Saltar a navegación, búsqueda Iglesia de Sant Climent en Taüll, Lérida Se llama estilo románico e... more Saltar a navegación, búsqueda Iglesia de Sant Climent en Taüll, Lérida Se llama estilo románico en arquitectura al resultado de la combinación razonada y armónica de elementos constructivos y ornamentales de procedencia latina, oriental (bizantinos, sirios, persas y árabes) y septentrional (celtas, germánicos, normandos) que se formó en la Europa cristiana durante los primeros siglos de la baja Edad Media.
Background Cryptosporidiosis represents a major public health problem. This infection has been re... more Background Cryptosporidiosis represents a major public health problem. This infection has been reported worldwide as a frequent cause of diarrhoea. Particularly, it remains a clinically significant opportunistic infection among immunocompromised patients, causing potentially life-threatening diarrhoea in HIV-infected persons. However, the understanding about different aspects of this infection such as invasion, transmission and pathogenesis is problematic. Additionally, it has been difficult to find suitable animal models for propagation of this parasite. Efforts are needed to develop reproducible animal models allowing both the routine passage of different species and approaching unclear aspects of Cryptosporidium infection, especially in the pathophysiology field. Results We developed a model using adult severe combined immunodeficiency (SCID) mice inoculated with Cryptosporidium parvum or Cryptosporidium muris while treated or not with Dexamethasone (Dex) in order to investigate divergences in prepatent period, oocyst shedding or clinical and histopathological manifestations. C. muris-infected mice showed high levels of oocysts excretion, whatever the chemical immunosuppression status. Pre-patent periods were 11 days and 9.7 days in average in Dex treated and untreated mice, respectively. Parasite infection was restricted to the stomach, and had a clear preferential colonization for fundic area in both groups. Among C. parvum-infected mice, Dex-treated SCID mice became chronic shedders with a prepatent period of 6.2 days in average. C. parvum-inoculated mice treated with Dex developed glandular cystic polyps with areas of intraepithelial neoplasia, and also with the presence of intramucosal adenocarcinoma. Conclusion For the first time C. parvum is associated with the formation of polyps and adenocarcinoma lesions in the gut of Dex-treated SCID mice. Additionally, we have developed a model to compare chronic muris and parvum cryptosporidiosis using SCID mice treated with corticoids. This reproducible model has facilitated the evaluation of clinical signs, oocyst shedding, location of the infection, pathogenicity, and histopathological changes in the gastrointestinal tract, indicating divergent effects of Dex according to Cryptosporidium species causing infection.
Brazilian Archives of Biology and Technology, 2005
The objective of this work was to determine the fecundity of the predator Brontocoris tabidus (Si... more The objective of this work was to determine the fecundity of the predator Brontocoris tabidus (Signoret) (Heteroptera: Pentatomidae) females of two weight classes aiming to define, which one presented higher productivity in the laboratory. Males and females of B. tabidus were reared from nymphs fed with Tenebrio molitor L. (Coleoptera: Tenebrionidae) pupae in laboratory. Females of B. tabidus weighting 95 to 150 mg and those with 160 to 220 mg constituted two treatments. Oviposition period and numbers of egg masses, eggs and nymphs per female of B. tabidus were higher in the treatment with heavier females, while the periods of preoviposition, between egg mass laying, egg incubation and number of eggs per egg mass, besides the percentage of nymphs hatched and adult longevity were similar between treatments. Heavier females of B. tabidus presented better productivity and for this reason they should be used in programs of mass rearing this predator.
We examined 364 school children for intestinal parasites in a sub-urban zone of Caracas, Venezuel... more We examined 364 school children for intestinal parasites in a sub-urban zone of Caracas, Venezuela. Giardia intestinalis was the most prevalent parasite in stool samples from 34 children. Levels of IgA and IgG antibodies to G. intestinalis were assessed by enzyme-linked immunosorbent assay and Western blot before and after treatment with secnidazole. All patients were cured with a reduction of IgA antibody levels in 26 of 34 children and a reduction in IgGspecific antibody levels in 18 of 34 children. Serum of infected patients reacted with proteins of 14 kD to 137 kD. Some patients did not show a change in IgA serum reactivity for parasite proteins by Western blot after treatment. Seventeen children showed reduction of the reactivity or disappearance of protein reactivity (mainly the 14-kD, 122-kD, and 137-kD proteins). Antibody response was not related to clinical status, but quantitative and qualitative serum antibody response against G. intestinalis infection could be used to assess levels of new protein markers that decrease or disappear with successful chemotherapy.
A scheme for temperature control of a greenhouse is presented. The present work proposes an appro... more A scheme for temperature control of a greenhouse is presented. The present work proposes an approach based on a combination of two different control schemes: Feedback Linearization (FL) and standard linear Model Predictive Control (MPC), using their advantages. The treated greenhouse is considered a non-linear Single-Input–Single-Output process and subject to strong external disturbances. Since the methodology used for solving the MPC + FL approaches generally leads to an optimization problem subject to state-dependent non-linear constraints, an alternative for its implementation is discussed. Two control techniques are compared, namely MPC + FL and Non-linear Model Predictive Control (NLMPC).
Saltar a navegación, búsqueda Iglesia de Sant Climent en Taüll, Lérida Se llama estilo románico e... more Saltar a navegación, búsqueda Iglesia de Sant Climent en Taüll, Lérida Se llama estilo románico en arquitectura al resultado de la combinación razonada y armónica de elementos constructivos y ornamentales de procedencia latina, oriental (bizantinos, sirios, persas y árabes) y septentrional (celtas, germánicos, normandos) que se formó en la Europa cristiana durante los primeros siglos de la baja Edad Media.
Background Cryptosporidiosis represents a major public health problem. This infection has been re... more Background Cryptosporidiosis represents a major public health problem. This infection has been reported worldwide as a frequent cause of diarrhoea. Particularly, it remains a clinically significant opportunistic infection among immunocompromised patients, causing potentially life-threatening diarrhoea in HIV-infected persons. However, the understanding about different aspects of this infection such as invasion, transmission and pathogenesis is problematic. Additionally, it has been difficult to find suitable animal models for propagation of this parasite. Efforts are needed to develop reproducible animal models allowing both the routine passage of different species and approaching unclear aspects of Cryptosporidium infection, especially in the pathophysiology field. Results We developed a model using adult severe combined immunodeficiency (SCID) mice inoculated with Cryptosporidium parvum or Cryptosporidium muris while treated or not with Dexamethasone (Dex) in order to investigate divergences in prepatent period, oocyst shedding or clinical and histopathological manifestations. C. muris-infected mice showed high levels of oocysts excretion, whatever the chemical immunosuppression status. Pre-patent periods were 11 days and 9.7 days in average in Dex treated and untreated mice, respectively. Parasite infection was restricted to the stomach, and had a clear preferential colonization for fundic area in both groups. Among C. parvum-infected mice, Dex-treated SCID mice became chronic shedders with a prepatent period of 6.2 days in average. C. parvum-inoculated mice treated with Dex developed glandular cystic polyps with areas of intraepithelial neoplasia, and also with the presence of intramucosal adenocarcinoma. Conclusion For the first time C. parvum is associated with the formation of polyps and adenocarcinoma lesions in the gut of Dex-treated SCID mice. Additionally, we have developed a model to compare chronic muris and parvum cryptosporidiosis using SCID mice treated with corticoids. This reproducible model has facilitated the evaluation of clinical signs, oocyst shedding, location of the infection, pathogenicity, and histopathological changes in the gastrointestinal tract, indicating divergent effects of Dex according to Cryptosporidium species causing infection.
Brazilian Archives of Biology and Technology, 2005
The objective of this work was to determine the fecundity of the predator Brontocoris tabidus (Si... more The objective of this work was to determine the fecundity of the predator Brontocoris tabidus (Signoret) (Heteroptera: Pentatomidae) females of two weight classes aiming to define, which one presented higher productivity in the laboratory. Males and females of B. tabidus were reared from nymphs fed with Tenebrio molitor L. (Coleoptera: Tenebrionidae) pupae in laboratory. Females of B. tabidus weighting 95 to 150 mg and those with 160 to 220 mg constituted two treatments. Oviposition period and numbers of egg masses, eggs and nymphs per female of B. tabidus were higher in the treatment with heavier females, while the periods of preoviposition, between egg mass laying, egg incubation and number of eggs per egg mass, besides the percentage of nymphs hatched and adult longevity were similar between treatments. Heavier females of B. tabidus presented better productivity and for this reason they should be used in programs of mass rearing this predator.
We examined 364 school children for intestinal parasites in a sub-urban zone of Caracas, Venezuel... more We examined 364 school children for intestinal parasites in a sub-urban zone of Caracas, Venezuela. Giardia intestinalis was the most prevalent parasite in stool samples from 34 children. Levels of IgA and IgG antibodies to G. intestinalis were assessed by enzyme-linked immunosorbent assay and Western blot before and after treatment with secnidazole. All patients were cured with a reduction of IgA antibody levels in 26 of 34 children and a reduction in IgGspecific antibody levels in 18 of 34 children. Serum of infected patients reacted with proteins of 14 kD to 137 kD. Some patients did not show a change in IgA serum reactivity for parasite proteins by Western blot after treatment. Seventeen children showed reduction of the reactivity or disappearance of protein reactivity (mainly the 14-kD, 122-kD, and 137-kD proteins). Antibody response was not related to clinical status, but quantitative and qualitative serum antibody response against G. intestinalis infection could be used to assess levels of new protein markers that decrease or disappear with successful chemotherapy.
A scheme for temperature control of a greenhouse is presented. The present work proposes an appro... more A scheme for temperature control of a greenhouse is presented. The present work proposes an approach based on a combination of two different control schemes: Feedback Linearization (FL) and standard linear Model Predictive Control (MPC), using their advantages. The treated greenhouse is considered a non-linear Single-Input–Single-Output process and subject to strong external disturbances. Since the methodology used for solving the MPC + FL approaches generally leads to an optimization problem subject to state-dependent non-linear constraints, an alternative for its implementation is discussed. Two control techniques are compared, namely MPC + FL and Non-linear Model Predictive Control (NLMPC).
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Papers by Eduardo Piñon