, and approximately 44.5 days are required for development of spermatozoon from spermatogonia. The duration of spermiogenesis, prophase and other events of meiosis were 14.08,
Six adult Leopardus tigrinus (oncilla) were studied to characterize stages of the seminiferous ep... more Six adult Leopardus tigrinus (oncilla) were studied to characterize stages of the seminiferous epithelium cycle and its relative frequency and duration, as well as morphometric parameters of the testes. Testicular fragments were obtained (incisional biopsy), embedded (glycol methacrylate), and histologic sections examined with light microscopy. The cycle of the seminiferous epithelium was categorized into eight stages (based on the tubular morphology method). The duration of one seminiferous epithelium cycle was 9.19 d, and approximately 41.37 d were required for development of sperm from spermatogonia. On average, diameter of the seminiferous tubules was 228.29 m, epithelium height was 78.86 m, and there were 16.99 m of testicular tubules per gram of testis. Body weight averaged 2.589 kg, of which 0.06 and 0.04% were attributed to the testis and seminiferous tubules, respectively. In conclusion, there were eight distinct stages in the seminiferous epithelium, the length of the seminiferous epithelium cycle was close to that in domestic cats and cougars, and testicular and somatic indexes were similar to those of other carnivores of similar size.
Causes of dental infections can be related to failed dental eruption, malocclusion, abrasion, fra... more Causes of dental infections can be related to failed dental eruption, malocclusion, abrasion, fractures with or without exposure of the dental pulp, and periodontal disease. Reports of oral myiasis in megavertebrates in captivity are infrequent, perhaps due to the difficulty in observing the oral cavity in such species. This report describes a case of oral myiasis in an adult male hippopotamus in the gingival area and alveolar mucosa of the left mandibular canine tooth.
Abstract: Quantitative computed tomography (QCT) is a highly sensitive, applicable technique for... more Abstract: Quantitative computed tomography (QCT) is a highly sensitive, applicable technique for determining the x-ray attenuation of organs. This technique reveals great precision in the detection of alterations in the x-ray attenuation of hepatic parenchyma, although the lack of studies establishing normal values limits its application in wild animals. The objective of this study was to establish mean hepatic attenuation values in four healthy juvenile sea turtles (Chelonia mydas) using QCT. Helical computed tomography scans were performed and regions of interest selected in the liver after multi-planar reconstruction images were obtained. The mean attenuation value for the hepatic parenchyma in these four turtles was 60.09 ± 5.3 standard deviation Hounsfield units. Determining normal x-ray attenuation values of the liver increases knowledge of the computed tomographic anatomy of this species and may be useful in the investigation of hepatic diseases.
Pathological and parasitological characterization of Prosthenorchis elegans in a free-ranging mar... more Pathological and parasitological characterization of Prosthenorchis elegans in a free-ranging marmoset Callithrix geofroyi from the Brazilian Atlantic Forest.
The endocrine portion of mammal testicle is represented by Leydig cells which, together with conn... more The endocrine portion of mammal testicle is represented by Leydig cells which, together with connective cells, leukocytes, blood and lymphatic vessels, form the intertubular space. The arrangement and proportion of these components vary in the different species of mammals and form mechanisms that keep the testosterone level--the main product of the Leydig cell--two to three times higher in the interstitial fluid than in the testicular blood vessels and 40-250 times higher in these than in the peripheral blood. Marked differences are observed among animal species regarding the abundance of Leydig cells, loose connective tissue, development degree and location of the lymphatic vessels and their topographical relationship with seminiferous tubules. In the jaguar about 13% of the testicular parenchyma is occupied by Leydig cells, 8.3% by connective tissue and 0.3% by lymphatic vessels. Although included in standard II, as described in the literature, concerning the arrangement of the intertubular space, the jaguar has grouped lymphatic vessels in the intertubular space instead of isolated ones. In the jaguar the average volume of the Leydig cell was 2386 microm3 and its average nuclear diameter was 7.7 microm. A great quantity of 2.3 microm diameter lipidic drops was observed in the Leydig cell cytoplasm of the jaguar. The Leydig cells in the jaguar occupy an average 0.0036% of the body weight and the average number per gram of testicle was within the range for most mammals: between 20 and 40 million.
O objetivo deste trabalho foi desenvolver um protocolo de extração hormonal à partir de fezes de ... more O objetivo deste trabalho foi desenvolver um protocolo de extração hormonal à partir de fezes de onça-pintada (Panthera onca) e validar uma metodologia de análise destes extratos por eletroforese capilar, verificando a eficiência analítica desta técnica no estudo dos metabólitos de esteróides sexuais em fezes, com o intuito de utilização futura desta metodologia na rotina dos laboratórios de endocrinologia. Foram testados sete tratamentos para as amostras de fezes liofilizadas: quatro protocolos de extração em diferentes solventes, um de hidrólise ácida e dois protocolos de extração em fase sólida (SPE). O protocolo de extração em acetonitrila foi satisfatório como pré-tratamento da amostra e aliado à SPE C-18 apresentou bons resultados no que diz respeito à sensibilidade, precisão, recuperação e tempo de análise. Com base nos resultados obtidos, a eletroforese capilar pode ser considerada uma ótima alternativa para a prospecção de metabólitos de hormônios, podendo ser utilizada em ...
Six adult Leopardus tigrinus (oncilla) were studied to characterize stages of the seminiferous ep... more Six adult Leopardus tigrinus (oncilla) were studied to characterize stages of the seminiferous epithelium cycle and its relative frequency and duration, as well as morphometric parameters of the testes. Testicular fragments were obtained (incisional biopsy), embedded (glycol methacrylate), and histologic sections examined with light microscopy. The cycle of the seminiferous epithelium was categorized into eight stages (based on the tubular morphology method). The duration of one seminiferous epithelium cycle was 9.19 d, and approximately 41.37 d were required for development of sperm from spermatogonia. On average, diameter of the seminiferous tubules was 228.29 m, epithelium height was 78.86 m, and there were 16.99 m of testicular tubules per gram of testis. Body weight averaged 2.589 kg, of which 0.06 and 0.04% were attributed to the testis and seminiferous tubules, respectively. In conclusion, there were eight distinct stages in the seminiferous epithelium, the length of the seminiferous epithelium cycle was close to that in domestic cats and cougars, and testicular and somatic indexes were similar to those of other carnivores of similar size.
Densitometria da vértebra dorsal, osso pleural e osso neural em tartarugas verdes hígidas por tom... more Densitometria da vértebra dorsal, osso pleural e osso neural em tartarugas verdes hígidas por tomografia computadorizada quantitativa Densitometry of dorsal vertebrae, pleural bone and neural bone in healthy green sea turtles by quantitative computed tomography Recebido para publicação 01.09.11 Aprovado em 04.03.12 Devolvido pelo autor 07.06.012 CR-5943 Palavras-chave: quelônios, coluna, densidade mineral óssea, tomografia computadorizada quantitativa, Chelonia mydas. ABSTRACT Quantitative computed tomography (QCT) is a technique with high sensitivity, applicability and accuracy in the diagnosis of bone loss; however the lack of normal values for wild animals limits their clinical application. This study aims to establish medium values radiodensity trabecular bone of the dorsal vertebrae, bone pleural and neural bone in healthy juvenile sea turtles using the QTC. For this we used an experimental group of five animals that underwent helical computed tomography, and measurements of bone radiodensity conducted at the level of the third, fourth and fifth dorsal vertebrae. The medium values of radiodensity trabecular bone of the dorsal vertebrae, pleural bone, and neural bone were respectively 287.4±39.1, 1093.74±100.4, and 1089.6±86.5 Hounsfield units (HU). Further studies should be performed in different species and ages of turtles to encourage the clinical application of this technique.
RESUMO: Doença do corpúsculo de inclusão (IBD) é uma enfermidade caracterizada por corpúsculos in... more RESUMO: Doença do corpúsculo de inclusão (IBD) é uma enfermidade caracterizada por corpúsculos intracitoplasmáticos em diversos tecidos, principalmente no sistema nervoso central, responsável pelos principais sinais clínicos atribuídos à doença que acomete Boas e Phytons de cativeiro; essa enfermidade tem sido uma preocupação mundial devido à alta morbidade e mortalidade. O diagnóstico é feito pela visualização dos corpúsculos causados por um Arenavírus modificado. Salmonella sp. pertence à microflora de animais de sangue frio e quente, e é um patógeno oportunista que pode causar quadros gastrointestinais ou septicêmicos. Em répteis a Salmonella sp. é a bactéria com maior frequência de citações em espondilites e osteomielites. Relata-se um caso de uma jiboia (Boa constrictor constrictor) que apresentava restrição de movimento e múltiplos granulomas dorsais nas vértebras; à radiografia evidenciaram-se regiões fraturadas. Após meses de tratamentos sem melhora clínica e o aparecimento ...
Incorrect management techniques can contribute negatively to the health of captive birds. Among t... more Incorrect management techniques can contribute negatively to the health of captive birds. Among these various diseases that may develop, the endoparasites is one of the most commonly reported. Was studied the occurrence of endoparasites in 11 parrots Amazona rhodocorytha in captivity of Commercial Breeding and Conservationist. Collected a pool of fecal samples for subsequent laboratory examination by the methods Willis Mollay, Faust and Hoffman modify. The diagnosis was positive for Eimeria sp. The animals were treated for five days with trimethoprim sulfamethoxazole associated with, 50mg/ml suspension at a dose of 30mg/kg every 24 hours, along with changes in the management sanitary. In late of treatment, there was another coproparasitologic to certify the absence of parasites. The result was negative for endoparasites.
Cadmium (Cd) is a highly toxic contaminant causing diverse physiologic damage through the food ch... more Cadmium (Cd) is a highly toxic contaminant causing diverse physiologic damage through the food chain, possibly influencing the demography of wild populations. Thus study aimed to evaluate the feasibility of using Bothrops jararaca as an indicator of Cd contamination. For this purpose, 20 B. jararaca individuals were divided into four groups: a control group and three treatment groups (n = 5 in each group), which received Cd-contaminated food (900 mg kg −1) once, twice, or thrice, with a 15-day interval between meals. Animal fitness was evaluated by righting response behavior. In addition, genotoxic effects (comet assay damage index-DI), Cd accumulation in the kidney, liver, heart, gallbladder, fat, venom gland, blood, and tail, and Cd contents in the feces were evaluated. Righting response was negatively related to the number of received meals. Comet assay DI was significantly increased in animals that were fed two or three times. After three contaminated meals, Cd distribution within the body was as follows (µg/gmedian): liver (6.24) > kidney (1.88) > venom gland (0.93) > fat (0.85) > gallbladder (0.65) > tail (0.46) > blood (0.45) > heart (0.25). Cd contents in the liver, kidney, and feces were significantly greater in animals that consumed Cd-contaminated food for two or three times. However, Cd accumulation in the liver started to appear after the first meal comprising the highest Cd concentration, while accumulation in the kidney appeared after the third feeding. It can be concluded that B. jararaca is a feasible model for biomonitoring Cd using the liver as a prognostic organ for the bioaccumulation as well as righting response and the damage index as indicators of Cd effects at an experimental level. Field monitoring feces stood out as the most suitable indicator for Cd ingestion by B. jararaca.
This study aimed to report the first occurrence of Leporacarus gibbus in a domestic rabbit (Oryct... more This study aimed to report the first occurrence of Leporacarus gibbus in a domestic rabbit (Oryctolagus cuniculus) from the state of Espírito Santo, Brazil. The two-year-old male rabbit came from a rural area and was admitted to the Veterinary Hospital Professor Ricardo Alexandre Hippler with intense pruritus throughout the body, severe hair loss, crustal lesions on the outer surface of the ears, and desquamation mainly in the dorsal region. A skin scraping and trichogram were performed. In the microscopic evaluation of the hairs obtained from the dorsal region, mites with morphology compatible with L. gibbus were observed. Cheyletiella parasitovorax and Psoropotes cuniculi were also detected in the same region. Treatment with ivermectin (0.4 mg/kg) was prescribed with three applications every 14 days, over a period of six weeks. This report presents the first description of the occurrence of L. gibbus in the state of Espírito Santo concomitant with poly-infestation of C. parasitovorax and P. cuniculi, and it is hoped that this will provide a research tool for future work in the region.
Revista de Patologia Tropical / Journal of Tropical Pathology, 2015
Platynosomum fastosum é um parasito de felinos, entre os quais os gatos domésticos, sobretudo os ... more Platynosomum fastosum é um parasito de felinos, entre os quais os gatos domésticos, sobretudo os originados de locais onde o clima é tropical e subtropical. Este é o parasito hepático mais comum de gatos domésticos e se localiza geralmente nas vias biliares e vesícula biliar, mas também pode ser encontrado no intestino delgado e pâncreas. A infecção se dá por meio da ingestão de lagartixa contendo as metacercárias que, nas vias biliares de gatos domésticos, causam uma colangite. O objetivo do presente trabalho foi relatar a ocorrência de P. fastosum, verificada por meio de necropsia de um gato doméstico, assintomático, com histórico de morte súbita, recebido no setor de Patologia Animal do Hospital Veterinário Ricardo Alexandre Hippler, Espírito Santo, Brasil. No exame macroscópico, foram observados exemplares adultos de P. fastosum no interior dos ductos biliares que se encontravam visivelmente dilatados. No exame histopatológico, foram confirmadas dilatação, presença de parasitos ...
Dogs produce fewer sperm than other species. Furthermore, for French Bulldogs, anatomical peculia... more Dogs produce fewer sperm than other species. Furthermore, for French Bulldogs, anatomical peculiarities, low libido, and increased sensitivity to stress could cause further reductions in sperm count. The objective was to compare effects of semen collection at 24- versus 48-h intervals on semen quality of French Bulldogs. Five purebred French Bulldogs, 19 to 48 months old, were subjected to 5 semen collections (24 h apart). After a 30-day rest, collections were repeated, but the interval between collections was 48 h. Semen was collected (all 3 fractions) by digital manipulation without female stimuli. Volume was measured in a 20-mL syringe. Sperm concentration was determined with a Neubauer counting chamber. Motility and vigor were evaluated with a coverslipped drop of semen on a slide (preheated to 37°C). Motility was expressed as a percentage of motile sperm, and vigor was classified on a scale of 1 to 5. Morphology was evaluated by the panoptic method; 100 cells were counted and r...
The intrinsic yield of spermatogenesis and the supporting indexes of the Sertoli cells are the be... more The intrinsic yield of spermatogenesis and the supporting indexes of the Sertoli cells are the best indicators for the spermatic production capacity in a species. The aim of the present study was to quantify the intrinsic yield of the spermatogenetic process, as well as the Sertoli cell index and spermatic reserves. Testicular fragments of five adult African lions was fixed in 4% glutaric aldehyde, dehydrated at increasing alcohol concentrations, included into hydroxyethyl methacrylate, and were cut into 4 microm thickness. In the seminiferous epithelium of the African lions, 10.3 primary spermatocytes at pre-leptotene phase are produced by the type-A spermatogonia. During meiotic divisions, only 2.7 spermatids were produced from the primary spermatocytes. The general spermatogenesis production in the African lions was approximately 22.1 cells, and each Sertoli cell was able to sustain and maintain approximately 14.9 cells of the germinative line, from which 7.9 are round spermatids. A total of 103x10(6) spermatozoa are produced by each testis gram at each cycle of the seminiferous epithelium. The spermatic reserve of lion is below the amplitude observed in mammals.
Puma or sussuarana (Puma concolor) is the second largest feline in the American continent and has... more Puma or sussuarana (Puma concolor) is the second largest feline in the American continent and has an ample latitudinal distribution in very diverse habitats. In relation to its conservation status, the puma is considered an extinction-threatened species. The study of the testis morphology and the spermatogenic process in a species is fundamental for establishing the physiologic patterns that will make possible the selection of the protocols for assisted reproduction. A number of peculiarities associated with the reproductive biology of specific species such as the duration of spermatogenic process can be used to determine the frequency of sperm collection. Nine adult male pumas maintained in captivity were used to determine the relative frequency of stages in the seminiferous epithelium cycle. Three of them received intra-testicular injections of 0.1 ml tritiated thymidine to determine the duration of the seminiferous epithelium cycle, and were subjected to biopsy 7 days later. The cycle of the seminiferous epithelium in puma was didactically described into eight stages by the tubular morphology method. The total duration of one seminiferous epithelium cycle in puma was calculated to be 9.89 days, and approximately 44.5 days are required for development of spermatozoon from spermatogonia. The duration of spermiogenesis, prophase and other events of meiosis were 14.08,
Six adult Leopardus tigrinus (oncilla) were studied to characterize stages of the seminiferous ep... more Six adult Leopardus tigrinus (oncilla) were studied to characterize stages of the seminiferous epithelium cycle and its relative frequency and duration, as well as morphometric parameters of the testes. Testicular fragments were obtained (incisional biopsy), embedded (glycol methacrylate), and histologic sections examined with light microscopy. The cycle of the seminiferous epithelium was categorized into eight stages (based on the tubular morphology method). The duration of one seminiferous epithelium cycle was 9.19 d, and approximately 41.37 d were required for development of sperm from spermatogonia. On average, diameter of the seminiferous tubules was 228.29 m, epithelium height was 78.86 m, and there were 16.99 m of testicular tubules per gram of testis. Body weight averaged 2.589 kg, of which 0.06 and 0.04% were attributed to the testis and seminiferous tubules, respectively. In conclusion, there were eight distinct stages in the seminiferous epithelium, the length of the seminiferous epithelium cycle was close to that in domestic cats and cougars, and testicular and somatic indexes were similar to those of other carnivores of similar size.
Causes of dental infections can be related to failed dental eruption, malocclusion, abrasion, fra... more Causes of dental infections can be related to failed dental eruption, malocclusion, abrasion, fractures with or without exposure of the dental pulp, and periodontal disease. Reports of oral myiasis in megavertebrates in captivity are infrequent, perhaps due to the difficulty in observing the oral cavity in such species. This report describes a case of oral myiasis in an adult male hippopotamus in the gingival area and alveolar mucosa of the left mandibular canine tooth.
Abstract: Quantitative computed tomography (QCT) is a highly sensitive, applicable technique for... more Abstract: Quantitative computed tomography (QCT) is a highly sensitive, applicable technique for determining the x-ray attenuation of organs. This technique reveals great precision in the detection of alterations in the x-ray attenuation of hepatic parenchyma, although the lack of studies establishing normal values limits its application in wild animals. The objective of this study was to establish mean hepatic attenuation values in four healthy juvenile sea turtles (Chelonia mydas) using QCT. Helical computed tomography scans were performed and regions of interest selected in the liver after multi-planar reconstruction images were obtained. The mean attenuation value for the hepatic parenchyma in these four turtles was 60.09 ± 5.3 standard deviation Hounsfield units. Determining normal x-ray attenuation values of the liver increases knowledge of the computed tomographic anatomy of this species and may be useful in the investigation of hepatic diseases.
Pathological and parasitological characterization of Prosthenorchis elegans in a free-ranging mar... more Pathological and parasitological characterization of Prosthenorchis elegans in a free-ranging marmoset Callithrix geofroyi from the Brazilian Atlantic Forest.
The endocrine portion of mammal testicle is represented by Leydig cells which, together with conn... more The endocrine portion of mammal testicle is represented by Leydig cells which, together with connective cells, leukocytes, blood and lymphatic vessels, form the intertubular space. The arrangement and proportion of these components vary in the different species of mammals and form mechanisms that keep the testosterone level--the main product of the Leydig cell--two to three times higher in the interstitial fluid than in the testicular blood vessels and 40-250 times higher in these than in the peripheral blood. Marked differences are observed among animal species regarding the abundance of Leydig cells, loose connective tissue, development degree and location of the lymphatic vessels and their topographical relationship with seminiferous tubules. In the jaguar about 13% of the testicular parenchyma is occupied by Leydig cells, 8.3% by connective tissue and 0.3% by lymphatic vessels. Although included in standard II, as described in the literature, concerning the arrangement of the intertubular space, the jaguar has grouped lymphatic vessels in the intertubular space instead of isolated ones. In the jaguar the average volume of the Leydig cell was 2386 microm3 and its average nuclear diameter was 7.7 microm. A great quantity of 2.3 microm diameter lipidic drops was observed in the Leydig cell cytoplasm of the jaguar. The Leydig cells in the jaguar occupy an average 0.0036% of the body weight and the average number per gram of testicle was within the range for most mammals: between 20 and 40 million.
O objetivo deste trabalho foi desenvolver um protocolo de extração hormonal à partir de fezes de ... more O objetivo deste trabalho foi desenvolver um protocolo de extração hormonal à partir de fezes de onça-pintada (Panthera onca) e validar uma metodologia de análise destes extratos por eletroforese capilar, verificando a eficiência analítica desta técnica no estudo dos metabólitos de esteróides sexuais em fezes, com o intuito de utilização futura desta metodologia na rotina dos laboratórios de endocrinologia. Foram testados sete tratamentos para as amostras de fezes liofilizadas: quatro protocolos de extração em diferentes solventes, um de hidrólise ácida e dois protocolos de extração em fase sólida (SPE). O protocolo de extração em acetonitrila foi satisfatório como pré-tratamento da amostra e aliado à SPE C-18 apresentou bons resultados no que diz respeito à sensibilidade, precisão, recuperação e tempo de análise. Com base nos resultados obtidos, a eletroforese capilar pode ser considerada uma ótima alternativa para a prospecção de metabólitos de hormônios, podendo ser utilizada em ...
Six adult Leopardus tigrinus (oncilla) were studied to characterize stages of the seminiferous ep... more Six adult Leopardus tigrinus (oncilla) were studied to characterize stages of the seminiferous epithelium cycle and its relative frequency and duration, as well as morphometric parameters of the testes. Testicular fragments were obtained (incisional biopsy), embedded (glycol methacrylate), and histologic sections examined with light microscopy. The cycle of the seminiferous epithelium was categorized into eight stages (based on the tubular morphology method). The duration of one seminiferous epithelium cycle was 9.19 d, and approximately 41.37 d were required for development of sperm from spermatogonia. On average, diameter of the seminiferous tubules was 228.29 m, epithelium height was 78.86 m, and there were 16.99 m of testicular tubules per gram of testis. Body weight averaged 2.589 kg, of which 0.06 and 0.04% were attributed to the testis and seminiferous tubules, respectively. In conclusion, there were eight distinct stages in the seminiferous epithelium, the length of the seminiferous epithelium cycle was close to that in domestic cats and cougars, and testicular and somatic indexes were similar to those of other carnivores of similar size.
Densitometria da vértebra dorsal, osso pleural e osso neural em tartarugas verdes hígidas por tom... more Densitometria da vértebra dorsal, osso pleural e osso neural em tartarugas verdes hígidas por tomografia computadorizada quantitativa Densitometry of dorsal vertebrae, pleural bone and neural bone in healthy green sea turtles by quantitative computed tomography Recebido para publicação 01.09.11 Aprovado em 04.03.12 Devolvido pelo autor 07.06.012 CR-5943 Palavras-chave: quelônios, coluna, densidade mineral óssea, tomografia computadorizada quantitativa, Chelonia mydas. ABSTRACT Quantitative computed tomography (QCT) is a technique with high sensitivity, applicability and accuracy in the diagnosis of bone loss; however the lack of normal values for wild animals limits their clinical application. This study aims to establish medium values radiodensity trabecular bone of the dorsal vertebrae, bone pleural and neural bone in healthy juvenile sea turtles using the QTC. For this we used an experimental group of five animals that underwent helical computed tomography, and measurements of bone radiodensity conducted at the level of the third, fourth and fifth dorsal vertebrae. The medium values of radiodensity trabecular bone of the dorsal vertebrae, pleural bone, and neural bone were respectively 287.4±39.1, 1093.74±100.4, and 1089.6±86.5 Hounsfield units (HU). Further studies should be performed in different species and ages of turtles to encourage the clinical application of this technique.
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