Papers by FRANCISCO GONZALEZ DEL PINO
Frontiers in Veterinary Science, Jan 5, 2021
To provide new insights into the mechanisms through which seminal plasma proteins can protect spe... more To provide new insights into the mechanisms through which seminal plasma proteins can protect sperm from damage caused during refrigeration, we evaluate the possibility that β-NGF can contribute to the improvement of sperm quality after cooling. First, β-NGF was detected in refrigerated sperm and compared with unrefrigerated sperm by western blotting of the proteins adsorbed by sperm, showing that native β-NGF is still present even 24 h after cooling only as an active form. Then, the effect of exogenous β-NGF on sperm quality after cooling was evaluated. A total of 12 ejaculates from male llamas (three ejaculates per male), were obtained by electro-ejaculation, diluted 4:1 with buffer Hepes-balanced salt solution and centrifuged at 800 × g for 8 min to remove the seminal plasma. Sperm were suspended in Tris-citrate-fructose-egg yolk diluent for a final concentration of 30 × 10 6 /ml and cooled at 5 • C for 24 h. After refrigeration, the extended sperm were equilibrated for 5 min at 37 • C and divided into the following subgroups: sperm samples without treatment (control) and sperm samples supplemented with exogenous human β-NGF (10, 100, and 500 ng/ml). At 5, 30, and 60 min of incubation sperm were evaluated for sperm viability (using eosin/nigrosin stain), sperm motility and vigor (observed under light microscopy), and mitochondrial activity (using the JC-1 fluorescent marker). Vigor data were analyzed with the nonparametric Kruskal-Wallis test. The rest of the variables were analyzed with a mixed models approach. Mean comparisons were performed using Fisher's LSD test with a confidence level of 95%. A principal components analysis was performed to analyze the relationships between variables. Treatment of 24 h cooled sperm with 10 or 100 ng/ml of human β-NGF increased the percentage of total motility and vigor (p < 0.05). Besides, an incubation time of 60 min would be adequate to improve sperm quality, since all variables are positively related. The significant improvement observed in the motility and vigor of post-refrigerated sperm suggests that supplementation with exogenous β-NGF may be profitable for the improvement of cooled llama sperm.
Redvet Revista Electronica De Veterinaria, 2009
El imprinting es un proceso de formación de la personalidad del recién nacido que ocurre en un br... more El imprinting es un proceso de formación de la personalidad del recién nacido que ocurre en un breve y sensible período que sigue al alumbramiento, de carácter irreversible. Su comprensión y manejo exige la aplicación de una técnica o de un arte, por el cual el humano se introduce en un animal lactante inmediatamente luego de su nacimiento. El principio de aprendizaje en el que se basan las técnicas de imprinting es el de habituación.
Redvet Revista Electronica De Veterinaria, 2013
Los objetivos de este artículo son presentar una breve revisión bibliográfica sobre esta neoplasi... more Los objetivos de este artículo son presentar una breve revisión bibliográfica sobre esta neoplasia y describir un caso con un cuadro clínico de un de un tumor de células de la Granulosa TCG en una yegua de raza Peruano de Paso de 4 años de edad. La misma fue diagnosticada en forma presuntiva por los signos clínicos que presentaba el animal, tales como comportamiento sexual anormal y similar un padrillo, reflejo de Flehmen, monta de otras yeguas en celo, agresividad. El tumor fue confirmado mediante ecografía, método sencillo y confiable para el veterinario que trabaja a campo.
Redvet Revista Electronica De Veterinaria, 2009
es una de las endocrinopatías más comunes en esta especie. Se trata de una condición progresiva y... more es una de las endocrinopatías más comunes en esta especie. Se trata de una condición progresiva y lenta, debida a la presencia de un adenoma, hiperplasia o hipertrofia a nivel de la pars intermedia de la Pituitaria.
Redvet Revista Electronica De Veterinaria, 2010
El artículo describe el caso clínico de un cuadro de aborto en un yegua de raza Peruano de Paso d... more El artículo describe el caso clínico de un cuadro de aborto en un yegua de raza Peruano de Paso de 10 años de edad, debido a una preñez de mellizos con fijación bicornual. La misma fue diagnosticada en forma precoz mediante ecografía transrectal, pero su desenlace final en un aborto indeseable se debió a que el propietario del animal, no quiso su resolución mediante la técnica de reducción manual de uno de los mellizos.
Redvet Revista Electronica De Veterinaria, 2009
El artículo describe el caso clínico de una piometra en un equino hembra de raza Peruano de Paso ... more El artículo describe el caso clínico de una piometra en un equino hembra de raza Peruano de Paso de 19 años de edad, diagnosticado mediante ecografía y su resolución mediante la técnica de lavaje uterino, utilizando una sonda tipo Foley. Método simple y práctico para el veterinario que trabaja a campo.
Redvet Revista Electronica De Veterinaria, 2011
To provide new insights into the mechanisms through which seminal plasma proteins can protect spe... more To provide new insights into the mechanisms through which seminal plasma proteins can protect sperm from damage caused during refrigeration, we evaluate the possibility that β-NGF can contribute to the improvement of sperm quality after cooling. First, β-NGF was detected in refrigerated sperm and compared with unrefrigerated sperm by western blotting of the proteins adsorbed by sperm, showing that native β-NGF is still present even 24 h after cooling only as an active form. Then, the effect of exogenous β-NGF on sperm quality after cooling was evaluated. A total of 12 ejaculates from male llamas (three ejaculates per male), were obtained by electro-ejaculation, diluted 4:1 with buffer Hepes-balanced salt solution and centrifuged at 800 × g for 8 min to remove the seminal plasma. Sperm were suspended in Tris-citrate-fructose-egg yolk diluent for a final concentration of 30 ×106/ml and cooled at 5°C for 24 h. After refrigeration, the extended sperm were equilibrated for 5 min at 37°C ...
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Papers by FRANCISCO GONZALEZ DEL PINO