Papers by Eskandar Moghimipour
Purpose: To investigate the effect of co-administration of two absorption enhancing bile salts, s... more Purpose: To investigate the effect of co-administration of two absorption enhancing bile salts, sodium glycocholate (NaGc) and sodium salicylate (NaSal), on insulin absorption via intestinal targeted delivery system. Methods: Insulin (10 IU/kg), associated with and without absorption enhancers (5 % enhancer solution of NaGc or NaSal), was administered to the duodenum, jejunum, and ileum part of the diabetic rat's gastrointestinal (GI) tract by surgical technique. The insulin absorbed from the GI tract was evaluated by its hypoglycemic effect at 45 and 60 min post-administration. Results: The results showed that insulin formulations containing NaGc or NaSal administered into the duodenum and with little quantity in the jejunum decreased blood glucose levels, compared to the reference formulations (p < 0.05). It was also observed that formulations containing cellulose acetate phthalate (CAP) protectors and enhancers may protect insulin during transit through the stomach for 18...
Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease, 2020
Cisplatin is used as a chemotherapy drug in the treatment of various types of cancer. Mitochondri... more Cisplatin is used as a chemotherapy drug in the treatment of various types of cancer. Mitochondrial dysfunction, oxidative stress and inflammation have been identified as major mechanisms of cisplatin nephrotoxicity. The present study investigated the protective effects of pure gallic acid and nanoparticle gallic acid nanoparticles (nano-gallic acid) on cisplatin induced nephrotoxicity. Nano-gallic acid was prepared by double emulsions-solvent evaporation technique using Eudragit RS 100 polymer and polyvinyl alcohol as carrier. Then, the physicochemical characterization of the nanoparticles was examined. In the present study, renal mitochondria was isolated using different centrifugal methods. Our data indicated that the doses of 50 and 100 mg/kg gallic acid and 10 mg/kg nano-gallic acid significantly decreased mitochondrial reactive oxygen species (ROS) formation, mitochondrial membrane damage (ΔΨm), mitochondrial malondialdehyde (MDA), tumor necrosis factor alpha (TNF-α), interleukin 6 (IL-6) and significantly increased mitochondrial glutathione (GSH), mitochondrial superoxide dismutase (MnSOD), mitochondrial glutathione peroxidase (GPX) and mitochondrial catalase compared to the cisplatin treated group. Histopathological studies also confirmed biochemical tests. Finally, our results confirmed that the pure gallic acid and its nanoparticle improved renal oxidative stress, inflammation and mitochondrial dysfunction in acute nephrotoxicity induced by cisplatin in rat. Nano-gallic acid (10 mg/kg) was selected as the most effective dose. The findings of this study showed the superiority of nano-gallic acid against pure gallic acid. In conclusion, nano-gallic acid-loaded Eudragit-RS 100 as a novel antioxidant can be considered in the treatment of renal complications of cisplatin.
Bioscience Biotechnology Research Communications, 2017
The fi rst and main goal of root canal treatment is the elimination of microorganisms from the co... more The fi rst and main goal of root canal treatment is the elimination of microorganisms from the contaminated root canal system and providing an environment for the healing of periapical tissues. Instrumentation alone cannoteffectivelyclean the complex root canal system. Souse of irrigantsalong mechanicalpreparationis required. But no single solution is able to fulfi ll these actions completely. Chlorhexidineis one of the substances thatis usedas anirrigantin endodontics. It has broadantimicrobialspectrum, but itdoes not have theability todissolveorganictissues. It has been shownthat theaddition ofsurfactantin thesolution can increasesthe abilityof dissolvetissue.In this in vitro study tissue-dissolving capacity of sodium hypochlorite (5/25% and 2/5%), chlorhexidine (0/2%) and modifi ed chlorhexidine (chlorhexidine + benzalkonium chloride and chlorhexidine + sodium lauryl solphate) were compared.Tissue samples prepared from bovine pulp and each of the samples immersed for 20 minutes in each test solution (changing the solution every 2 minutes).The sampleswereweighedbefore and aftertesting. The weight difference divided by the initial weight of the tissue sample, multiplied by 100, was defi ned as the percentage of tissue solubility.NaOCl 5/25% was more solublethantheothersolutions. ExceptCHX 0/2% and salineno statistically signifi cant differences was found between the tissuedissolving properties of othersolutions and NaOCl 5/25%.The results of this study indicate that the use of 0/2%CHX+2%SLS as irrigant in endodontic can show similar effect with NaOCl 5/25% in the solubility.
Jundishapur Journal of Natural Pharmaceutical Products, May 28, 2012
ABSTRACT Aflatoxin B1 (AFB1) is a highly toxic and hepatocarcinogenic metabolite produced by Aspe... more ABSTRACT Aflatoxin B1 (AFB1) is a highly toxic and hepatocarcinogenic metabolite produced by Aspergillus species. Some natural products are known to kill fungi and destroy toxins and toxin-producing agents. The purpose of this study is to provide experimental data on the antifungal activity of cumin oils and their components that could be considered suitable for application in foods and drugs. The essential oil (EO) of Cuminum cyminum L. collected from Alborz Mountain, Iran, was obtained by hydro-distillation. The oil was analyzed by gas chromatography (GC) and chromatography/mass spectrophotometry (GC/MS). The antifungal activity of the oil was studied with regard to the inhibition of the growth of Aspergillus flavus PICC-AF39 , Aspergillus flavus PICC-AF24, Aspergillus parasiticus NRRL-2999 and Aspergillus niger. The minimal inhibitory (MIC) and minimal fungicidal (MFC) concentrations of the oil were determined. α-Pinene (29.2%), limonene (21.7%), 1,8-cineole (18.1%), linalool (10.5%), linalyl acetate (4.8%), and α-terpineole (3.17%) were the major components of the essential oil from C. cyminum L., and the oil showed a strong inhibitory effect on fungal growth. Essential oils could be safely used as preservatives in pharmaceuticals as well as health and food products to protect them against toxigenic fungal infections.
Journal of Colloid and Interface Science, 2010
Water/sucrose laurate/ethoxylated mono-di-glyceride/ethanol/peppermint oil microemulsion systems ... more Water/sucrose laurate/ethoxylated mono-di-glyceride/ethanol/peppermint oil microemulsion systems were formulated and characterized using electrical conductivity, dynamic viscosity, nuclear magnetic resonance, dynamic light scattering, small angle X-ray scattering and cryogenic transmission electron microscopy. The solubilization capacity of water in the oil is dependent on the surfactants and ethanol/oil mixing ratios (w/w). Static percolation phenomena were observed in these systems, and the water volume fraction percolation threshold was determined. A progressive transformation of the water-in-oil to bicontinuous and inversion to oil-in-water microemulsions occurs upon dilution with water, which was revealed by the determination of the diffusion coefficients of both oil and water inside the microemulsions. The diffusion coefficients of the surfactants at the interface of the microemulsions increase with increasing water volume fraction. The periodicity of the microemulsions increases linearly with increasing water volume fraction. In addition, the correlation length increases with water volume fraction to a certain value then decreases. Cryo-TEM images of the oil-in-water microemulsions revealed the presence of spheroidal droplets of up to 12 nm diameter.
Journal of Bioscience and Bioengineering, 2012
Chitosan film (CSF), 2-dimensional scaffold, was modified with dexamethasone (DEX) in the present... more Chitosan film (CSF), 2-dimensional scaffold, was modified with dexamethasone (DEX) in the present work via aminoalcohol forming reaction between amino group of chitosan and carbonyl group of DEX. Successful immobilization was identified by ATR-FTIR spectroscopy and, the immobilized amounts were determined by weighting methods (WM) and integrated area analysis method (AM) of HPLC. For experiments of cell culture of osteogenic differentiation, our results showed that DEX immobilization has more efficiency than the other group (such as both groups of CSF and CSF with free DEX, CSF/fDEX), which was demonstrated as indicated by cell image analysis and ALP activity assay. The results show, as AD-MSCs cultured on DEX-CSF, its shape of cell transformed to polygonal or cubical. The ALP activity assay indicates that of DEX-CSF increase about 3.5 folds than that of CSF's. The results suggest that immobilization of DEX can make chitosan scaffolds to induce differentiation of AD-MSCs toward osteoblastic lineage for bone tissue engineering application.
Energy & Fuels, 1998
Because the methods specified by regulatory agencies for the determination of the physicochemical... more Because the methods specified by regulatory agencies for the determination of the physicochemical properties of biodiesel can be laborious and expensive, the development of alternative methodologies represents a major breakthrough. Thus, low-field nuclear magnetic resonance (NMR) is an advantageous option because it is nondestructive and reduces the cost and time consumption. In this study, the partial least squares (PLS) regression method was used to create models that correlated the decay curves of the Carr-Purcell-Meiboom-Gill (CPMG) signal, obtained from low-field NMR equipment (2.2 MHz for 1 H), with the kinematic viscosity, specific mass and refractive index of biodiesel and their blends. Seventeen oilseeds diversified between edible and nonedible oils were utilized to synthesize the biodiesel and produce binary blends. Separately, multivariate calibration models were created only with biodiesel and blends with castor bean because these samples showed different tendencies from the others. The values of root mean square error of prediction (RMSEP) for the kinematic viscosity, specific mass and refractive index were equal to 0.1 mm 2 •s −1 , 3.7 kg•m −3 and 0.002, re
AAPS PharmSciTech, 2006
This research was aimed to characterize microemulsion systems of isopropyl palmitate (IPP), water... more This research was aimed to characterize microemulsion systems of isopropyl palmitate (IPP), water, and 2:1 Brij 97 and 1-butanol by different experimental techniques. A pseudoternary phase diagram was constructed using water titration method. At 45% wt/wt surfactant system, microemulsions containing various ratios of water and IPP were prepared and identified by electrical conductivity, viscosity, differential scanning calorimetry (DSC), cryo-field emission scanning electron microscopy (cryo-FESEM) and nuclear magnetic resonance (NMR). The results from conductivity and viscosity suggested a percolation transition from waterin-oil (water/oil) to oil-in-water (oil/water) microemulsions at 30% wt/wt water. From DSC results, the exothermic peak of water and the endothermic peak of IPP indicated that the transition of water/oil to oil/water microemulsions occurred at 30% wt/wt water. Cryo-FESEM photomicrographs revealed globular structures of microemulsions at higher than 15% wt/wt water. In addition, self-diffusion coefficients determined by NMR reflected that the diffusability of water increased at higher than 35% wt/wt water, while that of IPP was in reverse. Therefore, the results from all techniques are in good agreement and indicate that the water/oil and oil/water transition point occurred in the range of 30% to 35% wt/wt water.
Journal of Contemporary Medical Sciences, 2019
Objectives: The aim of this study was to evaluate the efficacy of N. sativa with honey on treatme... more Objectives: The aim of this study was to evaluate the efficacy of N. sativa with honey on treatment of Kidney stone. Methods: A clinical trial was conducted on 42 patients who were referred to the urology clinic of Ganjavian Hospital, Dezful, Iran and were identified by a sonographist that they had kidney stones smaller than 6 mm, they were without hydronephrosis, and did not require medication. They were randomly assigned to control (n= 20) and treatment (n= 22) groups. 8 grams of NS and honey combination with one glass of warm water were given daily to the treatment group for one month. The control group did not receive medication. Both groups drank 6-8 glasses of water daily. Results: The rate of stone expulsion in the treatment group was significantly higher than the control group (P value = 0.0001). Conclusion: According to our results, it seems that NS with honey may have some beneficial effects in treatment of kidney Stone.
Acyclovir is used locally or systemically to treat cold sore and totally have no side effect. How... more Acyclovir is used locally or systemically to treat cold sore and totally have no side effect. However, this antiviral drug is not suitable for some people. The main aim of this study was to prepare and characterize shallomin liposomal hydrogel formulation and evaluation and comparison of dermal efficacy of this new formulation with acyclovir ointment on cold sores. Thin-Film Hydration was used to prepare liposome consisting of lecithin and cholesterol (molar ratio: 1:1). Morphology, size analysis, liposome encapsulation efficiency were determined. For preparation of hydrogel, Hydroxyethyl cellulose (HEC) was used. In vitro Skin permeation assay through abdominal region skin of male wistar rat was also determined. After preparing liposomal shallomin 1% in hydrogel a randomized controlled trial was performed on three groups (15 students in each groups) who showed cold sore within 24 hours. Liposomal shallomin gel, acyclovir and placebo was used every 6 hours for the first, second and ...
Smart Drug Delivery [Working Title], 2021
Cancer has been considered as the most cause of death in world. Employing of nanocarriers as drug... more Cancer has been considered as the most cause of death in world. Employing of nanocarriers as drug delivery systems provide a platform for delivering drugs with increasing the anti-cancer efficacy, enhancing bioavailability of drugs, reducing side effects, enhancing the circulation half-life of drugs, improving the distribution of drugs and overcoming drug resistance. A number of nanocarriers have been studied as drug delivery systems for improving the treatment of cancer including liposomes, micelle, polymeric nanoparticles, carbon nanotubes, dendrimers, solid lipid nanoparticle (SLN) and nanostructure lipid carrier (NLC). In order to enhance recognition and internalization of nanocarriers by the target tissues, their surfaces can be modified with targeting ligands such as integrins, transferrin, folic acid, polysaccharides and antibodies. In this chapter, we are going to introduce the targeted nanocarriers for improving the cytotoxic action of drugs with further attempt of decreasi...
Journal of the Iranian Chemical Society, 2020
Third-generation polyamidoamine (G3 PAMAM) dendrimer–digoxin (PAMAM-Dig) is prepared, and conjuga... more Third-generation polyamidoamine (G3 PAMAM) dendrimer–digoxin (PAMAM-Dig) is prepared, and conjugation is confirmed by 1H-NMR, Fourier transform infrared spectroscopy (FT-IR), and thermal analysis. Then, PAMAM-Dig impregnated polyvinyl chloride (PAMAM-Dig/PVC) film has been prepared by mixing PAMAM-Dig, PVC powder, and 2-nitrophenyl octyl ether (O-NPOE) as the plasticizer in tetrahydrofuran. Differential scanning calorimetry (DSC) evaluation shows the as-prepared film is thermally stable below 200 °C. The film is mounted above the powder as well as the essential oil of Thymus vulgaris. The Taguchi orthogonal array is used to optimize the experimental conditions for adsorption of volatile constituents. At optimized conditions, the obtained fingerprint was the same or better than static headspace sampling of the essential oil. The chromatogram pattern had also obtained by floating the fiber in the essential oil, and more components have observed. Film-to-film variations were less than 10%. All obtained patterns are compared with those of different solvent extracts of Thymus vulgaris. It is concluded that PAMAM-Dig/PVC film could apply for fingerprint analysis of herbs’ volatile and semi-volatile constituents with no need for prior extraction.
Jundishapur Journal of Natural Pharmaceutical Products, 2017
Background: The mosquitoes of Culicidae family are serious vectors of several tropical diseases, ... more Background: The mosquitoes of Culicidae family are serious vectors of several tropical diseases, such as malaria, filariasis, encephalitis, and nuisance. Control of mosquitoes and protection of people from their bites are of the most important ways to prevent transmitted diseases. Although the efficacy of N, N-diethyl-m-toluamide (DEET) is high and generally used as mosquito repellent, yet a number of biting diptera are tolerant to DEET. Furthermore, there are concerns about the safety of DEET and its allergic and toxic effects. Therefore, it is necessary to use other repellents like plant essential oils. Objectives: The aim of this work was to develop a safe repellent with a long-lasting protection based on micro-emulsion of eucalyptus essential oil. Methods: Eucalyptus globulus essential oil was obtained by water distillation in a Clevenger apparatus. The larvae of Culicidae were collected and adult mosquitoes reared for the repellency test. Preparation of micro-emulsions of Eucalyptus globulus essential oil was made by mixing the specified surfactant (Tween 80 and Span 20) and the appropriate amount of co-surfactant (propylene glycol) under the water titration method. The laboratory method, arm in cage, was used to estimate the time of protection of essential oil micro-emulsion against mosquitoes and DEET used as a standard repellent. Results: Physicochemical properties of formulated micro-emulsions were appropriate and suitable for topical application. Particle size of eucalyptus oil 15% w/w micro-emulsion was lowest. When applying eucalyptus oil micro-emulsion at concentrations of 5, 10, and 15% w/w, time of protection against mosquitoes were 82 ± 15.8, 135.7 ± 26.4, and 170.7 ± 26 minutes, respectively. These times of protection were similar to DEET at same concentrations and significantly more than eucalyptus essential oil. Conclusions: The formulated micro-emulsion of eucalyptus oil at a concentration of 15% w/w has potential repellency to the extent of DEET. It seems that nano-sized microemulsion is stable in terms of thermodynamics and kinetics. In conclusion, preparation of nano-sized microemulsion could delay the volatility of eucalyptus essential oil and volatile oil release from formulations and consequently increase protection time against mosquitoes.
Journal of Drug Delivery Science and Technology, 2018
Abstract The aim of the present study was to design and evaluate the combination of pH-dependent ... more Abstract The aim of the present study was to design and evaluate the combination of pH-dependent and time-dependent polymers for colon-specific drug delivery using response surface methodology (RSM). Theophylline was selected as a model drug and Eudragit® FS 30D (pH-dependent), Eudragit® RL 30D and Eudragit® RS 30D (as time-dependent) were used as coating polymers. Dissolution test was carried out in different pH media mimicking the transit of capsules from stomach to colon. The morphology of coated capsules was evaluated by scanning electron microscopy (SEM). In vivo studies were performed using fluorescent imaging in order to trace the movement of capsules in gastrointestinal (GI) tract. The results of in vitro studies showed that optimized formulation had suitable release profile at different pH values, which were in agreement with the predicted value by RSM. The SEM images revealed that surface coated capsules were smooth and also fluorescent imaging indicated that coated capsules disintegrated at the targeted colon region. These findings provide novel insights for the development of enteric coated capsule in order to specify drug delivery to colon.
Journal of Drug Delivery Science and Technology, 2018
In vivo evaluation of pH and time-dependent polymers as coating agent for colonic delivery using ... more In vivo evaluation of pH and time-dependent polymers as coating agent for colonic delivery using central composite design,
Jundishapur Journal of Natural Pharmaceutical Products, 2014
Development of polymeric in situ gels for controlled release of various drugs provides a number o... more Development of polymeric in situ gels for controlled release of various drugs provides a number of advantages over conventional dosage forms. Some important advantages of these smart systems are ease of application and reduced frequency of administration, as well as protection of the drug from environmental conditions.
Jundishapur Journal of Natural Pharmaceutical Products, 2015
Background: Apoptosis is an essential process for elimination of damaged and cancerous cells and ... more Background: Apoptosis is an essential process for elimination of damaged and cancerous cells and is also a desired effect for the anticancer activity of drugs. Cyclooxygenase-2 (COX-2) inhibitors including celecoxib are a class of drugs with chemopreventive and antiproliferative properties regardless of their routine anti-inflammatory effects. Objectives: The objective of this study was to evaluate the effect of celecoxib loaded nano-liposomes on the isolated rat hepatocytes and comparing the results with what were obtained from usual form of this chemopreventive agent. Materials and Methods: Freshly isolated rat hepatocytes were prepared by two step collagenase perfusion method and, following stabilization in rotary, were exposed to 0, 20, 40 and 100 μM of celecoxib in nano and usual form. Viability was obtained and apoptosis was determined by modified comet technique. Results: At high concentrations (40 and 100 μM), apoptosis was demonstrated and it was clearly more prominent in hepatocytes exposed to celecoxib loaded liposomes. Conclusions: Our results showed that celecoxib loaded liposomes robustly induced apoptosis, an action that can potentially make it more relevant than the usual form for chemopreventive strategies and also may raise concerns about its toxicity.
Ceramics International, 2009
The objective of the present study is to formulate and evaluate a new microemulsion (ME) for topi... more The objective of the present study is to formulate and evaluate a new microemulsion (ME) for topical delivery of griseofulvin. Methods: The solubilities of griseofulvin in different combinations of surfactant to cosurfactant (S/Co ratio) were determined. Accordingly, based on their phase diagrams, eight microemulsions were formulated and then evaluated with respect to their particle size, surface tension, viscosity, conductivity, zeta potential and stability. Their release behavior, Scanning Electron Microscopy (SEM), Differential Scanning Calorimetry (DSC), refractory index (RI), pH and Small-angle-X-ray scattering (SAXS) were also assessed. Results: The results indicated that the mean droplet size of the MEs ranged from 30.9 to 84.3 nm. Their zeta potential varied from-4.5 to-20.8. Other determined characteristics were viscosity: 254-381 cps, pH: 5.34-6.57, surface tension: 41.16-42.83 dyne.cm-1 , conductivity: 0.0442-0.111 ms.cm-1. The drug release was in the range of 22.4 to 43.69 percent. Also, hexagonal, cubic and lamellar liquid crystals were observed in SAXS experiments. Conclusion: It can be concluded that any alteration in MEs constituents directly affects their microstructure, shape, droplet size and their other physicochemical properties.
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Papers by Eskandar Moghimipour