Papers by Edilene Batista
Food Chemistry, 2004
Total phenols and condensed tannins in Pigeon pea seeds were determined by flow injection spectro... more Total phenols and condensed tannins in Pigeon pea seeds were determined by flow injection spectrophotometry. Folin-Denis and vanillin assays were used for total phenol and condensed tannin, respectively. Three Pigeon pea accessions were cultivated in a greenhouse. After harvesting, one access was separated into two aliquots. One aliquot was freeze-dried ()196°C), and the other was dried in a forced air oven at 50°C, like the other access samples, to evaluate drying temperature interference. The seeds were initially analyzed and then stored at around )10°C, 16°C or at ambient temperature (25 AE 10°C). The stored samples were analysed every 30 days, for a 90 day period. A significant difference ðP < 0:05Þ occurred between the drying methods for condensed tannin as well as for total phenols, regardless of the storage conditions. The experiments for comparing total phenols, obtained at different storage times, showed significant differences ðP < 0:05Þ for total phenols, indicating that tannin quantification has to be done immediately after harvesting to avoid formation of protein complexes or polymerisation, which lead to erroneous results. In accordance with results, freeze-drying proved to be best for preserving the characteristics of the sample. In order to compare the tannin and phenol contents within accessions, fresh seeds should be used.
Journal of Invertebrate Pathology, 2009
... Recently, a group of scientists representing 34 institutions around the world published a dra... more ... Recently, a group of scientists representing 34 institutions around the world published a draft genome of one of the varieties, the black cottonwood tree Populus trichocarpa, as an interesting model forest species for comparative plant genomics (Tuskan et al., 2006). ...
Journal of The Brazilian Chemical Society, 2003
ABSTRACT A flow system, coupled with flame atomic absorption spectrometry (FIA-FAAS), was develop... more ABSTRACT A flow system, coupled with flame atomic absorption spectrometry (FIA-FAAS), was developed for tannin determination in pigeon pea samples, exploring the precipitation reaction between tannins and proteins. Sample extracts obtained by sonication with a 50% (v/v) methanol solution were introduced into the system and induced to react with a hemoglobin solution. The precipitate produced was retained on a filter located in the analytical flow. A reversed flow of 1% (w/v) sodium dodecyl sulfate solution was used for solubilization of the precipitate from the filter and to conduct the tannin-hemoglobin complex to the FAAS, to quantify the iron ions present in the hemoglobin structure. A tannic acid solution was used to prepare the analytical curve. The proposed method allowed determination of 30 samples per hour, a standard deviation of 9.7% (n=10), and a quantification limit of 0.27 mg L-1 for tannic acid.
Dedico este projeto primeiramente a Deus e em seguida a Bruno, Fernanda, Fabio, Fabiana e Fernand... more Dedico este projeto primeiramente a Deus e em seguida a Bruno, Fernanda, Fabio, Fabiana e Fernanda (filhos e noras), com carinho, estes que são fonte de minha inspiração.
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Papers by Edilene Batista