Alzheimer's disease (AD) is a progressive neurodegenerative disease. This study was performed to ... more Alzheimer's disease (AD) is a progressive neurodegenerative disease. This study was performed to determine the possible relationship between melatonin, which is known to play a role in the neuro-protective mechanism in AD, and fasciculation and elongation protein zeta 1 (FEZ1). Thirty male rats were included and separated into 3 groups (n = 10) as vehicle (artifi cial cerebrospinal fl uid), streptozotocin (STZ) and STZ+melatonin (MLT). Two intracerebroventricular (icv) injections of 3 mg/kg STZ were made 48 hours apart. MLT injections were implemented for 14 days (ip; 10mg/kg/day). The Morris Water Maze (MWM) test was performed and rats were sacrifi ced to assess FEZ1 gene expression and protein levels from the hippocampus tissues and serum levels of noradrenaline (NA), dopamine and serotonin were determined from the blood samples. It was determined that the FEZ1/β-actin protein ratio in the STZ group was signifi cantly higher than that of the Vehicle group (p < 0.05) and in the MLT-administered group, the protein levels were decreased to the levels observed in the Vehicle group. Serum NA levels of STZ and STZ+MLT groups were found to be lower than those in the Vehicle group, while no difference was found regarding dopamine and serotonin levels. These fi ndings show that reversal of increased FEZ1 levels in AD-induced rats with melatonin administration is the evidence of the effect of melatonin through FEZ1 in AD (Tab. 2, Fig. 5, Ref. 67).
The effects of different fluid resuscitations in the acute phase of combined traumatic brain injury and hemorrhagic shock in an experimental rat model
Medicine Science | International Medical Journal, 2021
It was aimed to investigate the utility of different fluid replacement therapies in the acute pha... more It was aimed to investigate the utility of different fluid replacement therapies in the acute phase of a combined experimental traumatic brain injury and hemorrhagic shock model in terms of biochemical, hemostatic and pathological changes in the brain. 48 rats were divided up into 6 groups (n=8). Control group (S) rats were subjected to sham experimental hemorrhagic shock after which they underwent a sham operation, while trauma group (T) rats were subjected to hemorrhagic shock and subsequent head trauma with no treatment. Among the rats subjected to hemorrhagic shock and subsequent head trauma, those given 3% NaCl after this were named as HS group; those given HyperHeas [7.2% NaCl / 6% poly (O-2-Hydroxyethyl) starch] were named as HyperHS group; those given 0.9% NaCl were determined as NS group and those given ringer lactate as RL group. 24-hours later, the fluids’ effects were evaluated. The brain fluid content and INR levels were significantly higher in all the experimental groups when set against those in the NS group (p ranged <0.01 to <0.001). aPTT was significantly longer in the T and HS groups than in that seen in the NS group (p<0.001 for each). Rats in the HS and RL groups showed significantly more bleeding than those in the NS group (p<0.05). Of the treatment groups, the HyperHS group had more brain edema when compared to the NS and RL groups (p<0.05). The proportion of red neuron and necrosis was partially decreased in the treatment groups, with no significant difference determined between the HS, HyperHS, NS and RL groups (p>0.05). In conclusion, study findings support the safely use of normal saline and Ringer lactate solutions in prompt fluid resuscitation where both traumatic brain injury and hemorrhagic shock have occurred, based on the overall advantages pertaining to each critical prognostic parameter.
Irisin infusion increased food intake in rats. Irisin infusion decreased the thyroid hormone ... more Irisin infusion increased food intake in rats. Irisin infusion decreased the thyroid hormone levels, but increased the body temperature. Infusion of irisin increased UCP1 in adipose tissues and UCP3 mRNA levels in muscle tissue.
To evaluate the asymmetric dimethylarginine (ADMA), symmetric dimethylarginine (SDMA) and L-argin... more To evaluate the asymmetric dimethylarginine (ADMA), symmetric dimethylarginine (SDMA) and L-arginine (L-Arg) levels both in plasma and aqueous humor of primary open angle glaucoma (POAG) patients and matched controls. Patients and Methods: 25 primary open angle glaucoma paients and 42 control cases with senile cataract were included in the study. Plasma and aqueous humor ADMA, SDMA and L-Arg levels of the participants were measured by using liquid chromatography-tandem mass spectrometry. Results: A significant increase in aqueous humor SDMA level was detected in POAG patients compared with controls (p=0.0115). No significant difference was detected in plasma and aqueous humor ADMA, L-Arg levels. The aqueous humor levels of SDMA is found to be associated with POAG. The result of this current study supports the role of nitric oxide pathway in glaucoma.
Resveratrol reduces light and electron microscopic changes in acetaminophen-induced hepatotoxicity in rats: Role of iNOS expression
Ultrastructural pathology
Hepatotoxicity is a major complication of acetaminophen (APAP), a widely used analgesic and antip... more Hepatotoxicity is a major complication of acetaminophen (APAP), a widely used analgesic and antipyretic drug. Resveratrol (RSV) is a naturally occurring diphenol and it has anticancer, antioxidant, and anti-inflammatory properties. In this study, the beneficial effects of RSV on APAP-induced hepatotoxicity was investigated in rats. Group 1: Ethanol, Group 2: Saline, Group 3: RSV (10 mg/kg/ip), Group 4: APAP (1000 mg/kg/ip/single dose), Group 5: APAP+RSV (20 min after administration of APAP). The rats were sacrificed 24 h after administration of APAP. Light and electron microscopic changes were evaluated. Levels of malondialdehyde (MDA) and glutathione (GSH), catalase (CAT), and superoxide dismutase (SOD) activities were determined in liver tissue. Rats of the ethanol, saline, and RSV groups did not present any histopathological alterations. In the APAP group, we observed vascular congestion, necrosis, inflammation, sinusoidal dilatation, and loss of glycogen content. In the APAP+RSV...
Salusin-α and salusin-β are expressed in many tissues including the central nervous system, vesse... more Salusin-α and salusin-β are expressed in many tissues including the central nervous system, vessels and kidneys; they have been shown to decrease endoplasmic reticulum stress during heart ischemia/ reperfusion (I/R) and to decrease apoptosis. We investigated the relation of salusin-α and salusin-β levels to acute ischemic renal failure. We also investigated whether these peptides are protective against renal I/R damage. Fifty-three rats were divided into six groups: control, I/R, I/R + salusin-α1, I/R + salusin-α10, I/R + salusin-β1 and I/R + salusin-β10. After removing the right kidney, the left kidney was subjected to ischemia for 1 h and reperfusion for 23 h. The treatment groups were injected subcutaneously at the beginning of ischemia with 1 or 10 μg/kg salusin-α, and 1 or 10 μg/kg salusin-β. Histopathology was assessed at the end of the experiment. Superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-PX) activity and malondialdehyde (MDA) levels were measured in the kidney tissue. Serum levels of blood urea nitrogen (BUN), creatinine (Cre), tumor necrosis factoralpha (TNF-α), interleukin-6 (IL-6) and interleukin-1 beta (IL-1β) also were measured. Levels of salusinα and salusin-β were measured in the serum and kidney tissues of the control and I/R groups. SOD, CAT and GSH-PX activities were decreased and the levels of MDA, TNF-α, IL-6, IL-1β, BUN and Cre were increased in the I/R group compared to controls. Severe glomerular and tubular damage was apparent in the I/R group compared to controls. The level of salusin-β was decreased in the serum and kidney tissue of the I/R group compared to controls, whereas the level of salusin-α was decreased in the serum and increased in the kidney tissue. Salusin-α and salusin-β administration increased SOD and GSH-PX enzyme activation and decreased the levels of MDA, TNF-α, IL-6 and IL-1β compared to the I/R group. BUN and Cre levels were decreased in the I/R + salusin-α1 group and the level of Cre was decreased in I/R + salusin-β10 group compared to the I/R group. We demonstrated a protective effect of salusin-α and salusin-β against renal I/R damage. Changes in the levels of salusin-α and salusin-β in the I/R group suggest that these peptides may be associated with acute renal failure.
A large body of work has implicated the translocation of enteric bacterial infections in many ser... more A large body of work has implicated the translocation of enteric bacterial infections in many serious conditions such as liver failure, hemorrhagic shock, burns, and acute pancreatitis (1-4). Many experimental studies have reported that intestinal oxidative stress plays a key role in the disruption of intestinal barrier function (5-7). Glucocorticoids are well-known anti-inflammatory agents and are commonly used as therapeutic agents for many acute and chronic inflammatory illnesses such as ulcer-ative colitis, Crohn' s disease, and autoimmune hepatitis. Steroids inhibit the expression of a variety of genes involved in the inflammation process, including cytokines and cellular adhesion molecules, and steroids have been reported to protect the intestine from oxidative stress in different models of intestinal damage (8-10). In this study, we investigated the effects of methylprednisolone (MP) on intestinal oxidative damage and bacterial translocation (BT) in thioacetamide (TAA)-induced liver failure in rats.
Comparison of the effects of Argon and Neodymium: YAG laser iridotomy on cytokines in the rabbit aqueous humor
European journal of ophthalmology
We compared the effects in rabbits of iridotomy using the argon or Nd:YAG laser on cytokines such... more We compared the effects in rabbits of iridotomy using the argon or Nd:YAG laser on cytokines such as interleukin 1-beta (IL-1b), interleukin 6 (IL-6), interleukin 8 (IL-8), and tumor necrosis factor-alpha (TNF-a). Twenty pigmented rabbits (20 eyes) underwent argon and Nd:YAG iridotomy under general and topical anesthesia. Group 1 (10 right eyes) was treated with the argon laser and group 2 (10 right eyes) with the Nd:YAG laser, using an equal number of shots and the same laser parameters for each group. Left eyes in both groups were evaluated as controls. Aqueous humor specimens were collected from each eye preoperatively and 1 and 3 days postoperatively. Aqueous IL-6 and TNF-a levels rose more on day 1 in the Nd:YAG group than the argon group. IL-6 and TNF-a levels were significantly higher on days 1 and 3 than the preoperative and control levels (p<0.05). TNF-a levels on day 1 were significantly lower in the Nd:YAG than the argon group. There were no significant differences bet...
Effects of apelin on reproductive functions: relationship with feeding behavior and energy metabolism
Archives of Physiology and Biochemistry, 2016
Apelin is an adipose tissue derived peptidergic hormone. In this study, 40 male Sprague-Dawley ra... more Apelin is an adipose tissue derived peptidergic hormone. In this study, 40 male Sprague-Dawley rats were used (four groups; n = 10). Apelin-13 at three different dosages (1, 5 and 50 μg/kg) was given intraperitoneally while the control group received vehicle the same route for a period of 14 days. In results, apelin-13 caused significant decreases in serum testosterone, luteinizing hormone and follicle-stimulating hormone levels (p &amp;amp;amp;amp;amp;amp;lt; 0.05). Administration of apelin-13 significantly increased body weights, food intake, serum low-density lipoprotein and total cholesterol levels (p &amp;amp;amp;amp;amp;amp;lt; 0.05), but caused significant decreases in high-density lipoprotein levels (p &amp;amp;amp;amp;amp;amp;lt; 0.05). Serum glucose and triglyceride levels were not significantly altered by apelin-13 administration. Significant decreases in both uncoupling protein (UCP)-1 levels in the white and brown adipose tissues and UCP-3 levels in the biceps muscle (p &amp;amp;amp;amp;amp;amp;lt; 0.05) were noted. The findings of the study suggest that apelin-13 may not only lead to obesity by increasing body weight but also cause infertility by suppressing reproductive hormones.
The aim of the present study was to clarify the role of oxidative stress in streptozotocin induce... more The aim of the present study was to clarify the role of oxidative stress in streptozotocin induced liver injury and the possible protective effect of caffeic acid phenethyl ester (CAPE) using histological and biochemical parameters. 32 male Wistar rats were divided into 4 groups as follows: Group 1: Control animals, Group 2: Control animals given CAPE Group 3: STZ-induced diabetic animals (DM group), Group 4: STZ-induced diabetic rats given CAPE (DM+CAPE group). All the injections started on the same day of single-dose STZ injection and continued for 20 days. At the end of this period, livers were removed and processed for routine histological procedures. Biochemical parameters and morphological changes were examined. In DM group, blood glucose levels were signifi cantly increased compared with the control group. Signifi cant increases in tissue malondialdehyde (MDA) level and decreases in superoxide dismutase (SOD) and total glutathione (GSH) activities were detected in DM group. Administration of CAPE signifi cantly reduced these values. STZ-induced histopathological alterations including infl ammatory cell infi ltration around portal triad, congestion, loss of glycogen in the hepatocytes. Additionally, degenerative cellular alterations, such as numerous vacuolizations including myelinic fi gure formation, pyknotic nuclei with peripheral localization of heterochromatin condensation and mitochondrial elongation were observed in cytoplasm of hepatocytes. CAPE signifi cantly reduced these histopathological changes. Our results indicate that CAPE should be considered in the prevention of oxidative stress in diabetic liver (Tab. 3, Fig. 4, Ref. 47).
Medicine Science | International Medical Journal, 2016
Our study aim was to measure the urinary N-acetyl-β-D-glucosaminidase/creatinine (NAG/UCr) index ... more Our study aim was to measure the urinary N-acetyl-β-D-glucosaminidase/creatinine (NAG/UCr) index in epileptic children who received levetiracetam (LEV) treatment at least for 6 months, and compare it to healthy children. Thirty five children with epilepsy were enrolled in this prospective study. NAG was studied using the calorimetric method and NAG levels were expressed in units per liter (U/L) and NAG/UCre levels were determined in U/mmol creatinine. There were no statistically significant differences for the urine NAG and NAG/UCr index before and after LEV treatment in the epileptic group (p>0.05, for each). There were no significant correlations between the serum concentration of LEV and urinary NAG levels (r=0.258, p=0.135) and NAG/UCr levels (r=0.164, p=0.346) before treatment. Our study demonstrated that LEV treatment was safe and did not interfere in renal tubular function in epileptic children.
Effects of central apelin-13 infusion on the hypothalamic TRH mRNA and serum TSH levels in rats
Objective: Apelin is a peptide hormone known as the ligand for the G protein-coupled APJ receptor... more Objective: Apelin is a peptide hormone known as the ligand for the G protein-coupled APJ receptor. Apelin and APJ mediate a wide range of physiological actions including regulation of energy metabolism, food intake and fluid homeostasis. In our previous study, we had shown that the intracerebroventricular infusion of apelin-13 decreased serum free T3 and T4 levels. These results suggest that apelin-13 may play a role in the hypothalamus-hypophysis-thyroid axis. This study was designed to determine changes in the hypothalamic TRH mRNA and serum TSH levels after intracerebroventricular apelin-13 infusion. Methods: In this study, 40 Sprague Dawley male rats were used, and were divided randomly into four groups (n=10). The rats in the control group were not applied any surgical procedure. Sham and experiment groups received continually intracerebroventricular infusion via osmotic mini pump filled artificial cerebrospinal fluid or apelin-13 at concentration of 1 and 10 nmol (10ul/h) for ...
Association of folic acid receptor α in maternal serum with neural tube defects
The Journal of Maternal-Fetal & Neonatal Medicine, 2013
To evaluate whether serum folic receptor α levels are changed in women whose previous pregnancies... more To evaluate whether serum folic receptor α levels are changed in women whose previous pregnancies were complicated with neural tube defects (NTDs). This was a case-control study that included 41 women as the control group who had previously had at least one healthy pregnancy and 37 women as the study group who had a previous pregnancy complicated with NTDs. Blood samples were obtained from all of the participants six weeks after the termination of pregnancy or delivery of a baby. Serum folate receptor α concentrations were analyzed using a commercially available enzyme-linked immunosorbent assay (ELISA) kit. The mean concentrations of serum folate receptor α were significantly lower in the NTD cases compared to those in the control group (p = 0.02). There was no significant difference in mean serum folate titers between the NTD cases and the control group (p = 0.07). Low serum folic acid receptor α levels in the current study did not appear to be a regulatory marker of maternal folate homeostasis per se but rather a factor that contributed to the development of NTDs.
Serum Leptin, Resistin, and Lipid Levels in Patients with End Stage Renal Failure with Regard to Dialysis Modality
Renal Failure, 2007
Little information is available on the relationship between serum resistin levels and other adipo... more Little information is available on the relationship between serum resistin levels and other adipokines with serum lipid levels and insulin resistance in uremic patients under different dialysis modalities. This study investigated the effects of dialysis modality on serum leptin, adiponectin, resistin, interleukin 6 (IL-6), and tumor necrosis factor (TNF) alpha levels in age, sex, and total adipose tissue mass (TATM); matched 30 hemodialysis (HD) patients, 30 continuous peritoneal dialysis (CAPD) patients, and 30 healthy controls; and evaluated the relationship between these adipokines and dyslipidemia and insulin resistance. Serum resistin, adiponectin, IL-6, TNF-alpha, and high sensitive C reactive protein (hsCRP) levels were significantly increased in dialysis patients compared to controls (p &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt; 0.05). In CAPD patients, serum leptin, resistin, triglycerides, and total cholesterol levels were higher than those in HD patients (p &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt; 0.05). Leptin levels were positively correlated with TATM, serum triglycerides, total cholesterol, and low density lipoprotein (LDLc) levels in both dialysis groups. Resistin levels were found to positively correlate with TATM and triglycerides in CAPD patients. No relationship was found between the homeostasis model assessment-insulin resistance index (HOMA-IR) and adipokines studied. Serum leptin, resistin, triglycerides, and total cholesterol levels were higher in CAPD patients. Leptin levels were positively correlated with TATM, serum triglycerides, total cholesterol, and LDLc levels in dialysis patients. Resistin levels were positively correlated with TATM and triglycerides in CAPD patients. Glucose load during CAPD may be an important factor in increased in leptin, resistin, triglycerides, and total cholesterol levels in CAPD patients. These results highlight the importance of leptin and resistin as determinants of dyslipidemia, especially in CAPD patients.
Letter To The Editor: “Relationship between Insulin Resistance and Malondialdehyde Levels in Dialysis Patients”
Renal Failure, 2007
1. Ren Fail. 2007;29(5):649-51. Relationship between insulin resistance and malondialdehyde level... more 1. Ren Fail. 2007;29(5):649-51. Relationship between insulin resistance and malondialdehyde levels in dialysis patients. Taskapan MC, Keskin L, Sahin I, Ozyalin F, Atmaca H, Temel I, Taskapan H. PMID: 17654332 [PubMed - indexed for MEDLINE]. Publication Types: Letter. ...
Alzheimer's disease (AD) is a progressive neurodegenerative disease. This study was performed to ... more Alzheimer's disease (AD) is a progressive neurodegenerative disease. This study was performed to determine the possible relationship between melatonin, which is known to play a role in the neuro-protective mechanism in AD, and fasciculation and elongation protein zeta 1 (FEZ1). Thirty male rats were included and separated into 3 groups (n = 10) as vehicle (artifi cial cerebrospinal fl uid), streptozotocin (STZ) and STZ+melatonin (MLT). Two intracerebroventricular (icv) injections of 3 mg/kg STZ were made 48 hours apart. MLT injections were implemented for 14 days (ip; 10mg/kg/day). The Morris Water Maze (MWM) test was performed and rats were sacrifi ced to assess FEZ1 gene expression and protein levels from the hippocampus tissues and serum levels of noradrenaline (NA), dopamine and serotonin were determined from the blood samples. It was determined that the FEZ1/β-actin protein ratio in the STZ group was signifi cantly higher than that of the Vehicle group (p < 0.05) and in the MLT-administered group, the protein levels were decreased to the levels observed in the Vehicle group. Serum NA levels of STZ and STZ+MLT groups were found to be lower than those in the Vehicle group, while no difference was found regarding dopamine and serotonin levels. These fi ndings show that reversal of increased FEZ1 levels in AD-induced rats with melatonin administration is the evidence of the effect of melatonin through FEZ1 in AD (Tab. 2, Fig. 5, Ref. 67).
The effects of different fluid resuscitations in the acute phase of combined traumatic brain injury and hemorrhagic shock in an experimental rat model
Medicine Science | International Medical Journal, 2021
It was aimed to investigate the utility of different fluid replacement therapies in the acute pha... more It was aimed to investigate the utility of different fluid replacement therapies in the acute phase of a combined experimental traumatic brain injury and hemorrhagic shock model in terms of biochemical, hemostatic and pathological changes in the brain. 48 rats were divided up into 6 groups (n=8). Control group (S) rats were subjected to sham experimental hemorrhagic shock after which they underwent a sham operation, while trauma group (T) rats were subjected to hemorrhagic shock and subsequent head trauma with no treatment. Among the rats subjected to hemorrhagic shock and subsequent head trauma, those given 3% NaCl after this were named as HS group; those given HyperHeas [7.2% NaCl / 6% poly (O-2-Hydroxyethyl) starch] were named as HyperHS group; those given 0.9% NaCl were determined as NS group and those given ringer lactate as RL group. 24-hours later, the fluids’ effects were evaluated. The brain fluid content and INR levels were significantly higher in all the experimental groups when set against those in the NS group (p ranged <0.01 to <0.001). aPTT was significantly longer in the T and HS groups than in that seen in the NS group (p<0.001 for each). Rats in the HS and RL groups showed significantly more bleeding than those in the NS group (p<0.05). Of the treatment groups, the HyperHS group had more brain edema when compared to the NS and RL groups (p<0.05). The proportion of red neuron and necrosis was partially decreased in the treatment groups, with no significant difference determined between the HS, HyperHS, NS and RL groups (p>0.05). In conclusion, study findings support the safely use of normal saline and Ringer lactate solutions in prompt fluid resuscitation where both traumatic brain injury and hemorrhagic shock have occurred, based on the overall advantages pertaining to each critical prognostic parameter.
Irisin infusion increased food intake in rats. Irisin infusion decreased the thyroid hormone ... more Irisin infusion increased food intake in rats. Irisin infusion decreased the thyroid hormone levels, but increased the body temperature. Infusion of irisin increased UCP1 in adipose tissues and UCP3 mRNA levels in muscle tissue.
To evaluate the asymmetric dimethylarginine (ADMA), symmetric dimethylarginine (SDMA) and L-argin... more To evaluate the asymmetric dimethylarginine (ADMA), symmetric dimethylarginine (SDMA) and L-arginine (L-Arg) levels both in plasma and aqueous humor of primary open angle glaucoma (POAG) patients and matched controls. Patients and Methods: 25 primary open angle glaucoma paients and 42 control cases with senile cataract were included in the study. Plasma and aqueous humor ADMA, SDMA and L-Arg levels of the participants were measured by using liquid chromatography-tandem mass spectrometry. Results: A significant increase in aqueous humor SDMA level was detected in POAG patients compared with controls (p=0.0115). No significant difference was detected in plasma and aqueous humor ADMA, L-Arg levels. The aqueous humor levels of SDMA is found to be associated with POAG. The result of this current study supports the role of nitric oxide pathway in glaucoma.
Resveratrol reduces light and electron microscopic changes in acetaminophen-induced hepatotoxicity in rats: Role of iNOS expression
Ultrastructural pathology
Hepatotoxicity is a major complication of acetaminophen (APAP), a widely used analgesic and antip... more Hepatotoxicity is a major complication of acetaminophen (APAP), a widely used analgesic and antipyretic drug. Resveratrol (RSV) is a naturally occurring diphenol and it has anticancer, antioxidant, and anti-inflammatory properties. In this study, the beneficial effects of RSV on APAP-induced hepatotoxicity was investigated in rats. Group 1: Ethanol, Group 2: Saline, Group 3: RSV (10 mg/kg/ip), Group 4: APAP (1000 mg/kg/ip/single dose), Group 5: APAP+RSV (20 min after administration of APAP). The rats were sacrificed 24 h after administration of APAP. Light and electron microscopic changes were evaluated. Levels of malondialdehyde (MDA) and glutathione (GSH), catalase (CAT), and superoxide dismutase (SOD) activities were determined in liver tissue. Rats of the ethanol, saline, and RSV groups did not present any histopathological alterations. In the APAP group, we observed vascular congestion, necrosis, inflammation, sinusoidal dilatation, and loss of glycogen content. In the APAP+RSV...
Salusin-α and salusin-β are expressed in many tissues including the central nervous system, vesse... more Salusin-α and salusin-β are expressed in many tissues including the central nervous system, vessels and kidneys; they have been shown to decrease endoplasmic reticulum stress during heart ischemia/ reperfusion (I/R) and to decrease apoptosis. We investigated the relation of salusin-α and salusin-β levels to acute ischemic renal failure. We also investigated whether these peptides are protective against renal I/R damage. Fifty-three rats were divided into six groups: control, I/R, I/R + salusin-α1, I/R + salusin-α10, I/R + salusin-β1 and I/R + salusin-β10. After removing the right kidney, the left kidney was subjected to ischemia for 1 h and reperfusion for 23 h. The treatment groups were injected subcutaneously at the beginning of ischemia with 1 or 10 μg/kg salusin-α, and 1 or 10 μg/kg salusin-β. Histopathology was assessed at the end of the experiment. Superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-PX) activity and malondialdehyde (MDA) levels were measured in the kidney tissue. Serum levels of blood urea nitrogen (BUN), creatinine (Cre), tumor necrosis factoralpha (TNF-α), interleukin-6 (IL-6) and interleukin-1 beta (IL-1β) also were measured. Levels of salusinα and salusin-β were measured in the serum and kidney tissues of the control and I/R groups. SOD, CAT and GSH-PX activities were decreased and the levels of MDA, TNF-α, IL-6, IL-1β, BUN and Cre were increased in the I/R group compared to controls. Severe glomerular and tubular damage was apparent in the I/R group compared to controls. The level of salusin-β was decreased in the serum and kidney tissue of the I/R group compared to controls, whereas the level of salusin-α was decreased in the serum and increased in the kidney tissue. Salusin-α and salusin-β administration increased SOD and GSH-PX enzyme activation and decreased the levels of MDA, TNF-α, IL-6 and IL-1β compared to the I/R group. BUN and Cre levels were decreased in the I/R + salusin-α1 group and the level of Cre was decreased in I/R + salusin-β10 group compared to the I/R group. We demonstrated a protective effect of salusin-α and salusin-β against renal I/R damage. Changes in the levels of salusin-α and salusin-β in the I/R group suggest that these peptides may be associated with acute renal failure.
A large body of work has implicated the translocation of enteric bacterial infections in many ser... more A large body of work has implicated the translocation of enteric bacterial infections in many serious conditions such as liver failure, hemorrhagic shock, burns, and acute pancreatitis (1-4). Many experimental studies have reported that intestinal oxidative stress plays a key role in the disruption of intestinal barrier function (5-7). Glucocorticoids are well-known anti-inflammatory agents and are commonly used as therapeutic agents for many acute and chronic inflammatory illnesses such as ulcer-ative colitis, Crohn' s disease, and autoimmune hepatitis. Steroids inhibit the expression of a variety of genes involved in the inflammation process, including cytokines and cellular adhesion molecules, and steroids have been reported to protect the intestine from oxidative stress in different models of intestinal damage (8-10). In this study, we investigated the effects of methylprednisolone (MP) on intestinal oxidative damage and bacterial translocation (BT) in thioacetamide (TAA)-induced liver failure in rats.
Comparison of the effects of Argon and Neodymium: YAG laser iridotomy on cytokines in the rabbit aqueous humor
European journal of ophthalmology
We compared the effects in rabbits of iridotomy using the argon or Nd:YAG laser on cytokines such... more We compared the effects in rabbits of iridotomy using the argon or Nd:YAG laser on cytokines such as interleukin 1-beta (IL-1b), interleukin 6 (IL-6), interleukin 8 (IL-8), and tumor necrosis factor-alpha (TNF-a). Twenty pigmented rabbits (20 eyes) underwent argon and Nd:YAG iridotomy under general and topical anesthesia. Group 1 (10 right eyes) was treated with the argon laser and group 2 (10 right eyes) with the Nd:YAG laser, using an equal number of shots and the same laser parameters for each group. Left eyes in both groups were evaluated as controls. Aqueous humor specimens were collected from each eye preoperatively and 1 and 3 days postoperatively. Aqueous IL-6 and TNF-a levels rose more on day 1 in the Nd:YAG group than the argon group. IL-6 and TNF-a levels were significantly higher on days 1 and 3 than the preoperative and control levels (p<0.05). TNF-a levels on day 1 were significantly lower in the Nd:YAG than the argon group. There were no significant differences bet...
Effects of apelin on reproductive functions: relationship with feeding behavior and energy metabolism
Archives of Physiology and Biochemistry, 2016
Apelin is an adipose tissue derived peptidergic hormone. In this study, 40 male Sprague-Dawley ra... more Apelin is an adipose tissue derived peptidergic hormone. In this study, 40 male Sprague-Dawley rats were used (four groups; n = 10). Apelin-13 at three different dosages (1, 5 and 50 μg/kg) was given intraperitoneally while the control group received vehicle the same route for a period of 14 days. In results, apelin-13 caused significant decreases in serum testosterone, luteinizing hormone and follicle-stimulating hormone levels (p &amp;amp;amp;amp;amp;amp;lt; 0.05). Administration of apelin-13 significantly increased body weights, food intake, serum low-density lipoprotein and total cholesterol levels (p &amp;amp;amp;amp;amp;amp;lt; 0.05), but caused significant decreases in high-density lipoprotein levels (p &amp;amp;amp;amp;amp;amp;lt; 0.05). Serum glucose and triglyceride levels were not significantly altered by apelin-13 administration. Significant decreases in both uncoupling protein (UCP)-1 levels in the white and brown adipose tissues and UCP-3 levels in the biceps muscle (p &amp;amp;amp;amp;amp;amp;lt; 0.05) were noted. The findings of the study suggest that apelin-13 may not only lead to obesity by increasing body weight but also cause infertility by suppressing reproductive hormones.
The aim of the present study was to clarify the role of oxidative stress in streptozotocin induce... more The aim of the present study was to clarify the role of oxidative stress in streptozotocin induced liver injury and the possible protective effect of caffeic acid phenethyl ester (CAPE) using histological and biochemical parameters. 32 male Wistar rats were divided into 4 groups as follows: Group 1: Control animals, Group 2: Control animals given CAPE Group 3: STZ-induced diabetic animals (DM group), Group 4: STZ-induced diabetic rats given CAPE (DM+CAPE group). All the injections started on the same day of single-dose STZ injection and continued for 20 days. At the end of this period, livers were removed and processed for routine histological procedures. Biochemical parameters and morphological changes were examined. In DM group, blood glucose levels were signifi cantly increased compared with the control group. Signifi cant increases in tissue malondialdehyde (MDA) level and decreases in superoxide dismutase (SOD) and total glutathione (GSH) activities were detected in DM group. Administration of CAPE signifi cantly reduced these values. STZ-induced histopathological alterations including infl ammatory cell infi ltration around portal triad, congestion, loss of glycogen in the hepatocytes. Additionally, degenerative cellular alterations, such as numerous vacuolizations including myelinic fi gure formation, pyknotic nuclei with peripheral localization of heterochromatin condensation and mitochondrial elongation were observed in cytoplasm of hepatocytes. CAPE signifi cantly reduced these histopathological changes. Our results indicate that CAPE should be considered in the prevention of oxidative stress in diabetic liver (Tab. 3, Fig. 4, Ref. 47).
Medicine Science | International Medical Journal, 2016
Our study aim was to measure the urinary N-acetyl-β-D-glucosaminidase/creatinine (NAG/UCr) index ... more Our study aim was to measure the urinary N-acetyl-β-D-glucosaminidase/creatinine (NAG/UCr) index in epileptic children who received levetiracetam (LEV) treatment at least for 6 months, and compare it to healthy children. Thirty five children with epilepsy were enrolled in this prospective study. NAG was studied using the calorimetric method and NAG levels were expressed in units per liter (U/L) and NAG/UCre levels were determined in U/mmol creatinine. There were no statistically significant differences for the urine NAG and NAG/UCr index before and after LEV treatment in the epileptic group (p>0.05, for each). There were no significant correlations between the serum concentration of LEV and urinary NAG levels (r=0.258, p=0.135) and NAG/UCr levels (r=0.164, p=0.346) before treatment. Our study demonstrated that LEV treatment was safe and did not interfere in renal tubular function in epileptic children.
Effects of central apelin-13 infusion on the hypothalamic TRH mRNA and serum TSH levels in rats
Objective: Apelin is a peptide hormone known as the ligand for the G protein-coupled APJ receptor... more Objective: Apelin is a peptide hormone known as the ligand for the G protein-coupled APJ receptor. Apelin and APJ mediate a wide range of physiological actions including regulation of energy metabolism, food intake and fluid homeostasis. In our previous study, we had shown that the intracerebroventricular infusion of apelin-13 decreased serum free T3 and T4 levels. These results suggest that apelin-13 may play a role in the hypothalamus-hypophysis-thyroid axis. This study was designed to determine changes in the hypothalamic TRH mRNA and serum TSH levels after intracerebroventricular apelin-13 infusion. Methods: In this study, 40 Sprague Dawley male rats were used, and were divided randomly into four groups (n=10). The rats in the control group were not applied any surgical procedure. Sham and experiment groups received continually intracerebroventricular infusion via osmotic mini pump filled artificial cerebrospinal fluid or apelin-13 at concentration of 1 and 10 nmol (10ul/h) for ...
Association of folic acid receptor α in maternal serum with neural tube defects
The Journal of Maternal-Fetal & Neonatal Medicine, 2013
To evaluate whether serum folic receptor α levels are changed in women whose previous pregnancies... more To evaluate whether serum folic receptor α levels are changed in women whose previous pregnancies were complicated with neural tube defects (NTDs). This was a case-control study that included 41 women as the control group who had previously had at least one healthy pregnancy and 37 women as the study group who had a previous pregnancy complicated with NTDs. Blood samples were obtained from all of the participants six weeks after the termination of pregnancy or delivery of a baby. Serum folate receptor α concentrations were analyzed using a commercially available enzyme-linked immunosorbent assay (ELISA) kit. The mean concentrations of serum folate receptor α were significantly lower in the NTD cases compared to those in the control group (p = 0.02). There was no significant difference in mean serum folate titers between the NTD cases and the control group (p = 0.07). Low serum folic acid receptor α levels in the current study did not appear to be a regulatory marker of maternal folate homeostasis per se but rather a factor that contributed to the development of NTDs.
Serum Leptin, Resistin, and Lipid Levels in Patients with End Stage Renal Failure with Regard to Dialysis Modality
Renal Failure, 2007
Little information is available on the relationship between serum resistin levels and other adipo... more Little information is available on the relationship between serum resistin levels and other adipokines with serum lipid levels and insulin resistance in uremic patients under different dialysis modalities. This study investigated the effects of dialysis modality on serum leptin, adiponectin, resistin, interleukin 6 (IL-6), and tumor necrosis factor (TNF) alpha levels in age, sex, and total adipose tissue mass (TATM); matched 30 hemodialysis (HD) patients, 30 continuous peritoneal dialysis (CAPD) patients, and 30 healthy controls; and evaluated the relationship between these adipokines and dyslipidemia and insulin resistance. Serum resistin, adiponectin, IL-6, TNF-alpha, and high sensitive C reactive protein (hsCRP) levels were significantly increased in dialysis patients compared to controls (p &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt; 0.05). In CAPD patients, serum leptin, resistin, triglycerides, and total cholesterol levels were higher than those in HD patients (p &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt; 0.05). Leptin levels were positively correlated with TATM, serum triglycerides, total cholesterol, and low density lipoprotein (LDLc) levels in both dialysis groups. Resistin levels were found to positively correlate with TATM and triglycerides in CAPD patients. No relationship was found between the homeostasis model assessment-insulin resistance index (HOMA-IR) and adipokines studied. Serum leptin, resistin, triglycerides, and total cholesterol levels were higher in CAPD patients. Leptin levels were positively correlated with TATM, serum triglycerides, total cholesterol, and LDLc levels in dialysis patients. Resistin levels were positively correlated with TATM and triglycerides in CAPD patients. Glucose load during CAPD may be an important factor in increased in leptin, resistin, triglycerides, and total cholesterol levels in CAPD patients. These results highlight the importance of leptin and resistin as determinants of dyslipidemia, especially in CAPD patients.
Letter To The Editor: “Relationship between Insulin Resistance and Malondialdehyde Levels in Dialysis Patients”
Renal Failure, 2007
1. Ren Fail. 2007;29(5):649-51. Relationship between insulin resistance and malondialdehyde level... more 1. Ren Fail. 2007;29(5):649-51. Relationship between insulin resistance and malondialdehyde levels in dialysis patients. Taskapan MC, Keskin L, Sahin I, Ozyalin F, Atmaca H, Temel I, Taskapan H. PMID: 17654332 [PubMed - indexed for MEDLINE]. Publication Types: Letter. ...
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