Criado para tratar os temas que limitam o acesso de fazendeiros em pequena escala, na África sub-... more Criado para tratar os temas que limitam o acesso de fazendeiros em pequena escala, na África sub-Saara, para que possuam recursos para obterem colheitas de sementes de qualidade, das quais eles dependem para a obtenção dos alimentos e é o seu próprio meio de vida. Esta coleção de artigos descreve os princípios bem sucedidos para as experiências em comunidades que possuem produções de sementes. Entre outras coisas, os textos analisarão o sistema de produção normal de sementes e seus modelos, proporão caminhos para criar esquemas bem sucedidos para as comunidades que produzam sementes, descreverão as práticas próprias da produção de sementes para cereais seletos, plantas vegetativamente propagadas e outras colheitas, e esquematizarão as práticas básicas de negócios para os produtores de sementes.
Peanut is an important and nutritious agricultural commodity and a livelihood of many small-holde... more Peanut is an important and nutritious agricultural commodity and a livelihood of many small-holder farmers in the semi-arid tropics (SAT) of world which are facing serious production threats. Integration of genomics tools with on-going genetic improvement approaches is expected to facilitate accelerated development of improved cultivars. Therefore, high-resolution genotyping and multiple season phenotyping data for 50 important agronomic, disease and quality traits were generated on the ‘reference set ’ of peanut. This study reports comprehensive analyses of allelic diversity, population structure, linkage disequilibrium (LD) decay and marker-trait association (MTA) in peanut. Distinctness of all the genotypes
Intermittent drought is the most important yield limiting factor affecting groundnut (Arachis hyp... more Intermittent drought is the most important yield limiting factor affecting groundnut (Arachis hypogaea L.) production in rain-fed regions of Sub-Saharan Africa and Asia. Improvement of crop adaptation to drought is needed and this starts by having a thorough assessment of a large and representative set of germplasm. In this study, 247 lines belonging to the reference collection of groundnut were assessed under wellwatered (WW) and intermittent water stress (WS) conditions in India and Niger for two years, following similar experimental protocols. The WS treatment reduced pod yield (31-46%), haulm yield (8-55%) and the harvest index (1-10%). Besides a strong treatment effect, yield differences within locations and years, were attributed to both genotypic and genotype-by-treatment interactions. Pod yield under WW and WS conditions were closely related in both years (Patancheru, r 2 = 0.42 and r 2 = 0.50; Sadore, r 2 = 0.22 and r 2 = 0.23). By contrast, within location and treatment, pod and haulm yields were affected predominantly by genotype-by-year (G × Y) effects, especially under WS. Within treatment across locations and years, pod and haulm yields were mostly ruled by genotypic effects, which allowed identifying a group of entries with contrasting pod yield across locations under WS. However, genotype and genotype by environment (GGE) biplot analyses distinguished India from Niger, suggesting that the selection remains environment-specific and also revealed dissimilarity between years in Niger. A close relationship was observed between yield and pod growth rate (r 2 = 0.51), and partition (r 2 = 0.33) under WS conditions, whereas no significant relationship was found between yield under WS and SCMR, or specific leaf area (SLA). These results showing a close interaction between the environmental conditions and the genotypic response to intermittent drought shows the necessity to carefully choose environments that truly represent target environments. This is an important result in the current breeding context of marker-assisted recurrent selection or genome-wide selection. This work opens also new ways for the breeding of drought tolerant groundnut, by bringing new highly contrasting lines currently used for crossing and deciphering drought adaptation traits to better understand G × E interactions, while it challenges the relevance of long-time used surrogates such as SCMR or SLA.
The Bill and Melinda Gates Foundation (BMGF) funded Tropical Legumes (TL III) project was impleme... more The Bill and Melinda Gates Foundation (BMGF) funded Tropical Legumes (TL III) project was implemented in seven sub-Saharan Africa countries (Burkina Faso, Ghana, Mali, Nigeria, Ethiopia, Tanzania, and Uganda) and South Asia (India). Shortage of seed of improved varieties has been identified as the greatest hindrance to farmer adoption of new agricultural technologies developed through this project. This chapter compares the different approaches followed by different countries in the establishment of Multi-Stakeholder Platforms (MSPs) for supply of improved legume seed to farmers. Achievements from this initiative are mixed and multi-dimensional. The details herein provide the reader with insights on the level of success of innovation platforms in the different countries and implications for agricultural technology dissemination to smallholder farmers. Key achievements include strengthened linkages among various legume seed value chain actors, participation of several cadres of seed ...
Designed to address the issues that limit the access of small-scale farmers in sub-Saharan Africa... more Designed to address the issues that limit the access of small-scale farmers in sub-Saharan Africa to quality, affordable seed of the crops on which they depend for food security and livelihoods, this collection of articles describes successful principles for and experiences in community-based seed production. Among other things, the manuscripts analyze current seed production systems and models; propose ways to design successful community-based seed production schemes; describe proper seed production practices for selected cereals, vegetatively propagated plants, and other crops; and outline basic business practices for seed producers.
The study aimed to quantify the immediate effects of dietary diversification, food safety, and hy... more The study aimed to quantify the immediate effects of dietary diversification, food safety, and hygiene interventions on child undernutrition in four rural villages in Kongwa district of central Tanzania. One hundred mothers with their children of less than 24 months old were recruited for this study. The difference-indifference (DID) method was used to assess the effects of intensive intervention through a learning-by-doing process on the topic of aflatoxin free diversified food utilization and improved hygiene practices. Periodic anthropometric measurements were conducted on the 0th, 7th, 14th, and 21st days, and DID estimator showed the significant and positive average marginal effects of the intervention on Z-Scores being 0.459, 0.252, and 0.493 for wasting, stunting, and underweight, respectively. Notably, at the end of the study, the mean aflatoxin M 1 level in urine samples decreased by 64% in the intervention group, while it decreased by 11% in the control group. The study provides quantitative evidence on intensive 21-day training for mothers incorporating integrated technologies yielded positive impacts on their children's nutritional outcomes.
This article highlights 12 years (2007-2019) of research, achievements, lessons learned, challeng... more This article highlights 12 years (2007-2019) of research, achievements, lessons learned, challenges and gaps in discovery-to-delivery research in legumes emanating from three projects, collectively called Tropical Legumes (TL) with a total investment of about US$ 67 million funded by the Bill & Melinda Gates Foundation. These projects were implemented by three CGIAR centres (ICRISAT, CIAT and IITA) together with 15 national agricultural research system partners in sub-Saharan Africa and South Asia. The TL projects together with some of their precursors and complementary projects from other agencies, facilitated the development of 266 improved legume varieties and the production of about 497,901 tons of certified seeds of the target legume crops in the focus countries. The certified seeds have been planted on about 5.0 million ha by more than 25 million smallholder farmers in the 15 countries and beyond, producing about 6.1 million tons of grain worth US$ 3.2 billion. Furthermore, the projects also trained 52 next generation scientists that included 10 women, by supporting 34 Masters degrees and 18 PhD degrees. 1 | INTRODUC TI ON The Tropical Legumes (TL) projects supported by the Bill & Melinda Gates Foundation (BMGF) were jointly implemented by three CGIAR Centres (ICRISAT, CIAT and IITA) together with 15 national agricultural research system (NARS) partners in select focus countries
There are several hurdles to ensure sustainable seed production and consistent flow of improved l... more There are several hurdles to ensure sustainable seed production and consistent flow of improved legume varieties in sub-Saharan Africa (SSA) and South Asia (SA). The unreliable demand, autogamous nature of most of the grain legumes, and slow variety replacement rate by smallholder farmers do not provide strong incentive for private seed companies to invest in legume seed business. Unless a well thought-out and comprehensive approach to legume seed delivery is developed, current seed shortages will continue, eroding emerging market opportunities. The experiences reported here are collated through a 10-year partnership project, the Tropical Legumes in SSA and SA. It fostered innovative public-private partnerships in joint testing of innovative market-led seed systems, skills and knowledge enhancement, de-risking private sector initiatives that introduced in new approaches and previously overlooked entities in technology delivery. As new public and private seed companies, individual seed entrepreneurs and farmer organizations emerged, the existing ones enhanced their capacities. This resulted in significant rise in production, availability and accessibility of various seed grades of newly improved and farmer demanded legume varieties in the target countries. K E Y W O R D S decentralized seed production and supply, improved legume variety innovative legume seed systems, multi-stakeholder platform, sub-Saharan Africa and South Asia, variety promotion How to cite this article: Rubyogo J-C, Akpo E, Omoigui L, et al. Market-led options to scale up legume seeds in developing countries: Experiences from the Tropical Legumes Project.
Groundnut is an important food and oil crop in the semiarid tropics, contributing to household fo... more Groundnut is an important food and oil crop in the semiarid tropics, contributing to household food consumption and cash income. In Asia and Africa, yields are low attributed to various production constraints. This review paper highlights advances in genetics, genomics and breeding to improve the productivity of groundnut. Genetic studies concerning inheritance, genetic variability and heritability, combining ability and trait correlations have provided a better understanding of the crop's genetics to develop appropriate breeding strategies for target traits. Several improved lines and sources of variability have been identified or developed for various economically important traits through conventional breeding. Significant advances have also been made in groundnut genomics including genome sequencing, marker development and genetic and trait mapping. These advances have led to a better understanding of the groundnut genome, discovery of genes/variants for traits of interest and integration of marker-assisted breeding for selected traits. The integration of genomic tools into the breeding process accompanied with increased precision of yield trialing and phenotyping will increase the efficiency and enhance the genetic gain for release of improved groundnut varieties.
Legumes are important components of sustainable agricultural production, food, nutrition and inco... more Legumes are important components of sustainable agricultural production, food, nutrition and income systems of developing countries. In spite of their importance, legume crop production is challenged by a number of biotic (diseases and pests) and abiotic stresses (heat, frost, drought and salinity), edaphic factors (associated with soil nutrient deficits) and policy issues (where less emphasis is put on legumes compared to priority starchy staples). Significant research and development works have been done in the past decade on important grain legumes through collaborative bilateral and multilateral projects as well as the CGIAR Research Program on Grain Legumes (CRP-GL). Through these initiatives, genomic resources and genomic tools such as draft genome sequence, resequencing data, large-scale genomewide markers, dense genetic maps, quantitative trait loci (QTLs) and diagnostic markers have been developed for further use in multiple genetic and breeding applications. Also, these mega-initiatives facilitated release of a number of new varieties and also dissemination of on-the-shelf varieties to the farmers. More efforts are needed to enhance genetic gains by reducing the time required in cultivar development through integration of genomics-assisted breeding approaches and rapid generation advancement.
Aflatoxin-lysine (AFB-lys) adduct levels in blood samples collected from 230 individuals living i... more Aflatoxin-lysine (AFB-lys) adduct levels in blood samples collected from 230 individuals living in three districts of Malawi (Kasungu, Mchinji, and Nkhotakota) and aflatoxin B (AFB) levels in groundnut and maize samples collected from their respective homesteads were determined using indirect competitive enzyme-linked immunosorbent assay (IC-ELISA) methods. AFB-lys adducts were detected in 67% of blood samples, with a mean concentration of 20.5 ± 23.4 pg/mg of albumin. AFB was detected in 91% of groundnut samples and in 70% of maize samples, with mean AFB levels of 52.4 and 16.3 μg/kg, respectively. All participants of this study reported consuming maize on a daily basis and consuming groundnuts regularly (mean consumption frequency per week: 3.2 ± 1.7). According to regression analysis, a frequency of groundnut consumption of more than four times per week, being female, and being a farmer were significant (p < 0.05) contributors to elevated AFB-lys adduct levels in the blood. Th...
Th is study was conducted to determine the interaction between chickpea genotypes with the enviro... more Th is study was conducted to determine the interaction between chickpea genotypes with the environment (GxE) on the yield stability and adaptability of desi type chickpea genotypes (Cicer arietinum L.). Seventeen chickpea genotypes were evaluated for two cropping years (2012/2013-2013/2014) at four locations i.e., eight environments (locations x years combination). Chickpea grain yield was significantly (p<0.01) affected by genotypes, the environments and GxE interaction, indicating that the varieties and the test environments were diverse. GxE was further partitioned by principal component axes. The first two principal components cumulatively explained 53.1% of the total variation, of which 32.7% and 20.4% were contributed by IPCA1 and IPCA2, respectively. This implies that the interaction of 17 chickpea genotypes with eight environments was predicted by the first two principal components. AMMI1 biplot analysis showed five adaptive categories of genotypes based on similarities in their performance across environments. The AMMI2 biplot generated using genotypes and environmental scores for the first two IPCAs revealed positioning of the five genotype groups (GC) into four sectors of the biplot. Among them, two genotypes in GC 5 (G5 and G11) exhibited high yields across environments, low IPCA1 scores, low AMMI stability value (ASV) and yield stability index (YSI). G5 was released as a new variety, 'Dimtu' and registered in the Official Varieties Catalogue of Ethiopia, 2016.
In Zambia, groundnut products (milled groundnut powder, groundnut kernels) are mostly sold in und... more In Zambia, groundnut products (milled groundnut powder, groundnut kernels) are mostly sold in under-regulated markets. Coupled with the lack of quality enforcement in such markets, consumers may be at risk to aflatoxin exposure. However, the level of aflatoxin contamination in these products is not known. Compared to groundnut kernels, milled groundnut powder obscures visual indicators of aflatoxin contamination in groundnuts such as moldiness, discoloration, insect damage or kernel damage. A survey was therefore conducted from 2012 to 2014, to estimate and compare aflatoxin levels in these products (n = 202), purchased from markets in important groundnut growing districts and in urban areas. Samples of whole groundnut kernels (n = 163) and milled groundnut powder (n = 39) were analysed for aflatoxin B 1 (AFB 1) by competitive enzyme-linked immunosorbent assay (cELISA). Results showed substantial AFB 1 contamination levels in both types of groundnut products with maximum AFB 1 levels of 11,100 μg/kg (groundnut kernels) and 3000 μg/kg (milled groundnut powder). However, paired t test analysis showed that AFB 1 contamination levels in milled groundnut powder were not always significantly higher (P > 0.05) than those in groundnut kernels. Even for products from the same vendor, AFB 1 levels were not consistently higher in milled groundnut powder than in whole groundnut kernels. This suggests that vendors do not systematically sort out whole groundnut kernels of visually poor quality for milling. However, the overall contamination levels of groundnut products with AFB 1 were found to be alarmingly high in all years and locations. Therefore, solutions are needed to reduce aflatoxin levels in such under-regulated markets.
It is not known which aflatoxigenic species are present in Zambia. Therefore, soil samples were c... more It is not known which aflatoxigenic species are present in Zambia. Therefore, soil samples were collected during May to June 2012, at the end of the growing season in Eastern Province, from 399 farmers' fields that had been planted to groundnut (Arachis hypogaea L.) in Nyimba, Petauke, Mambwe, and Chipata Districts. Population densities of Aspergillus spp. were estimated by plating 10−3 soil dilutions on modified dichloran rose Bengal (MDRB) media. To test for toxigenicity, colonies were randomly selected from MDRB dilution plates, single-spored, transferred to vials with yeast extract sucrose (YES) liquid media, and grown for a week at room temperature. Agra Strip ® lateral flow cards were then used to test the filtered extracts, from the YES cultures, for total aflatoxin at 4 and 20 parts per billion (ppb). We identified Aspergillus flavus (small and large sclerotia strains), A. parasiticus, A. niger, A. nomius, A. oryzae, A. tamarii, and A. terreus. 100% of the S-strain A. fl...
A 3-year comprehensive analysis of aflatoxin contamination in peanut butter was conducted in Zamb... more A 3-year comprehensive analysis of aflatoxin contamination in peanut butter was conducted in Zambia, sub-Saharan Africa. The study analyzed 954 containers of 24 local and imported peanut butter brands collected from shops in Chipata, Mambwe, Petauke, Katete, and Nyimba districts and also in Lusaka from 2012 to 2014. For analysis, a sample included six containers of a single brand, from the same processing batch number and the same shop. Each container was quantitatively analyzed for aflatoxin B1 (AFB1) in six replicates by using competitive enzyme-linked immunosorbent assay; thus, aflatoxin contamination level of a given sample was derived from an average of 36 test values. Results showed that 73% of the brands tested in 2012 were contaminated with AFB1 levels >20 μg/kg and ranged up to 130 μg/kg. In 2013, 80% of the brands were contaminated with AFB1 levels >20 μg/kg and ranged up to 10,740 μg/kg. Compared with brand data from 2012 and 2013, fewer brands in 2014, i.e., 53%, h...
Background Throughout the world, and particularly in Africa, budgets for national agricultural re... more Background Throughout the world, and particularly in Africa, budgets for national agricultural research systems (NARS) are suffering severe cuts. In this context, it is impossible for programs to research all crop and livestock enterprises across all agroecologies. Nor is it possible to pursue a full range of strategic, applied and adaptive research for each priority problem.
Rising food and nutritional insecurity threatens the livelihoods of millions of poor people, part... more Rising food and nutritional insecurity threatens the livelihoods of millions of poor people, particularly in sub-Saharan Africa. Vegetable and legume production and consumption are a potent mechanism for small-scale, disadvantaged farmers to obtain the required nutrients in their diets and to generate much-needed income through trade. Vegetables and legumes are key sources of nutrients and health-promoting phytochemicals, providing higher micronutrient contents and a wider spectrum of essential compounds to meet nutritional and health needs than other food sources. Diversifying diets with vegetables and legumes is a cheaper, surer, and more sustainable way to supply a range of nutrients to the body and combat malnutrition and associated health problems than other approaches that target only a single or a few nutritional factors. Furthermore, vegetables and legumes often accompany staple crops in meals, and most staple crops are less palatable without vegetable or legume accompaniments. As a growing world population demands more and higher quality foods, and as environmental problems such as soil degradation, water scarcity, biodiversity loss, and climate change become more acute, the need for innovative vegetable and legume research solutions to improve food and nutritional security cannot be overemphasized.
Criado para tratar os temas que limitam o acesso de fazendeiros em pequena escala, na África sub-... more Criado para tratar os temas que limitam o acesso de fazendeiros em pequena escala, na África sub-Saara, para que possuam recursos para obterem colheitas de sementes de qualidade, das quais eles dependem para a obtenção dos alimentos e é o seu próprio meio de vida. Esta coleção de artigos descreve os princípios bem sucedidos para as experiências em comunidades que possuem produções de sementes. Entre outras coisas, os textos analisarão o sistema de produção normal de sementes e seus modelos, proporão caminhos para criar esquemas bem sucedidos para as comunidades que produzam sementes, descreverão as práticas próprias da produção de sementes para cereais seletos, plantas vegetativamente propagadas e outras colheitas, e esquematizarão as práticas básicas de negócios para os produtores de sementes.
Peanut is an important and nutritious agricultural commodity and a livelihood of many small-holde... more Peanut is an important and nutritious agricultural commodity and a livelihood of many small-holder farmers in the semi-arid tropics (SAT) of world which are facing serious production threats. Integration of genomics tools with on-going genetic improvement approaches is expected to facilitate accelerated development of improved cultivars. Therefore, high-resolution genotyping and multiple season phenotyping data for 50 important agronomic, disease and quality traits were generated on the ‘reference set ’ of peanut. This study reports comprehensive analyses of allelic diversity, population structure, linkage disequilibrium (LD) decay and marker-trait association (MTA) in peanut. Distinctness of all the genotypes
Intermittent drought is the most important yield limiting factor affecting groundnut (Arachis hyp... more Intermittent drought is the most important yield limiting factor affecting groundnut (Arachis hypogaea L.) production in rain-fed regions of Sub-Saharan Africa and Asia. Improvement of crop adaptation to drought is needed and this starts by having a thorough assessment of a large and representative set of germplasm. In this study, 247 lines belonging to the reference collection of groundnut were assessed under wellwatered (WW) and intermittent water stress (WS) conditions in India and Niger for two years, following similar experimental protocols. The WS treatment reduced pod yield (31-46%), haulm yield (8-55%) and the harvest index (1-10%). Besides a strong treatment effect, yield differences within locations and years, were attributed to both genotypic and genotype-by-treatment interactions. Pod yield under WW and WS conditions were closely related in both years (Patancheru, r 2 = 0.42 and r 2 = 0.50; Sadore, r 2 = 0.22 and r 2 = 0.23). By contrast, within location and treatment, pod and haulm yields were affected predominantly by genotype-by-year (G × Y) effects, especially under WS. Within treatment across locations and years, pod and haulm yields were mostly ruled by genotypic effects, which allowed identifying a group of entries with contrasting pod yield across locations under WS. However, genotype and genotype by environment (GGE) biplot analyses distinguished India from Niger, suggesting that the selection remains environment-specific and also revealed dissimilarity between years in Niger. A close relationship was observed between yield and pod growth rate (r 2 = 0.51), and partition (r 2 = 0.33) under WS conditions, whereas no significant relationship was found between yield under WS and SCMR, or specific leaf area (SLA). These results showing a close interaction between the environmental conditions and the genotypic response to intermittent drought shows the necessity to carefully choose environments that truly represent target environments. This is an important result in the current breeding context of marker-assisted recurrent selection or genome-wide selection. This work opens also new ways for the breeding of drought tolerant groundnut, by bringing new highly contrasting lines currently used for crossing and deciphering drought adaptation traits to better understand G × E interactions, while it challenges the relevance of long-time used surrogates such as SCMR or SLA.
The Bill and Melinda Gates Foundation (BMGF) funded Tropical Legumes (TL III) project was impleme... more The Bill and Melinda Gates Foundation (BMGF) funded Tropical Legumes (TL III) project was implemented in seven sub-Saharan Africa countries (Burkina Faso, Ghana, Mali, Nigeria, Ethiopia, Tanzania, and Uganda) and South Asia (India). Shortage of seed of improved varieties has been identified as the greatest hindrance to farmer adoption of new agricultural technologies developed through this project. This chapter compares the different approaches followed by different countries in the establishment of Multi-Stakeholder Platforms (MSPs) for supply of improved legume seed to farmers. Achievements from this initiative are mixed and multi-dimensional. The details herein provide the reader with insights on the level of success of innovation platforms in the different countries and implications for agricultural technology dissemination to smallholder farmers. Key achievements include strengthened linkages among various legume seed value chain actors, participation of several cadres of seed ...
Designed to address the issues that limit the access of small-scale farmers in sub-Saharan Africa... more Designed to address the issues that limit the access of small-scale farmers in sub-Saharan Africa to quality, affordable seed of the crops on which they depend for food security and livelihoods, this collection of articles describes successful principles for and experiences in community-based seed production. Among other things, the manuscripts analyze current seed production systems and models; propose ways to design successful community-based seed production schemes; describe proper seed production practices for selected cereals, vegetatively propagated plants, and other crops; and outline basic business practices for seed producers.
The study aimed to quantify the immediate effects of dietary diversification, food safety, and hy... more The study aimed to quantify the immediate effects of dietary diversification, food safety, and hygiene interventions on child undernutrition in four rural villages in Kongwa district of central Tanzania. One hundred mothers with their children of less than 24 months old were recruited for this study. The difference-indifference (DID) method was used to assess the effects of intensive intervention through a learning-by-doing process on the topic of aflatoxin free diversified food utilization and improved hygiene practices. Periodic anthropometric measurements were conducted on the 0th, 7th, 14th, and 21st days, and DID estimator showed the significant and positive average marginal effects of the intervention on Z-Scores being 0.459, 0.252, and 0.493 for wasting, stunting, and underweight, respectively. Notably, at the end of the study, the mean aflatoxin M 1 level in urine samples decreased by 64% in the intervention group, while it decreased by 11% in the control group. The study provides quantitative evidence on intensive 21-day training for mothers incorporating integrated technologies yielded positive impacts on their children's nutritional outcomes.
This article highlights 12 years (2007-2019) of research, achievements, lessons learned, challeng... more This article highlights 12 years (2007-2019) of research, achievements, lessons learned, challenges and gaps in discovery-to-delivery research in legumes emanating from three projects, collectively called Tropical Legumes (TL) with a total investment of about US$ 67 million funded by the Bill & Melinda Gates Foundation. These projects were implemented by three CGIAR centres (ICRISAT, CIAT and IITA) together with 15 national agricultural research system partners in sub-Saharan Africa and South Asia. The TL projects together with some of their precursors and complementary projects from other agencies, facilitated the development of 266 improved legume varieties and the production of about 497,901 tons of certified seeds of the target legume crops in the focus countries. The certified seeds have been planted on about 5.0 million ha by more than 25 million smallholder farmers in the 15 countries and beyond, producing about 6.1 million tons of grain worth US$ 3.2 billion. Furthermore, the projects also trained 52 next generation scientists that included 10 women, by supporting 34 Masters degrees and 18 PhD degrees. 1 | INTRODUC TI ON The Tropical Legumes (TL) projects supported by the Bill & Melinda Gates Foundation (BMGF) were jointly implemented by three CGIAR Centres (ICRISAT, CIAT and IITA) together with 15 national agricultural research system (NARS) partners in select focus countries
There are several hurdles to ensure sustainable seed production and consistent flow of improved l... more There are several hurdles to ensure sustainable seed production and consistent flow of improved legume varieties in sub-Saharan Africa (SSA) and South Asia (SA). The unreliable demand, autogamous nature of most of the grain legumes, and slow variety replacement rate by smallholder farmers do not provide strong incentive for private seed companies to invest in legume seed business. Unless a well thought-out and comprehensive approach to legume seed delivery is developed, current seed shortages will continue, eroding emerging market opportunities. The experiences reported here are collated through a 10-year partnership project, the Tropical Legumes in SSA and SA. It fostered innovative public-private partnerships in joint testing of innovative market-led seed systems, skills and knowledge enhancement, de-risking private sector initiatives that introduced in new approaches and previously overlooked entities in technology delivery. As new public and private seed companies, individual seed entrepreneurs and farmer organizations emerged, the existing ones enhanced their capacities. This resulted in significant rise in production, availability and accessibility of various seed grades of newly improved and farmer demanded legume varieties in the target countries. K E Y W O R D S decentralized seed production and supply, improved legume variety innovative legume seed systems, multi-stakeholder platform, sub-Saharan Africa and South Asia, variety promotion How to cite this article: Rubyogo J-C, Akpo E, Omoigui L, et al. Market-led options to scale up legume seeds in developing countries: Experiences from the Tropical Legumes Project.
Groundnut is an important food and oil crop in the semiarid tropics, contributing to household fo... more Groundnut is an important food and oil crop in the semiarid tropics, contributing to household food consumption and cash income. In Asia and Africa, yields are low attributed to various production constraints. This review paper highlights advances in genetics, genomics and breeding to improve the productivity of groundnut. Genetic studies concerning inheritance, genetic variability and heritability, combining ability and trait correlations have provided a better understanding of the crop's genetics to develop appropriate breeding strategies for target traits. Several improved lines and sources of variability have been identified or developed for various economically important traits through conventional breeding. Significant advances have also been made in groundnut genomics including genome sequencing, marker development and genetic and trait mapping. These advances have led to a better understanding of the groundnut genome, discovery of genes/variants for traits of interest and integration of marker-assisted breeding for selected traits. The integration of genomic tools into the breeding process accompanied with increased precision of yield trialing and phenotyping will increase the efficiency and enhance the genetic gain for release of improved groundnut varieties.
Legumes are important components of sustainable agricultural production, food, nutrition and inco... more Legumes are important components of sustainable agricultural production, food, nutrition and income systems of developing countries. In spite of their importance, legume crop production is challenged by a number of biotic (diseases and pests) and abiotic stresses (heat, frost, drought and salinity), edaphic factors (associated with soil nutrient deficits) and policy issues (where less emphasis is put on legumes compared to priority starchy staples). Significant research and development works have been done in the past decade on important grain legumes through collaborative bilateral and multilateral projects as well as the CGIAR Research Program on Grain Legumes (CRP-GL). Through these initiatives, genomic resources and genomic tools such as draft genome sequence, resequencing data, large-scale genomewide markers, dense genetic maps, quantitative trait loci (QTLs) and diagnostic markers have been developed for further use in multiple genetic and breeding applications. Also, these mega-initiatives facilitated release of a number of new varieties and also dissemination of on-the-shelf varieties to the farmers. More efforts are needed to enhance genetic gains by reducing the time required in cultivar development through integration of genomics-assisted breeding approaches and rapid generation advancement.
Aflatoxin-lysine (AFB-lys) adduct levels in blood samples collected from 230 individuals living i... more Aflatoxin-lysine (AFB-lys) adduct levels in blood samples collected from 230 individuals living in three districts of Malawi (Kasungu, Mchinji, and Nkhotakota) and aflatoxin B (AFB) levels in groundnut and maize samples collected from their respective homesteads were determined using indirect competitive enzyme-linked immunosorbent assay (IC-ELISA) methods. AFB-lys adducts were detected in 67% of blood samples, with a mean concentration of 20.5 ± 23.4 pg/mg of albumin. AFB was detected in 91% of groundnut samples and in 70% of maize samples, with mean AFB levels of 52.4 and 16.3 μg/kg, respectively. All participants of this study reported consuming maize on a daily basis and consuming groundnuts regularly (mean consumption frequency per week: 3.2 ± 1.7). According to regression analysis, a frequency of groundnut consumption of more than four times per week, being female, and being a farmer were significant (p < 0.05) contributors to elevated AFB-lys adduct levels in the blood. Th...
Th is study was conducted to determine the interaction between chickpea genotypes with the enviro... more Th is study was conducted to determine the interaction between chickpea genotypes with the environment (GxE) on the yield stability and adaptability of desi type chickpea genotypes (Cicer arietinum L.). Seventeen chickpea genotypes were evaluated for two cropping years (2012/2013-2013/2014) at four locations i.e., eight environments (locations x years combination). Chickpea grain yield was significantly (p<0.01) affected by genotypes, the environments and GxE interaction, indicating that the varieties and the test environments were diverse. GxE was further partitioned by principal component axes. The first two principal components cumulatively explained 53.1% of the total variation, of which 32.7% and 20.4% were contributed by IPCA1 and IPCA2, respectively. This implies that the interaction of 17 chickpea genotypes with eight environments was predicted by the first two principal components. AMMI1 biplot analysis showed five adaptive categories of genotypes based on similarities in their performance across environments. The AMMI2 biplot generated using genotypes and environmental scores for the first two IPCAs revealed positioning of the five genotype groups (GC) into four sectors of the biplot. Among them, two genotypes in GC 5 (G5 and G11) exhibited high yields across environments, low IPCA1 scores, low AMMI stability value (ASV) and yield stability index (YSI). G5 was released as a new variety, 'Dimtu' and registered in the Official Varieties Catalogue of Ethiopia, 2016.
In Zambia, groundnut products (milled groundnut powder, groundnut kernels) are mostly sold in und... more In Zambia, groundnut products (milled groundnut powder, groundnut kernels) are mostly sold in under-regulated markets. Coupled with the lack of quality enforcement in such markets, consumers may be at risk to aflatoxin exposure. However, the level of aflatoxin contamination in these products is not known. Compared to groundnut kernels, milled groundnut powder obscures visual indicators of aflatoxin contamination in groundnuts such as moldiness, discoloration, insect damage or kernel damage. A survey was therefore conducted from 2012 to 2014, to estimate and compare aflatoxin levels in these products (n = 202), purchased from markets in important groundnut growing districts and in urban areas. Samples of whole groundnut kernels (n = 163) and milled groundnut powder (n = 39) were analysed for aflatoxin B 1 (AFB 1) by competitive enzyme-linked immunosorbent assay (cELISA). Results showed substantial AFB 1 contamination levels in both types of groundnut products with maximum AFB 1 levels of 11,100 μg/kg (groundnut kernels) and 3000 μg/kg (milled groundnut powder). However, paired t test analysis showed that AFB 1 contamination levels in milled groundnut powder were not always significantly higher (P > 0.05) than those in groundnut kernels. Even for products from the same vendor, AFB 1 levels were not consistently higher in milled groundnut powder than in whole groundnut kernels. This suggests that vendors do not systematically sort out whole groundnut kernels of visually poor quality for milling. However, the overall contamination levels of groundnut products with AFB 1 were found to be alarmingly high in all years and locations. Therefore, solutions are needed to reduce aflatoxin levels in such under-regulated markets.
It is not known which aflatoxigenic species are present in Zambia. Therefore, soil samples were c... more It is not known which aflatoxigenic species are present in Zambia. Therefore, soil samples were collected during May to June 2012, at the end of the growing season in Eastern Province, from 399 farmers' fields that had been planted to groundnut (Arachis hypogaea L.) in Nyimba, Petauke, Mambwe, and Chipata Districts. Population densities of Aspergillus spp. were estimated by plating 10−3 soil dilutions on modified dichloran rose Bengal (MDRB) media. To test for toxigenicity, colonies were randomly selected from MDRB dilution plates, single-spored, transferred to vials with yeast extract sucrose (YES) liquid media, and grown for a week at room temperature. Agra Strip ® lateral flow cards were then used to test the filtered extracts, from the YES cultures, for total aflatoxin at 4 and 20 parts per billion (ppb). We identified Aspergillus flavus (small and large sclerotia strains), A. parasiticus, A. niger, A. nomius, A. oryzae, A. tamarii, and A. terreus. 100% of the S-strain A. fl...
A 3-year comprehensive analysis of aflatoxin contamination in peanut butter was conducted in Zamb... more A 3-year comprehensive analysis of aflatoxin contamination in peanut butter was conducted in Zambia, sub-Saharan Africa. The study analyzed 954 containers of 24 local and imported peanut butter brands collected from shops in Chipata, Mambwe, Petauke, Katete, and Nyimba districts and also in Lusaka from 2012 to 2014. For analysis, a sample included six containers of a single brand, from the same processing batch number and the same shop. Each container was quantitatively analyzed for aflatoxin B1 (AFB1) in six replicates by using competitive enzyme-linked immunosorbent assay; thus, aflatoxin contamination level of a given sample was derived from an average of 36 test values. Results showed that 73% of the brands tested in 2012 were contaminated with AFB1 levels >20 μg/kg and ranged up to 130 μg/kg. In 2013, 80% of the brands were contaminated with AFB1 levels >20 μg/kg and ranged up to 10,740 μg/kg. Compared with brand data from 2012 and 2013, fewer brands in 2014, i.e., 53%, h...
Background Throughout the world, and particularly in Africa, budgets for national agricultural re... more Background Throughout the world, and particularly in Africa, budgets for national agricultural research systems (NARS) are suffering severe cuts. In this context, it is impossible for programs to research all crop and livestock enterprises across all agroecologies. Nor is it possible to pursue a full range of strategic, applied and adaptive research for each priority problem.
Rising food and nutritional insecurity threatens the livelihoods of millions of poor people, part... more Rising food and nutritional insecurity threatens the livelihoods of millions of poor people, particularly in sub-Saharan Africa. Vegetable and legume production and consumption are a potent mechanism for small-scale, disadvantaged farmers to obtain the required nutrients in their diets and to generate much-needed income through trade. Vegetables and legumes are key sources of nutrients and health-promoting phytochemicals, providing higher micronutrient contents and a wider spectrum of essential compounds to meet nutritional and health needs than other food sources. Diversifying diets with vegetables and legumes is a cheaper, surer, and more sustainable way to supply a range of nutrients to the body and combat malnutrition and associated health problems than other approaches that target only a single or a few nutritional factors. Furthermore, vegetables and legumes often accompany staple crops in meals, and most staple crops are less palatable without vegetable or legume accompaniments. As a growing world population demands more and higher quality foods, and as environmental problems such as soil degradation, water scarcity, biodiversity loss, and climate change become more acute, the need for innovative vegetable and legume research solutions to improve food and nutritional security cannot be overemphasized.
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