Papers by Dr. Syed Mubashar Sabir
Soil health plays a pivotal role in ensuring sustainable agriculture, particularly in the face of... more Soil health plays a pivotal role in ensuring sustainable agriculture, particularly in the face of climate change challenges. This field study investigated the potential synergistic effects of biochar and farm yard manure (FYM) applications on soil properties, nutrient availability, cotton growth, and yield in cotton. Our findings highlight the promising impact of biochar and FYM, each applied at a rate of 5 t ha-1, on various aspects of soil and crop performance. Results indicated the substantial reduction in soil temperature, especially in the biochar + FYM treatment (27.2°C) compared to control treatment (37°C). This reduction in soil temperature created a favorable microclimate for cotton plants, mitigated heat stress and fostered healthier growth. Likewise, biochar + FYM combination showed a minor decrease in soil pH (pH 7.7) compared to control treatment (pH 8.1). Furthermore, the application of biochar + FYM significantly increased soil organic carbon (0.89%) and organic matte...
Climate change's threat to global agriculture, especially cotton production, has led to the a... more Climate change's threat to global agriculture, especially cotton production, has led to the adoption of mulching as a mitigation strategy. Organic mulches offer environmentally friendly benefits for cotton in challenging environments. A study evaluated various organic mulches' effects on soil properties, cotton yield, and quality in a semi-arid region with limited water and high temperature fluctuations. Results showed that wheat straw, rice, and sugarcane leaves straw maintained moderate soil temperatures (27.3°C to 27.4°C), unlike the control (41.6°C). Soil pH remained stable (7.9 to 8.1), and organic mulches raised soil carbon (0.68% to 0.72% vs. 0.51% control). Nutrient availability increased, with higher nitrogen (0.045% to 0.049%), phosphorus (6.2 mg kg−1 to 6.5 mg kg−1), and potassium (89 mg kg−1 to 92 mg kg−1) compared to control (0.028%, 5.6 mg kg−1, and 71 mg kg−1). Organic matter content rose (0.77% to 0.81%) versus the control (0.51%). Weed density decreased (4 t...
Brazilian Journal of Biology, Sep 1, 2021
Curcuma longa is an important dietary plant which possess several pharmacological activities, inc... more Curcuma longa is an important dietary plant which possess several pharmacological activities, including antioxidant, antimicrobial, anti-inflamatory, anticancer and anti clotting etc. The aim of the present study was to determine the phenolic profile of Curcuma longa and in vitro antioxidant and antidiabetic activities. In HPLC chromatogram of Curcuma longa rhizome extract 15 phenolic compounds were identified namely Digalloyl-hexoside, Caffeic acid hexoside, Curdione, Coumaric, Caffeic acid, Sinapic acid, Qurecetin-3-D-galactoside, Casuarinin, Bisdemethoxycurcumin, Curcuminol, Demethoxycurcumin, and Isorhamnetin, Valoneic acid bilactone, Curcumin, Curcumin-O-glucuronide respectively. The ethanolic extract displayed an IC 50 value of 37.1±0.3 µg/ml against alpha glucosidase. The IC 50 value of DPPH radical scavenging activity was 27.2 ± 1.1 μg/mL. It is concluded that ethanolic extract of Curcuma long is rich source of curcumin and contain several important phenolics. The in vitro antioxidant and alpha glucosidase inhibitory effect of the plant justifies its popular use in traditional medicine.
Clinical Phytoscience, Dec 1, 2020
Objective: To evaluate the antioxidant activities and to identify the bioactive compounds in hot ... more Objective: To evaluate the antioxidant activities and to identify the bioactive compounds in hot water extracts of Terminalia chebula fruit. Methods: The antioxidant activities were determined by DPPH assay, lipid peroxidation assay, iron chelation and total antioxidant assay. The phenolic composition was determined by HPLC-DAD. Human Rab8b Protein was used for the validation of compounds as anti-inflammation. String analysis for protein synergism was used. Results: The analysis of Terminalia chebula Retzius (Combretaceae) phenolics showed anti-inflammatory effect. The specific phenolic compositions were determined by high performance liquid chromatography (HPLC) and resulted in the identification of rutin, catechin, caffeic acid, gallicacid, ellagic acid, epicatechin, and quercetin as antioxidant compounds. Human Rab8b protein is selected for protein docking and all compounds except rutin showed good results. ADMET properties were checked by using AdmetSar and all seven compounds showed validation for AMET properties. The synergisms of compounds were analyzed by STRING analysis and our ligands shows strong binding with human Rab8b proteins. The aqueous extract was capable of inhibiting the lipid peroxidation in egg yolk phospholipid homogenate. The extract scavenged the 2,2-diphenyl-1-picrylhydrazyl radical (DPPH) (IC 50, 71.5 ± 2.1 μg/ml). The extract displayed the high metal chelation activities and reducing abilities on the phosphomolybdenum assay. Conclusions: It is concluded that extracts of T. chebula have good antioxidant and anti-inflammation activities and are rich in phenolics.
Ethnobotany Research and Applications
The medicinal orchid species of the Himalaya are of great importance due to their various medicin... more The medicinal orchid species of the Himalaya are of great importance due to their various medicinal properties. These orchids have been used for centuries by the local communities in the region for their medicinal benefits. The Himalayan orchids are particularly unique because they have adapted to the extreme environmental conditions of the high altitude and harsh weather. Unfortunately, due to habitat loss and overharvesting for commercial purposes, many orchid species are now threatened with extinction. The main objective of this research was to identify, document ethnomedicinal uses and find conservation status of medicinal orchids used by local inhabitants of western Himalayas, Azad Jammu & Kashmir, Pakistan. Methods: Interviews were conducted with 80 residents (30 women and 50 men) using a semi-structured questionnaire. Relative frequency of citation (RFC), use value (UV), fidelity level (FL), informant agreement ratio (IAR), plant parts value (PPV) and conservation status were used to express the results. Results: The research area is home of 18 orchids that are significant from an ethnobotanical perspective. The tuber (40%) and root (30%), followed by the rhizome (15%), were the most favored plant parts amongst the species that were recorded. The highest RFC was recorded for Habenaria intermedia (0.69) while Gymnadenia orchidis had the lowest 0.05. The UV was between 0.04 to 0.56. Habenaria intermedia was found to possess the highest UV (UV 0.56) whereas Cypripedium cordigerum had the loweest UV (0.04). The highest FL was found in Dactylorhiza hatagirea (68.75%) and Goodyera repens had the lowest FL (6.25%). The highest IAR value is 1.00, which contributes to digestive disorders, liver diseases, gout, urinary tract infection, antibacterial, analgesic, skin diseases, tuberculosis, and diabetes. The main plant parts used by the locals for ethnomedicine are tuber, root, rhizome, and leaves. The conservation status showed that 61 % species are vulnerable, 31% are endangered and 6% are near to threatened. According to ethnobotanical study, the native people of the researched area are knowledgeable about the practices of orchids, and both the plants and the native knowledge need to be protected.
Food and Agriculture Research Journal
The examination was done to decide the all-out phenolics, all-out flavonoids, and in-vitro antiox... more The examination was done to decide the all-out phenolics, all-out flavonoids, and in-vitro antioxidants of M. longifolia gathered from various areas (Rawalakot, Khaigala, Akhorban, Alisojal, Phirkot, Bhaikh, and Singola) of Poonch, Azad Jammu, and Kashmir. The watery concentrates of M. longifolia were surveyed for absolute phenolics, all-out flavonoids, restraint against thiobarbituric acid reactive species (TBARS) prompt by various star oxidants (10µM FeSo4 and 5µM sodium nitroprusside) in mice liver and brain homogenates, DPPH radical searching limit, iron-chelating power. The outcomes demonstrated that M. longifolia contained a generous level of phenolic (192.10±2.10 mg/g) and flavonoid compounds (4.23±0.03 mg/g) separately. Among various areas, the Alisojal indicated the most elevated antioxidant movement against lipid peroxidation prompted by iron in mice liver (IC50, 23.67±2.67 µg/ml) and brain (IC50, 13.85±0.85 µg/ml) and showed the most noteworthy cell reinforcement action a...
Coatings
Environmental pollution caused by heavy metal ions has become a major health problem across the w... more Environmental pollution caused by heavy metal ions has become a major health problem across the world. In this study, a selective colorimetric sensor based on starch functionalized silver nanoparticles (St-Ag NPs) for rapid detection of Hg2+ in real samples was developed. The environmentally friendly green approach was utilized to synthesize starch functionalized silver nanoparticles (St-AgNPs). A multi-technique approach involving UV-Vis absorption spectroscopy, Fourier transform infrared (FT-IR), X-ray diffraction (XRD), and scanning electron microscope (SEM) was used for the characterization of St-Ag NPs. These starch functionalized AgNPs were tested for the detection of heavy metals at 25 °C. The screening process revealed clear changes in the AgNPs color and absorption intensity only in the presence of Hg2+ due to the redox reaction between Ag0 and Hg2+. The color and absorption intensity of nanoparticles remain unchanged in the presence of all the other tested metals ion. The ...
Brazilian Journal of Biology, 2023
The present study was aimed to evaluate the antioxidant potential and inhibitory effect ofCannabi... more The present study was aimed to evaluate the antioxidant potential and inhibitory effect ofCannabis sativa and Morus nigra against lipid peroxidation in goat brain and liver homogenates. The formation of free radicals, highly reactive oxygen species (ROS) and reactive nitrogen species (RNS) is a normal metabolic process for cellular signaling and countering the antigens. However, they may cause serious damage if they produced at amplified tolls. In addition, metabolic disorders also serve as sources of these reactive species. Although the issue can be addressed through supplements and other phytochemicals. In this study, two plant species were evaluated for their biological potential by employing a spectrum of antioxidant assays. The antioxidant activity was performed by lipid peroxidation assay. The water extract prepared from leaves of Cannabis sativa and Morus nigra showed significant (P<0.05) inhibition as compared to control i.e., 522.6±0.06 and 659.97±0.03 µg/mL against iron...
Journal of Medicinal Plants Research, 2013
Antioxidants and dietary fibers are compact sources that are recommended for healthy diets in who... more Antioxidants and dietary fibers are compact sources that are recommended for healthy diets in whole grain foods. The antioxidant activity, total phenolic content (TPC) and lipid peroxidation was measured among 15 cultivars of wheat which are commonly used in Pakistan and the effect of salt stress was also evaluated. The results revealed that different concentrations of wheat (25, 50, 75, 150 and 300 µg/ml) in control and under salt stress (that is, electrical conductivity, 2 EC, 4 EC, 8 EC and 16 EC) showed antioxidant activity. The inhibitory concentration at 50% (IC50) values of different cultivars of wheat ranged from 22.9 to 27.01 µg/ml. On the basis of comparison of scavenging percentage of non-stress and stressed wheat groups at different doses of salt, we have concluded that LU26-CTR, PAS-90, BARS, NARC, WAFAQ, LISANI, SEHAR, MEHRAJ, GA-02 and SHAFAQ are salt tolerant varieties. The change of phenolic content suggests that wheat uses antioxidant properties of phenolics as a m...
Proline and glycine-betaine (GB) are the two most important organic osmolytes that hoard in a var... more Proline and glycine-betaine (GB) are the two most important organic osmolytes that hoard in a variety of plant species in response to environmental stresses such as extreme temperatures, drought, salinity, UV radiation and heavy metals. The fifteen cultivars of wheat which are commonly used in Pakistan were subjected to four salt treatments (0 EC, 2 EC, 8 EC, 16 EC) for four weeks and the contents of proline and glycine-betaine were estimated in order to find out the
international food research journal, 2019
Malnourishment is one of the major issues faced by people of mountain areas of Pakistan. Common b... more Malnourishment is one of the major issues faced by people of mountain areas of Pakistan. Common bean, being a rich source of protein, minerals, and nutraceutical compounds, is considered as a potential food to address the malnutrition problems in these areas. Azad Jammu and Kashmir is widely distributed with the indigenous unexplored landraces of common bean. In the present work, the nutritional profiling of 36 common bean ecotypes collected from the wide range of altitude, based on protein percentage, lysine contents, antioxidants activity, and total phenolic contents was carried out. Significant protein and lysine contents were found in the range of 13 24.5% and 4.4 7.4%, respectively. The DPPH free radical assay revealed very high antioxidants capacity of 67.8 97.8% with significant variation in the selected common bean germplasm. Total phenolic contents were determined using Folin-Ciocalteu reagent method and ranged from 68.9 to 110.3 mg GAE/100 g. Principal Component Analysis (...
Clinical Phytoscience, 2020
Objective To evaluate the antioxidant activities and to identify the bioactive compounds in hot w... more Objective To evaluate the antioxidant activities and to identify the bioactive compounds in hot water extracts of Terminalia chebula fruit. Methods The antioxidant activities were determined by DPPH assay, lipid peroxidation assay, iron chelation and total antioxidant assay. The phenolic composition was determined by HPLC-DAD. Human Rab8b Protein was used for the validation of compounds as anti-inflammation. String analysis for protein synergism was used. Results The analysis of Terminalia chebula Retzius (Combretaceae) phenolics showed anti-inflammatory effect. The specific phenolic compositions were determined by high performance liquid chromatography (HPLC) and resulted in the identification of rutin, catechin, caffeic acid, gallicacid, ellagic acid, epicatechin, and quercetin as antioxidant compounds. Human Rab8b protein is selected for protein docking and all compounds except rutin showed good results. ADMET properties were checked by using AdmetSar and all seven compounds show...
Journal of Animal and Plant Sciences, 2013
Plant growth promoting rhizobacteria (PGPR) colonize the plant roots and promote plant growth and... more Plant growth promoting rhizobacteria (PGPR) colonize the plant roots and promote plant growth and yield by different mechanisms. A study was conducted in a series of jar and pot trials where effect of four different PGPR isolates alone and in combination with kinetin on maize (Zea mays L.) was evaluated under controlled and natural conditions. Results of jar trial indicated that PGPR isolates had positive interaction with the kinetin and significantly increased shoot and root length. However, the synergistic effect of PGPR isolates and kinetin was significantly better. Data of pot trial further confirmed the results of jar trial where combined use of bacterial strains with kinetin increased plant height (53%), shoot fresh weight (48%), root length (50%), shoot dry weight (133%), root fresh weight (37%) and root dry weight (70%) as compared to the sole application of kinetin. While interaction of PGPR with kinetin also improved the nitrogen, phosphorus and potassium contents in plant tissues as compared to control. Study revealed that plant growth promoting capabilities of PGPR could be improved if they are supplemented with exogenous kinetin.
South African Journal of Botany, 2005
The hilly areas of Pakistan are very rich in medicinal plants. Among these, Hippophae rhamnoides ... more The hilly areas of Pakistan are very rich in medicinal plants. Among these, Hippophae rhamnoides ssp turkestanica L., Elaeagnus umbellata Thunb. and Crataegus oxyacantha L. are native plants of Northern Pakistan. Intra-and interspecific comparisons were made to investigate their morphological and biochemical composition using morpho-molecular techniques. The comparisons indicated large genetic and biochemical variation among the populations of each species and between the different species. The sodium dodecyl sulphate polycrylamide gel electrophoresis comparisons using total seed proteins of Hippophae rhamnoides, Elaeagnus umbellata and Crataegus oxyacantha indicated many common proteins and some variable proteins among the species. The biochemical comparison of Vitamin C, fatty oil and phytosterol content was also found to be variable among the species. Vitamin C and fatty oil contents were highest in Hippophae rhamnoides while phytosterol content was highest in Crataegus oxyacantha when compared with the other species tested in this study. Significant variation in morphological characters including plant height, number of branches per plant, number of thorns, plant canopy and berry weight was also observed in these three plant species.
International Journal of Scientific and Engineering Research
Proline, glycine-betaine (GB) total phenolics, Na+ and K+ contents their ratio and some oxidative... more Proline, glycine-betaine (GB) total phenolics, Na+ and K+ contents their ratio and some oxidative stress indices were studied in leaves of bread wheat cultivars namely Sehar-06, Lu-26, (salt-tolerant) and Miraj-08 and Wafaq-01 (salt-sensitive), grown under salinity treatments carried out in five levels (1< dS/m as control, 2, 4, 8, 16 dS/ m) via sodium chloride in order to find out the resistant cultivars of wheat against salt stress. Under high salt potency significant increase for activities of antioxidant enzymes such as ascorbate peroxidase (APX) and guiacol peroxidase (GPX), in salt tolerant varieties. On the other hand, in salt tolerant varieties, activity of (SOD) and (CAT) were not infected. Meanwhile, under salinity condition the activities of antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT) and (GPX) in sensitive cultivar was lower than control and no significant difference were recorded regarding (APX) activity. Salt tolerant varieties had more am...
Journal of Animal and Plant Sciences
Salt produces free radicals in the plants and these free radicals are scavenged by the antioxidat... more Salt produces free radicals in the plants and these free radicals are scavenged by the antioxidative capacity of the plant. Change in antioxidant activity, phenolic contents, lipid peroxidation, glycine betaine and proline contents were investigated in fifteen genotypes of wheat differing in salt tolerance. The results revealed that the aqueous extracts of wheat (300 µg/ml) in control and under salt stress (i.e. electrical conductivity, 2 EC, 4 EC, 8 EC and 16 EC) demonstrated antioxidant activity in DPPH radical assay and their increased scavenging percentage showed that AUQAB-2000, PIRSABAK-05, BAKHAR-2002 can be considered as salt tolerant varieties. The change of phenolic content suggests that wheat uses antioxidant properties of phenolics as a mechanism of salt stress. Whereas, the lipid peroxidation, proline and glycine betaine data has indicated that AUQAB-2000, PUNJAB-85, PIRSABAK-05, BAKHAR-2002, FARKHARE-SARHAD and KAGHAN-94 can be considered as salt tolerant varieties. Sa...
South African Journal of Botany, 2003
Sea buckthorn (Hippophae rhamnoides ssp. turkestanica) a member of family Elaeagnaceae, is a very... more Sea buckthorn (Hippophae rhamnoides ssp. turkestanica) a member of family Elaeagnaceae, is a very important multipurpose plant in the northern areas of Pakistan. It is an ideal plant for preventing soil erosion and land reclamation, can withstand extremes of temperature ranging from -43°C to 55°C and grows well under drought conditions and variable soil pH. The fruit is rich in nutrients and medicinal compounds such as vitamins, carotene, flavonoids, essential oil, carbohydrates, organic acids, amino acids, and soluble sugars. The plants are also important as fuel wood, fencing, fodder, soil erosion control, to make soil fertile by nitrogen fixation in roots and for the purpose of shelterbelts. In order to compare various populations of Sea buckthorn for morphological and biochemical composition, ten populations from different areas of northern Pakistan were compared using plant and fruit characters. The purpose of the investigation was to identify the variable populations for different valuable characteristics to develop improved varieties for commercial cultivation and easy fruit harvesting. The comparison indicated a significant amount of variability on morphological and biochemical basis. The variability will be utilised to develop commercial varieties of the plant utilising the conventional techniques of selection and hybridisation for economic activities on degraded land of mountainous regions of Pakistan.
American Journal of Plant Sciences, 2013
The activity of antioxidative enzymes system is affected by salt stress, chlorophyll content (CHL... more The activity of antioxidative enzymes system is affected by salt stress, chlorophyll content (CHL), leaf relative water content (RWC), Na + and K + contents, their ratio and some oxidative stress indices were studied in leaves of ten bread wheat cultivarsSehar-06, Lu-26, NARC-09, BARC-09 and Pirsbak-09' (salt-tolerant) and Kaghan-94, Rohtas-90, Soughat-90, Shaheen-94 and Zardana-89' (salt-sensitive), grown under salinity treatments carried out in five levels (1 < dS•m -1 as control, 2, 4, 8, 16 dS•m -1 ) via sodium chloride. Under high salt potency significant increase for activities of antioxidant enzymes such as ascorbate peroxidase (APX) and guiacol peroxidase (GPX), occurred in salt tolerant varieties. Meanwhile, under salinity condition the activities of antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT) and (GPX) in sensitive cultivar were lower than control. Regarding (APX) activity there was no significant difference between salinity and control situation. Under salt stress membrane stability index (MSI) of both cultivars were negatively influenced. Hydrogen peroxide (H 2 O 2 ) content of salinity sensitive cultivars was higher than control. Salt tolerant varieties had more amounts of K + content, K + and Na + ratio, relative water content, yield and chlorophyll under salt conditions, and sensitive ones recorded higher Na + content at tillering stage. The mechanism of salt stress might be achieved due to low lipid peroxidation, assumingly lower changes in membrane stability index and evasion of Na + combination and amplified activity of antioxidant enzymes.
Journal of Agricultural Science, 2013
The experiment conducted in the laboratory of Plant Breeding and Molecular Genetics, Faculty of A... more The experiment conducted in the laboratory of Plant Breeding and Molecular Genetics, Faculty of Agriculture, Rawalakot, Pakistan. Leaves of thirteen genotypes of sugarcane used for this experiment and were treated in an oven for heat stress. The treated leaves were then used for the estimation of phenol contents and the measurement of lipid peroxidation against heat stress. Minimum moisture loosed by CPF-237 and maximum moisture loss showed by CO-1148. Maximum phenol contents were observed in S-2003-US-778 showed high results (58.78 mg GAE/100ml) and minimum were Rb-72 and CO-1148 showed the lowest values (36.17 and 37.5 mg GAE/100ml). The higher heat tolerance of genotype, CPF-237 related to higher membrane stability, and maintenance of high fv/fm ratio under heat stress and lower lipid peroxidation of membranes. Hence, the relative tolerance of a genotype to heat stress as reflected by its lower lipid peroxidation, and higher membrane stability and pigment concentration, related to the levels of activity of its antioxidant enzymes. By using morphological parameters, S-2003-US-778 was significantly different from Rb-72. Based on biochemical data, the genotypes S-2003-US-778, S-2003-US-694, CPF-237 and Co-1148 are heat tolerant genotypes and thus can be successfully planted in warmer areas to alleviate the socioeconomic status of farmers.
Journal of Agricultural Studies, 2013
Leaves of thirteen genotypes of sugarcane were treated in the oven for heat stress. The treated l... more Leaves of thirteen genotypes of sugarcane were treated in the oven for heat stress. The treated leaves were used for the estimation of phenolic contents and the measurement of lipid peroxidation. Minimum moisture loss is showed by CSSG-668 and maximum moisture loss showed by NSG-45. Maximum phenolic contents were observed in HSF-240 (65.7 mg GAE/100 ml) and minimum results showed by Lho 83-153 and CP-43-33 (32.98 and 33.78 mg GAE/100 ml). Lho 83-153, HSF-242 and S-2002-US-133 were showed high tolerant against heat therefor, showed higher membrane stability, maintenance of high fv/fm ratio under heat stress and lower lipid peroxidation of membranes. Hence, the relative tolerance of genotype to heat stress as reflected by its lower lipid peroxidation, higher membrane stability and pigment concentration is related to the levels of activity of its antioxidant enzymes.
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Papers by Dr. Syed Mubashar Sabir