In addition to its role in genome protection, DNA methylation can regulate gene expression. In th... more In addition to its role in genome protection, DNA methylation can regulate gene expression. In this study, we characterized the impact of acidity, phase variation, and the ArsRS TCS on the expression of the Type I m6A DNA methyltransferase HsdM1 (HP0463) ofHelicobacter pylori26695 and their subsequent effects on the methylome. Transcription ofhsdM1 increases at least 4-fold in the absence of the sensory histidine kinase ArsS, the major acid-sensing protein ofH. pylori.hsdM1 exists in the phase-variable operonhsdR1-hsdM1. Phase-lockinghsdR1 (HP0464), the restriction endonuclease gene, has significant impacts on the transcription ofhsdM1. To determine the impacts of methyltransferase transcription patterns on the methylome, we conducted methylome sequencing on samples cultured at pH 7 or pH 5. We found differentially methylated motifs between these growth conditions, and that deletions ofarsSand/orhsdM1 interfere with the epigenetic acid response. Deletion ofarsSleads to altered activ...
In addition to its role in genome protection, DNA methylation can regulate gene expression. In th... more In addition to its role in genome protection, DNA methylation can regulate gene expression. In this study, we characterized the impact of acidity, phase variation, and the ArsRS TCS on the expression of the Type I m6A DNA methyltransferase HsdM1 (HP0463) ofHelicobacter pylori26695 and their subsequent effects on the methylome. Transcription ofhsdM1 increases at least 4-fold in the absence of the sensory histidine kinase ArsS, the major acid-sensing protein ofH. pylori.hsdM1 exists in the phase-variable operonhsdR1-hsdM1. Phase-lockinghsdR1 (HP0464), the restriction endonuclease gene, has significant impacts on the transcription ofhsdM1. To determine the impacts of methyltransferase transcription patterns on the methylome, we conducted methylome sequencing on samples cultured at pH 7 or pH 5. We found differentially methylated motifs between these growth conditions, and that deletions ofarsSand/orhsdM1 interfere with the epigenetic acid response. Deletion ofarsSleads to altered activ...
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Papers by Erin Ramsey