Papers by Fabio Brambilla
BMC Veterinary Research, 2020
An amendment to this paper has been published and can be accessed via the original article.
BMC Veterinary Research, 2020
Background This study evaluated the effects of partial substitution of dietary fishmeal (FM) with... more Background This study evaluated the effects of partial substitution of dietary fishmeal (FM) with either fish protein hydrolysate (FPH) or autolysed dried yeast (HiCell®, Biorigin, Brazil) on intestinal microbiota of gilthead sea bream (Sparus aurata). A total number of 720 fish of 122.18 ± 6.22 g were fed for 92 days with three different diets in triplicate (3 tanks/diet). A diet based on FM/vegetable meal was used as control. The other two diets were formulated by replacing FM with 5% of either FPH or HiCell®. To analyze the gut microbiota associated to autochthonous and allochthonous microbial communities, the Illumina MiSeq platform for sequencing of 16S rRNA gene and QIIME pipeline were used. Results A total number of 102 OTUs (operational taxonomic units) at 97% identity were identified in fish gut samples collected at the end of feeding trial. Fourteen OTUs constituted the core gut microbiota, i.e. those OTUs found in at least nine out of fifteen samples per group and shared ...
The application and the development of marine biotic index AMBI was performed. The comparison bet... more The application and the development of marine biotic index AMBI was performed. The comparison between AMBI results and the ecological index calculated (Margalef index, Shannon-Wiener index, Simpson index and Pielou index) showed that the influence of the mariculture activities is localized and that the health status of the benthic community is not compromised. Introduzione -L'allevamento intensivo in gabbie produce una considerevole quantità di prodotti di rifiuto costituiti prevalentemente da mangime non ingerito, feci e prodotti di escrezione. Lo studio degli effetti di queste sostanze è stato oggetto di numerosi studi pubblicati negli ultimi anni (Carroll et al., 2003). Vari indici biotici sono stati proposti al fine di valutare l'incidenza delle attività antropiche sull'ecosistema bentonico in acque costiere (Muxika et al., 2005). Tra questi vi è l'AZTI Marine Biotic Index (AMBI), sviluppato da Borja et al. (2000), che permette una classificazione dei siti sulla ...
Aquaculture International, 2018
Context. Our knowledge of the intrinsic parameters of exoplanets is as precise as our determinati... more Context. Our knowledge of the intrinsic parameters of exoplanets is as precise as our determinations of their stellar hosts parameters. In the case of radial velocity searches for planets, stellar masses appear to be crucial. But before estimating stellar masses properly, detailed spectroscopic analysis is essential. With this paper we conclude a general spectroscopic description of the Pennsylvania-Toruń Planet Search (PTPS) sample of stars. Aims. We aim at a detailed description of basic parameters of stars representing the complete PTPS sample. We present atmospheric and physical parameters for dwarf stars observed within the PTPS along with updated physical parameters for the remaining stars from this sample after the first Gaia data release. Methods. We used high resolution (R=60 000) and high signal-to-noise-ratio (S/N=150-250) spectra from the Hobby-Eberly Telescope and its High Resolution Spectrograph. Stellar atmospheric parameters were determined through a strictly spectroscopic local thermodynamic equilibrium analysis (LTE) of the equivalent widths of Fe I and Fe II lines. Stellar masses, ages, and luminosities were estimated through a Bayesian analysis of theoretical isochrones. Results. We present T eff , log g, [Fe/H], micrturbulence velocities, absolute radial velocities, and rotational velocities for 156 stars from the dwarf sample of PTPS. For most of these stars these are the first determinations. We refine the definition of PTPS subsamples of stars (giants, subgiants, and dwarfs) and update the luminosity classes for all PTPS stars. Using available Gaia and Hipparcos parallaxes, we redetermine the stellar parameters (masses, radii, luminosities, and ages) for 451 PTPS stars. Conclusions. The complete PTPS sample of 885 stars is composed of 132 dwarfs, 238 subgiants, and 515 giants, of which the vast majority are of roughly solar mass; however, 114 have masses higher than 1.5 M and 30 of over 2 M . The PTPS extends toward much less metal abundant and much more distant stars than other planet search projects aimed at detecting planets around evolved stars; 29% of our targets belong to the Galactic thick disc and 2% belong to the halo.
Italian Journal of Animal Science, 2009
Saroglia (2009) Molecular characterization and in vivo expression of hypoxia inducible factor (HI... more Saroglia (2009) Molecular characterization and in vivo expression of hypoxia inducible factor (HIF)-1α in sea bass (Dicentrarchus labrax) exposed to acute and chronic hypoxia,
Current Issues in Molecular Biology
In aquafeeds in which plant proteins are used to replace fishmeal, exogenous methionine (Met) sou... more In aquafeeds in which plant proteins are used to replace fishmeal, exogenous methionine (Met) sources are demanded to balance the amino acid composition of diets and meet the metabolic fish requirements. Nonetheless, since different synthetic Met sources are commercially available, it is important to determine their bioavailability and efficacy. To address this issue, we conducted a two-month feeding trial with rainbow trout (Oncorhynchus mykiss), which were fed diets supplemented with five different forms of Met: Met-Met, L-Met, HMTBa, DL-Met, and Co DL-Met. No differences in growth performance were found in trout fed with different Met forms, but changes in the whole-body composition were found. In particular, Met-Met and L-Met promoted a significant body lipid reduction, whereas the protein retention was significantly increased in fish fed with HMTBa and Co DL-Met. The latter affected the hepatic Met metabolism promoting the trans-sulfuration pathway through the upregulation of C...
Animals
Microalgal oils (AOs) emerged recently as an alternative to fish oil and to nutritionally poorer ... more Microalgal oils (AOs) emerged recently as an alternative to fish oil and to nutritionally poorer vegetable oils for fish species. In this trial, two experimental diets containing fish oil (negative control: 2.1%; positive control: 13.8%) and two diets incorporating AO at 3.5 and 0.7% were fed to grow out gilthead seabream (Sparus aurata) of 64.5 g initial body weight. After 110 days of experimental feeding, performance (final body weight mean = 147 g) and survival (>99%) were similar across treatments. The highest eicosapentaenoic acid (EPA) + docosahexaenoic acid (DHA) content in positive control (PC) and 3.5 AO feeds (3.11 and 2.18% of diet, respectively) resulted in the highest EPA + DHA deposition in the fillets (18.40 and 12.36 g/100 g fatty acid, respectively), which entirely reflected the dietary fatty acid profile. Feed and fillets from fish fed the AO diets had lower levels of dioxins and polychlorinated biphenyls (PCBs). Moreover, sensory quality of AO fillets scored eq...
Frontiers in Marine Science
The present research tested the effects of dietary nisin-producing Lactococcus lactis on growth p... more The present research tested the effects of dietary nisin-producing Lactococcus lactis on growth performance, feed utilization, intestinal morphology, transcriptional response, and microbiota in gilthead sea bream (Sparus aurata). A feeding trial was conducted with fish weighting 70–90 g. Fish were tagged with passive, integrated transponders and distributed in nine 500 L tanks with 40 fish each. Fish were fed for 12 weeks with either a control (diet A) or experimental diets (diets B and C) in triplicate (3 tanks/diet). Extruded pellets of diets B and C were supplemented with a low (2 × 109 CFU/kg) and a high (5 × 109 CFU/kg) dose of probiotic, respectively. No significant differences were found between groups for the feed conversion ratio or specific growth rates. However, the final body weight of fish fed diet C was significantly higher than the control group with intermediate values for fish fed diet B. Histological analysis conducted using a semi-quantitative scoring system showe...
Italian Journal of Animal Science
Miragliotta (2019): Effect of hydrolysed fish protein and autolysed yeast as alternative nitrogen... more Miragliotta (2019): Effect of hydrolysed fish protein and autolysed yeast as alternative nitrogen sources on gilthead sea bream (Sparusaurata) growth performances and gut morphology,
PloS one, 2018
Animal by-product meals from the rendering industry could provide a sustainable and commercially ... more Animal by-product meals from the rendering industry could provide a sustainable and commercially viable alternative to fishmeal (FM) in aquaculture, as they are rich in most essential amino acids and contain important amounts of water-soluble proteins that improve feed digestibility and palatability. Among them, poultry by-product meal (PBM) have given encouraging results in rainbow trout (Oncorhynchus mykiss). However, the introduction of new ingredients in the diet needs to be carefully evaluated since diet is one of the main factors affecting the gut microbiota, which is a complex community that contributes to host metabolism, nutrition, growth, and disease resistance. Accordingly, we investigated the effects of partial replacement of dietary FM with a mix of animal by-product meals and plant proteins on intestinal microbiota composition of rainbow trout in relation to growth and feeding efficiency parameters. We used 1540 trout with an initial mean body weight of 94.6 ± 14.2 g. ...
Journal of Experimental Marine Biology and Ecology, Jul 1, 2009
Italian Journal of Animal Science, 2010
Marine fish farming generates particulate wastes which are dispersed in the sea environment. To d... more Marine fish farming generates particulate wastes which are dispersed in the sea environment. To deal with this problem, particulate waste dispersion models have been developed to predict the effects of fish cage culture. In this study, we evaluated the seabed deposition of a fish farming facility located in the central western Mediterranean by using the Meramod ® model. The objectives where first to assess the actual scenario, and second to forecast the possible impact due to the forthcoming enlargement of the farming area with the addition of new fish cages. By computing the hydrodynamic measurements and the daily amount of feed recorded between July and December 2006, the impact seabed surfaces forecasted by the model increased from 5.6ha in the actual scenario, up to 7.3ha in the future. The model estimated a maximum level of total solid flux deposition of 3,800g/m 2 bed/year and a maximum level of total carbon flux deposition of 1,350g/m 2 bed/year for both scenarios. Furthermore, the model predicted that the installation of 4 new fish cages (with an hypothetical mean daily amount of feed of 50kg/cage) will produce a total solid and carbon flux deposition levels ranging 0-400 and 0-150g/m 2 bed/year respectively, under the new fish cages location.
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Papers by Fabio Brambilla