Papers by ELIZABETH ROMERO
Biochemical and Biophysical Research Communications, 1998
for its activity (4). The posttranslational synthesis of The vitamin K-dependent g-glutamyl carbo... more for its activity (4). The posttranslational synthesis of The vitamin K-dependent g-glutamyl carboxylase Gla remains the only known biological process depengene was cloned from a rat liver genomic DNA library dent on vitamin K (1), hence the enzymatic mechanism and the structural organization of this gene was deterof this reaction is unique. mined. The carboxylase gene is 16.3 kb in length and Vitamin K-dependent proteins are known to have contains 15 exons and 14 introns. DNA sequence analycritical functions in blood coagulation, bone formation sis revealed that all 14 introns were U2-Type GT-AG and extracellular matrix calcification (5-7). Abnormal introns. A 2.8-kb DNA fragment corresponding to the embryogenesis of mice lacking the thrombin receptor 5-flanking region of the cloned gene demonstrated or factor V genes (8, 9) also suggests developmental transcriptional activity in a rat liver cell line that is functions of some of the vitamin K-dependent proteins. known to express the endogenous carboxylase gene.
Experimental Cell Research, 1998
Vitamin K-dependent carboxylase catalyzes the posttranslational modification of glutamate to ␥-ca... more Vitamin K-dependent carboxylase catalyzes the posttranslational modification of glutamate to ␥-carboxyglutamate (Gla) in its substrates, the vitamin K-dependent proteins (VKDPs). This modification is required for the activities of the VKDPs. Recent evidence demonstrates previously unrecognized roles for VKDPs as signaling molecules important in the regulation of cell growth, adhesion, and apoptosis, suggesting developmental functions for VKDPs and hence the carboxylase. The tissue distribution and functions of carboxylase in development are unknown. In this study, we isolated and characterized the full-length cDNA encoding the rat carboxylase and analyzed, at the cellular level, the expression of this gene in rat embryos by in situ hybridization. We demonstrate that the expression of this gene is highly regulated in a developmental and tissue-specific manner. Hepatocytes, the major site of synthesis of VKDPs of blood coagulation, express carboxylase mRNA late in gestation, in contrast to the central nervous system, mesenchymal, and skeletal tissues which express carboxylase mRNA early during rat embryogenesis. The tissue-specific temporal expression of the carboxylase gene during embryogenesis indicates that vitamin K-dependent carboxylation and the formation of Gla is developmentally regulated. These studies suggest that vitamin K-dependent carboxylation is an important modulator of embryonic VKDP function.
Novel, 28 kV, thermoplastic elastomeric (TPE) insulators were tested under IEC 1109 5000h (Annex ... more Novel, 28 kV, thermoplastic elastomeric (TPE) insulators were tested under IEC 1109 5000h (Annex C) conditions (slightly modified) for their long term performance. The modifications were made to more closely simulate the actual service environment. Various stresses applied include UV-A radiation, heat, rain, clear mist and salt fog, in addition to electrical. The insulators performed well showing only superficial changes
Novel, 28 kV, thermoplastic elastomeric (TPE) insulators were tested under IEC 1109 5000h (Annex ... more Novel, 28 kV, thermoplastic elastomeric (TPE) insulators were tested under IEC 1109 5000h (Annex C) conditions (slightly modified) for their long term performance. The modifications were made to more closely simulate the actual service environment. Various stresses applied include UV-A radiation, heat, rain, clear mist and salt fog, in addition to electrical. The insulators performed well showing only superficial changes
Applied Catalysis A-general, 2004
Respiratory Medicine, 2006
Archivos De Bronconeumologia, 2005
Archivos De Bronconeumologia, 2005
This study was designed to assess changes in skeletal muscle characteristics after 6 weeks&am... more This study was designed to assess changes in skeletal muscle characteristics after 6 weeks' high-intensity physical training of patients with moderate to severe chronic obstructive pulmonary disease (COPD) and to determine how the changes were related to improvements in exercise tolerance and health related quality of life (HRQL). Ten patients with a mean (SD) age of 60 (10) years and a forced expiratory volume in 1 second of 32% (9%) were enrolled. The effect of training on the 6-minute walk test, HRQL questionnaires, and skeletal muscles was examined for the 8 patients who completed the program. The structural and chemical characteristics of skeletal muscles before and after training were studied in vastus lateralis muscle biopsies. Training significantly modified the 6-minute walk test (P<.01), HRQL (P<.05), and citrate synthetase activity (P<.05). Changes in distances walked during the 6-minute walk test were significantly related to changes in the mean area of fibers (r=0.81). The results of this study indicate that 6 weeks of high-intensity physical training of COPD patients produces moderate changes in skeletal muscles which could partly explain improvements observed in exercise tolerance after respiratory rehabilitation.
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Papers by ELIZABETH ROMERO