Papers by Fariba Sharififar
Veterinary Research Forum, Nov 27, 2011
The objective of this study was to investigate whether or not the adverse effects of heat on sper... more The objective of this study was to investigate whether or not the adverse effects of heat on sperm quality could be prevented by green tea extract (GTE) administration. Ninety adult male mice were randomly divided to two groups. The scrotum of each animal in the first group was immersed once for 20 min in a water bath maintained at 42 o C (heat group, H) and the second group (control group, C) was maintained at 23 o C. Heat-treated and control groups were allocated randomly into three subgroups. The first subgroup from heat-treated mice was administered sterile saline (heat saline, HS) and the two other subgroups were administered orally with two different doses of GTE including 500 and 750 mg kg-1 (HG500 and HG750) for 49 consecutive days. Likewise, the first subgroup from control mice was administered sterile saline (control saline, CS) and the two other subgroups were administered orally with 500 and 750 mg kg-1 of GTE (CG500 and CG750), respectively. Heat stress significantly reduced (P < 0.05) sperm concentration, total sperm motility, progressive sperm motility and hypo-osmotic swelling-water test positive spermatozoa at the first 14 days after the heat treatment. However, a partial recovery was observed at the day 42, which was still significantly lower than that of the CS group. Administration of GTE in two doses (HG500 and HG750 treatment groups) following heat treatment could significantly recover adverse effects of heat on above-mentioned parameters during the first 28 days. The present study demonstrates that the adverse effects of hyperthermia on semen parameters might be recovered following administration of green tea extract in a short period of time.
Journal of Integrative Medicine, 2020
Effects of a Plantago ovata-based herbal compound in prevention and treatment of oral mucositis i... more Effects of a Plantago ovata-based herbal compound in prevention and treatment of oral mucositis in patients with breast cancer receiving chemotherapy: A double-blind, randomized, controlled crossover trial,
Measurement, 2016
The interest in finding natural antioxidants for use in food, drugs or cosmetic products has incr... more The interest in finding natural antioxidants for use in food, drugs or cosmetic products has increased concerning the replacement of synthetic antioxidants due to their possible undesirable toxic effects on humans long-term exposed. Ethanolic extracts of Hypericum perforatum (Hypericaceae), Lavandula angustifolia (Lamiaceae), Malva sylvestris (Malvaceae), Melissa officinalis (Lamiaceae), Salvia officinalis (Lamiaceae), Rosmarinus officinalis (Lamiaceae) were obtained under sonication with a yield of 5.9%, 3.8%, 5.8%, 3.8%, 4.2% and 2.3% (w/w), respectively and their antioxidant activity assessed by the TLC-DPPH and DPPH assays. With exception of L. angustifolia and M. sylvestris extracts all the others showed antioxidant activity greater than 70% (at 0.1 mg/mL). The HPLC analysis shows that all tested extracts contain rosmarinic acid and suggest that H. perforatum extract have the highest concentration (2.76 mM) and M. sylvestris extract the lowest one (0.47 mM). In this study, we describe a simple laboratory procedure using an ultrasound-assisted ethanolic extraction from six medicinal plants. This potential didactic approach showed to be a rapid and effective process on preserving the antioxidant activity and rosmarinic acid 2 content. The HPLC system was used to evaluate the ability to assess rosmarinic acid content.
Iranian Journal of Toxicology, 2020
Background: The safety of the use of medicinal plants is a general challenge among consumers. To ... more Background: The safety of the use of medicinal plants is a general challenge among consumers. To improve the use, it is necessary to provide complete profiles of the natural medications for quality control and the therapeutic and toxicity effects. This study was conducted to evaluate the structural and functional toxicity of the methanolic extracts of Salvia rhytidea and Glycine max plants in mice. Methods: After determining the LD50, NMRI mice with mean weight of 25-30 g were treated intraperitoneally with 100, 200 and 400 mg/kg, and orally with 800 mg/kg of the extracts for 7 consecutive days. After the last treatment, the serum samples were prepared and used for the biochemical assays. The liver and left kidney were removed from the animals and fixed in 10% formalin for histopathological examinations. Results: The results indicated that the biochemical parameters of liver and kidneys were not significantly different among the experimental and control groups (P>0.05). Mild de...
Annales Pharmaceutiques Françaises
BMC Complementary Medicine and Therapies
Background The aim of the present study was to assess the expression and serum level of AMH in fi... more Background The aim of the present study was to assess the expression and serum level of AMH in first-generation female mice pups following fennel and flaxseed consumption. Methods Twenty pregnant NMRI mice were allocated into four groups including control (CTL), fennel (FV), flaxseed (LU) and FV+ LU. Sixty-four female offsprings after lactation period, received the same regimen as their mothers for 56 and 240 days. The ovarian follicles development, serum concentration of AMH, as well as gene and protein expression of AMH were evaluated in the female offsprings at post-natal day 56 (PND56) and 240 (PND240). Results The number of total growing follicles were raised in the FV group in compression to the all experimental groups. In contrast, LU group showed a marked decrease in their numbers. The highest level of serum AMH was seen in the FV-diet mice, whereas LU negatively affected it. The expression level of AMH also increased in the FV and FV + LU groups, while a reduction was obser...
Iranian Red Crescent Medical Journal
Background: Vulvovaginal candidiasis (VVC) is a very common debility in gynecology clinics. Despi... more Background: Vulvovaginal candidiasis (VVC) is a very common debility in gynecology clinics. Despite access to several antifungal agents, VVC is still a challenge; therefore, presenting a novel antifungal agent, especially with a traditional origin, is of interest and demand. Objectives: The current study aimed at comparing the efficacy of Anethum graveolens L. (dill) vaginal cream with that of 1% Clotrimazole vaginal cream to treat VVC. Methods: A prospective, single-center, randomized, double-blind clinical trial was performed. In the current study, married females aged 18-65 years, with probable vulvovaginal candidiasis were enrolled for primary evaluation. After VVC confirmation by the sniff test, the patients were allocated into three groups with regard to blocked randomization, and inclusion and exclusion criteria. The first group (n = 59) was treated with 1% Clotrimazole vaginal cream; the second (n = 60) with a combination of dill seed essential oil and dried aqueous extract vaginal cream (TEE); and the last (n = 56) with dill seed essential oil vaginal cream (EO) for seven nights. After 10 days, the therapeutic effects were assessed. Results: After the intervention, itching decreased to 30.5%, 3.3%, and 12.5% in the Clotrimazole, TEE, and EO groups, respectively; the sniff test also decreased to 33.9%, 13.3%, and 12.5%, respectively. Although a statistically significant difference, according to itching and sniff test, was observed among the three groups (P < 0.001, P = 0.005), there was no significant difference between TEE and EO (P = 0.06). Conclusions: The current study showed that the herbal preparation could be used as an alternative antifungal agent for vulvovaginal Candidiasis.
Journal of Medicinal Plants Research, Oct 16, 2011
The user has requested enhancement of the downloaded file. All in-text references underlined in b... more The user has requested enhancement of the downloaded file. All in-text references underlined in blue are added to the original document and are linked to publications on ResearchGate, letting you access and read them immediately.
Abstract: Background & Aims: Postprandial hyperglycemia results in the development of chronic com... more Abstract: Background & Aims: Postprandial hyperglycemia results in the development of chronic complications. Therefore, its control is critical in the treatment of diabetes. One of the main therapeutic approaches for reducing postprandial hyperglycemia is to reduce the absorption of carbohydrates by enzyme inhibitors such as Acarbose which have already been clinically used as a medicine for diabetic patients. Methods: Aqueous and methanolic extracts of Zataria multiflora, Pistachio hulls, Myrtle leaves and Quercus infectoria which had inhibited alpha glucosidase (>90%) in our previous study were prepared by maceration method. Male wistar rats were divided into two, streptozotocin-induced diabetic group and non-diabetic group. Then each group subdivided into 6 groups of control receiving 2g/kg maltose, positive control receiving 2g/kg maltose as well as Acarbose and 4 other groups receiving 2g/kg maltose and one of the prepared extracts (1000mg/kg) after deprivation of food. Blood...
Iranian Journal of Pharmaceutical Research : IJPR, 2018
Traditionally, Myrtus communis (myrtle) has been used for treatment of several kinds of disorders... more Traditionally, Myrtus communis (myrtle) has been used for treatment of several kinds of disorders. However, there are some factors, namely, low solubility and permeability, which restrict use of myrtle extract (ME) in medical applications. Regarding these limitations, the aim of the present study was to develop a new niosomal formulation to enhance ME stability and permeability. Briefly, several niosomal formulations were prepared by non-ionic surfactants and cholesterol with different molar ratios. Afterward, size, entrapment efficiency (EE%), release and stability of niosomal myrtle extract (nME) were investigated. The effect of ME and nME on viability of 3T3 cells was evaluated using MTT assay. Antibacterial activity of ME and nME was also assessed against Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli, Micrococcus luteus, and Bacillus subtilis. Sizes of niosomes were 5.3 ± 0.3 to 15.9 ± 2.2 µm with 4.1 ± 0.3 to 26.9 ± 1.7 mV zeta potential. The EE% of niosom...
Journal of Medicine and Life, 2015
Background: The analgesic paracetamol causes a potentially fatal, centrilobular hepatic necrosis ... more Background: The analgesic paracetamol causes a potentially fatal, centrilobular hepatic necrosis when taken in misuse and overdose. This research aimed to evaluate the protective effects of methanolic extract of Zataria Multiflora Boiss (Z. Multiflora) against hepatic damage induced by paracetamol-induced hepatotoxicity in male Wistar rats. Methods: for this purpose, paracetamol was administrated orally at a dose of 2 g/ kg body weight (b.w.)/ day on the seventh day after the oral administration of a methanolic extract of Z. Multiflora at doses of 100 mg/ kg, 200 mg/ kg and 400 mg/ kg b.w. The lipid peroxidation level and activities of liver aminotransferases and enzymes contributing to the oxidative damage were measured in serum, and a histopathological examination of liver sections was also performed. Results and Discussion: The results showed that Z. Multiflora reduced the activity of aminotransferases in rats treated with paracetamol. This extract also inhibited lipid peroxidati...
International Journal of Fertility & Sterility, 2019
Background Busulfan (BU) has a destructive effect on the male reproductive system. The goal of th... more Background Busulfan (BU) has a destructive effect on the male reproductive system. The goal of this study was to assess the effects of olive leaf extract (OLE) as a source of antioxidants and phenolic compounds, on BU-induced damages in rat testes. Materials and Methods In this experimental study, 40 male Wistar rats were randomly divided into 5 groups. The control group (CTL) received a single intraperitoneal (i.p.) injection of dimethyl sulfoxide (DMSO), followed by oral administration of distilled water for 5 weeks. In BU group, BU (10 mg/kg) was administrated i.p. once. In co- treatment groups, first, received BU (10 mg/kg, a single i.p. injection) then, OLE was administrated orally at different doses of 250 mg/kg (BU+OLE 250), 500 mg/kg (BU+OLE 500) and 750 mg/kg (BU+OLE 750), for 5 weeks. Next, blood and sperm samples were collected. The left testis was removed to investigate testicular parameters and apop- tosis by using H&E and TUNEL staining, respectively. All data were ana...
Research Journal of Pharmacognosy, 2017
Background and objectivesNatural products are one of the major sources for investigations of nove... more Background and objectivesNatural products are one of the major sources for investigations of novel medicines. Zataria multiflora Boiss (ZM) has shown pharmacological activities especially in gastrointestinal tract; however, there are limited studies about its cytotoxicity effects. In this study, the effect of Zataria multiflora was examined on two colon cancer cell lines (SW-48 and HT-29).MethodsHydro-alcoholic extract of ZM and its fractions including chloroform, petroleum ether and methanol extract were prepared by warm maceration method. Different concentrations were prepared and examined on SW-48 and HT-29 cell lines using 2-(4, 5-dimethylthiazol-2-yl) 2, 5-diphenyltetrazolium bromide (MTT) assay.ResultsThe results of the present study have shown the cytotoxic effect of some fractions of ZM. The most considerable cytotoxic effect was shown against HT-29 cell line. Also, total ZM extract and the petroleum ether fraction demonstrated cytotoxic effects with IC50 values of 44.22 and...
Journal of Environmental Science and Health, Part B, 2021
Abstract Malathion (MT) is one of the most widely used organophosphorus insecticides which induce... more Abstract Malathion (MT) is one of the most widely used organophosphorus insecticides which induces toxicity through oxidative stress induction, free radical production and acetylcholinesterase inhibition. In this work, HepG2 cells were used to determine the effect of Zataria multiflora methanolic extract (MEZM) and rosmarinic acid (RA) on MT-induced cytotoxicity, oxidative stress, and apoptosis. Total phenolic content (TPC) and total flavonoid content (TFC) were determined and plant was further standardized based on RA content using HPLC method. The cultured HepG2 cells were pretreated with MEZM (1 μg/ml) and RA (0.1 μg/ml) for 4 h and exposed to MT (100 μM). Cell viability, oxidative stress biomarkers, ROS production, and cell death were examined after 24 h. The amount of RA was determined 73.48 mg/g dried extract. IC50 values of MEZM and MT were 368.56 μg/ml and 99.43 μM, respectively. Pretreatment with MEZM and RA decreased the cytotoxicity, oxidative stress, and cell percentage in the late apoptosis and necrosis stages induced by MT. There was no significant difference between MEZM and RA effects. The present study showed the significant protective effects of MEZM against toxicity induced by MT in hepatocytes which can be attributed to the plant antioxidant constituents including RA.
Nanomedicine, 2021
Aim: To develop a novel method for the bioanalytical extraction of trigonelline (TRG) from human ... more Aim: To develop a novel method for the bioanalytical extraction of trigonelline (TRG) from human plasma samples using a magnetic nanocomposite and to evaluate its pharmacokinetic profile. Materials & methods: Magnetic bentonite/β-cyclodextrine (β-CD) nanoparticles, coupled with a validated ion-pairing reversed-phase high-performance liquid chromatography method, were used to determine TRG concentration from plasma samples following a single oral administration. Results: The developed reversed-phase high-performance liquid chromatography method was accurate, precise, specific, selective and reproducible. TRG showed rapid absorption, middle rate of elimination and mean residence time of ∼24 h. The data were best fitted on a two-compartment model in which tmax was 1.0 h, Cmax 0.115 μg/ml, area under the curve (AUC)0–24 1.72 μg/ml.h, Cl 0.0293 l/h/kg, t1/2α 0.79 h, t1/2β 13.68 h and ka 1.63 h-1. Conclusion: The findings of this study could provide useful information to promote the futur...
Biointerface Research in Applied Chemistry, 2020
In neonatal jaundice, β-glucuronidase converts conjugated bilirubin into the unconjugated form an... more In neonatal jaundice, β-glucuronidase converts conjugated bilirubin into the unconjugated form and increases its level in the blood. Many natural compounds have been identified as β-glucuronidase inhibitors. The aim of this study was to evaluate the effect of hydro-methanolic extracts of 100 plants on β-glucuronidase. The β-glucuronidase activity was measured by a spectrophotometric method using Phenolphthalein glucuronide and 4-nitrophenyl β-D-glucuronide. Kinetic study of the enzyme was performed in the presence and absence of the plant extract. It was revealed that from hydro-methanolic (70%) extracts, Rosa damascena and Ipomoea tricolor showed more than 85% inhibitory effect on β-glucuronidase. Rosa damascena showed competitive inhibition, and Ipomoea tricolor showed non-competitive inhibition. The Km and Vmax values for β-glucuronidase were 23.32 mM and 0.814 mM min-1, respectively. When using 4-nitrophenyl β-D-glucuronide, Stevia and Cerasus avium showed more than 65% inhibito...
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Papers by Fariba Sharififar