Papers by Eloi Dezan Junior
Brazilian Oral Research
Marina Tolomei Sandoval CURY (a) Ana Maria Veiga VASQUES (a) Gustavo SIVIERI-ARAÚJO (a) Rogério C... more Marina Tolomei Sandoval CURY (a) Ana Maria Veiga VASQUES (a) Gustavo SIVIERI-ARAÚJO (a) Rogério Castilho JACINTO (a) João Eduardo GOMES-FILHO (a) Luciano Tavares Angelo CINTRA (a) Eloi DEZAN-JÚNIOR (a)
Brazilian oral research, Jan 24, 2018
Based on aroeira's (Myracrodruon urundeuva) antimicrobial activity and a future trend to comp... more Based on aroeira's (Myracrodruon urundeuva) antimicrobial activity and a future trend to compose intracanal medication, the aim of this study was to assess in vivo inflamatory tissue response to the extracts by edemogenic and histological analysis containing inactivated facultative and anaerobic microorganisms. For edema quantification, eighteen animals were divided into three groups (n = 3, periods: 3 and 6 hours) and 0.2 mL of 1% Evans blue per 100 g of body weight was injected into the penile vein under general anesthesia. After 30 min the animals received a subcutaneous injection in the dorsal region of aqueous or ethanolic extract of aroeira or saline (control) containing inactivated bacteria. Samples were collected, immersed in formamide for 72h, and evaluated by spectrophotometry (630 m). For histological analysis, polyethylene tubes with the extracts were implanted in the dorsal of 30 male rats. Analysis of the fibrous capsule and inflammatory infiltrate were performed a...
Clinical oral investigations, Jan 9, 2018
Evaluate, in vitro, the antimicrobial activity of Psidium cattleianum leaf extracts combined with... more Evaluate, in vitro, the antimicrobial activity of Psidium cattleianum leaf extracts combined with calcium hydroxide against Enterococcus faecalis and Candida albicans biofilm. Dentin specimens obtained from extracted bovine incisors were infected during 14 days with E. faecalis ATCC 29212 and C. albicans ATCC 10231. The specimens were filled with calcium hydroxide pastes prepared with the following vehicles: Psidium cattleianum ethanolic, Psidium cattleianum propylene glycolic, distilled water, and saline as control. After 24 h, 3, 7, and 14 days, the canals were irrigated with sterile saline and dried. Dentin samples were collected from the canals with burs of increasing diameters. To determine the number of colony-forming units (CFU), samples were inoculated onto BHI agar supplemented with yeast extract (0.5%), at 37 °C, for 48 h, in COenriched atmosphere. Comparisons among the groups for the variation factors were performed by ANOVA and Tukey's test. Ethanolic and propylene g...
Brazilian oral research, Jan 3, 2017
The aim of this study was to evaluate edemogenic activity and subcutaneous inflammatory reaction ... more The aim of this study was to evaluate edemogenic activity and subcutaneous inflammatory reaction induced by Psidium cattleianum leaf extracts associated with Ca(OH)2. Thirty male Wistar rats, split equally into three groups [aqueous extract + Ca(OH)2; ethanolic extract + Ca(OH)2; and propylene glycol + Ca(OH)2], were assessed every 3 h or 6 h (five animals in each period). Under general anesthesia, 0.2 mL of 1% Evans blue per 100 g of body weight was injected into the penile vein and each combination to be evaluated was subcutaneously injected into the dorsal region 30 min thereafter. Edemogenic activity was analyzed by spectrophotometry (λ=630 nm). For inflammatory reaction analysis, 50 rats received four polyethylene tubes (three experimental groups) and an empty tube (control group). The assessments were made at 7, 15, 30, 60, and 90 days, followed by hematoxylin-eosin staining and by the assignment of scores for evaluation of tissue response intensity. Ethanolic extract + Ca(OH)...
Trabalho experimental 4.2 Microrganismo 4.2.1 Obtenção do microrganismo 4.2.2 Inoculação nos anim... more Trabalho experimental 4.2 Microrganismo 4.2.1 Obtenção do microrganismo 4.2.2 Inoculação nos animais experimentais 4.3 Remoção das peças teciduais 4.4 Recuperação do microrganismo inoculado 4.5 Confirmação da identidade do microrganismo recuperado 4.6 Preparo para análise microscópica 4.6.1 Fixação 4.6.2 Desmineralização 4.6.3 Inclusão e microtomia 4.6.4 Coloração 4.7 Análise microscópica 5 RESULTADOS 5.1 Análise microbiológica da recuperação do microrganismo isolado 5.2 Análise microscópica 5.2.1 Observações relativas à morfologia 5.2.2 Observações relativas à presença e localização das bactérias 5.3 Observações relativas à presença e localização de Leishmania sp. 6 DISCUSSÃO 6.1 Da concepção 6.2 Da metodologia 6.2.1 Do tratamento endodôntico 6.2.2 Emprego de microrganismo com marcador 6.2.3 Processamento laboratorial das peças 6.3 Dos resultados 6.3.1 Implantação de microrganismo exógeno 6.3.2 Microrganismos diferentes que apareceram nos espécimes de 30 dias após bacteremia 6.3.3 Os porquês de não aparecer os microrganismos 6.3.4 Dos achados microscópicos
Objective: The aim of the study was to evaluate the influence of periapical lesions and/or period... more Objective: The aim of the study was to evaluate the influence of periapical lesions and/or periodontal disease on the long-term glycemic control in a rat model of diabetes mellitus. Method: Eighty male Wistarrats were divided into eight groups of ten animals each: normal rats (G1), rats with pulpal disease (G2), rats with periodontal disease (G3), rats with pulpal and periodontal diseases (G4), diabetic rats (G5), diabetic rats with pulpal disease (G6), diabetic rats with periodontal disease (G7), diabetic rats with periodontal and pulpal diseases (G8). Diabetes was induced using streptozotocin, periapical lesions were induced by dental pulp exposure to the oral environment and the periodontal disease by periodontal ligature. The animals were sacrificed after 30 days and the glycosylated hemoglobin levels (Hb1Ac) were measured with ion exchange chromatography. The total assessed values was tabulated according to each experimental group and statistically analyzed by analysis of varia...
Objective: The aim of this study was to evaluatethe influence of partial mixingof sealers and its... more Objective: The aim of this study was to evaluatethe influence of partial mixingof sealers and its correlation to the biological compatibility through immediate reaction (edema) and histological evaluation. Method: Wistar rats (n=40) were divided into 4 groups and under general anesthesia received intravenous injection of Evans blue. After 30min, the subcutaneous was injected with 0.1ml of Sealapex® or AH Plus® with complete or partial mixing. After 3 (n=20) and 6 hours (n=20) rats were euthanatized, the pieces were removed, macerated and placed in formamide for 72 hours and analyzed by absorbance spectrophotometry. For the histological evaluation (n=20), each wistar rat received four polyethylene tube implants in the dorsum, filled with endodontic sealers partially or totally mixed. Post operative periods were 7 (n=10) and 30 days (n=10). Samples were analyzed by light microscopy after glycol methacrylate embedding, 3-mm serial cutting, and hematoxylin/eosin stain. Quantitative eval...
Objective: Plant extracts of "araçá" (Psidium cattleianum) are biocompatible and inhibi... more Objective: Plant extracts of "araçá" (Psidium cattleianum) are biocompatible and inhibit oral microorganisms. Association of these extracts to calcium hydroxide (Ca(OH)2) inhibited E. faecalis after 24 hours while Ca(OH)2 associated with propylene glycol or distilled water needed 7 to 14 days for the same result. The aim of this study was to evaluate the biocompatibility of Ca(OH)2 associated with "araçá" extracts in different vehicles through immediate reaction (edema) and histological evaluation. Method: Immediate Reaction test: Wistar rats (n=48) were divided into 4 groups (n=12) and under general anesthesia received intravenous injection of Evans blue. After 30min, the subcutaneous was injected with 0.1ml of Ca(OH)2 mixed with distilled water, Ca(OH)2 + aqueous extract, Ca(OH)2 + ethanolic extract, or Ca(OH)2 + ethanolic extract resuspended in propylene glycol. Rats were euthanatized after 3 (n=20) and 6 hours (n=20). The tissue was extracted, macerated and p...
Objective: The aim of this study was to evaluate the effects of periapical lesions and/or periodo... more Objective: The aim of this study was to evaluate the effects of periapical lesions and/or periodontal disease on mean platelet count in a rat model of diabetes mellitus. Method: Eighty male Wistar rats were divided into eight groups of ten animals each: normal rats (G1), rats with pulpal disease (G2), rats with periodontal disease (G3), rats with pulpal and periodontal diseases (G4), diabetic rats (G5), diabetic rats with pulpal disease (G6), diabetic rats with periodontal disease (G7), diabetic rats with periodontal and pulpal diseases (G8). Diabetes was induced using streptozotocin, the periapical lesions were induced by dental pulp exposure to the oral environment and the periodontal disease by periodontal ligature. After 30 days, blood was collected by cardiac puncture and the animals were killed. The mean platelet counts measurement was carried out on an automatic blood counter, within one hour after sampling to avoid swelling of platelets. The results were analyzed by analysis...
Quintessence international (Berlin, Germany : 1985)
To analyze the clinical behavior of 2 pit and fissure sealants through clinical/computerized eval... more To analyze the clinical behavior of 2 pit and fissure sealants through clinical/computerized evaluation. Occlusal sealants were placed on maxillary and mandibular first and second premolars according to a split-mouth design. All premolars were sealed with either Concise (3M Espe) or Prisma Shield (Caulk/Dentsply) sealant agents. A hematoxylin-based staining solution was applied on the occlusal surface 7 days, 18 months, 36 months, and 11 years after occlusal sealing to allow checking of the sealant material on the surface. At each analysis time all occlusal surfaces were photographed, and the photographs corresponding to each time period were analyzed with SigmaScan 4.0 Software. The alterations of the sealed area of each sealant were analyzed with the software and recorded. Next, the measurements of the areas were tabulated and analyzed according to each period. Analysis of variance (ANOVA), with parts subdivided into time, and the t test, with a significance level of 5%, were used...
Journal of endodontics, 2001
This study was conducted to observe the healing process of intentional lateral root perforation r... more This study was conducted to observe the healing process of intentional lateral root perforation repaired with mineral trioxide aggregate (MTA). Forty-eight root canals of dogs' teeth were instrumented and filled. After partial removal of the filling, an intentional perforation was made with a bur in the lateral area of the root. The perforations were repaired with MTA or Sealapex (control group). Histological analysis occurred 30 and 180 days after treatment. Results showed no inflammation and deposition of cementum over MTA in the majority of the specimens. In the 180-day period, Sealapex exhibited chronic inflammation in all the specimens and slight deposition of cementum over the material in only three cases. In conclusion, MTA exhibited better results than the control group.
Brazilian dental journal, 1998
The subject of this paper was to study the behavior of the periapical tissues of dogs' teeth ... more The subject of this paper was to study the behavior of the periapical tissues of dogs' teeth after biopulpectomy and dressing with calcium hydroxide or a corticosteroid-antibiotic association, before root canal filling with zinc oxide eugenol (ZOE) or Sealapex sealers. The teeth were overinstrumented and dressed for 7 days before the root canal filling. The animals were sacrificed 180 days after treatment and the specimens were prepared for morphological analysis. Specimens treated with Sealapex presented a higher number of cases with biological closure than ZOE. When the root canals were filled with ZOE, better results were observed with the use of the Ca(OH)2 dressing.
Acta de odontología pediátrica, 1981
Journal of Endodontics, 1996
This study was an in vitro analysis of the apical seal of root canals filled with an experimental... more This study was an in vitro analysis of the apical seal of root canals filled with an experimental calcium hydroxide gutta-percha point. One hundred and ten recently extracted human permanent teeth with a single root were used. The root canals were biomechanically prepared, and some received a dressing of calcium hydroxide for 7 days. Root canals not dressed were filled by the lateral condensation technique with either regular gutta-percha or calcium hydroxide gutta-percha points. The dressed root canals were filled with the same sealers and regular gutta-percha points. The specimens were placed into a 2% methylene blue dye solution under vacuum, and the apical leakage was linearly evaluated. The experimental groups with calcium hydroxide dressing and the one with calcium hydroxide gutta-percha points exhibited significantly (p < 0.05) less leakage than the groups filled with regular gutta-percha points. The results obtained indicated that calcium hydroxide gutta-percha points produced an improvement in the apical sealing quality of the root canal filling.
Brazilian dental journal, 2002
The purpose of this paper was to study the reaction of rat subcutaneous connective tissue to the ... more The purpose of this paper was to study the reaction of rat subcutaneous connective tissue to the implantation of dentin tubes filled with white mineral trioxide aggregate (MTA), a material that will be marketed. The tubes were implanted into rat subcutaneous tissue and the animals were sacrificed after 7 and 30 days. The undecalcified pieces were prepared for histological analysis with polarized light and von Kossa technique for mineralized tissues. Granulations birefringent to polarized light and an irregular structure like a bridge were observed next to the material; both were von Kossa positive. Also, in the dentin wall tubules a layer of birefringent granulations was observed. The results were similar to those reported for gray MTA, indicating that the mechanisms of action of the white and gray MTA are similar.
Journal of endodontics, 2002
This study was conducted to observe the rat subcutaneous connective tissue reaction to implanted ... more This study was conducted to observe the rat subcutaneous connective tissue reaction to implanted dentin tubes that were filled with mineral trioxide aggregate, Sealapex, Calciobiotic Root Canal Sealer (CRCS), Sealer 26, and the experimental material, Sealer Plus. The animals were sacrificed after 7 and 30 days, and the specimens were prepared for histological analysis after serial sections with a hard-tissue microtome. The undecalcified sections were examined with polarized light after staining according to the Von Kossa technique for calcium. At the tube openings, there were Von Kossa-positive granules that were birefringent to polarized light. Next to these granulations, there was irregular tissue, like a bridge, that was Von Kossa-positive. The dentin walls of the tubes exhibited a structure highly birefringent to polarized light, usually like a layer, in the tubules. These results were observed with all the studied materials, except the CRCS, which didn't exhibit any kind of...
To evaluate the influence of diabetes mellitus on the tissue response to mineral trioxide aggrega... more To evaluate the influence of diabetes mellitus on the tissue response to mineral trioxide aggregate (MTA) and its ability to stimulate mineralization. Twenty-four male Wistar rats were divided into a non-diabetic control group and another with Alloxan-induced diabetes. Two polyethylene tubes, one kept empty as a control and the other containing Angelus MTA(®) , were implanted into the dorsal connective tissue of all rats for 30 days. Animals in each group received injections of calcein, alizarin, and oxytetracycline on day 7, 14, and 21, respectively. Animals were killed after 30 days; specimens were prepared by staining with hematoxylin and eosin (HE) and von Kossa technique as well as for examination of unstained sections with polarized light and fluorescence microscopy. The inflammatory reaction to the implanted tubes was equally mild in both groups. Structures staining with von Kossa were seen in response to Angelus MTA(®) , as were birefringent structures visualized on polarized light analysis; these had no relation to diabetic condition (P < 0.05). Fluorescence intensity was not changed in diabetic rats either (P < 0.05). Diabetes mellitus did not influence the tissue response to Angelus MTA(®) or the mineralization stimulated by it.
Journal of Endodontics, 2007
It has been shown that the mineral trioxide aggregate (MTA) used to seal lateral/furcal perforati... more It has been shown that the mineral trioxide aggregate (MTA) used to seal lateral/furcal perforations stimulates the deposition of newly formed cementum. Nevertheless, when the site of the perforation is contaminated, the healing process might occur under less favorable conditions. This study evaluated the repair healing process of noncontaminated and contaminated lateral perforations filled with MTA and the effect of previously filling the contaminated perforations with a bactericidal agent. Thirty lateral root perforations were prepared in endodontically treated dog's teeth, thus forming 3 groups with 10 specimens each. In group 1 the perforations were immediately sealed with MTA. In group 2 the perforations were left open for 7 days and thereafter sealed with MTA. In group 3 the perforations were left open for 7 days, filled temporarily with a calcium hydroxide-based paste for 14 days, and then sealed with MTA. The animals were killed after 90 days, and the pieces were prepared for histomorphologic and histomicrobiologic evaluations. The statistical analysis showed that group 1 had significantly better repair than groups 2 (P Ͻ .05) and 3 (P Ͻ .05), which validates the superior results obtained when MTA was immediately used to seal root perforations. Groups 2 and 3 had statistically similar repair to each other (P Ͼ .05). There were a larger number of cases of complete or partial biologic seal in group 1 compared with the contaminated groups. It might be concluded that the lateral root perforations sealed with MTA after contamination presented worse repair than the noncontaminated, immediately sealed perforations. The temporary filling with a bactericidal agent (calcium hydroxide-based paste) did not improve the repair of perforations exposed to contamination, and the contaminated groups presented similar results to each other.
Journal of endodontics, 2008
This study evaluated the histomorphologic response of human dental pulps capped with mineral trio... more This study evaluated the histomorphologic response of human dental pulps capped with mineral trioxide aggregate (MTA) and Ca(OH) 2 cement (CH). Pulp exposures were performed on the occlusal floor of 40 human permanent premolars. After that, the pulp was capped either with CH or MTA and restored with composite resin. After 30 and 60 days, teeth were extracted and processed for histologic exam and categorized in a histologic score system. The data were subjected to Kruskal-Wallis and Conover tests (␣ ϭ .05). All groups performed well in terms of hard tissue bridge formation, inflammatory response, and other pulpal findings. However, a lower response of CH30 was observed for the dentin bridge formation, when compared with MTA30 and MTA60 groups. Although the pulp healing with calcium hydroxide was slower than that of MTA, both materials were successful for pulp capping in human teeth.
Journal of Applied Oral Science, 2013
S ome manufacturers have recently added specific components to improve the ease of handling and i... more S ome manufacturers have recently added specific components to improve the ease of handling and insertion material properties of MTA in order to create MTA-based sealers. Objective: The aim of this study was to evaluate the healing of periapical lesions in canine teeth after a single session of endodontic treatment with MTA Fillapex ® compared with Sealapex ® or endo-CPM-Sealer ®. Material and Methods: Sixty-two root canals were performed on two 1-year-old male dogs. After coronal access and pulp extirpation, the canals were exposed to the oral cavity for 6 months in order to induce periapical lesions. The root canals were prepared, irrigated with a solution of 2.5% sodium hypochlorite and filled with gutta-percha and different sealers, according to the following groups: 1) Sealapex ® ; 2) endo-CPM-Sealer ® ; and 3) MTA Fillapex ®. Some teeth with periapical lesions were left untreated for use as positive controls. Healthy teeth were used as negative controls. After 6 months, the animals were sacrificed and serial sections from the roots were prepared for histomorphologic analysis and stained with hematoxylin and eosin and the Brown and Brenn technique. The lesions were scored according to pre-established histomorphologic parameters and the scores statistically analyzed using the Kruskal-Wallis test. Results: All 3 materials produced similar patterns of healing (p>0.05); in particular, persistent inflammation and absence of complete periapical tissue healing were consistently noted. Conclusions: Preparation of the infected root canals followed by filling with the materials studied was insufficient to provide complete healing of the periapical tissues.
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Papers by Eloi Dezan Junior